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Development of Plastid InDel Markers to Discriminate Lemons from Other Citrus Groups
Sang Suk Kim,Ho Bang Kim,Kyung Jin Park,Jae Wook Hyun,Cheol Woo Choi,Jae-Ho Joa,Seong Beom Jin,Eun-Sil Kim,Seung Gab Han 한국원예학회 2021 원예과학기술지 Vol.39 No.5
Lemon (Citrus limon), an interspecific hybrid between sour orange and citron, has been widely used as a rootstock along with trifoliate orange. Though lemons are superior to trifoliate orange in terms of their high seed germination rate throughout the year, one of the obstacles to using lemons as rootstocks is the lack of reliable, lemon-specific molecular markers to discriminate buds of the micro-grafted scion from those of the lemon rootstock. In order to obtain lemon-specific molecular markers, we compared the whole-plastid genomes available from four citrus species (lemon, pummelo, sweet orange, and mandarin) and developed seven plastid insertion/deletion (InDel) markers. The plastid InDel markers were applied to 46 citrus accessions that included lemons (17 accessions), grapefruit, mandarin, pummelo, sour orange, orange, papeda, tangor, and tangelo groups. The resulting dendrogram revealed that the citrus accessions used in this analysis could be distinctly classified into seven clusters. Lemons formed a separate cluster and had identical allele sizes for each InDel locus among all accessions investigated. This set of InDel markers could be a useful molecular tool for the rapid and clear discrimination of micro-grafted scions and lemon rootstocks during the production of virus-free citrus trees. The plastid InDel markers with maternal inheritance features can also be used to analyze the phylogenetic origin of various citrus cultivars including lemons.
Kim Chul,Sung Jidong,Han Jae-Young,Jee Sungju,Lee Jang Woo,Lee Jong Hwa,Kim Won-Seok,Bang Heui Je,Baek Sora,Joa Kyung Lim,Kim Ae Ryoung,Lee So Young,Kim Jihee,Kim Chung Reen,Kwon Oh Pum 대한의학회 2022 Journal of Korean medical science Vol.37 No.14
Background: In Korea, the actual distribution of cardiac rehabilitation (CR) to the clinical field is insufficient due to the many barriers for cardiovascular patients to participate in CR. Community-based CR is a useful alternative to overcome these obstacles. Through a nationwide survey, we investigated the possibility of regional medical and public health management institutes which can be in charge of community-based CR in Korea. Methods: The questionnaires on recognition of CR and current available resources in health-related institutions were developed with reference to the CR evaluation tools of York University and the International Council of Cardiovascular Prevention and Rehabilitation. The questionnaires were sent to regional public and private medical institutions and public health management institutions. Results: In total, 2,267 questionnaires were sent to 1,186 institutions. There were 241 and 242 responses from 173 and 179 regional private and public medical institutions, respectively. And a total of 244 responses were gathered from 180 public health management institutions. Although many institutions were equipped with the necessary facilities for exercise training, there were few patient-monitoring systems during exercise. Most institutions were aware of the need for CR, but were burdened with the cost of establishing personnel and facilities to operate CR. Conclusion: Most regional medical, and public health management institutions in Korea are unprepared for the implementation of community-based CR programs. To encourage the utilization of such, there should be efforts to establish a national consensus.
Kim, Hyo-Sang,Jung, Han Young,Kim, Myeong-Ok,Joa, Kyung-Lim,Kim, Yeo Ju,Kwon, Su-Yeon,Kim, Chang-Hwan Wolters Kluwer Health 2015 Medicine Vol.94 No.5
<P><B>Abstract</B></P><P>Bisphosphonates have been commonly used for the treatment of osteoporosis. However, there have been recent case reports of atypical fractures citing their long-term use, which inhibits the turnover of bone components.</P><P>A 64-year-old woman visited the outpatient clinic with pain in her right thigh and ambulation difficulty. We found fractures at both pedicles of L4 vertebra. subtrochanteric region of right femur, and left femoral shaft upon a radiologic examination. She had taken intravenous ibandronic sodium for osteoporosis over 3 years.</P><P>We changed the bishophonates to a parathyroid hormone because it was suspected that the multiple fractures were caused by the medication. Further, rehabilitation, including progressive weight bearing, was started. After 3 months of the conservative treatment, she was able to walk independently.</P><P>In conclusion, it is necessary to evaluate the possibility of atypical fractures in osteoporotic patients when they complain of lower extremity pain and to consider alternative treatments instead of bisphosphonates.</P>