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Antibiotics-Associated Hemorrhagic Colitis Caused by Klebsiella oxytoca: Two Case Reports
Youn, Youngmin,Lee, Sang Won,Cho, Hyun-Hae,Park, Sanghui,Chung, Hae-Sun,Seo, Jeong Wan The Korean Society of Pediatric Gastroenterology 2018 Pediatric gastroenterology, hepatology & nutrition Vol.21 No.2
Nowadays, Klebsiella oxytoca is described as a causative organism for antibiotic-associated hemorrhagic colitis (AAHC). Here we report two cases of pediatric AAHC, from which K. oxytoca was cultured after starting amoxicillin-clavulanate or amoxicillin treatment. The patients developed severe abdominal pain and a large amount of bloody diarrhea. K. oxytoca was obtained in intestinal fluid culture of a boy through the colonoscopy. On the other hand, colonic tissue culture and intestinal fluid culture were negative of the other patient. K. oxytoca was detected in stool culture when he was admitted. These cases showed characteristic endoscopic findings of segmental hemorrhagic colitis, and both boys recovered spontaneously within 2-3 days after they stopped taking the antibiotics. Therefore, in children who develop relatively large amount of bloody diarrhea after antibiotic treatment, we should consider AAHC caused by K. oxytoca.
Notes : Culture Conditions Affecting the Optimal Mycelial Growth of Cystoderma ami anthinum
( Sung Mi Shim ),( Yun Hee Oh ),( Kyung Rim Lee ),( Seong Hwan Kim ),( Kyung Hoan Im ),( Jung Wan Kim ),( U Youn Lee ),( Jae Ouk Shim ),( Mi Ja Shim ),( Min Woong Lee ),( Hyeon Su Ro ),( Hyun Sook Lee 한국균학회 2005 Mycobiology Vol.33 No.1
( Sang Youn Hwang ),( Seon-mi Lee ),( Jung Woo Im ),( Ki Jeong Jeon ),( Cheol-won Choi ),( Kyung-su Kim ),( Wan Jeon ) 대한간암학회 2019 대한간암학회지 Vol.19 No.1
Sorafenib is a well-known approved systemic therapeutic agent used in patients with advanced hepatocellular carcinoma (HCC). Regorafenib and nivolumab are approved as second-line therapeutic drugs in patients showing disease progression after sorafenib therapy. However, there is no established third- or fourth-line therapy in patients with progression after regorafenib or nivolumab treatment. Recently, the combination of tyrosine kinase inhibitors (TKIs) and immune checkpoint inhibitors (ICPIs) has been attempted as a firstline treatment strategy in advanced HCC patients based on the hypothesis that combination therapy may overcome resistance in ICPI monotherapy. On the basis of this suggestion, we herein describe the case of an HCC patient demonstrating macrovascular invasion, whereby partial remission was achieved via the combination of sorafenib and nivolumab following disease progression after nivolumab therapy. Further studies on the combination of TKIs and ICPIs are necessary to determine ways to manage HCC patients showing disease progression after ICPI therapy. (J Liver Cancer 2019;19:74-78)
Vertical Alignment of Carbon Nanotubes Using the Magneto-Evaporation Method
Youn, Sang Cheon,Jung, Dae-Hwan,Ko, Young Koan,Jin, Yong Wan,Kim, Jong Min,Jung, Hee-Tae American Chemical Society 2009 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.131 No.2
<P>We developed a novel method of vertical alignment of SWNTs using a single-step process for the simultaneous vertical alignment of the SWNTs by a magnetic field and the fixation of their alignment by means of the direct evaporation of the films. We fabricated Fe-oxide/SWNT samples that are reacted by iron-oleate complex, oleic acid and cut SWNTs in 1-octadecene. The Fe-oxide/SWNT samples are dispersed in N,N-dimethylformamide and the resulting solution was deposited on an ITO glass substrate using the spraying method with magnetic field. After evaporation of the SWNT solution in the presence of a magnetic field, we transferred the nanotubes to the vacuum-evaporator chamber, and titanium is evaporated by e-beam evaporation to hold the vertical alignment of the SWNTs. The resulting SWNTs exhibit the formation of a high degree of vertically aligned SNWTs over a large area. We showed that the degree of orientation of the SWNTs is strongly influenced by the field strength, film thickness of the evaporating molecules and evaporating rates. This technique takes significant advantages of the alignment of SWNTs with high aspect ratio at room temperature, without any organic binders and without the need for further alignment procedures. Moreover, this method might be applicable to other anisotropic materials with high aspect ratio.</P>
Youn-Kyung Ham,Dong-Heon Song,Sin-Woo Noh,Tae-Wan Gu,Jae-Hyeok Lee,Tae-Kyung Kim,Yun-Sang Choi,Hyun-Wook Kim 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.6
This study investigated the impacts of gelatin hydrolysate addition on the technological properties and lipid oxidation stability of cooked sausage. Gelatin hydrolysate was prepared from pork and duck skin gelatin, through stepwise hydrolysis using collagenase and pepsin. The cooked sausages were formulated without gelatin (control) or with 1% pork skin gelatin, 1% duck skin gelatin, 1% pork skin gelatin hydrolysate, and 1% duck skin gelatin hydrolysate. The pH, color characteristics, protein solubility, cooking loss, and textural properties of cooked sausages were evaluated, and the 2-thiobarbituric acid reactive substances (TBARS) value was measured weekly to determine lipid oxidation stability during 4 wk of refrigerated storage. Enzymatic hydrolysis of gelatin decreased protein content and CIE L* but increased redness and yellowness (p<0.05). When 1% gelatin or gelatin hydrolysate was incorporated in cooked sausage, however, little to no impacts on pH value, moisture content, protein content, color characteristics, protein solubility, and cooking loss were found (p>0.05). The addition of 1% duck skin gelatin hydrolysate increased the cohesiveness and chewiness of cooked sausages. The inclusion of 1% duck skin gelatin accelerated lipid oxidation of cooked sausages during refrigerated storage (p<0.05), whereas duck skin gelatin hydrolysate caused a lower TBARS value in cooked sausage compared to duck skin gelatin. The results show comparable effects of gelatin and gelatin hydrolysate addition on the technological properties of cooked sausages; however, the oxidative stability of raw materials for gelatin extraction should be evaluated clearly in further studies.
Sung, Hyeran,Chul Han, Kyung,Chul Kim, Jun,Wan Oh, Ki,Su Yoo, Hwan,Tae Hong, Jin,Bok Chung, Youn,Lee, Chong-kil,Lee, Kyung S.,Song, Sukgil ELSEVIER 2005 FEMS YEAST RESEARCH Vol.5 No.10
<P><B>Abstract</B></P><P>Functional analysis of genes from <I>Saccharomyces cerevisiae</I> has been the major goal after determination of genome sequences. Even though several tools for molecular-genetic analyses have been developed, only a limited number of reliable genetic tools are available to support functional assay at protein level. Epitope tagging is a powerful tool for detecting, purifying, and functional studying of proteins. But systematic tagging systems developed with integration vectors are not available. Here, we have constructed a set of integration vectors allowing a translational fusion of interested proteins to the four different epitope tags (HA, Myc, Flag, and GFP). To confirm function and expression of C-terminal-tagged proteins, we used Cdc11, a component of the septin filament that encircles the mother bud neck and consists of five major proteins: Cdc3, Cdc10, Cdc11, Cdc12, and Sep7. The tagged version of Cdc11 expressed under its endogenous promoter was found to be physiologically functional, as evidenced by localization at the neck and suppression of the growth defect associated with the temperature-sensitive mutation of <I>cdc11-6</I>. The expressed proteins were efficiently detected with antibodies against Cdc11 or the epitopes. When immunoprecipitated with anti-Myc antibody, each septin protein tagged with Myc was effectively copurified with other septin components, indicating formation of a stable septin complex. Because the modules of the tags were located under the same array of eighteen restriction sites on integration vectors containing four different markers (<I>HIS3</I>, <I>TRP1</I>, <I>LEU2</I>, or <I>URA3</I>), this tagging system provides efficient multiple tagging and stable expression of a gene of interest.</P>
Overexpression of PRAT1 protein is closely related to triple-negative breast cancer
Sang Eun Nam,Young-Sin Ko,Kyoung Sik Park,TongYi Jin,Young-Bum Yoo,Jung-Hyun Yang,Wook-Youn Kim,Hye-Seung Han,So-Dug Lim,Seung Eun Lee,Wan-Seop Kim 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.2
Purpose: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis and a lack of targeted therapy. Overexpression of PRAT1 is thought to be associated with this aggressive subtype of cancer. Here, we performed a comprehensive analysis and assessed the association between overexpression of PRAT1 and TNBC. Methods: First, using different web-based bioinformatics platforms (TIMER 2.0, UALCAN, and GEPIA 2), the expression of was assessed. Then, the expression of the PRAT1 protein and hormone receptors and HER2 status were assessed by immunohistochemical analysis. For samples of tumors with equivocal immunoreactivity, we performed silver in situ hybridization of the HER2 gene to determine an accurate HER2 status. Next, we used the R package and bc-GenExMiner 4.8 to analyze the relationship between PRAT1 expression and clinicopathological parameters in breast cancer patients. Finally, we determined the relationship between PRAT1 overexpression and prognosis in patients. Results: The expression of PRAT1 in breast cancer tissues is significantly higher than in normal tissue. PRAT1 expression was significantly related to worse overall survival (P < 0.05) and was correlated with these clinicopathological features: T stage, N stage, age, high histologic grade, estrogen receptor status, progesterone receptor status, Her-2 status, TNBC status, basal-like status, CK5/6 status, and Ki67 status. Conclusion: PRAT1 was overexpressed in breast cancer compared to normal tissue, and it may be involved in the progression of breast cancer malignancy. This study provides suggestive evidence of the prognostic role of PRAT1 in breast cancer and the therapeutic target for TNBC.
Index Structure and Algorithm for Path-based RDF Query
Sung Wan Kim,Youn Hee Kim,Hae Chull Lim 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7
Efficient processing of the queries on RDF data is one of the most important topics in RDF data management. To handle path-based query, Matono et al. proposed an index structure using suffix array. However they considered only the basic path expression in which it has the fixed length and there is no omitted component. In addition, they maintain the whole suffix labels in the single suffix array. If the size of RDF graph is increased the search scape to the suffix array is also increased. In this paper, we first modify Matono’s scheme to reduce the search space over index structure. Next we propose an query processing algorithm to handle some types of RDF queries not covered previously.