Enhanced percutaneous delivery of recombinant human epidermal growth factor employing nano-liposome system

Jeon, Sang-Ok,Hwang, Hee-Jin,Oh, Dong-Ho,Seo, Jo-Eun,Chun, Kyeung-Hwa,Hong, Sun-Mi,Kim, Min-Ju,Kim, Won-Chul,Park, Min-Sun,Yoon, Chae-Ha,Min, Kyung-Hyun,Suh, Chang-Woo,Lee, Sangkil Informa UK, Ltd. 2012 Journal of microencapsulation Vol.29 No.3

<P>We encapsulated recombinant human epidermal growth factor (rhEGF) into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoViva™). Particle size, zeta (<I>ζ</I>) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, zeta potential and encapsulation efficiency of the NLs were 155.57 ± 2.59 nm, −57.92 ± 4.35 mV and 9.00 ± 0.39%%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin.</P>

Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts

Jeon, Young-Mi,Kook, Sung-Ho,Son, Young-Ok,Kim, Eun Mi,Park, Soon-Sun,Kim, Jong-Ghee,Lee, Jeong-Chae Springer-Verlag 2009 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.320 No.1

ISG15 modification of filamin B negatively regulates the type I interferon‐induced JNK signalling pathway

Jeon, Young Joo,Choi, Joon Seok,Lee, Jung Yun,Yu, Kyung Ryun,Kim, Sangman Michael,Ka, Seung Hyeun,Oh, Kyu Hee,Il Kim, Keun,Zhang, Dong‐,Er,Bang, Ok Sun,Ha Chung, Chin EMBO 2009 EMBO reports Vol.10 No.4

<P>Interferon (IFN)-induced signalling pathways have essential functions in innate immune responses. In response to type I IFNs, filamin B tethers RAC1 and a Jun N-terminal kinase (JNK)-specific mitogen-activated protein kinase (MAPK) module--MEKK1, MKK4 and JNK--and thereby promotes the activation of JNK and JNK-mediated apoptosis. Here, we show that type I IFNs induce the conjugation of filamin B by interferon-stimulated gene 15 (ISG15). ISGylation of filamin B led to the release of RAC1, MEKK1 and MKK4 from the scaffold protein and thus to the prevention of sequential activation of the JNK cascade. By contrast, blockade of filamin B ISGylation by substitution of Lys 2467 with arginine or by knockdown of ubiquitin-activating enzyme E1-like (UBEL1) prevented the release of the signalling molecules from filamin B, resulting in persistent promotion of JNK activation and JNK-mediated apoptosis. These results indicate that filamin B ISGylation acts as a negative feedback regulatory gate for the desensitization of type I IFN-induced JNK signalling.</P>

Muramyl dipeptide potentiates a <i>Bacillus anthracis</i> poly-γ-<small>D</small>-glutamic acid capsule surrogate that induces maturation and activation of mouse dendritic cells

Jeon, Jun Ho,Park, Deok-Bum,Woo, Sun-Je,Lee, Hae-Ri,Park, Ok-Kyu,Park, Jungchan,Rhie, Gi-eun Elsevier 2018 Cytokine Vol.110 No.-

<P><B>Abstract</B></P> <P>Poly-γ-<SMALL>D</SMALL>-glutamic acid (PGA) of anthrax is an important pathogenic factor due to its anti-phagocytic activity. Additionally, PGA has the ability to activate mouse macrophages for the secretion of cytokines through Toll-like receptor (TLR) 2. Peptidoglycan (PGN), a major bacterial cell-wall component, induces inflammatory responses in the host. We assessed whether PGA can induce maturation and cytokine expression in immature mouse dendritic cells (DCs) in the existence of muramyl dipeptide (MDP), the minimum motif of PGN with immunostimulatory activity. Stimulation of immature DCs with PGA or MDP alone augmented expression of costimulatory molecules and MHC class II proteins, which are all cell surface markers indicative of maturation. The observed effects were further enhanced by costimulation of PGA and MDP. PGA alone was sufficient to induce expression of TNF-α, IL-6, MCP-1, and MIP1-α, whereas MDP alone did not under the same conditions. Treatment with MDP enhanced PGA-induced expression of the tested inflammatory mediators; however, the synergistic effect found for PGA and MDP was not observed in TLR2- or nucleotide-binding oligomerization domain (NOD) 2–knockout DCs. Additionally, MDP augmented PGA-induced MAP kinases and NF-κB activation, which is crucial for expression of cytokines. Furthermore, MAP kinase and NF-κB inhibitors attenuated MDP enhancement of PGA-induced cytokine production. In addition, co-culture of splenocytes and PGA/MDP-matured DCs induced higher expression of IL-2 and IFN-γ compared to that of splenocytes and PGA-matured DCs. Collectively, our results suggest that PGA and MDP cooperatively induce inflammatory responses in mouse DCs through TLR2 and NOD2 via MAP kinase and NF-κB pathways, subsequently leading to lymphocyte activation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MDP enhances PGA-induced maturation of mouse DCs. </LI> <LI> MDP enhances production of inflammatory mediators by PGA. </LI> <LI> TLR2, NOD2, MAP kinase, and NF-κB signaling pathways are involved in PGA/MDP-induced cytokine production. </LI> <LI> MDP increases lymphocyte activation induced by PGA-matured DCs. </LI> </UL> </P>

전북지역 중년여성의 건강염려, 건강증진행동 및 삶의 질에 대한 연구

전선영(Sun Young Jeon),정성석(Sung Suk Chung),노정옥(Jeong Ok Rho) 한국영양학회 2020 Journal of Nutrition and Health Vol.53 No.6

본 연구는 전북지역 334명의 중년여성을 대상으로 건강염려, 건강증진행동 및 삶의 질의 수준을 조사하였으며, 그 결과를 요약하면 다음과 같다. 건강염려의 총점은 60점 중 37.64점으로 보통수준, 전체 평균값은 3.14점이었다. 하위항목 중 건강습관이 3.51점으로 가장 높았다. ‘흡연과 같은 해로운 습관을 피한다’가 4.04점으로 가장 높으며, ‘건강에 대하여 걱정한다’가2.30점으로 가장 낮았다. 건강증진의 총점은 115점 중 79.18점으로 보통수준, 전체 평균값은 3.44점이었다. 하위항목 중 생활습관이 4.09점으로 가장 높았다. 문항 중 ‘흡연을 하지 않는다’가 4.25점으로 가장 높으며, ‘미역, 김, 다시마, 멸치, 우유 등을 자주 섭취한다’가 2.48점으로 가장 낮았다. 삶의 질의 총점은 150점 중 101.18점으로 보통수준이며, 전체 평균값은 3.37점이었다. 하위항목 중 사회적 요인이 3.63점으로 가장 높았다, ‘자녀와의 관계만족’이 3.72점으로 가장 높았으며, ‘전반적인 건강상태 만족’이 2.95점으로 가장 낮았다. 조사대상자의 일반적인 특성에 따른 건강염려는 ‘BMI’, ‘가계월수입’, ‘직업’, ‘질병여부’, ‘체중만족’, ‘체중조절관심’에서 유의한 차이가 있었다. 건강증진행동은 ‘연령, ‘BMI’, ‘가계월수입’, ‘생리여부’, ‘건강보조식품섭취’, ‘체형인식’, ‘체형만족도’에서 유의한 차이가 있었다. 삶의 질은 ‘BMI’, ‘가계월수입’, ‘학력’, ‘직업’, ‘질병여부’, ‘체중만족’에서 유의한 차이가 있었다. 삶의 질에 미치는 관련변수들의 상대적 영향력을 알아본 결과, 삶의 질에 대한 전체 1단계에서 BMI ‘18.5 이상 23.0 미만’, 질병 ‘없음’, 가계월수입 ‘500만원 이상’의 순이며, 설명력은 17.4%이었다. 2단계에서 건강염려 요인 투입에 따른 총 설명력은 23.2%이며, ‘건강염려’의 영향력이 가장 크며, 그 다음은 질병 ‘없음’, 가계월수입 ‘500만원 이상’의 순이었다. 3단계 건강증진행동 요인 투입에 따른 총 설명력은 40.8%이었다. 삶의 질에 대한 예측변인의 영향력은 ‘건강증진행동’, 질병 ‘없음’, ‘건강염려’의 순이었다. 이상의 결과, 전북지역 중년여성의 건강염려 수준과 건강증진 행동은 보통수준이었다. 그러나 건강증진행동은 실제 삶의 질에 큰 영향력을 가지고 있었다. 특히, 질병 없는 건강상태는 건강증진행동을 실천하는데 중요한 동기부여가 될 수 있다. 따라서 건강염려 수준을 적절히 유지하며, 신체적 변화기에 있는 중년여성들의 건강관리 능력을 높이는 것이 삶의 질을 높이는 방안이라 판단된다. Purpose: The purpose of the study was to identify the health anxiety issues of middleaged women, their health-promoting behavior, and quality of life as well as to examine the relationship between these variables. Methods: The participants were 334 women in Jeonbuk area. Demographic characteristics, the status of health anxiety, health-promoting behavior, and life quality was assessed using a self-administered questionnaire. The data were analyzed using a t-test, analysis of variance, Duncan test, and hierarchical regression analysis with SPSS ver. 24.0. Results: The score for health anxiety was 37.64 points out of a possible score of 60, and the score for health-promoting behavior was 79.18 points out of a possible score of 115. The score for the quality of life was 101.18 points out of a possible score of 150. The health anxiety scores showed significant differences, varying as per body mass index (BMI) (p < 0.05), income (p < 0.05), occupation (p < 0.05), disease (p < 0.05), satisfaction with weight (p < 0.05), and interest in weight control (p < 0.05). The health-promoting behavior showed significant differences according to age (p < 0.01), BMI (p < 0.01), income (p < 0.05), menses (p < 0.05), intake of dietary supplements (p < 0.05), perception of body image (p < 0.05), and satisfaction with weight (p < 0.05). The quality of life showed significant differences according to BMI (p < 0.05), income (p < 0.01), education level (p < 0.05), occupation (p < 0.05), disease (p < 0.05), and satisfaction with weight (p < 0.05). Regression analysis showed that health-promoting behavior was the most influential variable on the quality of life, followed by disease and health anxiety. Conclusion: Based on these results, we conclude that it is necessary to consider educational programs on improving the quality of life of middle-aged women according to the health anxiety levels and health-promoting behavior.

프랜차이즈 미용실에서의 체험마케팅이 브랜드 태도와 브랜드 충성도에 미치는 영향

임순녀 ( Sun Nye Lim ),채희옥 ( Hee Ok Chae ),전현진 ( Hyun Jin Jeon ) 한국패션비즈니스학회 2014 패션 비즈니스 Vol.18 No.5

This study examined the relative importance level of experience marketing on brand attitude and brand loyalty in beauty salon franchise stores, and attempted to raise the competitiveness of beauty salon services by presenting this elements as experience marketing strategy directions for beauty salon franchise store management. A questionnaire was used for this study on 350 adults residing in Gwangju Metropolitan City and SPSS 20.0 statistics package was used for analysis. In summary, the results of this study are as follows. First, five factors that make up experience marketing in beauty salon franchise stores were found. They were sensory marketing, relative marketing, emotional marketing, cognitive marketing and behavior marketing. Brand loyalty was categorized into the two factors, attitude loyalty and behavioral loyalty. Also, we found that experience marketing had a statistically positive impact on brand loyalty. Secondly, experience marketing of beauty salon franchise stores was found to have a statistically significant positive effect on brand attitude. Thirdly, we found that the brand attitude of beauty salon franchise stores had a statistically significant positive effect on brand loyalty.

대학생의 부모자녀관계에 대한 인식이 주관적 안녕감과 심리적 안녕감에 미치는 영향

전선옥 ( Jeon Sun Ok ),김정원 ( Kim Jungwon ) 열린부모교육학회 2016 열린부모교육연구 Vol.8 No.4

본 연구에서는 대학생이 인식하고 있는 부모자녀관계가 이들의 주관적 안녕감과 심리적 안녕감에 미치는 영향을 알아보았다. 강원도에 위치한 H대학의 학부 5개 학과의 1∼3학년에 재학하고 있는 대학생 337명을 대상으로 한국적 부모자녀관계인 부자유친성정, 주관적 안녕감, 그리고 심리적 안녕감을 묻는 질문지를 사용하여 조사한 후 평균과 표준편차를 구하고, 상관관계분석과 회귀분석을 통해 밝혀낸 연구의 결과는 다음과 같다. 첫째, 부모자녀관계에 대한 대학생의 인식과 주관적 안녕감 간에는 긍정적인 상관관계가 있는 것으로 나타나 대학생들이 부모와의 일체감과 부모의 헌신성을 높은 수준으로 인식하고 있을수록 대학생들의 주관적 안녕감의 수준도 높아지는 것으로 나타났다. 단계적 중다회귀분석 결과 어머니와의 일체감과 헌신성에 대한 인식, 그리고 아버지와의 일체감과 헌신성에 대한 인식은 모두 대학생들의 주관적 안녕감에 많은 영향을 미치고 있었고 특히 어머니와의 일체감과 헌신성에 대한 인식이 더 많은 영향력을 미치는 것으로 밝혀졌다. 둘째, 부모자녀관계에 대한 대학생의 인식과 심리적 안녕감 간에는 긍정적인 상관관계가 있는 것으로 나타나 대학생들이 부모와의 일체감과 부모의 헌신성을 높은 수준으로 인식하고 있을수록 대학생들의 심리적 안녕감의 수준도 높아지는 것으로 나타났다. 단계적 중다회귀분석 결과 아버지와의 일체감과 헌신성, 그리고 어머니와의 일체감과 헌신성이 대학생들의 심리적 안녕감에 많은 영향을 미치고 있었고 특히 아버지와의 일체감과 헌신성에 대한 인식이 더 많은 영향력을 미치는 것으로 밝혀졌다. This study investigated the effects of college students` recognition with their parents` sense of unity and about their parents` devotion to them on their subjective happiness and psychological well-being. The participants in this study included 337 college students who have various majors in a college in Chunchun, Gangwon-Do, Korea. These college students participated in a survey on their perception of their recognition with their parents` sense of unity and about their parents` devotion to them, subjective happiness, and psychological well-being. The results of this study are as follows. First, the correlation analysis revealed that college students` recognition with their parents` sense of unity and about their parents` devotion to them and their subjective happiness were highly correlated. Stepwise regression analysis showed that college students` recognition with their mothers` sense of unity and about their mothers` devotion to them, and their fathers` sense of unity and about their fathers` devotion to them influenced their subjective happiness. Second, the correlation analysis revealed that college students` recognition with their parents` sense of unity and about their parents` devotion to them and their psychological well-being were highly correlated. Stepwise regression analysis showed that college students` recognition with their fathers` sense of unity and about their fathers` devotion to them, and their mothers` sense of unity and about their mothers` devotion to them influenced their psychological well-being.

객체지향 모형 간 일관성 검증을 지원하는 CASE 도구 설계 및 구현

이선미(Sun Mi Lee),전진옥(Jin Ok Jeon),류재철(Jae Chol Ryou) 한국정보처리학회 1999 정보처리학회논문지 Vol.6 No.11

A deductive database consists of facts being the extensional database and rules being the intensional database. Because of the difficulty of evaluating rules, many parallel evaluation algorithms for rules have beeen presented. But we hvae not gotten an acceptable result. This paper proposes a new methoddology to evaluate proof-theoretic meaning of the linear recursion rule which contains transitive dependency by using a shared-nothing parallel architecture. At first, we prove that there exists the equivalent expression for a linear recursion rule, design the algorithm for evaluating the linear recursion rule based on the equivalent expression, and finally analyse performance of the proposed algorithm.

사람면역결핍바이러스 감염에 동반된 미만성 침윤성 림프구 증가 증후군

권선옥 ( Sun Ok Kwon ),박원우 ( Won Wo Park ),이현경 ( Hyun Kyung Lee ),이성순 ( Sung Soon Lee ),강윤경 ( Youn Kyung Kang ),이영민 ( Young Min Lee ),이혁표 ( Hyuk Pyo Lee ),김주인 ( Joo In Kim ),최수전 ( Soo Jeon Choi ),염호기 ( H 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.3

Diffuse infiltrative lymphocytosis syndrome is an autoimmune syndrome that is characterized by the oligoclonal expansion of CD8+ T-lymphocytes in response to human immunodeficiency virus (HIV) antigens. The clinical manifestations include bilateral enlargement of the parotid glands, lymphocytic interstitial pneumonitis, lymphocytic hepatitis, neurological involvement and systemic lymphadenopathies. In addition to a positive HIV test, the diagnostic histopathological findings are CD8+ T-lymphocytic infiltrations in the lymphnodes, liver, lung, muscle and the salivary or lacrimal glands without granulomatous or neoplastic involvement. We report a case of pulmonary involvement of diffuse infiltrative lymphocytosis syndrome that was associated with a human immunodeficiency virus infection. (Tuberc Respir Dis 2006; 61: 289-293)

고추의 종류 및 부위에 따른 돌연변이유발 억제효과

최선미(Sun Mi Choi),전영수(Young Soo Jeon),박건영(Kun Young Park),정근옥(Keun Ok Jung) 부산대학교 김치연구소 2001 김치의 과학과 기술 Vol.7 No.-

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