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Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site
Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6
The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.
실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교
황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2
Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.
싱글 튜브 시스템 BTA 工具를 使用한 SM55C 鋼의 深孔 加工 特性에 관한 硏究
全泰玉,全彦燦,沈成輔,張性圭 동아대학교 공과대학 부설 한국자원개발연구소 1994 硏究報告 Vol.18 No.2
The BTA deep hole machining has an increasing demand because of its wide applications and its good productivity. The main feature of the BTA drill is that the tool cutting edges are unsymmetrically located on the boring head. This provides a stabilizing cutting force resultant necessary for self guidance of the boring head. The BTA drills are capable of machining for having large length to diameter ratio in single pass. The study of the accuracy and surface finish of holes produced would reveal quite useful information regarding the process. This thesis deals with the experimental results obtained during single tube system BTA machining on SM55C steel for different machining conditions.
전성재,조연상,김동호,박흥식 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.1
It is known that fractal theory has recently been used as a useful in the characterization of surface texture and the understanding of tribological phenomena such as friction, wear and lubrication. The fractal-based method for describing the rubbed surface texture has roused great interest. In this paper, the fractal descriptors was applied to rubbed surface of hydraulic driving material with image processing system in order to describe morphology of the rubbed surface. The results showed that the fractal dimension can be determined by sum of intensity difference of surface pixel. The two step size to get fractal dimension is similar to surface roughness Ra. Fractal dimensions of the rubbed surfaces increase with an increase of applied load. Morphology of the rubbed surface driving in lubricant can be effectively obtained by fractal dimensions.
SGML을 이용한 제안서, 보고서 관리시스템 기술 개발
전병현,한치근,이상훈 경희대학교 산학협력기술연구원 1997 산학협력기술연구논문집 Vol.3 No.-
We designed an SGML-Based Proposal and Report Management System in this paper. In this system, SGML files are stored in a database and can be searched using keywords. Hence, the documents can be handled efficiently. The relational database structure is recommended for the system and also a hybrid approach is discussed for multimedia information in the text. Functions for each module of the system are defined and conceptual and practical design issues are provided.
社會福祉政策의 분석 : with respect to medical security in Korea 醫療保障制度를 中心으로
全相京 東亞大學校 大學院 1983 大學院論文集 Vol.7 No.-
Much emphasis had been laid upon social development since the fourth Five-Year Economic Plan. Along with this move to put emphasis upon social development, we are more concerned with social welfare than ever before. There are two views on social welfare: residual conception and institutional one. The residual conception of welfare lends weight to the conception that social welfare serves mainly such function wherein its activities are perceived as necessary only when those who are served by the "normal" institutional channels are unable to benefit, either through personel failings and exceptional needs or when those "normal" channels fail to perform appropriately. On the other hand, the institutional conception of welfare is as a distinct pattern of activities serving not a safety net to catch the pieces after all else has failed, but as an integral and "normal first line function of modern industrial society." Medical security is one of social welfare policies in functional views. And medical secutity consists of medical care and medical insurance. Medical care is a kind of public assistance which is conducted by the government revenue, while medical insurance is a sort of social insurance which aims the dispersion of social risk and income re-distribution. When we say about medical security from here on, we mean it both medical care and medical insurance. We analyze medical security using Gilbert and Specht's framework of social welfare policy. Their framework are as follows: the bases of eligibility of medical security benefits, the kind of medical security benefits, the mode of financing for medical security benefits and the delivery system of medical security benefits. To improve the relevance of this study, we should conduct field survey to distil useful information from the receipients of this policy, the authorities concerned with this policy and medical centers which deliver medical services to recipients. But due to many constraints, we depend upon only literary survey such as the acts concerned with medical security.
환경교육을 위한 웹 기반 프로젝트 학습 시스템의 설계 및 구현
전우천,정효숙,김동용,엄종진,정성모,최수경 서울교육대학교 2001 한국초등교육 Vol.12 No.2
The use of the Web (World Wide Web) has been changing roles of traditional education style. Especially, the Web can overcome time and space limitations in the traditional schools. Teachers and students are now using the Web to access vast amounts of information and resources on cyber space. Also, the learning via the Web enables both synchronous and asynchronous communication. Despite of many benefits of the Web, it may weaken students motivation due to lack of face-to-face communication. In this paper, we provide a learning model called Web Project Learning, which is based on the contructivism, to provide motivation and collaborative learning for students in the Web environment. The model is based on the Project-Based Learning model and is revised for use in the Web. The model can also encourage the participation of parents as well as students, and be applied to any subject. We implement our model and show that the model can be used for environmental education as an instance.
척추주위근을 침범한 신경통양 근위축증 (Neuralgic Amyotrophy) 2례
전포성,정상욱,김기찬,정호중 고신대학교 의학부 1998 高神大學校 醫學部 論文集 Vol.13 No.1-2
Neuralgic amyotrophy is a clinical syndrome of unknown etiology characterized by the acute onset of severe shoulder girdle pain followed by motor weakness or paralysis, and variable sensory loss. Up to 90 percent of patients eventually recover within the third years and the treatment is symptomatic. Neurologic findings and electrodiagnostic studies are only useful diagnostic methods of neuralgic amyotrophy and other laboratory testing are frequently normal or nonspecific. We report two cases of neuralgic amyotrophy with paraspinal muscles involvement with review of literature.
마이크로파 여기 플라즈마광 생성을 위한 도파관 시스템 설계에 관한 연구
전상재,전후동,송창현,하석영,이승혁,이태호,박의준 금오공과대학교 2005 論文集 Vol.26 No.-
In this paper, the plasma lighting system(PLS) excited by the commercial high power magnetron is developed. The design concepts are based on maximizing the huninous efficacy in conjunction with the miniaturization of waveguide system Furthermore the fine tuning is simplified by using only one stub, and the impedance matching is maximized by introducing the tapering technique. The experimental results show that the luminous efficacy can be dramatically improved by the proposed design method.