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Kim, Taeyong,Kim, Jin Ock,Oh, Jae Gyun,Hong, Seong-Eui,Kim, Do Han Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1
Chronic pressure-overload cardiac hypertrophy is associated with an increased risk of morbidity/mortality, largely due to maladaptive remodeling and dilatation that progresses to dilated cardiomyopathy. Alternative splicing is an important biological mechanism that generates proteomic complexity and diversity. The recent development of next-generation RNA sequencing has improved our understanding of the qualitative signatures associated with alternative splicing in various biological conditions. However, the role of alternative splicing in cardiac hypertrophy is yet unknown. The present study employed RNA-Seq and a bioinformatic approach to detect the RNA splicing regulatory elements involved in alternative splicing during pressure-overload cardiac hypertrophy. We found GC-rich exonic motifs that regulate intron retention in 5' UTRs and AT-rich exonic motifs that are involved in exclusion of the AT-rich elements that cause mRNA instability in 3' UTRs. We also identified motifs in the intronic regions involved in exon exclusion and inclusion, which predicted splicing factors that bind to these motifs. We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy. Collectively, the present results suggest that chronic pressure-overload hypertrophy is closely associated with distinct alternative splicing due to altered expression of splicing factors.
납-비스무스 공융물 환경에서의 적층제조된 재료의 부식 거동 연구
김태용(Taeyong Kim),김병주(Byeongju Kim),김기동(Gidong Kim),송상우(Sangwoo Song),김지현(Ji Hyun Kim) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11
This paper study on LBE corrosion behavior of clad material manufactured by direct energy deposition . Fe-12Cr-2Si alloys, which is corrosion resistance material with clad, are immersed in 550 ℃, 1e-7 wt.% dissolved oxygen concentration static LBE. At the same time, SS316L clad are also exposed to same condition. Through a scanning electron microscope, corroded cross-section of clad Fe-12Cr-2Si, clad SS316L and as-received SS316L are compared. The clad Fe-12Cr-2Si shows more enhanced corrosion resistance than as-received Fe-12Cr-2Si because grain size of the clad is bigger so that diffusion paths like grain boundaries decrease. On the other hand, corrosion resistance of the clad SS316L is inferior to as-received SS316L. When passive layer is not sufficient, solub le Ni in LBE is easy to diffuse not only grain boundary but also grain. Larger grain which has high diffusivity along crystal plane locally, deeper the dissolution and LBE penetration depth.
납-비스무스 공융물 환경에서의 적층제조된 재료의 부식 거동 연구
김태용(Taeyong Kim),김병주(Byeongju Kim),김기동(Gidong Kim),송상우(Sangwoo Song),김지현(Ji Hyun Kim) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11
This paper study on LBE corrosion behavior of clad material manufactured by direct energy deposition . Fe-12Cr-2Si alloys, which is corrosion resistance material with clad, are immersed in 550 ℃, 1e-7 wt.% dissolved oxygen concentration static LBE. At the same time, SS316L clad are also exposed to same condition. Through a scanning electron microscope, corroded cross-section of clad Fe-12Cr-2Si, clad SS316L and as-received SS316L are compared. The clad Fe-12Cr-2Si shows more enhanced corrosion resistance than as-received Fe-12Cr-2Si because grain size of the clad is bigger so that diffusion paths like grain boundaries decrease. On the other hand, corrosion resistance of the clad SS316L is inferior to as-received SS316L. When passive layer is not sufficient, solub le Ni in LBE is easy to diffuse not only grain boundary but also grain. Larger grain which has high diffusivity along crystal plane locally, deeper the dissolution and LBE penetration depth.
넓은 온도 범위에서 고정 구동전류로 동작하는 1.25 Gbps 850 ㎚ 산화형 VCSEL 송신기의 특성
김태기(Taeki Kim),김태용(Taeyong Kim),김상배(Sang-Bae Kim),김성한(Sunghan Kim) 대한전자공학회 2007 電子工學會論文誌-SD (Semiconductor and devices) Vol.44 No.12
넓은 온도 범위에서 고정된 구동 전류로 동작하는 VCSEL(Vertical-Cavity Surface-Emitting Laser) 송신기의 저전류 동작 특성을 살펴보았다. 사용된 VCSEL은 산화형 850 ㎚ 레이저로, d²Ith/dT²값이 약 1.346×10?⁴ mA/℃² 으로 문턱전류의 온도의 존성이 비교적 낮은 것이었으며, 데이터 속도는 1.25 Gbps였다. 칩 상태에서의 광출력-전류-전압을 측정하여 20℃에서의 광출력이 1 mW가 되는 전류를 on 전류로 설정한 후 -20℃에서 80℃의 온도 범위에서 20℃ 간격으로 온도를 바꾸어가며 off 전류의 변화에 따른 turn-on 특성, turn-off 특성 그리고 eye-diagram을 관찰하였고 온도와 off 전류에 따라 eye-diagram에서 rise time, fall time, extinction ratio, timing jitter가 어떻게 변화하는지를 살펴보았다. 그 결과 최소 문턱전류보다 약 0.1~0.2mA 정도 낮게 off 전류를 설정하면 20℃에서 80℃의 온도 범위에서 off 전류와 on 전류를 변화시키지 않고 1.25 Gbps에서 송신기로 동작 시킬 수 있음을 확인하였다. We have analyzed low current operation characteristics of a VCSEL transmitter operating at fixed current over wide temperature range. Used 850 nm oxide VCSEL has low temperature dependence of the threshold current and d²Ith/dT² is approximately 1.346×10?⁴ mA/℃². We fixed on-current so that output power from the chip is 1 mW at 20℃ and investigated the turn-on, turn-off characteristics and eye-diagram of the 850 nm oxide VCSEL transmitter with varying ambient temperature and off-current. We measured rise time, fall time, extinction ratio and timing jitter by changing the ambient temperature and off-current. With the fixed off-current of around 0.1~0.2 mA lower than the lowest threshold current the transmitter successfully operated at 1.25 Gbps over a wide temperature range from 20℃ to 80℃.
Coxsackie and Adenovirus Receptor Binding Ablation Reduces Adenovirus Liver Tropism and Toxicity
Yun, Chae-Ok,Yoon, A-Rum,Yoo, Ji Young,Kim, Hoguen,Kim, Minjung,Ha, Taeyong,Kim, Gwi Eon,Kim, Hyunhee,Kim, Joo-Hang Mary Ann Liebert 2005 Human gene therapy Vol.16 No.2
<P>Human adenovirus-based vectors have emerged as a new promising vehicle for in vivo gene transfer-mediated therapy. However, the full potential of this methodology has not been fully realized because of the nonspecific tissue distribution of adenoviral vectors. Adenovirus infection is initiated by forming a complex between the fiber protein and a ubiquitously expressed host cell membrane protein, coxsackie B virus and adenovirus receptor (CAR). Therefore, ablating the adenovirus vector's ability to bind to the CAR is the first step in redirecting adenoviral tropism. To ablate CAR binding, we mutated the Bbeta sheet of the fiber knob, generating CAR-binding ablated replication-incompetent (dl-K420A-Z) and replication-competent (YKLK420A) adenoviral vectors. The in vitro transduction efficiency of dl-K420A-Z was significantly reduced in comparison to dl-LacZ carrying the wild-type fiber in CAR-positive cells but not in CAR-negative cells, suggesting that the mutation introduced in the Bbeta sheet of the fiber knob could disable the CAR-dependent transduction pathway. The in vivo transduction was also dramatically reduced in the liver and other organs for mice treated with dl-K420A-Z, compared with a cognate control vector, dl-LacZ. Concomitant with this attenuated gene transfer efficiency in vivo was a substantial reduction in the amount of general toxicity observed in the YKL-K420A-treated mice. Diminished toxicity was surmised from quantitative measurement of serum level of enzymes for liver and kidney function, hematologic chemistries, histopathology, and differences in lethality. Significant decrease in serum transaminases (alanine transferase [ALT] and aspartate transferase [AST]) was observed in mice treated with YKL-K420A. In addition, the lethality was lower in the YKLK420A- treated groups compared to the YKL-1-treated groups at all doses examined. Furthermore, the hepatopathologic analysis revealed that YKL-1 induced focal zonal necrosis and hepatocyte degeneration, while YKL-K420A induced mild spotty necrosis. In summary, this decreased vector tropism of CAR-binding ablated adenoviruses in normal tissues may increase the amount of virus available for infecting tumor cells and thus increase the antitumor efficacy with fewer unwanted side effects.</P>
Kim Dong Wook,Ahn Hyemin,Kim Kyung Won,Lee Seung Soo,Kim Hwa Jung,Ko Yousun,Park Taeyong,Lee Jeongjin 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.11
Objective: The clinical relevance of myosteatosis has not been well evaluated in patients with pancreatic ductal adenocarcinoma (PDAC), although sarcopenia has been extensively researched. Therefore, we evaluated the prognostic value of muscle quality, including myosteatosis, in patients with resectable PDAC treated surgically. Materials and Methods: We retrospectively evaluated 347 patients with resectable PDAC who underwent curative surgery (mean age ± standard deviation, 63.6 ± 9.6 years; 202 male). Automatic muscle segmentation was performed on preoperative computed tomography (CT) images using an artificial intelligence program. A single axial image of the portal phase at the inferior endplate level of the L3 vertebra was used for analysis in each patient. Sarcopenia was evaluated using the skeletal muscle index, calculated as the skeletal muscle area (SMA) divided by the height squared. The mean SMA attenuation was used to evaluate myosteatosis. Diagnostic cutoff values for sarcopenia and myosteatosis were devised using the Contal and O’Quigley methods, and patients were classified according to normal (nMT), sarcopenic (sMT), myosteatotic (mMT), or combined (cMT) muscle quality types. Multivariable Cox regression analyses were conducted to assess the effects of muscle type on the overall survival (OS) and recurrence-free survival (RFS) after surgery. Results: Eighty-four (24.2%), 73 (21.0%), 75 (21.6%), and 115 (33.1%) patients were classified as having nMT, sMT, mMT, and cMT, respectively. Compared to nMT, mMT and cMT were significantly associated with poorer OS, with hazard ratios (HRs) of 1.49 (95% confidence interval, 1.00–2.22) and 1.68 (1.16–2.43), respectively, while sMT was not (HR of 1.40 [0.94–2.10]). Only mMT was significantly associated with poorer RFS, with an HR of 1.59 (1.07–2.35), while sMT and cMT were not. Conclusion: Myosteatosis was associated with poor OS and RFS in patients with resectable PDAC who underwent curative surgery.