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안순영(Soonyoung Ahn),김기덕(Ki Deog Kim),이종남(Jong Nam Lee),임주성(Ju Sung Im),남춘우(Chun Woo Nam),정진철(Jin Chel Jung),이응호(Eung Ho Lee) 한국원예학회 2008 원예과학기술지 Vol.26 No.4
고랭지배추 내 잔류농약을 제거하기 위하여 일반가정에서 많이 사용하는 4가지 세척제(수돗물, 베이킹소다(0.2%), 숯을 하루 담가둔 물(20g・L<SUP>-1</SUP>, pH 7.2), 식초(5%))를 이용하여 세척제에 의한 잔류농약 저감률을 알아보았다. 세척제에 따른 잔류농약의 저감률은 수돗물은 26.5-83.8%, 베이킹소다(0.2%) 사용 시 32.7-93.3%, 숯을 하루 담가둔 물(20g・L<SUP>-1</SUP>,pH 7.2)은 40.3-89.7%, 식초(5%) 사용 시 22.9-85.1% 사이로 나타났다. 잔류농약 제거율은 농약별로는 bifenthrin, kresoxim-methyl, pyraclofos, metalaxyl 순으로 높게 나타났다. 세척제에 따른 잔류농약 평균 제거율은 숯을 하루 담가둔 물(20g・L<SUP>-1</SUP>, pH 7.2), 베이킹소다(0.2%), 수돗물, 식초(5%) 순이며, 60% 정도의 평균 제거율을 나타내었다. 세척제에 의한 농약의 제거율은 각 농약의 수용성과는 상관관계가 없었다. This study was conducted to examine the removal efficiency of 4 pesticides in Chinese cabbage (Brassica campestris L.) by different types of washing solution after harvest. To determine the removal efficiency of different washing solutions, samples were stored at 4℃, washed by different washing solutions and analyzed for amount of pesticide residues in Chinese cabbage. Removal efficiency of pesticide by tap water, baking soda (0.2%), charcoal water (20 g・L<SUP>-1</SUP>, pH 7.2), vinegar (5%) ranged from 26.5-83.8%, 32.7-93.3%, 40.3-89.7%, and 22.9-85.1%, respectively. The order of average removal efficiency of the pesticides was bifenthrin (82.7%), kresoxim-methyl (79.7%), pyraclofos (44.4%) and metalaxyl (38.2%). The washing efficiency of a pesticide is not correlated with its water solubility.
RAPD 및 ITS 염기서열 분석을 이용한 곰취 속(Ligularia) 식물의 유연관계 분석
안순영(Soonyoung Ahn),조광수(Kwang-Soo Cho),유기억(Ki-Oug Yoo),서종택(Jong-Taek Suh) 한국원예학회 2010 원예과학기술지 Vol.28 No.4
RAPD와 ITS 염기서열 분석을 통하여 Ligularia 속 식물5종류의 유연관계를 밝혔다. RAPD 분석에서는 총 196개의 random primer를 사용하여 밴드수가 많고 선명한 63개의 primer를 선발하였다. 다형성을 나타낸 밴드는 141개(31.8%)이었으며, 증폭된 크기는 0.2-1.6kb로 다양하였다. 유집 분석결과, 유사도 값은 0.54-0.95의 범위로 나타났고, 0.77을 기준으로 크게 5그룹으로 나누었다. ITS 영역의 염기서열 분석결과, ITS 1과 ITS 2 지역은 각각 248-256bp와, 220-222bp로 구성되어 있으며, 5.8S 부분은 164bp로 나타났다. ITS 1과 ITS 2 지역의 총 478개의 염기 중 49(10.2%)군데에서 변이가 있었으며, 구아닌(G)과 시토신(C)의 비율은 ITS 1 지역에서 49.4%, ITS 2에서는 53.5%로 나타났다. 염기서열분석결과 5종류는 단계통을 형성하였으며, 갯취는 군외군으로 부터 가장 먼저 분계조를 형성하였다. 한대리곰취와 어리곤달비는 79%의 지지율을 가지고 유집되었으며, 곰취와 곤달비도 함께 유집되었지만 지지도는 52%로 낮았다. 이상의 결과에서 두 데이터는 일치하는 결과를 보였지만 한대리곰취의 분류학적 위치는 RAPD와 ITS 분석결과가 일치하지 않았다. The genetic relationships in 5 species of Ligularia were investigated using RAPD (Randomly Amplified Polymorphic DNA) and ITS (Internal Transcribed Spacer) sequences analyses. In RAPD analysis, sixty three of 196 arbitrary primers showed polymorphism. The amplified fragments ranged from 0.2 to 1.6 kb in size. The dendrogram was constructed by the UPGMA clustering algorithm based on genetic similarity of RAPD markers. A total of 16 accessions were classified into 5 major groups corresponding each species at the similarity coefficient value of 0.77. In the ITS sequence analysis, the size of ITS 1 was varied from 248 to 256 bp, while ITS 2 was varied from 220 to 222 bp. The 5.8S coding region was 164 bp in lengths. Forty nine sites (10.2%) of the 478 nucleotides were variable, and the G+C content of ITS region ranged from 49.4 to 53.5%. In the ITS tree, five species of Ligularia were monophyletic, and L. taquetii was the first branching within the clade. Ligularia intermedia formed a clade with L. fischeri var. spiciformis (BS=79), and L. stenocephala and L. fischeri were also claded. Two data sets were congruent, except of the position of L. fischeri var. spiciformis.
형질전환 토마토 후대 분리세대에 활용할 수 있는 비파괴적 제초제 생물검정법 개발
안순영(Soonyoung Ahn),장인옥(Inok Jang) 한국원예학회 2007 원예과학기술지 Vol.25 No.3
제초제 저항성 PAT 유전자가 도입된 형질전환 토마토의 후대 분리 세대에서 제초제 저항성을 빠르고 간편하게 확인 할 수 있는 비파괴 제초제 생물검정 방법을 확립하였다. 형 질전환체와 비 형질전환체 후대 분리 집단에서 잎을 채취하여 5가지 농도의 바스타 용액에서 제초제에 대한 저항성 및 감수성을 판별할 수 있는 적정 농도를 검토한 결과 25㎎ ℓ⁻¹가 대량 비파괴 제초제 생물검정에 적정한 것으로 나타났다. 대량 비파괴 제초제 생물검정법은 형질전환체의 후대 분리 집단에서 채취한 잎을 25㎎ ℓ⁻¹ 농도의 바스타 용액이 담긴 아크릴 박스(50×50㎝)에 순서대로 두고 밀봉한 후 5일 후에 제초제에 대한 저항성을 확인하였는 바, 제초제에 대한 저항성과 감수성의 구분이 뚜렷하였으며, 이 방법을 이용하여 대량의 분리 집단에서 형질전환체를 빠르고 간편하고 선별할 수 있을 것으로 생각되었다. This study was conducted to develop a non-destructive herbicide bioassay method to confirm the transgenic tomatoes in segregation populations. In order to find the proper concentration of bialaphos solution that discriminates resistance and susceptible, the filter papers were soaked in different concentrations of bialaphos solution (10, 25, 50, 75, and 100 ㎎ ℓ⁻¹). The proper concentration of bialaphos solution was found out to be 25 ㎎ ℓ⁻¹. One hundred leaflets were placed in acryl boxes moistened with filter papers that were soaked in a bialaphos solution (25 ㎎ ℓ⁻¹) for the screening of herbicide resistance. In addition, to determine the effect of leaf stage of seedlings, at the one, three, and five true-leaf stage were separately tested in the same manner. In the segregating population, non-destructive method results were matched exactly with 0.3% bialaphos application in a greenhouse.
육군 야전부대 의무요원의 병원 전 단계 외상처치 지식 및 수행능력, 교육요구, 경험에 관한 연구
이순영 ( Soonyoung Lee ),안종성 ( Jongseong Ahn ),김영희 ( Young Hee Kim ),이성배 ( Sung Bae Lee ) 국군의무사령부 2015 대한군진의학학술지 Vol.46 No.1
Objectives; This Study was to examine pre-hospital trauma care knowledge, Performance, education need, and experiences of the healthcare providers working at field units Methods; A total of 226 healthcare providers belonging to 1st Army field units completed self-reported questionnaire of pre-hospital trauma care knowledge, performance, educational need and experience from December 1, 2014 to March 30, 2015. Results; Seventy nine(43.4%) and 61(27.0%)healthcare providers had experience of trauma care and casualty transfer respectively. One hundred and thirty two(58.4%) healthcare providers experienced trauma care education programs. The overall mean scores of trauma care perceived by the participants were knowledge(4.11±.70), performance level(4.04±.71), and educational needs(3.39±1.24). Knowledge level in trauma care perceived by the participants positively correlated individual ability, units' ability, and performance level. Conclusions; Based on the results of this study, it is necessary to develop trauma care training program which can promote the competency of pre-hospital trauma care and casualty transfer for healthcare providers working at field units.
Ternary Content Addressable Memory를 위한 저 전력 Rail-to-Rail 감지 증폭기
안상욱(Sangwook Ahn),정창민(Changmin Jung),임철승(Chulseung Lim),이순영(Soonyoung Lee),백상현(Sanghyeon Baeg) 大韓電子工學會 2012 電子工學會論文誌-SD (Semiconductor and devices) Vol.49 No.2
The newly designed sense amplifier in this paper has rail-to-rail input range achieving low power consumption. Reducing static power consumption generated due to DC path to ground is key element for low power consumption in this paper. The proposed sense amplifier performs power-saving operation using negative feedback circuit that controls the current flow with the newly added PMOS input terminal. As a simulation result, the proposed sense amplifier consumed about over 50 % efficiency of the average power consumed by the typical Rail-to-Rail sense amplifier.
Kinase Suppressor of Ras 1 Promotes YAP/TAZ-Mediated Tumorigenesis in the Liver
( Hyuk Moon ),( Kyungjoo Cho ),( Soonyoung Shin ),( Simon W. Ro ),( Beom Kyung Kim ),( Seung Up Kim ),( Jun Yong Park ),( Sang Hoon Ahn ),( Do Young Kim ),( Kwang-hyub Han ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Liver cancer is the second most common cause of cancer-related deaths worldwide. The Hippo signaling pathway is tumor suppressive, as its inactivation leads to tissue overgrowth and tumor formation via YAP- or TAZ-mediated transcriptional activation. YAP is overexpressed in 62% of patients with hepatocellular carcinoma (HCC) and in almost 90% of human cholangiocarcinoma (CCC). Kinase Suppressor of Ras 1 (KSR1) is a scaffold protein for the Ras/Raf/MEK/ERK signaling pathway, promoting activation of MEK and ERK. Pro-tumorigenic roles of KSR1 in Ras-activated cancers have been verified in murine models for lung, skin, and pancreatic cancers. In this study, we have investigated the role of KSR1 in YAP/TAZ-mediated hepatocarcinogenesis. Methods: Transposons were constructed encoding KSR1 and an activated from of TAZ (TAZ<sup>S89A</sup>). Transposons were hydrodynamically delivered to livers of 6-week-old C57BL/6 mice. Mice were monitored at least twice per week and sacrificed when moribund. Tumor-bearing livers were formalin fixed for hematoxylin-eosin staining and immunohistochemistry. Results: Analysis of gene expression levels in human HCC and CCC samples deposited in The Cancer Genome Atlas (TCGA) revealed that KSR1 was significantly upregulated both in human HCC and CCC, compared with non-tumoral surrounding livers (P<0.0001 in HCC and P=0.0063 in CCC). Co-expression of KSR1 and an activated form of TAZ (TAZ<sup>S89A</sup>) led to the development of both HCC and CCC in the murine livers, while expression of TAZ<sup>S89A</sup> alone failed to induce hepatic tumors. Conclusions: KSR1 promotes hepatocarcinogenesis, both in HCC and CCC. Suppression of KSR1 might be an attractive therapeutic option for both types of hepatic malignancies.