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The Expression of Genes Related to Egg Production in the Liver of Taiwan Country Chickens
Ding, S.T.,Ko, Y.H.,Ou, B.R.,Wang, P.H.,Chen, C.L.,Huang, M.C.,Lee, Y.P.,Lin, E.C.,Chen, C.F.,Lin, H.W.,Cheng, Winston Teng Kuei Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1
The purpose of this study was to detect expression of genes related to egg production in Taiwan Country chickens by suppression subtractive hybridization. Liver samples of mRNA extraction from two Taiwan Country chicken strains (L2 and B), originated from the same population but with very distinct egg production rates after long-term selection for egg and meat production respectively. Two-way subtraction was performed. The hepatic cDNA from the low egg production chickens (B) was subtracted from the hepatic cDNA from the high egg production strain (L2). The reversed subtraction (L2 from B) was also performed. The resulting differentially expressed gene fragments were cloned and sequenced. We sequenced 288 clones from the forward subtraction and 96 clones from the reverse subtraction. These genes were subjected to further screening to confirm the differential expression between the two genetic breeds of chickens. The apolipoprotein B (apoB) was expressed to a greater extent in the liver of the L2 than in the B line chickens. The 5-aminoimidazole- 4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (PURH) was expressed to a greater extent in the liver of the B than in the L2 strain chickens. We demonstrated that both apoB and PURH were more highly expressed in the liver than that in other tissues (muscle, ovary, and oviduct) in laying Taiwan Country chickens. Taken together, these data suggest that after the selection for egg production, expression of apoB and PURH genes were also changed. Whether the changed expression of these genes is directly related to egg production is not known, but these two genes may be useful markers for egg laying performance in Taiwan Country chickens.
Li, L.,Lin J. B.,Luo, W. Y. 한국지능정보시스템학회 2022 한국지능정보시스템학회 학술대회논문집 Vol.2022 No.6
Despite the importance of artificial intelligence (AI) technologies in improving customer relationships, AI usage in enabling customer relationship management (CRM) capabilities and in turn improving CRM performance has not yet been investigated. Drawing on dynamic capability theory, this study investigates the impact of AI usage on customer relationship management performance and tests the mediating effect of customer relationship management capabilities. We test our core proposition and theory-driven research model using data collected from a sample of 193 e-commerce enterprises in China. The empirical results indicate that artificial intelligence usage positively impacts customer relationship management performance and customer relationship management capabilities play a positive mediating role in the relationship between them. Thus, this paper contributes to IS research with an eloquent theoretical explanation and strong empirical evidence on why e-commerce enterprises deploy AI initiatives to improve their CRM capabilities and performance.