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Lee Yong-Soo,Kim Tae-Young,Kim Yeji,Kim Seungil,Lee Su-Hyun,서상욱,Zhou Bo O.,Eunju O.,김광순,권미나 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Although functional interplay between intestinal microbiota and distant sites beyond the gut has been identified, the influence of microbiota-derived metabolites on hematopoietic stem cells (HSCs) remains unclear. This study investigated the role of microbiota-derived lactate in hematopoiesis using mice deficient in G-protein-coupled receptor (Gpr) 81 (Gpr81 − /− ), an established lactate receptor. We detected significant depletion of total HSCs in the bone marrow (BM) of Gpr81 −/− mice compared with heterogenic (Gpr81 +/− ) mice in a steady state. Notably, the expression levels of stem cell factor (SCF), which is required for the proliferation of HSCs, decreased significantly in leptin receptor-expressing (LepR + ) mesenchymal stromal cells (MSCs) around the sinusoidal vessels of the BM from Gpr81 −/− mice compared with Gpr81 +/− mice. Hematopoietic recovery and activation of BM niche cells after irradiation or busulfan treatment also required Gpr81 signals. Oral administration of lactic acid-producing bacteria (LAB) activated SCF secretion from LepR + BM MSCs and subsequently accelerated hematopoiesis and erythropoiesis. Most importantly, LAB feeding accelerated the self-renewal of HSCs in germ-free mice. These results suggest that microbiota-derived lactate stimulates SCF secretion by LepR + BM MSCs and subsequently activates hematopoiesis and erythropoiesis in a Gpr81-dependent manner.
Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development
Lee, Yong-Soo,Kim, Tae-Young,Kim, Yeji,Lee, Su-Hyun,Kim, Seungil,Kang, Sung Wan,Yang, Jin-Young,Baek, In-Jeoung,Sung, Young Hoon,Park, Yun-Yong,Hwang, Sung Wook,O, Eunju,Kim, Kwang Soon,Liu, Siqing,Ka Elsevier 2018 Cell host & microbe Vol.24 No.6
<P><B>Summary</B></P> <P>Symbionts play an indispensable role in gut homeostasis, but underlying mechanisms remain elusive. To clarify the role of lactic-acid-producing bacteria (LAB) on intestinal stem-cell (ISC)-mediated epithelial development, we fed mice with LAB-type symbionts such as <I>Bifidobacterium</I> and <I>Lactobacillus</I> spp. Here we show that administration of LAB-type symbionts significantly increased expansion of ISCs, Paneth cells, and goblet cells. Lactate stimulated ISC proliferation through Wnt/β-catenin signals of Paneth cells and intestinal stromal cells. Moreover, <I>Lactobacillus plantarum</I> strains lacking lactate dehydrogenase activity, which are deficient in lactate production, elicited less ISC proliferation. Pre-treatment with LAB-type symbionts or lactate protected mice in response to gut injury provoked by combined treatments with radiation and a chemotherapy drug. Impaired ISC-mediated epithelial development was found in mice deficient of the lactate G-protein-coupled receptor, Gpr81. Our results demonstrate that LAB-type symbiont-derived lactate plays a pivotal role in promoting ISC-mediated epithelial development in a Gpr81-dependent manner.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Symbiont-generated lactate is critical for Lgr5<SUP>+</SUP> ISC-mediated epithelial development </LI> <LI> Lactate signals through the G-protein-coupled receptor Gpr81 to elicit ISC proliferation </LI> <LI> <I>Lactobacillus plantarum</I> lacking lactate dehydrogenase fails to induce ISC regeneration </LI> <LI> Pre-feeding of lactate protects mice from chemotherapy- and radiation-induced gut damage </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>
Jo, Tae Geun,Lee, Jae Jun,Nam, Eunju,Bok, Kwon Hee,Lim, Mi Hee,Kim, Cheal The Royal Society of Chemistry 2016 NEW JOURNAL OF CHEMISTRY Vol.40 No.10
<P>A new 'turn-on fluorescence type'' chemosensor 1 (N'1,N'2-bis((E)-2-hydroxybenzylidene)oxalohydrazide) with a simple structure was devised and synthesized. In aqueous solution, receptor 1 could efficiently detect both Al3+ and CN- at two different wavelengths. The limit of detection for Al3+ (2.0 mu M) is below the World Health Organization (WHO) guideline for drinking water (7.41 mu M). To utilize for practical and biological applications, the ability of 1 for monitoring Al3+ was tested in real water samples and living cells. In addition, 1 showed a highly selective fluorescence enhancement for CN- in the presence of other anions without any interference. The sensing mechanisms of 1 for Al3+ and CN- were supported by theoretical calculations.</P>
무인선 및 GIS를 활용한 하천 내 클로로필-a와 수심의 공간 분석 및 시각화
김은주 ( Eunju Kim ),황태문 ( Tae-mun Hwang ),구재욱 ( Jaewuk Koo ),이새로미 ( Saromi Lee ),남숙현 ( Sookhyun Nam ) 한국수처리학회(구 한국수처리기술연구회) 2020 한국수처리학회지 Vol.28 No.5
This study is to develop Unmanned surface vehicle (USV) and apply it to rivers to analyze and visualize the space of chlorophyll-a and water depth. The results of the study showed that the chlorophyll-a and the depth of the water in the 2.31×10-<sup>1</sup> ㎢ of the N stream were successful. Data obtained from USV is stored in vast amounts in real time and must be converted and extracted into data that can be utilized through preprocessing programs. In this study, a preprocessing program using the exploratory data analysis (EDA) was implemented and used for converting and extracting data. The chlorophyll-a distribution divided the range based on the domestic water quality environment. As a result, 2.6% of Type Ia (chlorophyll-a 5 mg/㎥ or less), 9.9% of Type Ib (chlorophyll-a 9 mg/㎥ or less), 19.1% of Type II (chlorophyll-a 14 mg/㎥) and 27.9% of Type III (chlorophyll-a 20 mg/㎥ or below) and 27.9% of Type IV (chlorophyll- 35 mg/㎥ or below). Analysis of the water depth distribution showed that 15.8% were below 1m, 14.5% below 2 m, 16.4 percent below 3 m, 34.1% below 4 m, and 19.2% below 5 m. The results of spatial visualization by USV operation were successful. It demonstrated the potential for commercialization of remote monitoring technology development using USV in the future.
Antioxidant and antigenotoxic effect of dairy products supplemented with red ginseng extract
Park, Hanyeong,Lee, Minhee,Kim, Kee-Tae,Park, Eunju,Paik, Hyun-Dong Elsevier 2018 Journal of dairy science Vol.101 No.10
<P>The purpose of this study was to evaluate the antioxidant and antigenotoxic effect of dairy products milk (M) and yogurt (Y) after the addition of 2% red ginseng extract to milk (RM) and to yogurt (RY). Total phenolic content, total flavonoid content, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, oxygen radical absorbance capacity, and total radical trapping antioxidant potential were determined in the samples. Furthermore, antigenotoxic effect of samples was measured, using cornet assay in human leukocytes. Total phenolic content and total flavonoid content of RM [38.3 +/- 0.8 mg of gallic acid equivalents (GAE)/100 g, 23.6 +/- 0.1 mg of quercetin equivalents (QE)/100 g] and RY (41.1 +/- 0.9 mg of CAE/100 g, 18.7 +/- 0.1 mg of QE/100 g), respectively, were higher than those of M (6.31 +/- 0.2 mg of GAE/100 g, 10.4 +/- 0.1 mg of QE/100 g) and Y (8.1 +/- 0.9 mg of GAE/100 g, 8.4 +/- 0.2 mg of QE/100 g), respectively. The 2,2-diphenyl-l-picrylhydrazyl radical scavenging activity and oxygen radical absorbance capacity values increased significantly after the addition of 2% red ginseng in both. Additionally, the total radical trapping antioxidant potential in RM (787.7 +/- 7.0 mu g/mL) was lower than in M (2074.0 +/- 28.4 mu g/mL). The H2O2-induced DNA damage in RY (0.1 +/- 0.0 mg/mL) was less than the damage in Y (0.4 +/- 0.0 mg/mL), but we found no significant difference between M and RM. This study indicates that supplementation with red ginseng can fortify the antioxidant and antigenotoxic effects of dairy products effectively.</P>
In Vivo AAV1 Transduction With hRheb(S16H) Protects Hippocampal Neurons by BDNF Production
Jeon, Min-Tae,Nam, Jin Han,Shin, Won-Ho,Leem, Eunju,Jeong, Kyoung Hoon,Jung, Un Ju,Bae, Young-Seuk,Jin, Young-Ho,Kholodilov, Nikolai,Burke, Robert E,Lee, Seok-Geun,Jin, Byung Kwan,Kim, Sang Ryong Elsevier Science B.V., Amsterdam 2015 MOLECULAR THERAPY Vol.23 No.3
Bioconversion of Inositol Isomers by Enzymes and Microbial Fermentation
Hyelyeon HWANG,Eunju KIM,Ho Jae LEE,Chang Hee JEONG,Tae Woon KIM,Sung Wook HONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Inositol is a vitamin-like substance and found in foods such as fruits, beans, grains, and nuts. Inositol can be found in many isomers. The isomer D-chiro-inositol is a postreceptor signaling molecule of insulin action. However, the studies have documented that inositol in the ice plant (Delosperma) consist mainly of myo-inositol form, which is very low in biological utilization efficiency. The objective of this study was to investigate the optimal processing conditions for conversion of inositol into D-chiro-inositol form in the ice plant and to improve the biological utilization by enzyme treatment and fermentation. To convert inositol into D-chiro-inositol form, 0.5% (v/v) enzyme complexes(viscozyme, and celluclast), which consists of glucanase, xylanase, cellulase and hemicellulase was treated at 50℃ for 4h. In addition, fermentation was performed using an inositol 2-dehydrogenase producing strain, which was identified as Bacillus subtilis, Lacticaseibacillus casei, and L. paracasei by 16S rDNA analysis. The contents of D-chiro-inositol was significantly increased by enzyme treatment and fermentation in the ice plant. The results suggest that these methods to convert inositol to D-chiro-inositol can be applied for the use of ice plant processing as functional food biomaterial.
SCV를 장착한 4-밸브 스파크 점화기관의 유동 및 난류연소 3차원 해석
김우태(Woo Tae Kim),이은주(Eunju Lee),허강열(Kang Yul Huh) 한국자동차공학회 1997 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1997 No.11_1
Three-dimensional analysis of the in-cylinder flow has been performed for a 4-valve spark ignition engine with a swirl control valve. The mean velocities are compared with experimental data for three different SCV configurations. The calculation results are in good agreement except for one case during the compression stroke. The imposed upstream boundary condition seems to be the cause of this disagreement. Combustion calculation is performed using the Coherent Flamelet Model(CFM) for the reference case without an SCV. The results of the CFM seem to be correct qualitatively.<br/>
A Pilot Study on Characteristics of Metabolomics and Lipidomics according to Sasang Constitution
Kim, Min Jung,Lee, Da-Hye,Ahn, Jiyun,Ha, Tae-Youl,Jang, Young Jin,Do, Eunju,Jung, Chang Hwa Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-
<P>Although classification of an individual's Sasang constitution is a key step in the prescription of traditional Korean medicine, the classifying process is complex and not objective. Identification of metabolic-based biomarkers could allow the development of a reliable and sensitive classification technique and even therapeutic management. Our pilot study investigated whether metabolites in plasma are characteristic of Sasang constitutions. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based metabolic analysis was conducted against 15 Soyangin (SY), 15 Taeeumin (TE), and 18 Soeumin (SE) individuals, as classified according to the Questionnaire for Sasang Constitution Classification II (QSCC II) and specialist diagnosis. Metabolomics data showed that the TE group was significantly separated from the SY and SE groups. Nine canonical pathways related to constitution; phenylalanine metabolism, aminoacyl-tRNA, tyrosine, and tryptophan biosynthesis were activated in the TE group as compared with the other groups. Similar to the results of the metabolomics analysis, the TE group was also significantly separated from the other two groups by lipidomic analysis. On the other hand, the intensity of lipid metabolites was higher in the SY group than in the other groups. Our findings suggest that the combined analysis of metabolomics and lipidomics can provide useful information for characteristics of Sasang constitutions.</P>