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Hypoallergenic and Physicochemical Properties of the A2 β-Casein Fraction of Goat Milk
Tae-Hwan Jung,Hyo-Jeong Hwang,Sung-Seob Yun,Won-Jae Lee,Jin-Wook Kim,Ji-Yun Ahn,Woo-Min Jeon,Kyoung-Sik Han2.6* 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.6
Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 β-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 β-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at 25°C). Selective reduction of β- lactoglobulin and αs-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 β-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 β-casein fraction. There was no significant difference in levels of both indicators between A2 β-casein treatment and the control (no protein treatment). The A2 β-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 β-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 β-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.
Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
Chaetoglobosin A, an Inhibitor of Bleb Formation on K562 Cells Induced by Phorbol 12,13-Dibutyrate
KO, HACK-RYONG,KIM, BO YEON,AHN, SOON-CHEOL,OH, WON KEUN,KIM, JIN-HEE,LEE, HYUN SUN,KIM, HWAN-MOOK,HAN, SANG-BAE,MHEEN, TAE0ICK,AHN, JONG-SEOG 한국미생물 · 생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.6
( Sang Yeob Lee ),( Yong Jun Kim ),( Sang Hyeon Kang ),( Dong Yeol Lee ),( Duk Song Cho ),( Kyong Han Kim ),( Won Tae Chung ),( Sung Won Lee ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1
The NSAIDs and celecoxib in the treatment of rheumatoid arthritis and osteoarthritis had been used for a long time.However, in vitro animal model, these drugs had been reported that inhibit PGE2 and suppressed the PGE2 production by osteoblasts is involved in the mechanism of bone resorption recently. so the influence of NSAIDs and celecoxib on the patient`s actual bone density was studied. We enrolled 445 patients (Rheumatoid arthritis 256, Osteoarthritis 189), and investigated an use of NSAIDs effects on bone mineral density. All patients included in the study of the patients receiving bisphosphonate. To evaluation of bone mineral density, the Hologic QDR dual energy x ray absorptiometry was regularly performed at intervals of one year, but one-year intervals to observe changes in bone mineral density thought to be too short observation period, two-year trend of change in bone mineral density measured. The NSAIDs administered in each of the patients and the NSAIDs have adverse effects on patients were treated on behalf of celecoxib. Both patients with rheumatoid arthritis, the average steroid prednisolone 10 mg / day was in use, the NSAIDs of the 140 people, the celecoxib was used the 116 patients. The patients with osteoarthritis did not use steroids, NSAIDs was used 106 patients, celecoxib was used 83 patients. In patients with rheumatoid arthritis, the average disease duration of 8 years, the average age is 70 years old and received treatment with NSAIDs or celecoxib period was an average of 4 years, in patients with degenerative arthritis, 7.8 years, 69 years old, was 3 years. During the observation period of the average change of the T-score showed increased in both groups. Patients with rheumatoid arthritis, lumbar portion of 0.11, hip portion 0.01, and patients with degenerative arthritis, lumbar portion 0.15, hip portion 0.06, were increased. Statistically in the groups with the administration of NSAIDs or celecoxib on bone mineral density did not affect. However, The use of bisposphonate was significant (p<0.05). In conclusion, the treatment of NSAIDs or celecoxib did not affect bone mineral density in rheumatoid arthritis patients or patients with degenerative arthritis.
Han, Ji-Wung,La, Tae-Min,Kim, Ji-Hoon,Choi, In-Soo,Song, Chang-Seon,Park, Seung-Yong,Lee, Joong-Bok,Lee, Sang-Won Elsevier 2018 INFECTION GENETICS AND EVOLUTION Vol.65 No.-
<P><B>Abstract</B></P> <P>We determined a complete genome sequence of the Korean field strain, KUMC-62, of human adenovirus type 3 (HAdV-3) and performed comparative genome analyses. Interestingly HAdV-3 has a distinct genomic sequence for the fiber CDS region on average 62.46% of nucleotide sequence identity to other types of HAdV-B1, while remaining genomic region of HAdV-3 is very similar (on average 95.71% of nucleotide sequence identity) to other types of HAdV-B1. The blast results showed that the fiber CDS region of HAdV-3 exhibited the highest nucleotide sequence identity with that of simian adenovirus type 32 (SAdV-32), except other strains of HAdV-3. In the Simplot analysis, a potential recombination event was detected between HAdV-7 and SAdV-32, which might have created HAdV-3 in the past. These findings suggest that HAdV-3 highly likely was created by a natural inter-species recombination event between human and non-human primate AdVs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> First complete genome sequencing of the Korean strain of human adenovirus type 3 using high-throughput sequencing technology. </LI> <LI> Shows distinct nucleotide sequences of the fiber CDS region of human adenovirus type 3 in comparative genome analysis. </LI> <LI> Evidence of multiple recombination events between adenoviruses from human and non-human primates. </LI> </UL> </P>
Transcriptome analysis of the endangered freshwater pearl bivalve Cristaria plicata
Tae Hun Wang,Bharat Bhusan Patnaik,Se Won Kang,Hee Ju Hwang,So Young Park,Eun Bi Park,Jong Min Chung,Dae Kwon Song,Changmu Kim,Soonok Kim,Jun Sang Lee,Yeon Soo Han,Hong Seog Park,Yong Seok Lee 한국수산해양기술학회(구 한국어업기술학회) 2015 한국수산해양기술학회 학술발표대회 Vol.2015 No.10
Improvement Effect of Soyeom Pharmacopuncture on Gout via NLRP3 Inflammasome Regulation
Sung Wook Kim,Jun Ho Lee,Hyeonjin Kim,Seong Hoon Lee,Dajeong Jeong,김혁순,이철중,Dae Yong Kim,Tae-han Yook,Gabsik Yang 대한약침학회 2022 Journal of pharmacopuncture Vol.25 No.4
Objectives: Gout is an inflammatory arthritis of the joints and soft tissues occurring due to deposition of monosodium urate (MSU) crystals, which are caused by persistent hyperuricemia. Soyeom pharmacopuncture is one treatment method that has been traditionally used for pain management in Oriental medicine. However, studies on its effect in reducing gout pain have been insufficient. Therefore, we selected Soyeom pharmacopuncture among natural products used in Korea as the new target of our study. Methods: The effects of Soyeom pharmacopuncture were examined in mouse models of acute gout induced by injection of MSU crystals into footpads. IL-1β, IL-6, and TNF-α production were examined by immunoblotting and enzyme-linked immunosorbent assay as hallmarks of NLRP3 inflammasome and cytokine activation. Results: Soyeom pharmacopuncture reduced foot edema in gout-induced mice, as well as IL-1β, nitrite, IL-6, and TNF-α production. Moreover, Soyeom pharmacopuncture also reduced MSU-induced gout inflammatory gene expressions, specifically those in the NF-kB pathway. Conclusion: Pharmacopuncture may serve as a new solution for other inflammatory diseases as well. Through active follow-up studies, we could thoroughly understand the clinical value of Soyeom pharmacopuncture.