재사용성을 고려한 데이터기록분석도구 설계 방안 연구

김성민 ( Soungmin-kim ),이용헌 ( Yong-heon Lee ),조규태 ( Kyu-tae Cho ),이승영 ( Seung-young Lee ) 한국정보처리학회 2016 한국정보처리학회 학술대회논문집 Vol.23 No.2

무기체계가 고도화되고 데이터의 양이 증가하는 현재 체계 개발에서 데이터기록분석도구는 오류 검출 및 성능 측정을 위해 필수적인 장비이다. 하지만 데이터기록분석도구가 네트워크 프로토콜 및 데이터 구조에 종속되어 있기 때문에 재사용이 불가능한 단점을 지니고 있다. 본 논문에서는 재사용성을 고려하여 데이터에 독립적이고 네트워크 프로토콜 추가 및 변경이 용이한 구조를 갖는 데이터기록분석도구 설계 방안을 제안하고자 한다.

혀뿌리 신경집종과 동시 발생한 중이 칼시노이드 환자 1례

이민형(Min Hyeong Lee),이승민(Soungmin Lee),김상연(Sang-Yeon Kim),전범조(Beom Cho Jun) 대한두개저학회 2019 대한두개저학회지 Vol.14 No.2

Carcinoid tumors are neuroendocrine tumors which arise from the enterochromaffin cells; the neoplasms grow slowly and are usually found in the gastrointestinal and bronchopulmonary systems. Carcinoid tumors in the middle ear are uncommon since the neuroendocrine cells have not been identified in middle ear mucosa. In this case, a 46-year-old man had tinnitus in right ear for a year. From otoendoscopy findings, we observed a complete perforation of right tympanic membrane and a pinkish mass like lesion at the center of the perforated tympanic membrane. Canal wall up mastoidectomy and tympanoplasty were done, and pathological findings showed cuboidal or columnar cells with small, round, and uniform nuclei; immunohistochemistry demonstrated positive for CD 56 but negative for S-100. Ki- 67 proliferation activity was low. While the patient underwent intubation for general anesthesia, a 2 cm polypoid mass was found at right base of tongue. The mass was removed by robotassisted surgery, and the pathology demonstrated neurilemmoma. The aut

Removal and Inactivation of Hepatitis A Virus during Manufacture of a High Purity Antihemophilic Factor VIII Concentrate from Human Plasma

Kim, In-Seop,Park, Yong-Woon,Lee, Sung-Rae,Lee, Mahl-Soon,Huh, Ki-Ho,Lee, Soungmin The Microbiological Society of Korea 2001 The journal of microbiology Vol.39 No.1

A validation study was conducted to evaluate the efficacy and mechanism of the cryo-precipitation, monoclonal anti-FVIIIc antibody (mAb) chromatography, Q-Sepharose chromatography, and lyophilization steps involved in the manufacture of high purity factor VIII (GreenMono) from human plasma, in the removal and/or inactivation of hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and subjected to scale-down processes mimicking the manufacture of the high purity factor VIII concentrate. Samples were collected at each step and immediately titrated using a 50% tissue culture infectious dose (TCID$\_$50/) and then the virus reduction factors were evaluated. HAV was effectively partitioned from factor VⅢ during cryo-precipitation with the log reduction factor of 3.2. The mAb chromatography was the most effective step far removal of HAV with the log reduction factor of $\geq$4.3. HAV infectivity was not detected in the fraction of factor VⅢ, while most of HAV infectivity was recovered in the fractions of flow through and wash during mAb chromatography. Q-Sepharose chromatography showed the lowest efficacy for partitioning HAV with the log reduction factor of 0.7. Lyophilization was an effective step in inactivating HAV with the log reduction factor of 2.3. The cumulative lag reduction factor, $\geq$10.5, achieved for tile entire manufacturing process was several magnitudes greater than the potential HAV load of current plasma pools.

Removal and Inactivation of Hepatitis A Virus during Manufacture of Urokinase from Human Urine

In Seop Kim,Yong Woon Choi,Sung Rae Lee,Yong Kang,Kyung Myung Lee,Dae Han Park,Han Sang Woo,Soungmin Lee 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.6

The purpose of the present study was to examine the efficacy and mechanism of thePAB (para-amino benzamidine) affinity column chromatography, Viresolve NFP virus filtration, pasteurization (60oC heat treatment for 10 h), and lyophilization steps employed in the manufacture of urokinase from human urine as regards the removal and/or inactivation of the hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and subjected to scale-down processes mimicking the manufacture of urokinase. Samples were colected at each step, immediately titrated using a 50. tissue culture infectious dose (TCID50), and the virus reduction factors evaluated. PAB chromatography was found to be an effective step for removing HAV with a log reduction factor of 3.24. HAV infectivity was rarely detected in the urokinase fraction, while most of the HAV infectivity was recovered in the unbound and wash fractions. HAV was completely removed during the Viresolve NFP filtration with a log reduction factor of .the titers were reduced from an initial titer of 7.18 log10 TCID50 to undetectable levels within 10 h of treatment. The log reduction factor achieved during pasteurization was . 4.76. Lyophilization revealed the lowest efficacy for inactivating HAV with a log reduction factor of 1.48. The cumulative log reduction factor was . 14.08. Acordingly, these results indicate that the production process for urokinase exhibited a sufficient HAV reducing capacity to achieve a high margin of virus safety. Keywords

Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve

Seo, NaRi,Lee, Sung-Ho,Ju, Kyung Won,Woo, JaeMan,Kim, BongJu,Kim, SoungMin,Jahng, Jeong Won,Lee, Jong-Ho Medknow PublicationsMedia Pvt Ltd 2018 Neural regeneration research Vol.13 No.1

<P>Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field (PEMF) reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using <I>in vitro</I> and <I>in vivo</I> experiments. In <I>in vitro</I> experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 (Schwann cell marker), glial fibrillary acidic protein (astrocyte marker), and brain-derived neurotrophic factor and nerve growth factor (neurotrophic factors) mRNA expression. In the <I>in vivo</I> experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site (1 × 10<SUP>6</SUP> cells). DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. <I>In vitro</I> experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. <I>In vivo</I> experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.</P>

Perceived service quality and its influence on behavioral intention in South Korean public dental hospitals.

Kim, Yukyoung,Kim, Soungmin,Myoung, Hoon,Lee, Hyung Ryong Science Press 2012 Asia-Pacific journal of public health Vol.24 No.2

<P>South Korean national university dental hospitals (NUDHs) face unprecedented challenges in maintaining primary function as public hospitals and surviving in intensified competition. The aim of the study was to evaluate the perceived service quality of NUDH patients and its influences on behavior and to gain managerial implications. Perceived service quality, value, satisfaction, and behavioral intention were measured in 438 NUDH patients from 3 NUDHs. With demographic analyses, the authors used structural equation models to test the validity to prove the relationship between dimensions.</P>

노랑털깔따구(Chironomus flaviplumus) 성충의 알레르기 항원단백 분석

이한일(Han-Il REE),이상화(Sang-Hwa LEE),김유경(Yu-Kyoung KIM),전승민(Soungmin JEON),장재경(Jae-Kyung CHANG),김윤석(Yoon-Suk KIM) 대한기생충학열대의학회 1996 The Korean Journal of Parasitology Vol.34 No.1

Partitioning and Inactivation of Viruses by Cold Ethanol Fractionation and Pasteurization during Manufacture of Albumin from Human Plasma

KIM, IN SEOP,EO, HO GUEON,CHANG, CHONG EUN,LEE, SOUNGMIN 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.6

The purpose of the present study was to examine the efficacy and mechanism of the fraction Ⅳ cold ethanol fractionation and pasteurization (60℃ heat treatment for 10h) steps, involved in the manufacture of albumin from human plasma, in the removal and/or inactivation of blood-born viruses. A variety of experimental model viruses for human pathogenic viruses, including the Bovine viral diarrhoea virus (BVDV), Bovine herpes virus (BHV), Murine encephalomyocarditis virus (EMCV), and Porcine parvovirus (PPV), were selected for this study. Samples from the relevant stages of the production process were spiked with the viruses, and the amount of virus in each fraction was then quantified using a 50% tissue culture infectious dose (TCID_50). The mechanism of reduction for the enveloped viruses (BHV and BVDV) during fraction Ⅳ fractionation was inactivation rather than partitioning, however, it was partitioning in the case of the non-enveloped viruses (EMCV and PPV). The log reduction factors achieved during fraction Ⅳ fractionation were ≥6.9 for BHV, ≥5.2 for BVDV, 4.9 for EMC, and 4.0 for PPV. Pasteurization was found to be a robust and effective step in inactivating the enveloped viruses as well as EMCV. The log reduction factors achieved during pasteurization were ≥7.0 for BHV, ≥6.1 for BVDV, ≥6.3 for EMCV, and 1.7 for PPV. These results indicate that the production process for albumin has sufficient virusreducing capacity to achieve a high margin of virus safety.