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- 저자 : K.B. Rebijith (검색결과 2 건)
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B.N. Chaitanya,R. Asokan,T. Sita,K.B. Rebijith,P. Ram Kumar,N.K. Krishna Kumar 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Plutella xylostella (L.), Diamondback moth (DBM), is a notorious pest disrupting cruciferous crops across the world. Overuse of chemically synthesized insecticides as well as application of bio-pesticide like Bacillus thuringiensis has gained progressive resistance against them, resulting in DBM management failures. To overcome this, a more potential, non-chemical and safe technology like Ribonucleic acid interference (RNAi) is to be implemented. RNAi, is a reverse genetic tool with high sequence specificity, elicited by double-stranded RNA (dsRNA) for silencing the target genes. The objective of this study was to silence two candidate genes fromDBMviz., Juvenile Hormone Epoxide hydrolase (PxJHEH) and Ecdysteroid receptor (PxEcR) involved in regulating metamorphosis and molting respectively. In this regard, we have custom designed the dsRNAs (500 bp) by cloning and sequencing the candidate genes and delivered it orally (non-invasive mode). Further, we have assessed the extent of down regulation of target genes with five concentrations of dsRNA (1, 2, 5, 10 and 20 μg/μl) at four time points (24, 48, 72 and 96 h). The extent of gene silencing and mortality recorded for both genes were proportional to the dsRNA concentration. Our study projects that PxJHEH and PxEcR genes would be good target for dsRNA mediated gene silencing in management of insect pests such as DBM in near future.
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R. Asokan,K. B. REBIJITH,H. H. RANJITHA,H. K. ROOPA,V.V. Ramamurthy 한국곤충학회 2013 Entomological Research Vol.43 No.4
MicroRNAs (miRNAs) are a family of small, endogenously initiated non‐coding RNAs that extensively regulate gene expression either by mRNA cleavage or by translational repression, thus playing important roles in the development and physiology of organisms. Experimental identification and characterization of spatially and temporally expressed miRNAs is a monumental task in insects such as the brown plant hopper, Nilaparvata lugens, a major pest of rice and also a vector of Tenuivirus. At this juncture bioinformatics approaches, which primarily depend on sequence homology and secondary structure verification, will be an invaluable tool. A total of nine novel miRNAs were identified in N. lugens using the computational approaches from 1 13 718 Expressed Sequence Tags. The phylogenetic analysis was carried out to compare their level of conservation with respect to other members of the animal kingdom. The functional annotation of these newly identified miRNAs revealed that the majority of them are involved in the transcription regulatory activity and signal transduction pathways. These regulatory impacts are promising towards insect pest management but need further experimental validation and functional analyses. Thus, the outcome of this study will help to carry out the gene knockdown and transgenesis studies for the functional analysis of the newly identified miRNA‐regulated phenotypes in N. lugens.
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