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RISS 인기검색어
- 검색키워드
- 저자 : Taki Tiraihi (검색결과 2 건)
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Samane Adib,Taki Tiraihi,Merzieh Darvishi,Taher Taheri,Hadi Kazemi 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1
Human Bone marrow stromal cells (hBMSCs) can differentiate under appropriate experimental conditionsinto neuronal and glial-like cells. This study shows a protocol for producing human neural stem cells (hNSCs)from hBMSCs and the subsequent differentiation of hNSCs into cholinergic neurons (CNs), where sequential mediareplaced the culturing media. hBMSCs have been used in generating cell aggregates (CAs) using bFGF, EGF andB27. The hNSCs were isolated from CAs, and the CNs differentiated from the hNSCs using sequential media, wherebFGF, EGF and B27 were gradually replaced with NGF. The hNSC stemness was checked by RT-PCR of SOX2,Oct-4 and Nanog genes. Fibronectin, CD90, CD106, CD31, nestin, neurofilament 68 (NF-68), NF-200 and ChATimmunostaining evaluated the differentiation of the hBMSCs, the hNSCs and the CNs. FM1-43 was used in studyingthe function of the CNs. The hBMSCs were immunoreactive to fibronectin, CD90 and CD106; they werechecked for lipogenic and osteogenic differentiation. The cells of the CAs were immunoreactive to nestin. ThehNSCs were immunoreactive to nestin and NF-68, also, they expressed SOX2, Oct-4 and nanong. Nestin expressiondeclined sharply following NSC differentiation into CNs, while the expression of NF-200, synapsin I, synaptophysin,MAP-2 and ChAT increased. They were stained with FM1-43, where the synaptic vesicles were releasedfollowing stimulation. The present study demonstrates the conversion of hBMSCs into CASs under appropriate conditions. CAs generated hNSCs, which were induced in order to differentiate into CNs using sequential media, wherethe yield was 83%.
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Hamid Aboutaleb Kadkhodaeian,Taki Tiraihi,Hamid Ahmadieh,Hossein Ziaei,Narsis Daftarian,Taher Taheri 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.3
BACKGROUND: Retinal degeneration causes blindness, and cell replacement is a potential therapy. The purpose of this study is to formation of pigmented neurospheres in a simple medium, low-cost, high-performance manner over a short period of time while expressing markers of RPE cells and the activation of specific genes of the pigment cells. Also, these neurospheres have the ability to produce a monolayer of retinal pigment epithelium-like cells (RPELC) with the ability of photoreceptor outer segment phagocytosis. METHODS: BMSC were isolated from pigmented hooded male rats and were immunoreactive to BMSC markers, then converted into neurospheres, differentiated into pigmented spheres (PS), and characterized using Retinal pigment epithelium-specific 65 kDa protein (RPE65), Retinaldehyde-binding protein 1 (CRALBP) and orthodenticle homeobox 2 (OTX2) markers by immunocytochemistry, RT-PCR and RT-qPCR. The PS were harvested into RPELC. The functionality of RPELC was evaluated by phagocytosis of fluorescein-labeled photoreceptor outer segment. RESULTS: The BMSC immunophenotype was confirmed by immunostained for fibronectin, CD90, CD166 and CD44. These cells differentiated into osteogenic and lipogenic cells. The generated neurospheres were immunoreactive to nestin and stemness genes. The PS after 7–14 days were positive for RPE65 (92.76–100%), CRALBP (95.21–100%) and OTX2 (94.88–100%), and after 30 days RT-PCR, qPCR revealed increasing in gene expression. The PS formed a single layer of RPELC after cultivation and phagocyte photoreceptor outer segments. CONCLUSION: Bone marrow stromal stem cells can differentiate into functional retinal pigmented epithelium cells in a simple, low-cost, high-performancemanner over a short period of time. These cells due to expressing theRPELCgenes andmarkers can be used in cell replacement therapy for degenerative diseases including age-relatedmacular degeneration as well as retinitis pigmentosa.
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