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Suji Jeong,Borim An,Jung-Hyun Kim,Hyo-Won Han,Jung-Hyun Kim,Hye-Ryeon Heo,Kwon Soo Ha,한은택,Won Sun Park,홍석호 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
The efficient and reproducible derivation and maturation of multipotent hematopoietic progenitors from human pluripotent stem cells (hPSCs) requires the recapitulation of appropriate developmental stages and the microenvironment. Here, using serum-, xeno-, and feeder-free stepwise hematopoietic induction protocols, we showed that short-term and high-concentration treatment of hPSCs with bone morphogenetic protein 4 (BMP4) strongly promoted early mesoderm induction followed by increased hematopoietic commitment. This method reduced variations in hematopoietic differentiation among hPSC lines maintained under chemically defined Essential 8 medium compared to those maintained under less-defined mTeSR medium. We also found that perivascular niche cells (PVCs) significantly augmented the production of hematopoietic cells via paracrine signaling mechanisms only when they were present during the hematopoietic commitment phase. A protein array revealed 86 differentially expressed (>1.5-fold) secretion factors in PVC-conditioned medium compared with serum-free control medium, of which the transforming growth factor-β inducible gene H3 significantly increased the number of hematopoietic colony-forming colonies. Our data suggest that BMP4 and PVCs promote the hematopoietic differentiation of hPSCs in a differentiation stage-specific manner. This will increase our understanding of hematopoietic development and expedite the development of hPSC-derived blood products for therapeutic use.
Suji Shin,Sung-Eon Kim,Seong-Won An,Seong-Mok Jeong,Young-Sam Kwon The Korean Society of Veterinary Science 2024 大韓獸醫學會誌 Vol.64 No.1
This study was performed to assess the antiapoptotic effect of canine platelet-rich plasma (PRP) treated on the canine adipose-derived mesenchymal stem cells (cMSCs) under cold ischemic conditions. The effect of preventing apoptosis of cMSCs was evaluated in the apoptotic condition induced by cold ischemic injury in vitro. To determine the progression of apoptosis, the changes in cell nucleus were observed using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining. In addition, we examined the mitochondrial membrane potential (MMP) and caspase-3 activity. When the cold hypoxic injury was applied to cMSCs, the apoptotic change was observed by DAPI staining, mitochondrial staining for MMP, and caspase-3 assay. PRP significantly decreased the number of apoptotic cells. Nuclear shrinkage and fragmentation of apoptotic cells in control groups were observed by DAPI staining. The MMP was recovered by the treatment of PRP. In addition, when the luminescence intensity was measured for caspase-3 activity, the value was significantly higher in the PRP treated groups than the control groups. The results of this study showed that the PRP may have a beneficial effect on apoptosis induced by cold ischemic injury.
Metabolic Engineering for Improved Fermentation of L-Arabinose
( Suji Ye ),( Jeong-won Kim ),( Soo Rin Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
L-Arabinose, a five carbon sugar, has not been considered as an important bioresource because most studies have focused on D-xylose, another type of five-carbon sugar that is prevalent as a monomeric structure of hemicellulose. In fact, L-arabinose is also an important monomer of hemicellulose, but its content is much more significant in pectin (3-22%, g/g pectin), which is considered an alternative biomass due to its low lignin content and mass production as juiceprocessing waste. This review presents native and engineered microorganisms that can ferment L-arabinose. Saccharomyces cerevisiae is highlighted as the most preferred engineering host for expressing a heterologous arabinose pathway for producing ethanol. Because metabolic engineering efforts have been limited so far, with this review as momentum, more attention to research is needed on the fermentation of L-arabinose as well as the utilization of pectin-rich biomass.
Suji Han,Hyemi Shin,Jin-Ku Lee,Zhaoqi Liu,Raul Rabadan,Jeongwu Lee,Jihye Shin,Cheolju Lee,Heekyoung Yang,Donggeon Kim,Sung Heon Kim,Jooyeon Kim,Jeong-Woo Oh,Doo-Sik Kong,Jung-Il Le,Ho Jun Seol,Jung Wo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Glioblastoma (GBM) is the most lethal primary brain tumor with few treatment options. The survival of gliomainitiating cells (GICs) is one of the major factors contributing to treatment failure. GICs frequently produce and respond to their own growth factors that support cell proliferation and survival. In this study, we aimed to identify critical autocrine factors mediating GIC survival and to evaluate the anti-GBM effect of antagonizing these factors. Proteomic analysis was performed using conditioned media from two different patient-derived GBM tumor spheres under a growth factor-depleted status. Then, the antitumor effects of inhibiting an identified autocrine factor were evaluated by bioinformatic analysis and molecular validation. Proteins secreted by sphere-forming GICs promote cell proliferation/survival and detoxify reactive oxygen species (ROS). Among these proteins, we focused on midkine (MDK) as a clinically significant and pathologically relevant autocrine factor. Antagonizing MDK reduced the survival of GBM tumor spheres through the promotion of cell cycle arrest and the consequent apoptotic cell death caused by oxidative stress-induced DNA damage. We also identified PCBP4, a novel molecular predictor of resistance to anti-MDK treatment. Collectively, our results indicate that MDK inhibition is an important therapeutic option by suppressing GIC survival through the induction of ROS-mediated cell cycle arrest and apoptosis.
Suji Lee,Heon-Woong Kim,Ryeong Ha Kwon,So-Jeong Lee,Hyemin Na,Ju Hyung Kim,Chi-Do Wee,Seon Mi Yoo,Sang Hoon Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, a total of 93 flavonoid derivatives were identified and quantified from nine fermented soybean leaves (jangajji) with three different fermentation period using UPLC-DAD-QToF/MS on the basis of constructed flavonoid library. In positive ion mode, flavonoids derivatives were classified into fifty-seven flavonols, ten flavones and twenty-six isoflavones are mainly composed of acylated (malonyl and apiosyl) glycosides and mono, di, or tri glycosides based on aglycones including flavonol (quercetin, kaempferol, isorhamnetin), flavone (luteolin, apigenin, chrysoeriol), and isoflavone (daidzein, genistein, tectorigenin, afromosin, formononetin). As the fermentation period goes by, the contents of aglycones increased, however, the contents of mono, di, or triglycosides and acylated glycosides decreased. Overall, the total contents of flavonol, flavone, and isoflavone slightly increased on the fifth day of fermentation, but decreased in the subsequent period. From the results, this study can be a fundamental data for applying various processed soybean leaves, and the fermentation period of jangajji for the consumption of the desired amount of flavonoids can be considered.
Choi Suji,Park Jungmin,Jung Hyein,Jeong Jiwon,Lim Kyungjoon,Shin Sangah 한국영양학회 2024 Nutrition Research and Practice Vol.18 No.2
BACKGROUND/OBJECTIVES The prevalence of hypertension is increasing, and noodles have a high sodium content, so noodle consumption might be associated with a higher prevalence of hypertension. This study aimed to analyze the association between total and types of noodle intake and the risk of hypertension among Korean adults. Subjects aged 40–69 years were selected for this study. SUBJECTS/METHODS This study included 56,580 participants (18,246 men and 38,334 women) aged 40–69 years old from the Health Examinees study. Noodle and nutrient intakes were assessed using the food frequency questionnaire. Hypertension was diagnosed as systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg or antihypertensive medication use. Cox proportional hazard models were used to calculate the hazard ratio (HR) and 95% confidence interval (CI) for hypertension. RESULTS Higher noodle consumption was associated with increased hypertension among men (HR, 1.273; 95% CI, 1.166–1.390) and women (HR, 1.116; 95% CI, 1.038–1.199). CONCLUSION We recommended reducing noodle intake and consuming vegetables and fruits to increase potassium intake, which can prevent vascular diseases.