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      • KCI등재

        Response of Spodoptera frugiperda (Lepidoptera: Noctuidae) to salt‐stressed maize plants

        Wang Zai‐Ling,Haseeb Muhammad,Zhang Run‐Zhi 한국곤충학회 2021 Entomological Research Vol.51 No.11

        Salt-stressed maize is an economically important crop in many arid and semi-arid regions of the world where soil salinization is most common, and the invasive fall armyworm (FAW) Spodoptera frugiperda threatens global maize production. It thus poses a puzzle of whether FAW populations feeding on salt-stressed maize plants are becoming more or less invasive. Here, we evaluated the FAW response to salt-stressed maize plants by examining effects of salt-stressed maize plants on FAW survival, development and fecundity. We found a longer larval development time (salt-stressed, 12.9 ± 0.8 d; non-stressed, 11.3 ± 0.4 d), but unaffected survival rate and pupal mass accumulation in FAW feeding on salt-stressed maize plants. Moreover, the lifetime egg production of FAW females feeding on salt-stressed maize plants (633.5 ± 62.7 eggs) was reduced by nearly half compared with those feeding on non-stressed maize plants (1255.9 ± 70.3 eggs). Overall, FAW showed a negative response to salt-stressed maize plants. Due to limited population increase potential, FAW populations feeding on salt-stressed maize plants should pose less of a problem than ones feeding on non-stressed maize plants do. In practice, salt-stressed maize plants are quite common in arid and semi-arid regions where the relatively high-salinity groundwater is often used to irrigate maize plants, potentially limiting FAW population size. Thus, salt-stressed maize plants would contribute to practical applications of integrated pest management (IPM) strategies in controlling FAW.

      • KCI등재

        T-spline surface smoothing based on 1-ring neighborhood space angle

        Wang Aizeng,Li Ling,Chang Heng,Zhao Gang,Wang Wei,Yang Jiaming 한국CDE학회 2022 Journal of computational design and engineering Vol.9 No.4

        The prominent properties owned by T-spline, such as flexibility, continuity, local refinement, water tightness, make it extensively applied in Computer Aided Design (CAD)- and Computer Aided Engineering (CAE)-integrating scenarios. But the local fairness may dissatisfy in damaged areas or even on the entire surface of industry applications. Under these circumstances, local protrusion and sharp features appeared seriously affect the fairness of T-spline surfaces. Derived from the geometric properties of T-spline control points, we propose a smoothing algorithm based on the 1-ring neighborhood space angle to deal with local abruptions of T-spline surfaces. We also demonstrate the availability of the proposed algorithm through several experiments. Results show that this method is suitable for removing sharp features and smoothing unstructured T-spline surfaces.

      • Enantioselective Synthesis of S-Equol from Dihydrodaidzein by a Newly Isolated Anaerobic Human Intestinal Bacterium

        Wang, Xiu-Ling,Hur, Hor-Gil,Lee, Je Hyeon,Kim, Ki Tae,Kim, Su-Il American Society for Microbiology 2005 Applied and environmental microbiology Vol.71 No.1

        <B>ABSTRACT</B><P>A newly isolated rod-shaped, gram-negative anaerobic bacterium from human feces, named Julong 732, was found to be capable of metabolizing the isoflavone dihydrodaidzein to <I>S</I>-equol under anaerobic conditions. The metabolite, equol, was identified by using electron impact ionization mass spectrometry, <SUP>1</SUP>H and <SUP>13</SUP>C nuclear magnetic resonance spectroscopy, and UV spectral analyses. However, strain Julong 732 was not able to produce equol from daidzein, and tetrahydrodaidzein and dehydroequol, which are most likely intermediates in the anaerobic metabolism of dihydrodaidzein, were not detected in bacterial culture medium containing dihydrodaidzein. Chiral stationary-phase high-performance liquid chromatography eluted only one metabolite, <I>S</I>-equol, which was produced from a bacterial culture containing a racemic mixture of dihydrodaidzein. Strain Julong 732 did not show racemase activity to transform <I>R</I>-equol to <I>S</I>-equol and vice versa. Its full 16S rRNA gene sequence (1,429 bp) had 92.8% similarity to that of <I>Eggerthella hongkongenis</I> HKU10. This is the first report of a single bacterium capable of converting a racemic mixture of dihydrodaidzein to enantiomeric pure <I>S</I>-equol.</P>

      • SCIESCOPUSKCI등재

        GROUND OBSERVATIONS OF SPRITES AND OTHER TLES IN TAIWAN

        WANG YUN-CHING,HSU RUE-RON,SU HAN-TZONG,CHEN ALFRED BING-CHIH,LEE YI-JEN,KUO CHENG-LING,TSAY WEAN-SHUN,CHANG CHAN-KAO,WANG SHI-CHUN,LEE LOU-CHUANG,LIU TIE-YUE The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2

        Sprites, elves and blue jets are collectively denominated as the upper atmospheric transient luminous events (TLEs). They are recently discovered optical flashes between active thunderstorms and the ionosphere. In this report, a brief introduction to the most important characteristics of TLEs is given. Since 2001, scientists from the National Cheng Kung University have been performing yearly summer campaigns from various locations in Taiwan. The main achievements of their yearly campaign are presented.

      • Enhanced biosynthesis of dihydrodaidzein and dihydrogenistein by a newly isolated bovine rumen anaerobic bacterium

        Wang, Xiu-Ling,Shin, Kwang-Hee,Hur, Hor-Gil,Kim, Su-Il Elsevier 2005 Journal of biotechnology Vol.115 No.3

        <P><B>Abstract</B></P><P>A rod-shaped and Gram-positive anaerobic bacterium, named Niu-O16, which was isolated from bovine rumen contents, was found to be capable of anaerobically converting isoflavones daidzein and genistein to dihydrodaidzein (DHD) and dihydrogenistein (DHG), respectively. The metabolites DHD and DHG were identified using EI-MS and NMR spectrometric analyses. Stereoisomeric metabolites, which were separated on chiral stationary phase HPLC, were formed in equal amounts by the strain Niu-O16. Tautomerization reaction occurred on the B-ring of DHD and DHG seems to be attributed to the equal production of stereoisomeric metabolites. For the synthesis of DHD, the strain Niu-O16 showed an optimal pH range from 6.0 to 7.0 and completely reduced up to 800μM of daidzein to DHD with the initial OD<SUB>600nm</SUB>=1.0 and pH 7.0 for 3 days incubation. The strain Niu-O16, showed relatively faster reduction activity toward daidzein to produce DHD than the previously isolated human intestinal bacterium <I>Clostridium</I> sp. HGH6.</P>

      • DNA Microarray Probe Preparation by Gel Isolation Nested PCR

        Wang, Hong-Min,Ma, Wen-li,Huang, Hai,Xiao, Wei-Wei,Wang, Yan,Zheng, Wen-Ling Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.3

        To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared. The DNA microarray probes were collected by a novel method of nested PCR that was mediated by gel isolation of the first PCR products. This method was named GIN-PCR. The probes that were prepared by this GIN-PCR technique were used as subjects to fabricate a DNA microarray. The results showed that a wooden toothpick was superior to the other 3 methods, since this technique can steadily transfer the DNA bands as the template of the second PCR after the first PCR. A group of probes were successfully collected and DNA microarrays were constructed using these probes. Hybridization results demonstrated that this technique of DNA recovery and probe preparation was rapid, efficient, and effective. We developed a cost-effective and less labor-intensive method for DNA microarray probe preparation by nested PCR that is mediated by wooden toothpick transfer of the DNA bands in the gel after electrophoresis.

      • Hong Kong Chinese Women's Lay Beliefs about Cervical Cancer Causation and Prevention

        Wang, Linda Dong-Ling,Lam, Wendy Wing Tak,Wu, Joseph,Fielding, Richard Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

        Background: This study aimed to seek insights into Chinese women's lay beliefs about cervical cancer causal attributions and prevention. Materials and Methods: Twenty-three new immigrant adult women from Mainland China and thirty-five Hong Kong adult women underwent semi-structured in-depth interviews. Interviews were audio taped, transcribed and analyzed using a Grounded Theory approach. Results: This study generated three foci: causal beliefs about cervical cancer, perceived risk of cervical cancer, and beliefs about cervical cancer prevention. Personal risky practices, contaminated food and environment pollution were perceived as the primary causes of cervical cancer. New immigrant women more likely attributed cervical cancer to external factors. Most participants perceived cervical cancer as an important common fatal female cancer with increased risk/prevalence. Many participants, particularly new immigrant women participants, expressed helplessness about cervical cancer prevention due to lack of knowledge of prevention, it being perceived as beyond individual control. Many new immigrant participants had never undergone regular cervical screening while almost all Hong Kong participants had done so. Conclusions: Some Chinese women hold pessimistic beliefs about cervical cancer prevention with inadequate knowledge about risk factors. Future cervical cancer prevention programs should provide more information and include capacity building to increase Chinese women's knowledge and self-efficacy towards cervical cancer prevention.

      • KCI등재

        A Study on the Red Carpet Dress of Film Festivals in the Great China Region

        ( Ling Wang ),( Mi Suk Lee ) 한국패션비즈니스학회 2014 패션 비즈니스 Vol.18 No.3

        The purpose of this study is to establish the basic materials necessary for red carpet fashion design by examining the formativeness and fashion images of red carpet dresses at film festivals in the Great China Region. For the purpose of this study, research methods include a literature review on the origin and significance of red carpet dresses, the characteristics of film festivals in the Great China Region and their red carpet dresses as well as an analysis of the formative features and images of 615 red carpet dresses collected from each film festival official homepage, diverse media articles, and online search sites (www.google.com, www.hao123.com). The research finding can be summarized as follows: First, the formative features of red carpet dress designs were analyzed herein. It was found that the most frequently appearing type of silhouette was straight followed by hourglass and bulk in order. More specifically these included fit and flare, mermaid, trapeze, and slim in order. For the neckline styles, strapless was the most frequently seen followed by camisole, jewel, and one shoulder. Solid colors were more often seen than multiple colors. Bk, W, R, and YR were the most frequent main solid colors in order. Solid materials were frequent as well, such as soft and shiny materials. Non-patterned and unadorned styles were most frequent as for pattern types and details and trimmings. Second, the fashion images of red carpet dresses in the Great China Region were analyzed. The most frequent images were elegant, feminine, ethnic, modern, classic, avant-garde, others, mannish and sportive, in order.

      • Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

        Wang, Ling,Wang, Qiang,Li, He-Lian,Han, Li-Ying Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

        Aim and Background: Cervical cancer remains the third most common cancer in women globally after breast and colorectal cancer. Well-characterized biomarkers are necessary for early diagnosis and to predict metastatic progression and effective therapy. MiRNAs can regulate gene expression, cell growth, differentiation and apoptosis by targeting mRNAs for translational repression or degradation in tumor cells. The present study was conducted to assess expression of miR93, miR200a, RECK, MMP2, MMP9 in invasive cervical carcinoma, and analyze their clinical significance. Method: A total of 116 patients with invasive cervical carcinoma and 100 patients undergoing hysterectomy for benign lesions were retrospectively examined. Quantitative real-time PCR was performed to determine expression of miR93 and miR200a while RECK, MMP2, MMP9 and MVD were assessed by immunohistochemical staining. Results: Cervical carcinoma patients demonstrated up-regulation of miR-93, miR-200a, MMP2 and MMP9, with down-regulation of RECK as compared to benign lesion tissues. RECK was significantly inversely related to invasion and lymphatic metastasis. The 5-year survival rate for patients with strong RECK expression was significantly higher than that with weakly expressing tumors. Conclusion: MiR-93 and miR-200a are associated with metastasis and invasion of cervical carcinoma. Thus together with RECK they are potential prognostic markers for cervical carcinoma. RECK cooperating with MMP2, MMP9 expression is a significant prognostic factor correlated with long-term survival for patients with invasive cervical carcinoma.

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