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Linh Thi Thao Nguyen,송연우,Thao Anh Tran,김기석,김소미 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4
Anoikis (or cell-detachment-induced apoptosis) is aspecial form of apoptosis in which cells loose contact with othercells or the extracellular matrix. In the present study, we havefound that the breast cancer stem cell line MCF-7-SC, isolatedfrom MCF-7 breast cancer cells, shows characteristics of anoikisresistance. Treatment of supercritical CO2 extract from Citrushassaku Hort ex Tanaka, locally known as Phalsak, reduced theexpression of ATP-binding cassette sub-family G member 2mRNA and induced apoptosis in MCF-7-SCs, as evidenced by anincrease of apoptotic body formation, increased cell population inthe sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolyticactivation of caspase-9 and caspase-3, and the degradation ofpoly(ADP-ribose) polymerase (PARP) protein. The effects ofsupercritical CO2 extract from Phalsak on anoikis-resistant breastcancer stem cells suggest the use of this extract as a potentialtherapeutic agent for breast cancer stem cell treatment.
Nguyen, Linh Thi Thao,Song, Yeon Woo,Tran, Thao Anh,Kim, Ki-Seok,Cho, Somi Kim The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4
Anoikis (or cell-detachment-induced apoptosis) is a special form of apoptosis in which cells loose contact with other cells or the extracellular matrix. In the present study, we have found that the breast cancer stem cell line MCF-7-SC, isolated from MCF-7 breast cancer cells, shows characteristics of anoikis resistance. Treatment of supercritical $CO_2$ extract from Citrus hassaku Hort ex Tanaka, locally known as Phalsak, reduced the expression of ATP-binding cassette sub-family G member 2 mRNA and induced apoptosis in MCF-7-SCs, as evidenced by an increase of apoptotic body formation, increased cell population in the sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolytic activation of caspase-9 and caspase-3, and the degradation of poly(ADP-ribose) polymerase (PARP) protein. The effects of supercritical $CO_2$ extract from Phalsak on anoikis-resistant breast cancer stem cells suggest the use of this extract as a potential therapeutic agent for breast cancer stem cell treatment.
Thao, Le Quang,Lee, Changkyu,Kim, Bomi,Lee, Sungin,Kim, Tae Hwan,Kim, Jong Oh,Lee, Eun Seong,Oh, Kyung Taek,Choi, Han-Gon,Yoo, Sun Dong,Youn, Yu Seok Elsevier 2017 Colloids and surfaces Biointerfaces Vol.152 No.-
<P><B>Abstract</B></P> <P>Anticancer drug targeting to liver asialoglycoprotein receptors (ASGPR) is viewed as a good approach for hepatocellular carcinoma (HCC) treatment. Lactose residue is a promising ASGPR ligand due to its high receptor affinity. Herein, we introduce doxorubicin and paclitaxel co-bound lactosylated albumin (Lac-BSA) nanoparticles (Dox/Pac Lac-BSA NPs) with good liver targetability. Lac-BSA was synthesized by conjugating lactobionic acid to naïve BSA then characterized by mass spectrometry. Dox/Pac Lac-BSA NPs were fabricated utilizing high-pressure homogenization and evaporation with Nab<SUP>®</SUP> (nanoparticle albumin bound) technology. Dox/Pac Lac-BSA NPs were spherical and well-dispersed, with a 148.7±13.8nm particle size and −54.1±0.7mV zeta potential at a 100% Lac-BSA feed ratio. Combined Dox and Pac synergistic cytotoxicity was confirmed in Hep G2 cells. Specifically, the inhibitory concentration (IC<SUB>50</SUB>; 0.21±0.02μg/ml) for Dox/Pac Lac-BSA NPs was 3.2 time lower than plain Dox/Pac BSA NPs (IC<SUB>50</SUB>; 0.68±0.04μg/ml). Also, Dox/Pac Lac-BSA NPs exhibited better internalizing in Hep G2 cells (61.8% vs. 14.4% for Dox) and spheroids compared to Dox/Pac BSA NPs. Finally, Dox/Pac Lac-BSA NPs displayed much greater localization into ICR mice livers compared to Dox/Pac BSA NPs. This was indicated by the presence of NP lactose residues revealed by a galactose inhibition study. Based on these results, we suggest that lactose-modified albumin-based nanoparticles fabricated with the Nab<SUP>®</SUP> technique can be a potential therapeutic vector for treating HCC via hepatocyte targeting.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Thao Hong Phuong CHAU,Truc Dinh LE,Quynh Xuan PHAM 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.8
The purpose of this research is to investigate the mediating role of performance information use in the relationship between result-oriented culture and organizational performance. The data were collected from chief accountants and managers working in public-sector organizations in Vietnam. The analysis was based on the 197 completed questionnaires by using Partial Least Squares Structural Equation Modeling (PLSSEM). For the test research model and hypotheses, the supporting tool is SmartPLS 3 software. Research findings show that result-oriented culture is positively associated with organizational performance, and that performance information use is a mediating mechanism that explains how a result-oriented culture leads to higher performance in public organizations. The findings of this study suggest that the resultsoriented culture, not only directly improves organizational performance, but also indirectly does so by the use of performance information. This study provides evidence for theoretical understanding of the impact of result-oriented culture on the performance of public-sector organizations through performance information use, and suggests practical implications for public organizations in applying result-oriented culture to foster using performance measurement and improve organizational performance. In order to achieve good performance, publicsector organizations need to create and spread result-oriented culture and enhance the use of performance information for decision-making.
Thao Tram Le Nguyen,Xuan Loc Vu Doan,Vy Thuc Nguyen 아시아심장혈관영상의학회 2020 Cardiovascular Imaging Asia Vol.4 No.4
Anomalous left coronary artery from the pulmonary artery (ALCAPA) syndrome is a rare congenital coronary artery anomaly. Clinical presentation can range from asymptomatic conditions to sudden death with exertion. The majority of cases is diagnosed in childhood and dies within the first year, however medical literature has confirmed several cases in adults. We report a case of a 49-year-old woman hospitalized for vague chest pain. Echocardiography found collateral circulation on the anterior wall, and ALCAPA syndrome was diagnosed on multislice CT.
DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells
Thao Dang,장수화,백성훈,박정우,한인섭 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.12
The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased ‘slow and long-lived’ subpopulations, but decreased ‘fast and short-lived’ subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells.
Thao, N.T.,Wanapat, M.,Kang, S.,Cherdthong, A. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.7
Four rumen fistulated swamp buffaloes were randomly assigned according to a $4{\times}4$ Latin square design to investigate the effects of Eucalyptus (E. Camaldulensis) leaf meal (ELM) supplementation as a rumen enhancer on feed intake and rumen fermentation characteristics. The dietary treatments were as follows: T1 = 0 g ELM/hd/d; T2 = 40 g ELM/hd/d; T3 = 80 g ELM/hd/d; T4 = 120 g ELM/hd/d, respectively. Experimental animals were kept in individual pens and concentrate was offered at 0.3% BW while rice straw was fed ad libitum. The results revealed that voluntary feed intake and digestion coefficients of nutrients were similar among treatments. Ruminal pH, temperature and blood urea nitrogen concentrations were not affected by ELM supplementation; however, ELM supplementation resulted in lower concentration of ruminal ammonia nitrogen. Total volatile fatty acids, propionate concentration increased with the increasing level of EML (p<0.05) while the proportion of acetate was decreased (p<0.05). Methane production was linearly decreased (p<0.05) with the increasing level of ELM supplementation. Protozoa count and proteolytic bacteria population were reduced (p<0.05) while fungal zoospores and total viable bacteria, amylolytic, cellulolytic bacteria were unchanged. In addition, nitrogen utilization and microbial protein synthesis tended to increase by the dietary treatments. Based on the present findings, it is suggested that ELM could modify the rumen fermentation and is potentially used as a rumen enhancer in methane mitigation and rumen fermentation efficiency.