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- 저자 : Steve Ow (검색결과 2 건)
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Selvarasu, Suresh,Ow, Dave Siak-Wei,Lee, Sang Yup,Lee, May May,Oh, Steve Kah-Weng,Karimi, Iftekhar A.,Lee, Dong-Yup Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and Bioengineering Vol.102 No.3
<P>Genome-scale flux analysis of Escherichia coli DH5α growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in the complex medium as well as the complete consumption of key carbohydrate nutrients, glucose and trehalose. Based on the consumption and production rates of the measured metabolites, constraints-based flux analysis of a genome-scale E. coli model was then conducted to elucidate their utilization in the metabolism. The in silico analysis revealed that the cell exploited biosynthetic precursors taken up directly from the complex medium, through growth-related anabolic pathways. This suggests that the cell could be functioning in an energetically more efficient manner by reducing the energy needed to produce amino acids. The in silico simulation also allowed us to explain the observed rapid consumption of serine: excessively consumed external serine from the complex medium was mainly converted into pyruvate and glycine, which in turn, led to the acetate accumulation. The present work demonstrates the application of an in silico modeling approach to characterizing microbial metabolism under complex medium condition. This work further illustrates the use of in silico genome-scale analysis for developing better strategies related to improving microbial growth and enhancing the productivity of desirable metabolites. Biotechnol. Bioeng. 2009; 102: 923–934. © 2008 Wiley Periodicals, Inc.</P>
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High School Students' Perceived Creativity Self-efficacy and Emotions in a Service Learning Context
Ai-Girl Tan,Valerie Ho,Elizabeth Ho,Steve Ow 대한사고개발학회 2008 The International Journal of Creativity & Problem Vol.18 No.2
Our study examined the relationship of creativity self-efficacy and emotions of high school students (n = 279) in Singapore before and after they attended a three to five-day international service learning program. Participants completed a creativity self efficacy scale, the Positive Affect and Negative Affect Scale (PANAS), Satisfaction With Life Scale (SWLS) and Subjective Happiness Scale (SHS). We found a positive, significant correlation between creativity efficacy and positive affect, life satisfaction and subjective happiness but negative correlation with negative mood affect. In addition, students whose creativity efficacy was moderately high were found to have reported higher ratings of life satisfaction after having participated in service learning. Our findings provide initial evidence suggesting that service learning with positive intervention is positively related to creativity self-efficacy and happiness of youth.
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