Response of Spodoptera frugiperda (Lepidoptera: Noctuidae) to salt‐stressed maize plants

Wang Zai‐Ling,Haseeb Muhammad,Zhang Run‐Zhi 한국곤충학회 2021 Entomological Research Vol.51 No.11

Salt-stressed maize is an economically important crop in many arid and semi-arid regions of the world where soil salinization is most common, and the invasive fall armyworm (FAW) Spodoptera frugiperda threatens global maize production. It thus poses a puzzle of whether FAW populations feeding on salt-stressed maize plants are becoming more or less invasive. Here, we evaluated the FAW response to salt-stressed maize plants by examining effects of salt-stressed maize plants on FAW survival, development and fecundity. We found a longer larval development time (salt-stressed, 12.9 ± 0.8 d; non-stressed, 11.3 ± 0.4 d), but unaffected survival rate and pupal mass accumulation in FAW feeding on salt-stressed maize plants. Moreover, the lifetime egg production of FAW females feeding on salt-stressed maize plants (633.5 ± 62.7 eggs) was reduced by nearly half compared with those feeding on non-stressed maize plants (1255.9 ± 70.3 eggs). Overall, FAW showed a negative response to salt-stressed maize plants. Due to limited population increase potential, FAW populations feeding on salt-stressed maize plants should pose less of a problem than ones feeding on non-stressed maize plants do. In practice, salt-stressed maize plants are quite common in arid and semi-arid regions where the relatively high-salinity groundwater is often used to irrigate maize plants, potentially limiting FAW population size. Thus, salt-stressed maize plants would contribute to practical applications of integrated pest management (IPM) strategies in controlling FAW.

Overexpression of Hiwi Promotes Growth of Human Breast Cancer Cells

Wang, Da-Wei,Wang, Zhao-Hui,Wang, Ling-Ling,Song, Yang,Zhang, Gui-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

The Piwi subfamily comprises two argonaute (Ago) family proteins, which are defined by the presence of PAZ and Piwi domains, with well known roles in RNA silencing. Hiwi, a human Piwi subfamily member, has been shown to play essential roles in stem cell self-renewal and gametogenesis. Recently, accumulating reports have indicated that abnormal hiwi expression is associated with poorer prognosis of multiple types of human cancers, including examples in the breast. However, little is known about details of the oncogenic role of hiwi in breast cancers. In present study, we confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines at both mRNA and protein levels. Thus both RT-qPCR and Western blot data revealed significantly higher hiwi in intratumor than peritumor specimens, overexpression being associated with tumor size, lymph node metastasis and histological grade. Hiwi overexpression was also identified in breast cancer cell lines, MDA-MB-231 and MCF-7, and gain-of-function and loss-of-function strategies were adopted to identify the role of hiwi in the MCF-7 cell growth. Results demonstrated that hiwi expression in MCF-7 cells was significantly up- or down-regulated by the two strategies. We next evaluated the influence of hiwi overexpression or knockdown on the growth of breast cancer cells. Both cell count and colony formation assays confirmed promoting roles of hiwi in MCF-7 cells, which could be inhibited by hiwi specific blockage by siRNAs. In summary, the present study confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines, and provided e vidence of promotion by hiwi of cell growth. The results imply an oncogenic role of hiwi in breast cancers.

Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling

Ling Wang,Xuemei Zhang,Guangying Wu,Yuhong Qi,Jinghui Zhang,Jing Yang,Hong Wang,Wenchun Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.4

Streptococcus pneumoniae is a Gram-positive pathogen with high morbidity and mortality globally but some of its pathogenesis remains unknown. Previous research has provided evidence that aminopeptidase N (PepN) is most likely a virulence factor of S. pneumoniae. However, its role in S. pneumoniae virulence and its interaction with the host remains to be confirmed. We generated a pepN gene deficient mutant strain and found that its virulence for mice was significantly attenuated as were in vitro adhesion and invasion of host cells. The PepN protein could induce a strong innate immune response in vivo and in vitro and induced secretion of IL-6 and TNF-α by primary peritoneal macrophages via the rapid phosphorylation of MAPK and PI3K/AKT signaling pathways and this was confirmed using specific pathway inhibitors. In conclusion, PepN is a novel virulence factor that is essential for the virulence of S. pneumoniae and induces host innate immunity via MAPK and PI3K/AKT signaling.

The General Status of Soybean producbon Utilitation and Scientific Research in China

Wang, Jin Ling 한국콩연구회 1994 韓國콩硏究會誌 Vol.11 No.1

The present ordinary output of soybeans in China is around 10 million tons. Heilanjiang Province is the leading province of the national soybean production, and her 1993 soybean production area in 1993 was 3.3 million hectares and the amount of output was 5.5 million tons. Soybean produced in China is chiefly used to meet the domestic demand, and is taken as one of the chief sources of edible protein and fat. For a long time inhabitants in North and North East of China took steamed cakes made with 30% soybean flour and 70% sorghum or corn flour as staple food. Now soybean subsidiary food such as dofu is indispensable to the national wide Chinese people. Soybean meal is not only an important feed but is also an important commodity for exportation. In different regions of China farmer established different types of crop rotation systems. According to the sequence position of soybeans in the rotation systems five soybean cultivation regions have been classified : 1. Spring soybean region 2. North summer soybean region 3. South summer soybean region 4. Autumn soybean region 5. Multiple harvest soybean region. There are specific soybean ecotypes and specific soybean management methods in each of the regions. Soybean breeding program is widely carried out in China, especially in the North East where released cultivars derived from cross breeding have been replaced for five times. Collection, research and utilization of soybean germplasms is deeply considered and 20,000 accession of cultivated types and 2.000 accessions of wild soybeans have been registered. Soybean mosaic virus is prevalent in China. In the North East of China there exists the epidemic of cyst nematode. In north-eastern part of North East frog-eye leaf spot and root rot, in southern provinces soybean rust and bacterial pustule are the major soybean diseases. Two kinds of pod borer, aphids and black stem fly are the important soybean insect pest in China. In cooperation with the farmers Chinese soybean experts of Heilonjiang Province have established $quot;three procedures on ridge culture$quot; and $quot;Yong Chang model$quot; mechanized high yield soybean management methods, and favorable results have been obtained on area more than one hundred thousand hectares.

Analysis on Casual Relationship between International Arbitration and Foreign Direct Investment A Comparative Analysis of China, Korea, Japan, Mongolia and United States

Wang Mei-Ling(왕메이령),Choi Chang-Hwan(최창환) 한국국제상학회 2016 國際商學 Vol.31 No.4

This paper investigated whether the international arbitration can promote FDI or not, using the comparison analysis of empirical data from 2001 to 2012 in China, Korea, Japan, Mongolia and United States. The empirical results showed promoted as International Arbitrations then FDI would be promoted. If GDP, Export, Import and Unemployment increased, then the FDI decreased. On the other hand, FDI, Unemployment, Import increased, International Arbitrations increased too. But the more Export and GDP are the less international arbitration cases. As the growth of continuous increase in the scale of economy and trade, the arbitration system acting as the settlement system to solve commercial transaction disputes is more emphasised. It can futher prompt the develpment of economy and trade & social develoment of related countries and contribute to the growth of economic cooperation.

Efficient Similarity Search Techniques with a Real-Time Approximate Analysis in Streaming Database

Ling Wang,Tie Hua Zhou,Kyung Ah Kim,Eun Jong Cha,Keun Ho Ryu 보안공학연구지원센터 2013 International Journal of Multimedia and Ubiquitous Vol.8 No.4

Sensitive Distance Estimates Technique Analysis for Continuously K-Nearest Neighbors Query in Multi-Stream Processing

Ling Wang,Tie Hua Zhou,Kyung Joo Cheoi,Kwang Deuk Kim,Keun Ho Ryu 보안공학연구지원센터 2012 International Journal of Multimedia and Ubiquitous Vol.7 No.2

SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells

Wang, Hong-Ling,Lu, Ren-Quan,Xie, Su-Hong,Zheng, Hui,Wen, Xue-Mei,Gao, Xiang,Guo, Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such as aging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function as a tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovarian carcinoma cells and its potential mechanism. Materials and Methods: Expression of SIRT7 in ovarian cancer cell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression were constructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate the biological function of SIRT7 in ovarian cancer cells. Results: SIRT7 demonstrated a higher level in ovarian cancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reduced ovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand, up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced change in apoptosis-related molecules and subunits of the NF-${\kappa}B$ family. Conclusions: In the present study, our data indicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.

Doxorubicin induces early embryo apoptosis by inhibiting poly(ADP ribose) polymerase.

Wang, Qing-Ling,Sun, Shao-Chen,Han, Jemesbond,Kwak, Yong-Chul,Kim, Nam-Hyung,Cui, Xiang-Shun Dr. J.G. Delinassios 2012 In vivo Vol.26 No.5

<P>The effect of Doxorubicin, a widely used chemotherapeutic agent, on early mouse embryonic development has not been previously characterized.</P>

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

Wang, Ting-Ting,Wang, Shao-Kang,Huang, Gui-Ling,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an $IC_{50}$ of $70.7{\pm}1.72{\mu}M$ at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.