Comparative Genomic Analysis of Staphylococcus aureus FORC_001 and S. aureus MRSA252 Reveals the Characteristics of Antibiotic Resistance and Virulence Factors for Human Infection

( Sooyeon Lim ),( Dong Hoon Lee ),( Woori Kwak ),( Hakdong Shin ),( Hye Jin Ku ),( Jong Eun Lee ),( Gun Eui Lee ),( Heebal Kim ),( Sang Ho Choi ),( Sangryeol Ryu ),( Ju Hoon Lee ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1

Staphylococcus aureus is an important foodborne pathogen that causes diverse diseases ranging from minor infections to life-threatening conditions in humans and animals. To further understand its pathogenesis, the genome of the strain S. aureus FORC_001 was isolated from a contaminated food. Its genome consists of 2,886,017 bp double-stranded DNA with a GC content of 32.8%. It is predicted to contain 2,728 open reading frames, 57 tRNAs, and 6 rRNA operons, including 1 additional 5S rRNA gene. Comparative phylogenetic tree analysis of 40 complete S. aureus genome sequences using average nucleotide identity (ANI) revealed that strain FORC_001 belonged to Group I. The closest phylogenetic match was S. aureus MRSA252, according to a whole-genome ANI (99.87%), suggesting that they might share a common ancestor. Comparative genome analysis of FORC_001 and MRSA252 revealed two non-homologous regions: Regions I and II. The presence of various antibiotic resistance genes, including the SCCmec cluster in Region I of MRSA252, suggests that this strain might have acquired the SCCmec cluster to adapt to specific environments containing methicillin. Region II of both genomes contains prophage regions but their DNA sequence identity is very low, suggesting that the prophages might differ. This is the first report of the complete genome sequence of S. aureus isolated from a real foodborne outbreak in South Korea. This report would be helpful to extend our understanding about the genome, general characteristics, and virulence factors of S. aureus for further studies of pathogenesis, rapid detection, and epidemiological investigation in foodborne outbreak.

THE EFFECT OF THE ATTRIBUTES OF IT SHOPPING SERVICE AND THE LEVEL OF TECHNOLOGY ON CUSTOMER RESPONSES IN FASHION RETAIL

Sooyeon Lee,Miah Lee,Eunju Ko,Sangwon Lee 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.7

According to the development of information technology (IT) and new media, customers’ needs have changed to seek not only high quality goods but also a differentiated service in retail stores (Lee & Shin, 2011). For this reason, fashion retail stores started to provide an interesting service by setting up an IT machine or a gadget in the stores. Thereby, customers can get the information or have a special experience while they shop fashion goods. IT shopping service is defined as using technology in retail stores to enhance interaction between retailers and customers. Retailers have tried several types of IT shopping services, which offer information or experience with a high or low level of technology. Informative IT shopping service provides various and useful information, which is related to each product such as size, color, contents, stock, location and fashion styling. Retailers use experiential IT shopping service as an emotional appeal that gives direct experience to customers in some way or other such as watching or acting in a virtual situation. Also, as technology develops, IT shopping services are embodied with diverse technology that are simple or complexity. Some are easy to use with one button and shows 2D images only, but some have a lot of contents such as video clips, SNS share service, showing 3D images, and implementing augment reality. We saw customer responses on the attributes of IT shopping service (informative vs. experimental) x technology level of IT shopping service (high vs. low) x fashion innovativeness/technology innovativeness (high vs. low) This study aims to see the effect of the attributes of IT shopping service and the level of technology on procedure satisfaction and outcome satisfaction, and the moderating effect of perceived enjoyment and perceived complexity. Furthermore, this study will see whether these effects affect visit intention. In this study, the experimental design was adopted as the main methodology, and a total of four stimuli types were selected: 1) informative x high technology 2) informative x low technology 3) experiential x high technology 4) experiential x low technology. The common element, which all types of stimuli have, is a digital touch screen since it is a general equipment nowadays, and the common fashion good for all types is a black blazer. The type of informative service only has the information (size range, color assortment, contents, stock) about selected fashion good with its image. High technology in informative service shows the image with 3D simulation that can help to see 360° of the blazer, and styling tips including a video clip. Moreover, it helps to share the information with friends via SNS. Low technology in informative service only shows a front side of the blazer and presents a store map for customers to find a product they want to try. The types of experiential service draw customer participation providing a picture of customers that are trying on clothes. Experiential service use augment reality (AR) as a high technology and take the back side of customers picture as low technology. For the manipulation check, 18 graduate students in total viewed for each type, and the total sample comprises 387 college students via survey instrument. All hypotheses were tested using AMOS and SPSS 18.0. The main findings of this study are as follows: depending on type of the attributes of IT shopping service and the level of technology affect perceived enjoyment and perceived complexity differently, and there were significant mediating effect of perceived variables toward IT shopping service on procedure satisfaction, outcome satisfaction, and visit intention. Implications for usefulness and role of the IT shopping service in fashion retail stores are provided. And also, limitation of this study and future study are suggested.

THE EFFECT OF THE ATTRIBUTES OF IT SHOPPING SERVICE AND THE LEVEL OF TECHNOLOGY ON CUSTOMER RESPONSES IN FASHION RETAIL

Sooyeon Lee,Miah Lee,Eunju Ko,Sangwon Lee 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.6

According to the development of information technology (IT) and new media, customers’ needs have changed to seek not only high quality goods but also a differentiated service in retail stores (Lee & Shin, 2011). For this reason, fashion retail stores started to provide an interesting service by setting up an IT machine or a gadget in the stores. Thereby, customers can get the information or have a special experience while they shop fashion goods. IT shopping service is defined as using technology in retail stores to enhance interaction between retailers and customers. Retailers have tried several types of IT shopping services, which offer information or experience with a high or low level of technology. Informative IT shopping service provides various and useful information, which is related to each product such as size, color, contents, stock, location and fashion styling. Retailers use experiential IT shopping service as an emotional appeal that gives direct experience to customers in some way or other such as watching or acting in a virtual situation. Also, as technology develops, IT shopping services are embodied with diverse technology that are simple or complexity. Some are easy to use with one button and shows 2D images only, but some have a lot of contents such as video clips, SNS share service, showing 3D images, and implementing augment reality. We saw customer responses on the attributes of IT shopping service (informative vs. experimental) x technology level of IT shopping service (high vs. low) x fashion innovativeness/technology innovativeness (high vs. low) This study aims to see the effect of the attributes of IT shopping service and the level of technology on procedure satisfaction and outcome satisfaction, and the moderating effect of perceived enjoyment and perceived complexity. Furthermore, this study will see whether these effects affect visit intention. In this study, the experimental design was adopted as the main methodology, and a total of four stimuli types were selected: 1) informative x high technology 2) informative x low technology 3) experiential x high technology 4) experiential x low technology. The common element, which all types of stimuli have, is a digital touch screen since it is a general equipment nowadays, and the common fashion good for all types is a black blazer. The type of informative service only has the information (size range, color assortment, contents, stock) about selected fashion good with its image. High technology in informative service shows the image with 3D simulation that can help to see 360º of the blazer, and styling tips including a video clip. Moreover, it helps to share the information with friends via SNS. Low technology in informative service only shows a front side of the blazer and presents a store map for customers to find a product they want to try. The types of experiential service draw customer participation providing a picture of customers that are trying on clothes. Experiential service use augment reality (AR) as a high technology and take the back side of customers picture as low technology. For the manipulation check, 18 graduate students in total viewed for each type, and the total sample comprises 387 college students via survey instrument. All hypotheses were tested using AMOS and SPSS 18.0. The main findings of this study are as follows: depending on type of the attributes of IT shopping service and the level of technology affect perceived enjoyment and perceived complexity differently, and there were significant mediating effect of perceived variables toward IT shopping service on procedure satisfaction, outcome satisfaction, and visit intention. Implications for usefulness and role of the IT shopping service in fashion retail stores are provided. And also, limitation of this study and future study are suggested.

고농도 에틸렌 노출에 의한 참다래의 후숙 단축 연구

임수연(Sooyeon Lim),이정용(Jeong-Yong Lee),서성권(Sung-Gown Seo),이정구(Jung Gu Lee),이한준(Han Jun Lee),한승현(Seung Hyun Han),이은진(Eun Jin Lee) 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5

Extensive congenital verrucous venous malformation accompanied with a cerebral cavernous malformation in a neonate with PDCD10 deletion: a first case report

( Joo Hee Lee ),( Sooyeon Choi ),( Jemin Kim ),( Young In Lee ),( Jihee Kim ),( Soo-chan Kim ),( Sang Eun Lee ),( Ju Hee Lee ) 대한피부과학회 2022 대한피부과학회 학술발표대회집 Vol.73 No.2

Classification and Ordination of Ground Beetle (Coleoptera: Carabidae) Assemblage Among Habitat Types in Jeonju, Korea

Jongkook Jung,Seungtae Kim,Sooyeon Lee,Hunsung Kim,Myeong-Pyo Jung,Sejin Kim,Chang-Kyu Park,Eunhee Lee,Joon-Ho Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

This study was conducted to explore if the ground beetle (Coleoptera: Carabidae) can be used as an indicator classifying habitat types. Thirteen land use types were selected as survey sites in Jeonju. Ground beetles were collected by 3 pitfall traps (15 cm diameter) for each site from June 20 to September 22, 2008. Pitfall traps were replaced at one month interval. Total 919 ground beetles of 31 species belonging to 17 genera were collected. Land use types were classified and ordinated by two-way indicator species analysis and detrended correspondence analysis. Land use types were classified and ordinated into two major groups, forest and non-forest, by Synuchus nitidus and Dolichus halensis. Two major groups were subdivided into 4 end groups; forest, riverside, upland and other sites. Other sites group including 4 sites; levee, public garden, outfield and manufacturing area were not coincided with land use types. Nevertheless, ground beetles appear to be used as indicators of habitat types.

Major Metabolites Involved in Skin Blackening of ‘Nittaka’ Pear Stored under Cold Temperature

Sooyeon Lim,Seung Hyun Han,Han Jun Lee,Jeong Koo Lee,Eun Jin Lee 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5

Inhibition of Hardy Kiwifruit (Actinidia Aruguta) Ripening by 1-methylcyclopropene During Cold Storage and Anticancer Properties of the Fruit Extract

Sooyeon Lim,Eun Jin Lee,Jeong Gu Lee,Jee won Bang,Joonggon Kim 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.10

Gene-Related Strain Variation of Staphylococcus aureus for Homologous Resistance Response to Acid Stress

LEE, SOOMIN,AHN, SOOYEON,LEE, HEEYOUNG,KIM, WON-IL,KIM, HWANG-YONG,RYU, JAE-GEE,KIM, SE-RI,CHOI, KYOUNG-HEE,YOON, YOHAN International Association for Food Protection 2014 Journal of food protection Vol.77 No.10

<P>This study investigated the effect of adaptation of Staphylococcus aureus strains to the acidic condition of tomato in response to environmental stresses, such as heat and acid. S. aureus ATCC 13565, ATCC 14458, ATCC 23235, ATCC 27664, and NCCP10826 habituated in tomato extract at 35°C for 24 h were inoculated in tryptic soy broth. The culture suspensions were then subjected to heat challenge or acid challenge at 60°C and pH 3.0, respectively, for 60 min. In addition, transcriptional analysis using quantitative real-time PCR was performed to evaluate the expression level of acid-shock genes, such as clpB, zwf, nuoF, and gnd, from five S. aureus strains after the acid habituation of strains in tomato at 35°C for 15 min and 60 min in comparison with that of the nonhabituated strains. In comparison with the nonhabituated strains, the five tomato-habituated S. aureus strains did not show cross protection to heat, but tomato-habituated S. aureus ATCC 23235 showed acid resistance. In quantitative real-time-PCR analysis, the relative expression levels of acid-shock genes (clpB, zwf, nuoF, and gnd) were increased the most in S. aureus ATCC 23235 after 60 min of tomato habituation, but there was little difference in the expression levels among the five S. aureus strains after 15 min of tomato habituation. These results indicate that the variation of acid resistance of S. aureus is related to the expression of acid-shock genes during acid habituation.</P>

Correlation Between Glucosinolates Profiles and Individual Amino Acids in Brassica Species Seeds

Sooyeon Lim,Jeong Gu Lee,Jongkee Kim,Eun Jin Lee 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.5