Anti-Angiogenic Activity of Gecko Aqueous Extracts and its Macromolecular Components in CAM and HUVE-12 Cells

Tang, Zhen,Huang, Shu-Qiong,Liu, Jian-Ting,Jiang, Gui-Xiang,Wang, Chun-Mei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Gecko is a kind of traditional Chinese medicine with remarkable antineoplastic activity. However, undefined mechanisms and ambiguity regarding active ingredients limit new drug development from gecko. This study was conducted to assess anti-angiogenic properties of the aqueous extracts of fresh gecko (AG) or macromolecular components separated from AG (M-AG). An enzyme-linked immunosorbent assay (ELISA) approach was applied to detect the vascular endothelial growth factor (VEGF) secretion of the tumor cells treated with AG or M-AG. The effect of AG or M-AG on vascular endothelial cell proliferation and migratory ability was analyzed by tetrazolium dye colorimetric method, transwell and wound-healing assays. Chick embryo chorioallantoic membrane (CAM) assays were used to ensure the anti-angiogenic activity of M-AG in vivo. The results showed that AG or M-AG inhibited the VEGF secretion of tumor cells, the relative inhibition rates of AG and M-AG being 27.2% and 53.2% respectively at a concentration of $20{\mu}L/mL$. AG and M-AG inhibited the vascular endothelial (VE) cell proliferation with IC50 values of $11.5{\pm}0.5{\mu}L/mL$ and $12.9{\pm}0.4{\mu}L/mL$ respectively. The VE cell migration potential was inhibited significantly (p<0.01) by the AG (${\geq}24{\mu}L/mL$) or M-AG (${\geq}12\mu}L/mL$) treatment. In vivo, neovascularization of CAM treated with M-AG was inhibited significantly (p<0.05) at a concentration of ${\geq}0.4{\mu}L/mL$. This study provided evidence that anti-angiogenesis is one of the anti-tumor mechanisms of AG and M-AG, with the latter as a promising active component.

Insulin receptors regulate the fecundity of Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Liu Yong-Kang,Luo Yu-Jia,Deng Ying-Mei,Li Yan,Pang Xiao-Qing,Xu Cai-Di,Wang Shi-Gui,Tang Bin 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Two InR (insulin receptor) genes have been identified in the Nilaparvata lugens. In this study, we used RNA interference (RNAi) to investigate the role of InR genes in the fecundity of N. lugens. The expression of NLInR1 and NLInR2 genes was simultaneously silenced with mixture of dsInR1 and dsInR2 (dsInRs) injection. Our results showed that larvae RNAi against both NLInR1 and NLInR2 reduced the number of eggs laid by N. lugens and some eggs as well as ovaries were abnormal. In addition, the relative expression of Vg (vitellogenin) and VgR (vitellogenin receptor) was significantly reduced on the 4th and 6th days after insects treated with larvae RNAi reached the adult stage. We also determined the relative expression levels of insulin/insulin-like signaling (IIS) related genes in RNAi-treated larvae and found that the expression levels of Chico (homologous receptor substrate), Akt (protein kinase B), PI3K (phosphoinositide 3-kinase), and PTEN (phosphatase and tensin homolog) genes decreased whereas FOXO (forkhead box O) and GSK3 (glycogen synthase kinase-3) levels increased on the 4th and 6th days after insects reached the adult stage. These results indicate that silencing of NLInR1 and NLInR2 reduces the fecundity of N. lugens through the IIS pathway.

Cervical Cancer Gene Therapy by Gene Loaded PEG-PLA Nanomedicine

Liu, Bo,Han, Shu-Mei,Tang, Xiao-Yong,Han, Li,Li, Chang-Zhong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background and Aims: Advances in the treatment of cervical cancer over the last decade have predominantly involved the development of genes directed at molecular targets. Gene therapy is recognized to be a novel method for the treatment of cervical cancer. Genes can be administered into target cells via nanocarriers. This study aimed to develop systemically administrable nano-vectors. Floate (Fa) containing gene loaded nanoparticles (NPs) could target HeLa human cervical cancer cells through combination with receptors on the cells to increase the nuclear uptake of genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly (D, L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro transfection efficiency of Fa-NPs/DNA was evaluated in HeLa cells and human umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183 nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher than naked DNA in HeLa cells. However, in HUVECs, no significant difference appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function as excellent materials for gene loading. This nano-approach could be used as tumor cell targeted medicine for the treatment of cervical cancer.

Adaptive Consensualization for Lipschitz Nonlinear-type Multi-agent Networks with Fixed Topologies

Tang Zheng,Jianxiang Xi,Mei Yuan,Guangbin Liu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.4

Adaptive consensualization problems for Lipschitz nonlinear-type multi-agent networks with fixed topologies are addressed. By using the state errors of the neighboring agents, an adaptive consensus protocol is proposed to deal with the consensualization problems. To eliminate the impacts of the Lipschitz nonlinear dynamics, the structure property of a transformation matrix and the Laplacian matrix, and the Lipschitz condition are well utilized. In the Lyapunov function, a scaling constant is introduced to eliminate the impacts of eigenvalues of the Laplacian matrix, which are the global information of the whole multi-agent networks. Then, an completely distributed consensualization criterion is presented in terms of the linear matrix inequality. Furthermore, an explicit expression of the consensus function and its initial state of this multi-agent network are presented. In the Final, a numerical example is shown to verify the validity of theoretical results.

Heart Rate Variability Biofeedback Increased Autonomic Activation and Improved Symptoms of Depression and Insomnia among Patients with Major Depression Disorder

I-Mei Lin,Sheng-Yu Fan,Cheng-Fang Yen,Yi-Chun Yeh,Tze‐Chun Tang,Mei-Feng Huang,Tai-Ling Liu,Peng-Wei Wang,Huang-Chi Lin,Hsin-Yi Tsai,Yu-Che Tsai 대한정신약물학회 2019 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.17 No.2

Objective: Autonomic imbalance is considered a psychopathological mechanism underlying major depressive disorder (MDD). Heart rate variability (HRV) is an index for autonomic activation. Poor sleep quality is common among patients with MDD. HRV biofeedback (BF) has been used for regulating autonomic balance among patients with physical illness and mental disorders. The purpose of present study was to examine the effects of HRV-BF on depressive symptoms, sleep quality, pre-sleep arousal, and HRV indices, in patients with MDD and insomnia. Methods: In this case-controlled study, patients with MDD and Pittsburgh Sleep Quality Index (PSQI) score higher than 6 were recruited. The HRV-BF group received weekly 60-minute protocol for 6 weeks, and the control group who have matched the age and sex received medical care only. All participants were assessed on Beck Depression Inventory-II, Back Anxiety Inventory, PSQI, and Pre-Sleep Arousal Scale. Breathing rates and electrocardiography were also performed under resting state at pre-testing, and post-testing conditions and for the HRV-BF group, also at 1-month follow-up. Results: In the HRV-BF group, symptoms of depression and anxiety, sleep quality, and pre-sleep arousal were significantly improved, and increased HRV indices, compared with the control group. Moreover, in the HRV-BF group, significantly improved symptoms of depression and anxiety, decreased breathing rates, and increased HRV indices were detected at post-testing and at 1-month follow-up, compared with pre-testing values. Conclusion: This study confirmed that HRV-BF is a useful psychosocial intervention for improving autonomic balance, baroreflex, and symptoms of depression and insomnia in MDD patients.

Geochronological and geochemical constraints on the ore-related granites in Huanggang deposit, Southern Great Xing’an Range, NE China and its tectonic significance

Wei Mei,Xinbiao Lü,Zhi Liu,Rankun Tang,Zhilong Ai,Xiangdong Wang,Mamady Cisse 한국지질과학협의회 2015 Geosciences Journal Vol.19 No.1

Huanggang skarn Fe-Sn deposit is located in themain ridge Sn-polymetallic metallogenic sub-belt of the SouthernGreat Xing’an Range. A combination of zircon U-Pb age, geochemistryand Hf isotopic composition are reported for the orerelatedgranite (K-feldspar granite) of the Huanggang deposit toconstrain its petrogenesis and tectonic setting. LA-ICP-MS U-PbZircon dating results indicate that the K-feldspar granite wasintruded at 145.3 ± 1.6Ma. The K-feldspar granites belong to high-K calc-alkaline and shoshonite series, mainly peraluminous. Theserocks have a negative slope from La to Lu with strongly negativeEu anomalies, they demonstrate negative Ba, Sr P, Ti and Nbspikes and positive Th, U, Nd spikes on the normalized incompatibleelement diagrams. The regional geological setting togetherwith the geochemistry of the K-feldspar granite indicates that it isformed in a post-orogenic setting. The zircon Hf isotopic compositions(εHf(t): +5.2 to +6.6, 176Hf/177Hf: 0.282837‒0.282876) indicateboth juvenile crust sources that originated from depleted mantleand some ancient continental crust sources contributed to its origin,and there is a significant addition of juvenile crust under theSouthern Great Xing’an Range during the time from Paleo-Neoproterozoicto Phanerozoic.

New Triterpenoids from the Fruits of Schisandra wilsoniana and Their Biological Activities

Gao, Xue-Mei,Li, Yun-Qi,Shu, Li-Dan,Shen, Yan-Qiong,Yang, Li-Ying,Yang, Liu-Meng,Zheng, Yong-Tang,Sun, Han-Dong,Xiao, Wei-Lie,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3

Investigation of an organic extract of the fruits Schisandra wilsoniana led to the isolation of two new highly oxygenated nortriterpenoids, named schilancidilactones V-W (1-2). Their structures were elucidated by spectroscopic evidence. Compounds 1-2 feature a double bond between C-7 and C-8 compared with related known nortriterpenoids isolated from the genus Schisandra. Compounds 1 and 2 were tested for their anti-HIV-1 activities and cytotoxicity. The results revealed that compounds 1 and 2 showed moderate anti-HIV-1 activities with $EC_{50}$ 3.05 and 2.87 ${\mu}g/mL$, respectively, and compound 1 showed high cytotoxicity against KB and MDA-MB-231 cell with $IC_{50}$ values of 3.18 and 5.22 ${\mu}M$, respectively.

Astragali Radix: comprehensive review of its botany, phytochemistry, pharmacology and clinical application

Ya-Xiao Liu,Xiao-Mei Song,Lin-Wei Dan,Jia-Mei Tang,Yi Jiang,Chong Deng,Dong-Dong Zhang,Yu-Ze Li,Wei Wang 대한약학회 2024 Archives of Pharmacal Research Vol.47 No.3

Astragali Radix ( A. Radix ) is the dried root of Astragalus membranaceus var. mongholicus (Bge) Hsiao or Astragalusmembranaceus (Fisch.) Bge., belonging to the family Leguminosae, which is mainly distributed in China. A. Radix hasbeen consumed as a tonic in China for more than 2000 years because of its medicinal eff ects of invigorating the spleenand replenishing qi. Currently, more than 400 natural compounds have been isolated and identifi ed from A. Radix , mainlyincluding saponins, fl avonoids, phenylpropanoids, alkaloids, and others. Modern pharmacological studies have shown thatA. Radix has anti-tumor, anti-infl ammatory, immunomodulatory, anti-atherosclerotic, cardioprotective, anti-hypertensive,and anti-aging eff ects. It has been clinically used in the treatment of tumors, cardiovascular diseases, and cerebrovascularcomplications associated with diabetes with few side eff ects and high safety. This paper reviewed the progress of researchon its chemical constituents, pharmacological eff ects, clinical applications, developing applications, and toxicology, whichprovides a basis for the better development and utilization of A. Radix .

Model and Control of Novel Surface-Motor in Plane Motion

( Xue Peng Liu ),( Xue Song Mei ),( Xu Tang Wu ) 한국산업응용수학회(구 한국산업정보응용수학회) 2004 한국산업정보응용수학회 Vol.8 No.2

Efficacy and Safety of Neurokinin-1 Receptor Antagonists for Prevention of Chemotherapy-Induced Nausea and Vomiting: Systematic Review and Meta-analysis of Randomized Controlled Trials

Yuan, Dong-Mei,Li, Qian,Zhang, Qin,Xiao, Xin-Wu,Yao, Yan-Wen,Zhang, Yan,Lv, Yan-Ling,Liu, Hong-Bin,Lv, Tang-Feng,Song, Yong Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: Can addition of neurokinin-1 receptor antagonists (NK1-RAs) be considered as an ideal strategy for the prevention of chemotherapy-induced nausea and vomiting (CINV)? Researchers differ on this question. Materials and Methods: Electronic databases were searched for randomized control trials (RCTs) that evaluated the effectiveness and safety of NK1-RAs in preventing CINV. The primary end point was complete response (CR) in the acute, delayed, and overall phases after chemotherapy. Subgroup analyses evaluated the types of NK1-RAs, routines of administration, types of malignancies, regimens used in combination with NK1-RAs, and age of patients included in the studies. The incidences of different types of adverse events were also extracted to estimate the safety of NK1-RAs. Results: A total of 38 RCTs involving 13,923 patients were identified. The CR rate of patients receiving NK-RAs was significantly higher than patients in the control groups during overall phase (70.8% vs 56.0%, P<0.001), acute phase (85.1% vs 79.6%, P<0.001), and delayed phase (71.4% vs 58.2%, P<0.001). There were three studies including patients of children or adolescents, the CR rate was also significantly higher in the treatment group (overall phase: OR=2.807, P<0.001; acute phase: OR=2.863, P =0.012; delayed phase: OR=2.417, P<0.001). For all the other outcomes, patients in the NK1-RAs groups showed improvements compared to the control groups (incidence of nausea: 45.2% vs 45.9%, P<0.001; occurrence of vomiting: 22.6% vs 38.9%, P<0.001; usage of rescue drugs: 23.5% vs 34.1%, P<0.001). The pooled side effects from NK1-RAs did not significantly differ from previous reports and the toxicity rates in patients less than eighteen years old also did not diff between the two groups (P=0.497). However, we found that constipation and insomnia were more common in the patients of control groups, whereas diarrhea and hiccups were more frequently detected in patients receiving NK1-RAs. Conclusions: NK1-RAs improved the CR rate of CINV. They are effective for both adults and children. The use of NK1-RAs might be associated with the appearance of diarrhea and hiccups, while decreasing the possibility of constipation and insomnia.