Effect of Prunella vulgaris Labiatae Extract on Innate Immune Cells and Anti-metastatic Effect in Mice

Jun Beom Lee,Tae Bong Kang,Sang-Hoon Choi,Uiyoung Lee,Ae-Jung Kim,Chang-Jin Jeong,Hak-Cheon Lee,Yong-Sun Cho,Jong-Gun Won,Jong-Cheol Lim,Taek Joon Yoon 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.1

Ability of water extract from Prunella vulgaris Labiatae to stimulate immune system and inhibit tumor metastasis in mice was assessed. In experimental lung metastasis, prophylactic intravenous (i.v.) administration of water extract from P. vulgaris significantly inhibited lung metastasis in a dose-dependant manner. Peritoneal macrophages stimulated with P. vulgaris produced various cytokines such as tumor necrosis factor (TNF)-α and interlukin (IL)-12 as well as induced tumoricidal activity. In an assay for natural killer (NK) cell activity, i.v. administration of P. vulgaris significantly augmented NK cytotoxicity. The depletion of NK cells by injection of rabbit anti-asialo GM1 serum abolished the inhibitory effect of P. vulgaris on lung metastasis of colon26-M3.1 cells. These data demonstrate that P. vulgaris activate innate immune system to inhibit the growth of foreign materials including tumor cells in mice.

대용량 에너지 흡수장치가 적용된 중간연결장치의 유지보수 방안 연구

선의영(UiYoung Seon),김영규(Young-gyu Kim),정구인(KooiN Jung),노제택(JaeTeak Noh),이현태(HyunTae Lee),김한영(Hanyoung Kim) 한국철도학회 2018 한국철도학회 학술발표대회논문집 Vol.2018 No.10

철도차량의 안전도 수준은 다른 교통수단보다도 높은 안전성을 갖추고 있지만, 높은 안전성에도 불구하고 시스템 오류 및 휴먼 에러로 인한 철도차량 사고는 종종 발생하고 있다. 철도는 근본적으로 제동거리가 길고, 철도차량 자체의 중량이 무겁기 때문에 철도차량 사고가 발생하면 큰 사고로 이어지게 된다. 사고에 따른 피해를 최소화하기 위해서는 사고 예방이 가장 최우선적으로 이루어져야 한다. 예기치 않는 사고 발생 시 인적 및 물적 피해를 최소화와 승차감 향상을 위해 철도차량의 연결장치에 대용량 에너지 흡수장치의 적용하였을 때 검사외 시험 및 유지보수 방안에 대하여 고찰하였다.

Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells

Hwang, Jun-Ha,Han, Uiyoung,Yang, Miso,Choi, Yonghyun,Choi, Jonghoon,Lee, Jong-Min,Jung, Han-Sung,Hong, Jinkee,Hong, Jeong-Ho Elsevier 2019 ACTA BIOMATERIALIA Vol.86 No.-

<P><B>Abstract</B></P> <P>In regenerative medicine, the generation of therapeutic stem cells and tissue engineering are important for replacing damaged tissues. Numerous studies have attempted to produce cellular components that mimic the native tissue for gaining optimal function. Particularly, the extracellular matrix (ECM) composition plays an important role in cellular functions including determining the fates of mesenchymal stem cells (MSCs). Here, we evaluated the osteogenic effects of a nanofilm in which oppositely charged polyelectrolytes were alternately adsorbed onto the cell surface to create an artificial ECM environment for single MSCs. Interestingly, nanofilm composed of collagen (Col) and alginate (AA) showed relatively high stiffness and MSCs coated with the Col/AA nanofilm showed increased osteogenic differentiation efficiency compared to other nanofilm-coated MSCs. Further analysis revealed that the Col/AA nanofilm coating stimulated osteogenesis by activating transcriptional coactivators with the PDZ binding motif through extracellular signal-related kinase and p38 MAPK signaling. This nano-sized cellular coating will facilitate the development of nanotechnology for controlling cellular functions and advance stem cell-based clinical applications for regenerative medicine.</P> <P><B>State of Significance</B></P> <P>In this study, we developed an artificial cellular nano-environment formed by multilayer nanofilms. We demonstrated that the nanofilms introduced to mesenchymal stem cells (MSCs) stimulate osteogenic differentiation by regulating intracellular signaling. Among the various nanofilm combinations, the induction of osteogenic gene transcription in collagen (Col) and alginate (AA) film-coated MSCs was the most pronounced compared to that on other nanofilms. A minimum number of Col/AA nanofilm bilayers (n = 2) was required for effective induction of MSC osteogenic differentiation. In addition, we observed the correlation between the promoting effect of osteogenic differentiation and stiffness of the nanofilm. Our results may be useful for developing a cell coating model system widely applicable in bioengineering and regenerative medicine.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Catalytic, Conductive, and Transparent Platinum Nanofiber Webs for FTO-Free Dye-Sensitized Solar Cells

Kim, Jongwook,Kang, Jonghyun,Jeong, Uiyoung,Kim, Heesuk,Lee, Hyunjung American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.8

<P>We report a multifunctional platinium nanofiber (PtNF) web that can act as a catalyst layer in dye-sensitized solar cell (DSSC) to simultaneously function as a transparent counter electrode (CE), i.e., without the presence of an indium-doped tin oxide (ITO) or fluorine-doped tin oxide (FTO) glass. This PtNF web can be easily produced by electrospinning, which is highly cost-effective and suitable for large-area industrial-scale production. Electrospun PtNFs are straight and have a length of a few micrometers, with a common diameter of 40–70 nm. Each nanofiber is composed of compact, crystalline Pt grains and they are well-fused and highly interconnected, which should be helpful to provide an efficient conductive network for free electron transport and a large surface area for electrocatalytic behavior. A PtNF web is served as a counter electrode in DSSC and the photovoltaic performance increases up to a power efficiency of 6.0%. It reaches up to 83% of that in a conventional DSSC using a Pt-coated FTO glass as a counter electrode. Newly designed DSSCs containing PtNF webs display highly stable photoelectric conversion efficiencies, and excellent catalytic, conductive, and transparent properties, as well as long-term stability. Also, while the DSSC function is retained, the fabrication cost is reduced by eliminating the transparent conducting layer on the counter electrode. The presented method of fabricating DSSCs based on a PtNF web can be extended to other electrocatalytic optoelectronic devices that combine superior catalytic activity with high conductivity and transparency.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-8/am400179j/production/images/medium/am-2013-00179j_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am400179j'>ACS Electronic Supporting Info</A></P>

고속 영상데이터 암호화에 적합한 SES Alarmed 링크 암호동기 방식

김형락,이훈재,권대훈,박의영,Kim, HyeongRag,Lee, HoonJae,Kwon, DaeHoon,Pak, UiYoung 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.12

CCSDS 규격은 국제 우주 통신 분야에서 하나의 규격으로 폭넓게 사용되고 있다. 이 규격에서 암호화는 계층별로 고유의 암호 프로토콜을 수행해 통신 채널에서 보안 요구사항을 만족하도록 하고 있고, 동기화를 위해서는 암호화된 데이터의 시작부에 암호화 동기를 삽입하여 수행한다. 그러나 예외적인 상황(타이밍 지터링, 시스템의 비정상적인 종료 등)의 발생 시 수신 장비는 데이터를 수신하더라도 정상적인 데이터를 복원할 수 없게 되는 문제점을 가지고 있다. 본 논문에서는 송수신 도중에 문제가 발생할 경우 송신측에 SES Alarm 신호를 전송하여 정상적인 송수신이 이루어지도록 하는 SES Alarmed 링크 암호동기 방식을 제안하고, 성능 분석을 통하여 SES Alarm 신호를 위한 최적의 조건을 제시하였다. CCSDS Standard is widely used in international space telecommunication area. In this standard, Encryption is realized using a unique hierarchical encryption protocol and satisfied security requirements of communication channels. For synchronization, encryption sync is attached in the beginning of encrypted data. But exceptional case(timing jittering, abnormal system shutdown, etc.) is occurred, receiving equipment cannot decrypt received data. In this paper, we propose a SES Alarmed link encryption synchronization method for sending warning signal to the transmitter when some problems have been occurred during the transmission and we also suggest optimum conditions for SES Alarm signal through performance analysis.

비밀 중간키를 이용한 소프트웨어적 전력분석공격 방어대책

박영구,김형락,이훈재,한덕찬,박의영,Park, YoungGoo,Kim, HyeongRag,Lee, HoonJae,Han, DeokChan,Pak, UiYoung 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.12

전력분석공격은 알려진 값과 마스터키로부터 생성된 숨겨진 값을 입력 받아 연산하는 시점에서 이루어진다. 연산 결과값과 연산 중에 측정된 전력신호의 상관도를 분석하여 숨겨진 값을 찾아내고, 이 찾아낸 값으로 부터 마스터키를 추정할 수 있다. 그러나 전력분석을 가능하게 하는 조건을 바꾸어, 알려진 값과 연산자를 숨기거나, 숨겨진 값으로부터 마스터키의 추정이 불가능하게 하거나, 연산 결과 값과 전력신호간의 상관도를 매우 낮게 하면 전력분석공격이 매우 어렵게 된다. 이와 같은 전력분석공격을 어렵게 하는 조건을 적용한 비밀 중간키를 이용한 소프트웨어적 방어 대책을 제안한다. Correlation power analysis attack takes place at the point of operating a known value and a hidden value from a master key as an input. The hidden value can be found by analyzing a correlation between the result value and the measured power signal during the operation, witch makes it possible to estimate the master key from the hidden value. However, the correlation power analysis attack can be very difficult by changing the conditions that make the power analysis possible, when the known value and the operation is hidden, when it is impossible to estimate the master key from the hidden value, or when the correlation between the result value and the power signal is considerably lowered. Therefore this study proposes a software countermeasure using a secrete intermediate key to witch these conditions are applied.

도시철도차량 연결기의 진동 특성 측정을 통한 승차감 분석 및 평가

정구인(Kuin Jung),김영규(Younguy Kim),김태도(Taedo Kim),선의영(Uiyoung Seon),김기남(Kinam Kim),이진호(Jinho Lee) 한국철도학회 2018 한국철도학회 학술발표대회논문집 Vol.2018 No.5

일반적으로 승차감이란 차내 환경이 승객에게 주는 심리적․ 생리적 반응을 말한다. 이 요인에는 진동, 가속도, 소음, 온습도, 통풍, 빛의 량, 압력, 냄새 등 모든 인자가 포함된다. 그 중 차량의 주행에 따라서 발생하는 진동이나 가속도에 따라서 기인하는 승객의 반응을 좁은 의미의 승차감으로서 정의하고 있다. 차량의 주행에 따르는 승차감을 검토할 경우, 보통, 진동 승차감, 곡선 통과시의 승차감, 가감속시의 승차감 및 진자 차량의 승차감의 4종류로 분류하고 있다. 진동승차감은 가장 일반적인 승차감으로 차량의 주행에 따르는 상하, 좌우, 전후 진동 가속도를 평가지표로서 사용한다. 본 연구에서는 도시철도차량 구형 전동차의 연결기와 신형 전동차 연결기와의 진동 특성 측정을 통하여 승차감 지수 및 감쇠특성을 도출하여 연결기 모델별 상호 비교분석을 수행하였다.

Multilayer Nanofilms via Inkjet Printing for Stabilizing Growth Factor and Designing Desired Cell Developments

Choi, Moonhyun,Park, Hee Ho,Choi, Daheui,Han, Uiyoung,Park, Tai Hyun,Lee, Hwankyu,Park, Juhyun,Hong, Jinkee Wiley - VCH Verlag GmbH & Co. KGaA 2017 Advanced Healthcare Materials Vol.6 No.14