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Age-dependent immune response in pigs against foot-and-mouth disease virus in vitro
( Jae-hee Roh ),( Ngoc Anh Bui ),( Hu Suk Lee ),( Vuong Nghia Bui ),( Duy Tung Dao ),( Thanh Thi Vu ),( Thuy Thi Hoang ),( Kyoung-min So ),( Seung-won Yi ),( Eunju Kim ),( Tai-young Hur ),( Sang-ik Oh 한국축산학회 2021 한국축산학회지 Vol.63 No.6
Foot-and-mouth disease, one of the most contagious diseases in cloven-hoofed animals, causes significant economic losses. The pathogenesis of foot-and-mouth disease virus (FMDV) infection is known to differ with age of the animals. In this study, we aimed to reveal the difference in immunological response in the initial stage of FMDV infection between piglets and adult pigs. Peripheral blood mononuclear cells (PBMCs) were isolated from 3 piglets (8 weeks old) and 3 pigs (35 weeks old) that were not vaccinated against FMDV. O-type FMDV (2 × 10<sup>2</sup> median tissue culture infectious dose) was inoculated into porcine PBMCs and the cells were incubated at 37.0℃ under 5% CO<sub>2</sub> for various time periods (0, 1, 3, 6, 12, 24, and 48 h). The total RNA was obtained from the FMDV-inoculated PBMCs after each time point, and the virus titer was investigated in these RNA samples. Furthermore, dynamics of mRNA expression of the six tested cytokines (interferon [IFN]-α, IFN-γ, interleukin [IL]-6, IL-8, IL-10, and tumor necrosis factor [TNF]-α) in FMDV-inoculated porcine PBMCs were evaluated by time-series analysis to determine the differences, if any, based on the age of the pigs. The PBMCs of piglets contained the highest quantity of FMDV mRNA at 6 hours post-inoculation (hpi), and the PBMCs of pigs had the highest quantity of FMDV mRNA at 3 hpi. The mean cycle threshold-value in the PBMCs steadily decreased after the peak time point in the piglets and pigs (6 and 3 hpi, respectively). The dynamics of mRNA expression of all cytokines except TNF-α showed age-dependent differences in FMDV-inoculated PBMCs. The mRNA expression of most cytokines was more pronounced in the piglets than in the pigs, implying that the immune response against FMDV showed an age-dependent difference in pigs. In conclusion, within 48 hpi, the 8-week-old piglets responded more rapidly and were more sensitive to FMDV infection than the 35-week-old pigs, which could be associated with the difference in the pathogenesis of FMDV infection among the pigs. These results provide valuable insights into the mechanisms underlying the age-dependent differences in immune response in pigs against FMDV infection.
Alisha Wehdnesday Bernardo Reyes,Tran Xuan Ngoc Huy,Son Hai Vu,Trang Thi Nguyen,Wongi Min,Hu Jang Lee,Suk Kim 한국예방수의학회 2020 예방수의학회지 Vol.44 No.4
We investigated the cytotoxic potential of three different commercially available absorbent feminine hygiene products and one transdermal patch using direct contact and extract exposure methods. Two different cell lines were used - mouse fibroblast L929 and normal human skin fibroblast CCD-986sk cell lines. The test samples were extracted using three different methods in accordance to International Organization for Standardization (ISO). Viability of cells was analyzed using MTT assay and morphology of the cells were also observed using phase contrast microscopy. Overall, the direct contact method using L929 cells showed that all the test samples had no toxic effect when exposed to extracts for 1 h. For the exposure method, no toxic effect was observed in both L929 and CCD986sk cells incubated with all the test samples regardless of the extraction methods used.
e-MITOCHONDRIA RESEARCH FOR SYSTEMS BIOLOGY AND PROTEOMICS
Hyun Joo(주현),Jae boum Youm(염재범),Taeho Kim(김태호),Nari Kim(김나리),Won sun Park(박원선),Sunghyun Kang(강성현),Dang Van Cuong,Hyoung kyu Kim(김형규),Tran Min Khoa,Vu Thi hu,Hyunju Kim(김현주),Hyejin Moon(문혜진),Hyunsuk Le 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10