Effects of soil-treatment with fungal biopesticides on pupation behaviors, emergence success and fitness of tea geometrid, Ectropis grisescens (Lepidoptera: Geometridae)

TaoMa,Huifang Wang,Shiping Liang,Qiang Xiao,Panrong Cao,Xuan Chen,Ying Niu,Yurong He,Zhaohui Sun,XiujunWen,Cai Wang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Ectropisgrisescens Warren (Lepidoptera: Geometridae) is one of the most severe defoliating pests of tea plants. Synthetic pesticides have been widely applied to control this pest in tea plantations, but pesticide residues may decrease the quality and safety of tea products. In the present study, we hypothesized that soil treatment with the two commercial biopesticides (Shuiguxin ® ) based on Metarhizium anisopliae (Metchnikoff) Sorokin and Beauveria bassiana (Balsamo) could reduce the survivorship and fitness of E. grisescens. Wandering larvae of E. grisescens were allowed to pupate in soil treated with each biopesticide, and the concentrations of M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) that produced the 50% mortality values (LC 50 ) were 2.9 × 10 6 and 1.6 × 10 7 conidia/g soil, respectively. Artificial burying the pupae using soil treated with M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) (1 × 10 8 or 1 × 10 9 conidia/g soil for both biopesticides) also significantly reduced emergence success of E. grisescens. In addition, choice tests showed that soil treated with the high concentration of M. anisopliae (Shuiguxin ® ) or B. bassiana (Shuiguxin ® ) had repellent effects on pupating E. grisescens. However, sublethal concentrations (LC 25 and LC 50 ) of both biopesticides did not significantly affect fecundity, fertility and longevity of post-emerged adults. Our study showed that soil treatment with the two commercial biopesticides caused direct mortality of pupating E. grisescens, but may not effectively suppress E. grisescens populations at sublethal concentrations. The realistic application of the fungal dosage in fields should be determined in future studies

Characteristics of Desulfurized Rubber Asphalt and Mixture

Tao Ma,Yongli Zhao,Xiaoming Huang,Yao Zhang 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4

This paper investigated the modification mechanism of the desulfurized rubber asphalt, and compared its performances with those of crumb rubber asphalt. The microstructures of desulfurized rubber asphalt and crumb rubber asphalt were identified by using the scanning electron microscope technology, component analysis and infrared spectrum analysis. Conventional and Strategic Highway Research Program (SHRP) performance tests were conducted to evaluate the performances of desulfurized rubber asphalt and crumb rubber asphalt. Mixture performance tests were also performed to characterize the high temperature rutting resistance, low temperature cracking resistance, moisture stability and fatigue life of the desulfurized rubber asphalt mixture and crumb rubber asphalt mixture. It was found that the modification mechanisms of desulfurized rubber asphalt and crumb rubber asphalt are different. The desulfurized rubber can integrate into the asphalt while the crumb rubber mainly exists incompatibly in asphalt. This difference results in the various performances between the desulfurized rubber asphalt and crumb rubber asphalt. Both crumb rubber and desulfurized rubber can improve the performance of asphalt binder and mixture, but the desulfurized rubber asphalt has much lower viscosity and better storage stability than the crumb rubber asphalt. The preparing temperature of the desulfurized rubber asphalt mixture can be reduced by 10~20°C compared to the crumb rubber asphalt mixture. Moreover, without the interference of solid rubber particles, the desulfurized rubber asphalt can be used in dense-graded asphalt mixture while crumb rubber asphalt mixture tends to use gap gradation with high asphalt content.

Prediction of methane emission from sheep based on data measured in vivo from open-circuit respiratory studies

Tao Ma,Kaidong Deng,Qiyu Diao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.9

Objective: The current study analysed the relationships between methane (CH4) output from animal and dietary factors. Methods: The dataset was obtained from 159 Dorper×thin-tailed Han lambs from our seven studies, and CH4 production and energy metabolism data were measured in vivo by an open-circuit respiratory method. All lambs were confined indoors and fed pelleted diet during the whole experimental period in all studies. Data from two-thirds of lambs were used to develop linear and multiple regressions to describe the relationship between CH4 emission and dietary variables, and data from the remaining one third of lambs were used to validate the established models. Results: CH4 emission (g/d) was positively related to dry matter intake (DMI) and gross energy intake (GEI) (p<0.001). CH4 energy/GEI was negatively related to metabolizable energy/gross energy and metabolizable energy/digestible energy (p<0.001). Using DMI to predict CH4 emission (g/d) resulted in a coefficient of determination (R2) of 0.80. Using GEI, digestible energy intake, and metabolizable energy intake predict CH4 energy/GEI resulted in a R2 of 0.92. Conclusion: the prediction equations established in the current study are useful to develop appropriate feeding and management strategies to mitigate CH4 emissions from sheep.

A Novel In Situ Gel Formulation of Ranitidine for Oral Sustained Delivery

( Tao Ma ),( Hao Ping Xu ),( Min Shi ),( Jin Ling Jiang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2

The main purpose of this study was to develop a novel, in situ gel system for sustained delivery of ranitidine hydrochloride. Ranitidinein situ gels at 0.2%, 0.5%, and 1.0% gellan gum concentration (w/v) were prepared, respectively, and characterized in termsof preparation, viscosity and in vitro release. The viscosity of the gellan gum formulations in solution increased with increasingconcentrations of gellan gum. In vitro study showed that the release of ranitidine from these gels was characterized by an initialphase of high release (burst effect) and translated to the second phase of moderate release. Single photon emission computingtomography technique was used to evaluate the stomach residence time of gel containing 99mTc tracer. The animal experimentsuggested in situ gel had feasibility of forming gels in stomach and sustained the ranitidine release from the gels over the periodof at least 8 h. In conclusion, the in situ gel system is a promising approach for the oral delivery of ranitidine for the therapeuticeffects improvement.

No-substrate and low-moisture conditions during pupating adversely affect Ectropis grisescens (Lepidoptera: Geometridae) adults

Huifang Wang,Shiping Liang,TaoMa,Qiang Xiao,Panrong Cao,Xuan Chen,Wenquan Qin,Hongpeng Xiong,Zhaohui Sun,XiujunWen,Cai Wang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Ectropis grisescens Warren (Lepidoptera: Geometridae) is an important defoliator pest of the tea plant, Camellia sinensis L. Our previous studies showed that E. grisescens pupae had significantly lower body water content when they pupated in the dry soil (20%-moisture) than those that pupated in the wetter soil (50%- or 80%-moisture). However, it is still unclear whether low-moisture conditions during pupating would further influence the physiological and fitness traits of adults. In the present study, E. grisescens individuals were allowed to pupate in 20%-, 50%- or 80%-moisture soil (silt loam), or in containers without soil (no-substrate conditions) in the laboratory. No significant difference in emergence was detected among different pupating conditions. However, female moths that emerged from 20%-moisture soil had significantly lower body water content compared to 80%-moisture soil, whereas the body water content of males was similar among the four treatments. The paired moths that emerged from 20%-moisture soil laid significantly fewer eggs than those that emerged from 50%- moisture soil. In addition, the number of hatched offerings (larvae) were significantly less when the adults previously pupated in no-soil containers compared to 50%-moisture soil. The longevity of adults was shortest when they emerged from 20%-moisture soil. Our study showed that the no-substrate and low-moisture conditions during pupating adversely affected E. grisescens adults.

Laboratory and field evaluation of the repellency of six preservatives to red imported fire ants (Hymenoptera: Formicidae)

Wenquan Qin,Hongpeng Xiong,YuzhenWen,XiujunWen,HuiWang,Yihan Fang,TaoMa,Zhaohui Sun,Xiaoyang Chen,Cai Wang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Baiting is widely applied for the detection and control of the red imported fire ants, Solenopsis invicta Buren. Adding preservatives into baits would prevent microbial contamination during production and storage, and might extend period of bait attractiveness in the field. However, it is not known if preservatives are repellent to S. invicta. In the present study, potential effects of six commonly used preservatives (potassiumsorbate, sodium benzoate, sodium diacetate, sodium dehydroacetate, calcium propionate, and nisin) on the foraging behaviors of S. invicta foragers were evaluated under laboratory and field conditions. Four-choice tests (laboratory study) showed that the number of foraging ants on the preservative-treated (immersed in 2000-, 5000-, or 10,000-ppm solution for 30 min) and control (immersed in distilled water for 30 min) food was similar, with an exception of sodium dehydroacetate, which significantly decreased the number of foraging ants at the three tested concentrations. Interestingly, 46%, 58% and 71% of the food items treated with 2000-, 5000- and 10,000- ppm solutions of sodium dehydroacetate were buried with soil particles, respectively, whereas only 17% of the control food was similarly buried. Multiple-choice tests (field study) showed that food treated with the 5000- or 10,000-ppm solution of sodium dehydroacetate attracted significantly fewer S. invicta workers compared to other preservatives and the controls. Our study showed that the use of sodiumdehydroacetate should be avoided during the production of aqueous-based or liquid baits against S. invicta.