Comparative analysis of bovine maternal corpus luteum microRNAs with aberrant and normal developed cloned fetus at late gestation

Xiaohu Su,Shenyuan Wang,Guangqi Gao,Xinyu Zho,Lidong Han,Guanghua Su,Jiaqi Zhang,Wanfu Bai,Xiuying Wang,Guangpeng Li,Li Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background The development efficiency of cloned cattle is extremely low (< 5%), most of them were aborted at late gestation. Based on our previous studies, some recipient cows with a cloned fetus would present as engorged uterine vessels and enlarged umbilical vessels randomly. Abortion involves both maternal and fetal factors. Objective Our aim was to explore this phenomenon by microRNAs expression profile analysis of maternal corpus luteum (CL), which was related to pregnancy maintenance. Methods The present study provided the comparison of maternal CL miRNAs expression of abnormally and normally developed cloned bovine fetus at late gestation (~ 210 days) using RNA-Seq technology. Results We selected two abnormally pregnant cows (abnormal group, AG) and three normally pregnant cows (normal group, NG) and acquired valid reads of 9317,261–12,327,185 (~ 84.53–91.28%) from five libraries. In total, we identified 981 conserved miRNAs and 223 novel miRNAs. 1052 miRNAs were co-expressed, 124 miRNAs were uniquely expressed in AG, and 93 miRNAs were uniquely expressed in the NG. Compared with NG, 11 were significantly overexpressed, and 22 were downregulated (p < 0.05) at AG among 1052 co-expressed miRNAs. The differentially expressed miRNAs-targeted genes were further analyzed by Gene Ontology and KEGG pathway analysis. Notably, the steroid biosynthesis pathway was a significantly enriched term (p < 0.01), which may affect the secretion of progesterone. Conclusion Our research suggested that abnormal miRNAs expression of bovine maternal CL may affect the pregnant status at late gestation.

RO 및 NF막에서의 부식질에 의한 Fouling 및 미량오염물질의 제거

왕창근,차준철,이용현,김수동,류주환 대한상하수도학회 2004 상하수도학회지 Vol.18 No.1

This study investigated the phenomena of membrane fouling by NOM and the effect of the fouling on removal of micro-pollutants. NOM has a great effect on decline of permeate flux. Permeate flow rate was reduced by 88% in RO and 34.8% in NF for 323hr operation period. Removal rate of UV_(254), is 87.4% in RO and 78.5% in NF and removal rate of DOC is 42.7% in RO and 32.9% in NF for 2㎎/l humic acid. Removal efficiency of the micmpollutants by the RO and NF membranes fouled by humic acid was mostly lower than that by the new membrane. The concentration polarization which affects the flux and the rejection was thought to occur in the active layer of the membrane, as the membrane was getting fouled.

Netflow 기반 종단 간 Flow 모니터링

김성수,송왕철 제주대학교 공과대학 첨단기술연구소 2006 尖端技術硏究所論文集 Vol.17 No.1

As the next generation internet (NGI) is supporting High-speed in Gbps rate with the appearance of advanced network technologies, various applications that require high data rates such as video conferencing, telemedicine etc. have been experimented and operated. By using high speed application services, many kinds of problems can be generated but we cannot easily grasp their reasons. However, if the user monitors the end-to-end flow data, he can find them more accurately. Until now, we have found out the fact that systems which can network-widely monitor end-to-end flow have not be developed yet, only simple systems which can monitor user's indivisual flow data at just one node are developed. In this study, we suggest and materialize a system which can analyze bandwidth in real time by searching routing paths and measuring packet transfer rate between end-to-end flow data. By using the system, user can find out routing path for the end-to-end flow and also present data easily rate by some measured value. It is expected that the network manager can analyze and fix the problem, hence, improving the quality of service.

IEEE 802.11b/g 무선랜 기반 SOHO환경에서의 트래픽 측정 및 분석

정우철,김성수,송왕철 제주대학교 공과대학 첨단기술연구소 2006 尖端技術硏究所論文集 Vol.17 No.1

802.11 b and 802.11g are both IEEE standards but they have different performances. Today, SOHO(Small Office Home Office) environment requires wireless channels. Therefore, in this paper, the general performance of wireless channels in SOHO environment is analyzed. Basically, we do the measurements for wireless environment between the host and the host AP. At first, we test the streaming service and then we confirm the service difference to use VOIP phone. We do several experiments to estimate the performance of the services in wireless environment of SOHO.

불포화 레시틴을 이용한 나노리포좀 개발에 관한 연구

강기춘,이청희,왕수환,이천일,표형배,정노희 한국공업화학회 2004 응용화학 Vol.8 No.1

Nowadays many products using nano-technology have been released and nano-technology would be more prospected in all kinds of industries especially cosmetic manufacturing and ingredient fields. In cosmetics, the saturated lecithin, one of the main surfactants to make liposome, has been used for its stability but it has been substituting by unsaturated lecithin which has excellent skin affinity and penetration. So we studied to develop nano-liposome below 50㎚ with unsaturated lecithin. To make stable nano-liposome, many factors including solvent like propylene glycol, pH balance, homogenizing pressure and liposome stabilizer like surfactant are important especially stearate series surfactants have positive effect in liposome size and long term stability. But in liposome with saturated lecithin, surfactant has negative effect and appropriate amount of oil should be used to be stable. So we could conclude that unsaturated lecithin is suitable than saturated lecithin to make nano-liposome.

Reformability evaluation of blasting-enhanced permeability in in situ leaching mining of low-permeability sandstone-type uranium deposits

Wang Wei,Liang Xuanyu,Niu Qinghe,Wang Qizhi,Zhuo Jinyi,Su Xuebin,Zhou Genmao,Zhao Lixin,Yuan Wei,Chang Jiangfang,Zheng Yongxiang,Pan Jienan,Wang Zhenzhi,Ji Zhongmin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8

It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.

Overexpression of Long Non-Coding RNA HOTAIR Promotes Tumor Growth and Metastasis in Human Osteosarcoma

Wang, Bo,Su, Yun,Yang, Qun,Lv, Decheng,Zhang, Weiguo,Tang, Kai,Wang, Hong,Zhang, Rui,Liu, Yang Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.5

Human osteosarcoma usually presented a high tendency to metastatic spread and caused poor outcomes, however, the underlying mechanism was still largely unknown. In the present study, using a series of in vitro experiments and an animal model, we investigated the roles of HOX antisense intergenic RNA (HOTAIR) during the proliferation and invasion of osteosarcoma. According with our results, HOTAIR was commonly overexpressed in osteosarcoma, which significantly correlated with advanced tumor stage, highly histological grade and poor prognosis. In vitro and in vivo experiments demonstrated that knockdown of HOTAIR could notably suppress cellular proliferation, inhibit invasion and decrease the secretion of MMP2 and MMP9 in osteosarcoma. Collectively, our results suggested that HOTAIR might be a potent therapeutic target for osteosarcoma.

SNP Discovery from Transcriptome of Cashmere Goat Skin

Wang, Lele,Zhang, Yanjun,Zhao, Meng,Wang, Ruijun,Su, Rui,Li, Jinquan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associated with genes affecting economic traits. Validation of single nucleotide variations in a whole-transcriptome sequencing is critical for understanding the information of molecular genetics. In this paper, we aim to develop a large amount of convinced single nucleotide polymorphisms (SNPs) for Cashmere goat through transcriptome sequencing. In this study, the transcriptomes of Cashmere goat skin at four stages were measured using RNA-sequencing and 90% to 92% unique-mapped-reads were obtained from total-mapped-reads. A total of 56,231 putative SNPs distributed among 10,057 genes were identified. The average minor allele frequency of total SNPs was 18%. GO and KEGG pathway analysis were conducted to analyze the genes containing SNPs. Our follow up biological validation revealed that 64% of SNPs were true SNPs. Our results show that RNA-sequencing is a fast and efficient method for identification of a large number of SNPs. This work provides significant genetic resources for further research on Cashmere goats, especially for the high density linkage map construction and genome-wide association studies.

Moving Mass Actuated Reentry Vehicle Control Based on Trajectory Linearization

Su, Xiao-Long,Yu, Jian-Qiao,Wang, Ya-Fei,Wang, Lin-lin The Korean Society for Aeronautical and Space Scie 2013 International Journal of Aeronautical and Space Sc Vol.14 No.3

The flight control of re-entry vehicles poses a challenge to conventional gain-scheduled flight controllers due to the widely spread aerodynamic coefficients. In addition, a wide range of uncertainties in disturbances must be accommodated by the control system. This paper presents the design of a roll channel controller for a non-axisymmetric reentry vehicle model using the trajectory linearization control (TLC) method. The dynamic equations of a moving mass system and roll control model are established using the Lagrange method. Nonlinear tracking and decoupling control by trajectory linearization can be viewed as the ideal gain-scheduling controller designed at every point along the flight trajectory. It provides robust stability and performance at all stages of the flight without adjusting controller gains. It is this "plug-and-play" feature that is highly preferred for developing, testing and routine operating of the re-entry vehicles. Although the controller is designed only for nominal aerodynamic coefficients, excellent performance is verified by simulation for wind disturbances and variations from -30% to +30% of the aerodynamic coefficients.

[P8-116] Inhibitory Effect of Kalanchoe thyrsifolra Extract on Pancreatic Lipase

Su-Mi Ahn,Jae-Won Huh,Wang-Su Lee,Ki-Moon Park 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10