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- 저자 : Sung-Keun Cho (검색결과 2,074 건)
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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
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Cho, Jin Hyung,Kim, Sun Jai,Shim, June Sung,Lee, Keun-Woo The Korean Academy of Prosthodonitics 2017 The Journal of Advanced Prosthodontics Vol.9 No.2
PURPOSE. The aim of this study was to compare the surface roughness of zirconia when using Zircos E etching system (ZSAT), applying a nitric acid-hydrofluoric acid compound as a pretreatment agent, and also to compare the shear bonding strength according to different resin cements. MATERIALS AND METHODS. ZSAT, air abrasion, and tribochemical silicacoating were applied on prepared 120 zirconia specimens (10 mm in diameter, 7 mm in height) using CAD/CAM. Each 12 specimens with 4 different resin cements (Panavia F 2.0, Rely X Unicem, Superbond C&B, and Hot bond) were applied to test interfacial bond strength. The statistical analysis was performed using SAS 9.1 (SAS Institute Inc., Cary, NC, USA). The results are as follows: after application of the ZSAT on the zirconia specimens, surface roughness value after 2-hour etching was higher than those after 1- and 3-hour etching on SEM images. RESULTS. For Superbond C&B and Rely X Unicem, the specimens treated with ZSAT showed higher shear bond strength values than those treated with air abrasion and tribochemical silicacoating system. Regarding the failure mode of interface over cement and zirconia surface, Rely X Unicem and Hot bond showed cohesive failures and Panavia F 2.0 and Superbond C&B showed mixed failures. CONCLUSION. Zircos E etching system in zirconia restoration could increase its shear bond strength. However, its long term success rate and clinical application should be further evaluated.
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Cho Shinhaeng,Cho Ick Joon,Kim Yong Hyub,Jeong Jae-Uk,Yoon Mee Sun,Ahn Sung-Ja,Nam Taek-Keun,Song Ju-Young 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.5
The internal target volume (ITV)-based IMRT method can be used to reduce the effect of respiratory tumor motion. In this study, the effect of tumor motion on the dosimetric accuracy during ITV-based IMRT was investigated. ITV-based IMRT plans for a total of ten cases of liver cancer were prepared in TomoTherapy (Tomo) and volumetric modulated arc therapy (VMAT). Delivery quality assurance (DQA) plans were created using Delta4 phantom for the verification of dosimetric accuracy of the established Tomo and VMAT plans. DQA measurements were performed in both the static mode and the Hexa-Motion mode by using the Delta4 Hexa-Motion system in Tomo and VMAT. In the static mode, the average gamma passing rate (GPR) using a 3% dose difference/3 mm distance-to-agreement criteria was 99.61% in Tomo and 99.35% in VMAT, which confirmed sufficient dosimetric accuracy in both IMRT methods. For the Hexa-Motion measurement, the average GRP result of 90.93% in Tomo was higher than the result of 82.58% obtained in VMAT. In addition, the difference in the GPR results between the static and the Hexa-motion modes in Tomo was 8.68%, which is approximately 8% less than the 16.77% difference in VMAT. This study verifies that the dosimetric error of ITV-based IMRT due to the tumor motion was larger in VMAT than in Tomo. Also, Tomo can be considered to be more suitable than the conventional LINAC-based VMAT method in terms of the dosimetric accuracy of ITV-based IMRT for the treatment of a moving tumor.
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Time-Dependent Alginate/Polyvinyl Alcohol Hydrogels as Injectable Cell Carriers
Cho, Sang Ho,Lim, Sung Mook,Han, Dong Keun,Yuk, Soon Hong,Im, Gun Il,Lee, Jin Ho Informa UK (TaylorFrancis) 2009 Journal of Biomaterials Science. Polymer Edition Vol. No.
<P>Alginate hydrogels have been widely utilized as cell carriers due to their simplicity for fabricating cell-immobilized gel beads or 3-dimentional porous scaffolds, biocompatibility and non-toxicity to cells. Generally alginate hydrogels have been produced by contacting alginate solution with CaCl(2) as a cross-linking agent. However, the major disadvantages of this system are that the gelation rate is too fast and hard to control, and the prepared alginate gels cannot be injected. Injectable alginates have been prepared by using CaSO(4) or CaCO(3) as a cross-linking agent. However, the gelation rate of alginate with CaCO(3) is slow owing to the low solubility of CaCO(3) in water, while that with CaSO(4) is too fast to form uniform gels. In this study, we prepared injectable alginate/polyvinyl alcohol (PVA) blend hydrogels with controllable gelation rate by using CaSO(4) as a cross-linking agent and Na(2)HPO(4) as a cross-linking retardation agent. The gelation rate could be controlled by adjusting CaSO(4)/Na(2)HPO(4) ratio in the solution. The alginate and PVA showed good compatibility in aqueous solutions or gels. The gelation rate of alginate increased with increasing Na(2)HPO(4) and decreasing CaSO(4) concentrations, as expected. The PVA itself in the alginate/PVA blend did not affect the gelation rate. All alginate/PVA hydrogels demonstrated some extraction of PVA, but the extraction extent was not much even after 7 days immersion in water. The alginate/PVA hydrogels were examined for their in vitro cell compatibility by the culture of chondrocytes (human chondrocyte cell line) in the gels up to 28 days. The cells were grown almost linearly in the alginate/PVA hydrogels with higher PVA compositions showing better cell growth. The GAG contents from the cells in the hydrogels did not show dramatic changes with culture time, however, they also increased gradually in the alginate/PVA hydrogels with higher PVA composition. The PVA in the hydrogels seemed to play positive roles for the growth and activity of chondrocytes. The alginate/PVA hydrogels with controllable gelation rate are expected to be applicable as injectable cell carriers.</P>
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Cho, Sang-Hwan,Oh, Jeong Rok,Park, Hoo Keun,Kim, Hyoung Kun,Lee, Yong-Hee,Lee, Jae-Gab,Do, Young Rag The Optical Society 2010 Optics express Vol.18 No.2
<P>We demonstrate the combined effects of a microcavity structure and light-recycling filters (LRFs) on the forward electrical efficiency of phosphor-converted white organic light-emitting diodes (pc-WOLEDs). The introduction of a single pair of low- and high-index layers (SiO(2)/TiO(2)) improves the blue emission from blue OLED and the insertion of blue-passing and yellow-reflecting LRFs enhances the forward yellow emission from the YAG:Ce(3+) phosphors layers. The enhancement of the luminous efficacy of the forward white emission is 1.92 times that of a conventional pc-WOLED with color coordinates of (0.34, 0.34) and a correlated color temperature of about 4800 K.</P>
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( Keun Yong Kim ),( Myeong Hun Ko ),( Sung Jang Cho ),( Woo Jin Kim ),( Min Ho Son ),( In Chul Bang ) 한국수산과학회(구 한국수산학회) 2015 Fisheries and Aquatic Sciences Vol.18 No.1
A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5.22-bp differences (97.6.99.5% identities) between the hybrid and Pu. herzi, but as many as 158.168-bp differences (85.2.86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.
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Cloning of Two chitin Synthase Gene Fragments from Penicillium diversum
Cho, Seong-Pil,Lee, Sang-Keun,Lee, Dong-Hun,Bae, Kyung-Sook,Park, Hee-Moon,Maeng, Pil-Jae 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
The PCR fragments of two distinct chitin synthase genes, PdCHS1 and PdCHS2, were cloned from Penicillium diversum KCTC 6786. The nucleotide sequences of PdCHS1 and PdCHS2 contained uninterrupted open reading frames (ORFs) of 570 bp excluding the prime sequence. The similarity analysis of the deduced amino acid sequences using BLASTP indicated that the possible evolutionary relationship between P. diversum and ascomycetous fungi. Multialignment of the deduced amino acid sequences of PdCHSs using CLASTAL W revealed that the PdCHSs fell into two different classes: PdCHS1 into Class I and PdCHS2 into Class Ⅱ of chitin synthase defined by Bowen et al. (1992). By Southern blot analysis, it was shown that each of the two genes is present as a single copy in the genome of P. diversum KCTC 6786.
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Cho, Tae-Yeon,Lee, Won-Jae,Lee, Sang-Jin,Lee, Jae-Heung,Ryu, Juwhan,Cho, Seong-Keun,Choa, Sung-Hoon Elsevier 2018 THIN SOLID FILMS - Vol.660 No.-
<P><B>Abstract</B></P> <P>In this study, we demonstrated a single-layer silicon nitride (SiNx) film deposited on polyethyleneterephthalate substrate as a moisture permeation barrier film that reduced the water vapor transmission rate (WVTR). The SiNx film was fabricated by roll-to-roll, plasma-enhanced chemical vapor deposition (R2R-PECVD) in widths as large as 500 mm. The effects of the NH<SUB>3</SUB>/SiH<SUB>4</SUB> flow ratio on the SiNx properties were investigated according to their refractive index, optical characteristics, film density, WVTR, and chemical composition. The durability of the flexibility of the SiNx film was investigated via outer/inner bending test and cyclic bending fatigue test. The SiNx film showed excellent thickness uniformity and optical properties. At the NH<SUB>3</SUB>/SiH<SUB>4</SUB> flow ratio of 3, the SiNx film exhibited the highest film density and the best moisture barrier performances of 1.67 × 10<SUP>−3</SUP> g/m<SUP>2</SUP>·day. The results of the inner bending test showed that the flexibility of the SiNx film was excellent. The test results showed that the failure bending radius was 2 mm. The durable flexibility of the SiNx film was influenced by the internal residual stress and microstructure. After 10,000 cyclic inner-bending fatigue tests, no crack was observed, and the WVTR values were maintained below 2.0 × 10<SUP>−3</SUP> g/m<SUP>2</SUP>·day. These results indicate that the single-layer SiNx film fabricated with a simple R2R PECVD has a high potential as a moisture barrier film for future flexible electronic applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We demonstrate a single-layer silicon nitride (SiNx) film as permeation barrier film. </LI> <LI> SiNx film is coated by large roll-to-roll plasma enhanced chemical vapor deposition. </LI> <LI> Relationship between water permeation, flexibility and film density is first analyzed. </LI> <LI> It shows water permeation of 1.67 × 10<SUP>−3</SUP> g/m<SUP>2</SUP>·day and superior bendability. </LI> </UL> </P>
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