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RAPD Marker를 이용한 Zoysiagrass 수집계통과 종간교배 계통의 유전적 다양성 분석
金斗煥,毛淑淵,林星希 建國大學校 附設 農業資源開發硏究所 1997 農資源開發論集 Vol.19 No.-
ABSTRACT: RAPD markers were analyzed with zoysiagrass accessions and their hybrid lines in order to detect the genetic variation, and the following results were obtained.1. CTAB extraction method produced more and purer DNA than SDS method. RAPD reaction conditions were optimized as follows: 10ng template DNA, 270nM primer, 200μM each of dATP, dGTP and dTTP, 0.5μ unit dynazyme and 10x buffer brought to 15μl final volume with distilled water. The adequate annealing temperature was 36℃ and time was 30sec. Fifty cycles of amplification produced the best RAPD band patterns.2. Electrophoresis of PCR-products produced more polymorphic bands with polyacrylamide gel than with agarose gel. 3.The grouping patterns of zoysiagrass lines at dendrograms by RAPD markers were very different from the expected grouping pattern, and that was probably because of natural interspecific crossings for the long period. Therefore, the classification of the species within Zoysia genus will not be any longer meaningful and zoysiagrasses should be classified by the cultivar names or morphological characteristics such as leaf width.4. The zoysiagrass lines collected from Japan and Korea were belonged to the completely different groups at the dendrograms. The reason is expected to be the genetic drift due to geographical isolation.
고추재배 시 피복작물에 의한 피복율 변화와 잡초발생 양상
조정래(Jung-Lai Cho),최현석(Hyun-Sug Choi),이연(Youn Lee),이병모(Byung-Mo Lee),안난희(Nan-Hee An),박광래(Kwang-Lai Park),이지현(Ji-Hyun Lee) 韓國雜草學會 2011 Weed&Turfgrass Science Vol.31 No.4
나비나물속 주요 잡초인 얼치기완두와 새완두를 이용하여 고추 피복재배 기술을 개발하기 위해서 2008년과 2009년에 수행되었다. 두 초종 모두 2006년 6월에 충청남도 당진군에서 채종되어 2년 이상 상온에서 보관하였다. 피복재배 시 주요 월동 잡초는 냉이이고 주요 하절기 잡초는 돌피, 바랭이, 닭의장풀 이었다. 월동 우점잡초 냉이는 고추의 생육과 수량에 영향이 거의 없었다. 얼치기완두와 새완두 피복재배로 고추재배후기까지 피복도가 유지되었고 잡초발생을 현저하게 억제 하였다. 이에 따라 피복재배에 의해 고추 수량은 무처리구와 비교해서 통계적으로 유의성 있게 높았고, 관행재배에 비해서는 비슷하게 나타났다. This study was conducted to evaluate the effects of seeding of Vicia tetrasperma and V. hirsuta on the rate of ground cover as well as growth and yield of organic pepper plants in 2008 and 2009. All seeds of both species were gathered from Dangjin, Chungnam in late June of 2006 and stored for two year at room temperature. Capsella bursapastoris, Echinoloa crus-galli, Digitaria ciliaris, and Commelina communiswas were observed as main weeds during the winter and summer, respectively, at the field of V. tetrasperma and V. hirsuta. C. bursapastoris did not affect growth and yield of pepper plants. Seeding of V. tetrasperma and V. hirsuta maintained high percentage of ground cover later in the growing season of pepper plants and reduced significant amounts of weed in 2009. V. tetrasperma and V. hirsuta, therefore, produced more pepper yield compared to that of control but similar yield to the conventional system (polyethylene film).