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Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
Factors Affecting Tourists’ Satisfaction in Associated Tourism Chains
Ha NGUYEN VAN,Thi Kim Hao DO,Thi Viet Ha NGUYEN,Ngoc Tien NGUYEN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.6
Tourism is an interdisciplinary, inter-regional, inter-territory integrated economy; therefore, the construction of an associated tourism chain will help localities in the region take greater advantage of tourism resources and tourism products to meet the diverse needs of visitors (UNWTO, 2013). This research seeks to identify the factors affecting the satisfaction of tourists in the associated tourism chain in four central provinces of Vietnam – Binh Dinh, Phu Yen, Gia Lai, and Dak Lak. The study surveyed 1,361 tourists who have participated in an affiliated tour chain in these provinces during the March–April 2020 period. The survey was conducted online with the help of Google Form and the data collected were processed using descriptive statistics, exploratory factor analysis, and regression analysis. The research results show that two main factors are affecting the satisfaction of tourists in the associated tourism chain in the above regions, namely, (i) tourism resources (cultural beauty in localities in the associated tourism chain, local cuisine in the affiliated travel chain, natural landscapes/local attractions in the linked tourism chain, etc.); and (ii) tourism environment (the climate in localities in the associated tourism chain, the clean and fresh environment in localities in the associated tourism chain, etc.).
Ha Thi Thanh Tran,Duc Anh Truong,Viet Duc Ly,Hao Thi Vu,Tuan Van Hoang,Chinh Thi Nguyen,Nhu Thi Chu,Vinh The Nguyen,Duyen Thuy Nguyen,Kohtaroh Miyazawa,Takehiro Kokuho,Hoang Vu Dang 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.1
Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.
Nguyen Thi Duyen,Nguyen Minh Khoi,Phan Nguyen Truong Thang,Duong Minh Tan,Tran Viet Hung,Do Thi Ha 한국생약학회 2020 Natural Product Sciences Vol.26 No.4
A new compound, 3β-acetoxylanosta-7,9(11),24-triene-26-al (3), and seven known compounds (1 – 2 and 4 – 8) were isolated from Ganoderma tropicum (Jung.) Bres. collected in Tay Nguyen, Vietnam. The structures of these compounds were determined by one- and two-dimensional nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and high-resolution ESI-MS, and by comparison with literature data. All of the isolated compounds were tested for nitroblue tetrazolium (NBT) reduction activity in Saccharomyces cerevisiae-stimulated RAW 246.7 cells. Among them, compounds 2 – 4 and 6 – 8 enhanced the NBT reduction in a dose-dependent manner.
Van-Truc Nguyen,Thanh-Binh Nguyen,Thi-Dieu-Hien Vo,Nguyen Duy Dat,Thi-Kim Quyen Vo,Xuan Cuong Nguyen,Viet-Cuong Dinh,Thi-Ngoc-Chau Le,Thi-Giang-Huong Duong,Manh-Ha Bui,Xuan-Thanh Bui 대한환경공학회 2023 Environmental Engineering Research Vol.29 No.3
This study explores the adsorption of doxycycline (DOX) from aqueous solutions onto biochar derived from banana peel, which was prepared using a potassium hydroxide activation method (KOH-BPB). The biochar properties were characterized based on morphology, surface area (SBET of 710.241 ㎡ g<SUP>−1</SUP>), functional groups, and surface charge (pHPZC = 7.7). Parameters, including initial pH, DOX concentration, and ionic strength, that influenced the DOX adsorption capacity of KOH-BPB were examined. Adsorption equilibrium of DOX on KOH-BPB was assessed through four isothermal models: the Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich models. The obtained data were most compatible with the Langmuir model (R² = 0.9879). KOH-BPB has a maximum DOX absorption capacity of 121.95 mg g<SUP>-1</SUP> which exceeds that of many comparable absorbents. The maximum DOX removal was 96.7% at pH 6, a DOX concentration of 20 mg L<SUP>-1</SUP>, and a KOH-BPB dose of 1.0g L<SUP>-1</SUP>. These findings reveal that biochar from banana peel effectively removes antibiotic residues from water. This study provides a potential, low-cost, and environmentally friendly adsorbent.
( Hai The Pham ),( Phuong Ha Vu ),( Thuy Thu Thi Nguyen ),( Ha Viet Thi Bui ),( Huyen Thanh Thi Tran ),( Hanh My Tran ),( Huy Quang Nguyen ),( Byung Hong Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.10
Sediment bioelectrochemical systems (SBESs) can be integrated into brackish aquaculture ponds for in-situ bioremediation of the pond water and sediment. Such an in-situ system offers advantages including reduced treatment cost, reusability and simple handling. In order to realize such an application potential of the SBES, in this laboratory-scale study we investigated the effect of several controllable and uncontrollable operational factors on the in-situ bioremediation performance of a tank model of a brackish aquaculture pond, into which a SBES was integrated, in comparison with a natural degradation control model. The performance was evaluated in terms of electricity generation by the SBES, Chemical oxygen demand (COD) removal and nitrogen removal of both the tank water and the tank sediment. Real-life conditions of the operational parameters were also experimented to understand the most close-to-practice responses of the system to their changes. Predictable effects of controllable parameters including external resistance and electrode spacing, similar to those reported previously for the BESs, were shown by the results but exceptions were observed. Accordingly, while increasing the electrode spacing reduced the current densities but generally improved COD and nitrogen removal, increasing the external resistance could result in decreased COD removal but also increased nitrogen removal and decreased current densities. However, maximum electricity generation and COD removal efficiency difference of the SBES (versus the control) could be reached with an external resistance of 100 Ω, not with the lowest one of 10 Ω. The effects of uncontrollable parameters such as ambient temperature, salinity and pH of the pond (tank) water were rather unpredictable. Temperatures higher than 35℃ seemed to have more accelaration effect on natural degradation than on bioelectrochemical processes. Changing salinity seriously changed the electricity generation but did not clearly affect the bioremediation performance of the SBES, although at 2.5% salinity the SBES displayed a significantly more efficient removal of nitrogen in the water, compared to the control. Variation of pH to practically extreme levels (5.5 and 8.8) led to increased electricity generations but poorer performances of the SBES (vs. the control) in removing COD and nitrogen. Altogether, the results suggest some distinct responses of the SBES under brackish conditions and imply that COD removal and nitrogen removal in the system are not completely linked to bioelectrochemical processes but electrochemically enriched bacteria can still perform nonbioelectrochemical COD and nitrogen removals more efficiently than natural ones. The results confirm the application potential of the SBES in brackish aquaculture bioremediation and help propose efficient practices to warrant the success of such application in real-life scenarios.
Manh Hung Do,비엣 흥 응웬(Viet Hung Nguyen),투하 응웬 티(Thu Ha Nguyen Thi),한 호앙 티(Hanh Hoang Thi),프엉 투 응웬 티(Phuong Thu Nguyen Thi) 영남대학교 박정희새마을연구원 2018 새마을학연구 Vol.3 No.2
베트남의 1986년도 정치경제 개혁, 즉 도어머이(Doi Moi)는 베트남의 경제를 개방적 사회주의경제로 전환시켰으며, 이를 계기로 베트남 경제는 획기적으로 근대화되었다. 그러나 이 과정에서 급속한 도시화는 도농 간 불균형을 가져왔으며, 이 도농 간 격차를 줄이기 위해 베트남 정부는 2006년에 새농촌개발(NRD) 프로그램을 도입하기에 이르렀다. 이 프로그램은 농촌 지역의 사회기반시설의 확충과 생활환경의 개선, 생산 활동의 종합적 발전, 소득증대, 농민들의 육체적ㆍ정신적 건강 증진 등을 목표로 하고 있었다. 이 논문의 주된 목적은 NRD 프로그램의 시행이 NRD의 궁극적 목적 중 하나인 농가소득 증대에 미치는 영향에 대한 과학적 증거를 제시하는 데 있다. 또한 이와 더불어 새마을운동의 시범사업을 실시한 지역과 그렇지 않은 지역 간에 소득 증대에서 차이가 있는가를 다중회귀분석을 통해 밝혀보고자 한다. 분석 결과는 NRD의 시행이 상당히 긍정적 결과를 가져왔으며 코민 수준에서 농가소득 증대와 생활환경의 개선에 중요한 영향을 미쳤다는 것을 보여주었다. 또한 도로 건설, 전기 도입, 주민 역량 강화, 제도의 발전 등이 농가소득 증대에 큰 영향을 미쳤음을 확인했다. 회귀분석의 결과는 또한 새마을 사업을 시행하고 있는 코뮌들이 그렇지 않은 코뮌에 비해 연소득이 1200만 동(미화530달러) 더 많다(또는 41.8% 더 높다)는 것을 보여주었다. The economic and political reform of Vietnam in 1986 (the Doi Moi) has transformed its economy positively and significantly into an open socialist-oriented market economy. This caused a dramatic change of Vietnam’s economy towards modernization. However, the urbanization process created an unbalanced development between the urban and rural regions. To solve the gaps between these regions, the government of Vietnam firstly introduced the New Rural Development (NRD) program in 2006 to encourage and mobilize a revolutionary and campaign to improve local infrastructure, living condition, comprehensive development of related-production activities in rural regions, as well as increase income, and improve physical and mental health of people living in rural communities. One of the main purposes of this research paper is aimed at providing a scientific evidence for justifying whether the NRD implementation could have an impact on rural households’ income, one of the ultimate goals of the NRD, and examining the difference of incomes between Saemaul Undong (SU)-piloted provinces with the other provinces by employing a multiple regression model. The analysis results show that the NRD implementation has shown positive results and significant impacts on increasing rural households’ income and improving living conditions at the communal levels. Criteria such as road development, access to electricity, capacity building, and institutional development have significantly influenced on the households’ income. The regression model also indicates that the communes currently implementing SU pilots have a better annual income of 12 million VND (~US$ 530) or 41.8% higher than the other communes.