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- 저자 : T. K. Chaitanya (검색결과 2 건)
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Cutting tool performance analysis of P/M alloy steel under turning of copper material
T. Panneerselvam,T. K. Kandavel,T. K. Chaitanya,K. S. Tarun 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.9
Automation in machining processes requires high strength new cutting tool to co-up with high speed, feed and high-temperature working conditions. The present work is aimed to analyze the performance of newly fabricated powder metallurgy (P/M) alloy steel, Fe-0.5 %C-2 %Cu-2 %Mo-2 %Ti as a cutting tool under turning of copper material using a conventional lathe. Taguchi’s experimental design matrix is used to conduct the experiment with cutting speed, depth of cut and feed rate as working parameters. The tool performance is assessed by evaluating the surface quality of the work material. Signal-to-noise ratio employs to optimize the working parameters. The surface roughness of copper work material machined by the P/M tool is a minimum at 43.175 m/min, 0.1 mm and 0.43 mm/rev of cutting speed, depth of cut and feed rate, respectively. Finally, the experimental analysis reveals that the P/M cutting tool will exhibit a comparable performance against the conventional HSS tool.
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B.N. Chaitanya,R. Asokan,T. Sita,K.B. Rebijith,P. Ram Kumar,N.K. Krishna Kumar 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Plutella xylostella (L.), Diamondback moth (DBM), is a notorious pest disrupting cruciferous crops across the world. Overuse of chemically synthesized insecticides as well as application of bio-pesticide like Bacillus thuringiensis has gained progressive resistance against them, resulting in DBM management failures. To overcome this, a more potential, non-chemical and safe technology like Ribonucleic acid interference (RNAi) is to be implemented. RNAi, is a reverse genetic tool with high sequence specificity, elicited by double-stranded RNA (dsRNA) for silencing the target genes. The objective of this study was to silence two candidate genes fromDBMviz., Juvenile Hormone Epoxide hydrolase (PxJHEH) and Ecdysteroid receptor (PxEcR) involved in regulating metamorphosis and molting respectively. In this regard, we have custom designed the dsRNAs (500 bp) by cloning and sequencing the candidate genes and delivered it orally (non-invasive mode). Further, we have assessed the extent of down regulation of target genes with five concentrations of dsRNA (1, 2, 5, 10 and 20 μg/μl) at four time points (24, 48, 72 and 96 h). The extent of gene silencing and mortality recorded for both genes were proportional to the dsRNA concentration. Our study projects that PxJHEH and PxEcR genes would be good target for dsRNA mediated gene silencing in management of insect pests such as DBM in near future.
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