Anti-Inflammatory Effect of Rosa rugosa Flower Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Tursun, Xirali,Zhao, Yongxin,Talat, Zulfiya,Xin, Xuelei,Tursun, Adila,Abdulla, Rahima,AkberAisa, Haji The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Rosa rugosa Thunb, a deciduous shrub of the genus Rosa, has been widely used to treat stomach aches, diarrhoea, pain, and chronic inflammatory disease in eastern Asia. In recent years, our research team has extensively studied the Rosa rugosa flower extract, and specifically undertook pharmacological experiments which have optimized the extraction process. Our methods have yielded a standard extract enriched in phenolic compounds, named PRE. Herein, we expand our efforts and evaluated the anti-inflammatory activity of PRE on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages. PRE significantly inhibited production of nitric oxide (NO), prostaglandin $E_2(PGE_2)$, tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6, and interleukin $1{\beta}$ (IL-$1{\beta}$), as well as expression of their synthesizing enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX-2). Furthermore, PRE inhibited activity of mitogen-activated protein kinases (MAPK) as well as nuclear factor-kappa B (NF-${\kappa}B$) signaling pathway. Our findings are the first to explain the anti-inflammatory mechanism by PRE in LPS-stimulated macrophages. Given these results, we propose that PRE has therapeutic potential in the prevention of inflammatory disorders.

Genetic relationships and diversity within cultivated accessions of Salvia officinalis L. in Turkey

Tursun Ayse Ozlem,Sipahioglu Hikmet Murat,Telci Isa 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5

Sage (Salvia officinalis L.) is not found in Turkey's natural flora; however, its cultivation has been increasing in recent years. The species is widely cultivated in many provinces of the Aegean and the Mediterranean regions. There has been no informa- tion regarding genetic characteristics and the intra-individual phylogeny of cultivated sage. The levels of genetic relatedness of 19 Salvia samples from Izmir were determined by molecular tools. The sequences from the internal transcriber spacer (ITS) region of 18S–28S nuclear ribosomal DNA (nrDNA) locus were amplified by PCR and sequenced. Approximately, a 710 bp single amplified product was obtained in all genotypes. Of the nineteen Salvia specimens, six were identified as Salvia x sylvestris L. and the remaining 13 specimens were S. officinalis. The sequences of both species shared no similar secondary structures. The ITS region of all specimens contains several indels and substitutions. In the phylogenetic dendrogram, three major clusters (I, II, and III) and two independent branches (IV and V) were observed. Ninety-seven percent of local Salvia specimens were clustered in two branches (I and II), indicating a high diversity of genetic relatedness. Sequence divergence was higher among the specimens of S. officinalis than the specimens of Salvia x sylvestris.

Investigation of Thiol/Disulfide Balance in Obese Rats with Non-Alcoholic Fatty Liver Disease

Tursun, Serkan,Gulerman, Hacer Fulya,Gazyagci, Serkal,Sahin, Yasar,Erel, Ozcan,Neselioglu, Salim The Korean Society of Pediatric Gastroenterology 2021 Pediatric gastroenterology, hepatology & nutrition Vol.24 No.5

Purpose: Due to the increasing prevalence of obesity worldwide, non-alcoholic fatty liver disease (NAFLD) has reached epidemic dimensions over time. NAFLD is the most common cause of childhood chronic liver disease. There is a relationship between NAFLD and oxidative stress. This study aims to investigate the changes in thiol/disulfide homeostasis parameters to determine the oxidant/antioxidant balance in obese rats with diet-induced NAFLD and healthy rats. Methods: Twelve Wistar albino rats were used in this study. Experimentally produced NAFLD obese rats (n=6) and healthy rats were compared. Experimental NAFLD model was created with a special fatty liver diet (Altromin^® C1063, Fatty Liver Diet, Exclusivet, Lage, Germany). The biochemical and histopathological features of the groups, as well as serum thiol/disulfide homeostasis parameters, were analyzed and compared. Results: In the experimentally induced NAFLD rat model, they gained more weight than the control group. Steatosis (at least grade 2) occurred in all rats fed with special fatty liver diet for 12 weeks. Histopathologically, no high-grade inflammation was observed in rats with experimental NAFLD after feeding a diet for 12 weeks. Results revealed that aspartate transaminase and alanine transaminase levels were high, albumin levels were low, oxidant stress parameters increased, and antioxidant thiol groups decreased. Conclusion: Experimental NAFLD is characterized by increased oxidant stress accompanying fatty tissue in the liver. Analysis of thiol/disulfide homeostasis parameters in NAFLD can be used in further studies to develop effective treatment options.

The Synthesis of Magnetic Nano-Sized Iron Oxide from Liquid Solution by Adding Organic Components

Oybek, Tursun Kulov,Park, No Jin,Jo, Soo Jeong,Lee, Eun Young,Kamalov, Sharo Futdin,He, Li Li,Jeong, Soon Wook,Kim, Sung Jin Trans Tech Publications, Ltd. 2011 Materials science forum Vol.695 No.-

<P>Titanium silicon carbide (Ti3SiC2) is a remarkable material for its combination of the best properties of metals and ceramics. The high purity Ti3SiC2 ceramic has been prepared by infiltration sintering (IS), and the effect of a small amount of Si on Ti3SiC2 ceramic formation was investigated. The results show that the purity of Ti3SiC2 ceramic could be increased significantly and the sintering time for Ti3SiC2 could be decreased remarkably when proper amount of Si was added in the starting mixture. The Ti3SiC2 sintered compact with a purity of 99.2wt% and a relative density of 97% was obtained by the infiltration sintering from a starting mixture composed of n(Ti):n(Si):n(TiC) = 1:0.3:2 at 1500 °C with holding time of 2/3h.</P>

Anti-Inflammatory Effect of Rosa rugosa Flower Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

( Xirali Tursun ),( Yong Xin Zhao ),( Zulfiya Talat ),( Xue Lei Xin ),( Adilatursun ),( Rahima Abdulla ),( Haji Akberaisa ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Rosa rugosa Thunb, a deciduous shrub of the genus Rosa, has been widely used to treat stomach aches, diarrhoea, pain, and chronic inflammatory disease in eastern Asia. In recent years, our research team has extensively studied the Rosa rugosa flower extract, and specifically undertook pharmacological experiments which have optimized the extraction process. Our methods have yielded a standard extract enriched in phenolic compounds, named PRE. Herein, we expand our efforts and evaluated the antiinflammatory activity of PRE on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages. PRE significantly inhibited production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and interleukin 1β (IL-1β), as well as expression of their synthesizing enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX-2). Furthermore, PRE inhibited activity of mitogen-activated protein kinases (MAPK) as well as nuclear factor-kappa B (NF-κB) signaling pathway. Our findings are the first to explain the anti-inflammatory mechanism by PRE in LPS-stimulated macrophages. Given these results, we propose that PRE has therapeutic potential in the prevention of inflammatory disorders.

Anti-Inflammatory Effect of Rosa rugosa Flower Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Xirali Tursun,Yongxin Zhao,Zulfiya Talat,Xuelei Xin,AdilaTursun,Rahima Abdulla,Haji AkberAisa 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Rosa rugosa Thunb, a deciduous shrub of the genus Rosa, has been widely used to treat stomach aches, diarrhoea, pain, and chronic inflammatory disease in eastern Asia. In recent years, our research team has extensively studied the Rosa rugosa flower extract, and specifically undertook pharmacological experiments which have optimized the extraction process. Our methods have yielded a standard extract enriched in phenolic compounds, named PRE. Herein, we expand our efforts and evaluated the antiinflammatory activity of PRE on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages. PRE significantly inhibited production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and interleukin 1β (IL-1β), as well as expression of their synthesizing enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX-2). Furthermore, PRE inhibited activity of mitogen-activated protein kinases (MAPK) as well as nuclear factor-kappa B (NF-κB) signaling pathway. Our findings are the first to explain the anti-inflammatory mechanism by PRE in LPS-stimulated macrophages. Given these results, we propose that PRE has therapeutic potential in the prevention of inflammatory disorders.

Association analysis of polymorphisms in six keratin genes with wool traits in sheep

Sulayman, Ablat,Tursun, Mahira,Sulaiman, Yiming,Huang, Xixia,Tian, Kechuan,Tian, Yuezhen,Xu, Xinming,Fu, Xuefeng,Mamat, Amat,Tulafu, Hanikezi Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6

Objective: The purpose of this study was to investigate the genetic effects of six keratin (KRT) genes on the wool traits of 418 Chinese Merino (Xinjiang type) (CMXT) individuals. Methods: To explore the effects and association of six KRT genes on sheep wool traits, The polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP), DNA sequencing, and the gene pyramiding effect methods were used. Results: We report 20 mutation sites (single-nucleotide polymorphisms) within the six KRT genes, in which twelve induced silent mutations; five induced missense mutations and resulted in $Ile{\rightarrow}Thr$, $Glu{\rightarrow}Asp$, $Gly{\rightarrow}Ala$, $Ala{\rightarrow}Ser$, $Se{\rightarrow}His$; two were nonsense mutations and one was a same-sense mutation. Association analysis showed that two genotypes of the KRT31 gene were significantly associated with fiber diameter (p<0.05); three genotypes of the KRT36 gene were significantly associated with wool fineness score and fiber diameter (p<0.05), three genotypes of the KRT38 gene were significantly associated with the number of crimps (p<0.05); and three genotypes of the KRT85 gene were significantly associated with wool crimps score, body size, and fiber diameter (p<0.05). Analysis of the gene pyramiding effect between the different genotypes of the gene loci KRT36, KRT38, and KRT85, each genotype in a gene locus was combined with all the genotypes of another two gene loci and formed the different three loci combinations, indicated a total of 26 types of possible combined genotypes in the analyzed population. Compared with the other combined genotypes, the combinations CC-GG-II, CC-HH-IJ, CC-HH-JJ, DD-HH-JJ, CC-GH-IJ, and CC-GH-JJ at gene loci KRT36, KRT38, and KRT85, respectively, had a greater effect on wool traits (p<0.05). Conclusion: Our results indicate that the mutation loci of KRT31, KRT36, KRT38, and KRT85 genes, as well as the combinations at gene loci KRT36, KRT38, and KRT85 in CMXT have significant effects on wool traits, suggesting that these genes are important candidate genes for wool traits, which will contribute to sheep breeding and provide a molecular basis for improved wool quality in sheep.

Role of CXCR7 and Effects on CXCL12 in SiHa Cells and Upregulation in Cervical Squamous Cell Carcinomas in Uighur Women

Kurban, Shajidai,Tursun, Mikrban,Kurban, Gulinar,Hasim, Ayshamgul Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

CXCR7 is involved in tumor development and metastasis in multiple malignancies. However, the function and molecular mechanisms of action of CXCR7 in human cervical cancer are still unclear. In the present study a loss of-function approach was used to observe the effects of recombinant CXCR7 specific small interfering RNA pBSilence1.1 plasmids on biological behavior including proliferative activity and invasive potential, as indicated by MTT assays with the cervical cancer SiHa cell line in vitro. Reverse transcription polymerase chain reaction and Western blotting revealed that CXCR7 was downregulated in transfected compared with control cells, associated with inhibited cell growth, invasiveness and migration. The expression of CXCR7 and CXCL12 was also determined immunohistochemically in 152 paraffin-embedded, cervical squamous cell carcinoma (CSCC) and cervical intraepithelial neoplasia (CIN), or normal cervical epithelial to assess clinico-pathological pattern and CXCR7 status with respect to cell differentiation and lymph node metastasis in Uighur patients with CSCC. CXCR7 and CXCL12 expression was higher in cervical cancer than CIN and normal cervical mucosa, especially in those with higher stage and lymph node metastasis. CXCL12 appeared to be positively regulated by CXCR7 at the post-transcriptional level in CSCC. We propose that aberrant expression of CXCR7 plays a role in carcinogenesis, differentiation and metastasis of CSCC, implying its use as a potential target for clinical biomarkers in differentiation and lymph node metastasis.

Research on On-line Uyghur Handwritten Character Recognition Technology Based on Modified Center Distance Feature

Askar Hamdulla,Wujiahemaiti Simayi,Mayire Ibrayim,Dilmurat Tursun 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Through the analysis on the unique characteristics of Uyghur characters, in order to further improve the recognition rate, this paper developed the Center Distance Feature (CDF) to its modified form which is named as Modified Center Distance Feature (MCDF). By combination with some low dimensional features including stroke number feature, additional part’s location feature, shape feature, bottom-up and left-right density feature(BULR) in experiments, MCDF gifted robust recognition accuracy of 98.77% for the 32 isolated forms of Uyghur characters. MCDF increased the recognition accuracy by 4.51 points comparing with the result from the combination of CDF with the same low dimensional features mentioned above, which is 94.16%. This paper used the samples from 400 different volunteers. The recognition system is trained using 70 percent of 12800 samples from 400 different writers and tested on the remained 30 percent.

Survey on the Features for Recognition of on-line Handwritten Uyghur Characters

Wujiahemaiti Simayi,Mayire Ibrayim,Dilmurat Tursun,Askar Hamdulla 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.9

This survey attempts to provide a detailed overview of the previous work on Uyghur on-line handwritten character recognition technology and mainly focuses on investigating the feature extraction algorithms that have been presented or applied to Uyghur characters. Statistical, structural and combined-hybrid approaches for feature extraction technology for Uyghur characters are going to be explored. Besides the popular feature extraction methods which perhaps are granted to be taken into consideration for all letter kinds, the study on Uyghur handwritten recognition contributed new features and extraction algorithms for pattern recognition. Summarization on observing methods and extracted features from the published work and suggestions for further attempts would be valuable reference for the up-coming research activities on finding more efficient features.