Asobara japonica의 벗초파리 발육단계에 따른 기생특성 및 살충효과

윤승환,이진구,장재은,한정아,김상우,김아영,서홍렬,이영순,Yun, Seung-Hwan,Lee, Jingu,Jang, Jae Eun,Han, Jeong-A,Kim, Sang-Woo,Kim, A-Young,Seo, Hong-Yul,Lee, Young Soon 한국응용곤충학회 2021 한국응용곤충학회지 Vol.60 No.3

벗초파리기생벌인 A. japonica의 벗초파리 유충의 발육단계에 따른 기생특성과 기생당한 유충과 번데기에서 형태적인 차이를 조사하였다. 또한 A. japonica의 우화기간이 벗초파리 우화일수 보다 더 소요되는 것을 확인하였다. A. japonica는 벗초파리 유충에 효과적인 기생 및 살충 효과를 나타내었으며 벗초파리 방제를 위한 천적으로 활용이 가능할 것으로 보인다. We confirmed the parasitic characteristics of Asobara japonica according to the stage of development of Drosophila suzukii and observed the morphological differences between parasitized and unparasitized D. suzukii larvae and pupae. It was also confirmed that emergence period of A. japonica was longer than that of D. suzukii. A. japonica showed an effective parasitic and insecticidal effect on D. suzukii larvae, and it is expected that A. japonica could be used as a natural enemy for the control of D. suzukii.

일반화가능도 이론을 활용한 TTCT (도형 A형- 활동 2) 독창성 평가 방안 탐색

이진아(LEE JIN A),한기순(Han ki Soon) 한국창의력교육학회 2016 창의력교육연구 Vol.16 No.3

본 연구에서는 다양한 분산오차를 다각도로 산출해낼 수 있는 일반화가능도 이론을 활용하여 TTCT 도형 A검사 독창성 항목 평가의 최적화 방안을 탐색하는 데 그 목적이 있다. 이를 위하여 수도권 지역 대학생 273명을 대상으로 TTCT도형 A검사 활동2를 실시하여 정규형 버전과 간편형 버전으로 각각 채점을 하였다. 주요 결과를 요약하면 다음과 같다. 첫째, 두 가지 채점기준에 의해 살펴본 각 요인들의 상대적 영향력 분석 결과 두 채점 방식은 공통적으로 피험자와 문항의 상호작용에 의한 오차분산이 매우 큰 것으로 나타났으며, 채점자와 관련된 오차 분산은 매우 낮게 나타났다. 둘째, 채점 방법에 따른 최적의 측정조건 탐색 결과 두 채점기준 모두 채점자 수를 늘리는 것은 효과가 미미했으며, 문항수의 경우는 기존보다 훨씬 많은 문항이 필요하다는 결과값이 도출되었다. 즉, 연구의 편의와 수월성을 위해 TTCT도형 A검사 활동2만을 활용하여 측정할 경우 정규형, 간편형 채점 모두에서 독창성 측정에 있어 연구 결과의 왜곡을 초래할 가능성이 있음이 시사되었다. 더불어 향후 TTCT채점 방식의 최적화를 위해 좀 더 심층적이고 다각적인 노력이 필요함이 시사되었다. This research aims to find ways to optimize TTCT originality scoring system, using the generalizability theory to identify origin of errors in various perspectives. For this, TTCT Figure A (only section 2) had been carried out on 273 university students in metropolitan areas and scored separately with both standard and simplified versions. The results are as follows. First, the results by two separate evaluation guidelines have indicated that both guidelines had shown significantly high error variance between its items and the subjects, whereas interrater reliability error turned out to be very low. Second, increasing the number of test scorer was ineffective in seeking the optimal condition of originality grading on both standard and simplified versions, but results of the study show that much more testing items are necessary for the test to be more effective. The result suggests that using only 10 items of the TTCT Section 2, for the sake of convenience, can result in distortion of test results both in standard and simplified scoring versions. The present study also suggests the need for deep and multi-angular research for the optimization of TTCT grading system using a more advanced statistical approach.

다변량 일반화가능도 이론을 활용한 창의적 사고력 검사 (TTCT- 도형 A형) 독창성 평가 최적화 조건 탐색

이진아(LEE JIN A),한기순(Han ki Soon) 한국창의력교육학회 2017 창의력교육연구 Vol.17 No.2

본 연구에서는 TTCT 도형 A검사를 활용한 독창성 요소 측정 시 문항유형별(활동 1, 2, 3) 적절 문항수와 상대가중치 산출 조건을 탐색함으로써 독창성 평가 최적화를 위한 대안을 제시하는데 목적이 있다. 이를 위하여 수도권 지역 초등학생 61명을 대상으로 TTCT도형 A검사를 실시하여 정규형 버전과 간편형 버전으로 각각 채점을 하였다. 주요 결과를 요약하면 다음과 같다. 첫째, 각 채점 방법에서 문항유형별로 피험자 점수에 영향을 주는 요인들의 상대적 영향력 분석 결과 공통적으로 피험자와 문항의 상호작용에 의한 오차분산이 매우 큰 것으로 나타났으며, 피험자 오차 분산보다 문항 오차 분산은 상대적으로 높게 나타났다. 둘째, 각 채점 기준에서 합성 점수 산출 시 간편형 채점기준에서는 일반화가능도 계수에서 적합신뢰도에 못미친 반면 정규형 채점기준에서는 .8이상의 신뢰로운 결과값이 나타났다. 간편형 채점기준은 최적 문항 유형별 상대 가중치 조건이 산출되지 못하였으며, 정규형 채점기준은 현행 상대가중치인 .18 : .82에서 오차분산이 높은 활동 2에 가중치를 더한 .2 : .8의 조건에서도 적합 신뢰도로 나타났다. 마지막으로 각 각의 채점 방법에서 유형별 문항 수와 상대가중치 조합 조절을 통해 재분석해 본 결과 간편형 채점기준의 경우는 문항 수 및 상대가중치 조합을 변화시켜도 신뢰도 계수값을 만족하지 못했으며, 정규형 채점기준의 경우는 활동 3의 문항수를 최대 10개까지 줄여도 적합 신뢰도값을 보였다. 이때 문항유형별 최적 상대가중치는 활동 2와 활동 3이 각각 .2 : .8이었다. 즉, 연구 결과 정규형 채점기준에서만 신뢰성과 효율성을 만족하는 최적의 측정 조건 구성이 도출되었다. 더불어 검사실시의 시간적 어려움으로 활동 2만을 활용하여 독창성을 측정한다면 필히 신뢰도를 확보할 대안 마련 및 검증이 선행되어야 한다는 시사점을 얻을 수 있었다. This research is to find the right number of questions per category and an ideal set of conditions for computing relative significance value, thus providing an alternative as a better originality assessment tool. 61 elementary school children took the TTCT figural A and were each reviewed by both regular scoring version and steamlined version. Here is the summary of meaningful outcome. First, in each scoring method, relative influences of factors affecting the subjects’ score were analyzed and it was shown that each testing method had in common a high error variance from interaction between the subjects and items. Also, subject error variance was shown relatively higher than items error variance. Second, when computing the total score, steamed version was short of reliability on generalizability coefficient whereas regular version showed a reliable result above .8. Steamlined version was inadequate to produce ideal condition for relative significance and regular version has shown adequate reliability under current relative significance value, .18 : .82, as well as under .2 : .8, a weighted value on activity 2, which showed high error variance. Lastly, each scoring method was reanalyzed under different combinations of question per category and relative significance. Steamlined version didn’t satisfy the reliability coefficient value under any combinations and regular version showed acceptable reliability value when number of questions in activity 3 was reduced by up to 10. At that point, optimal relative significance on activity 2 and 3 were .2 : .8. In summary, optimal test condition that satisfies both reliability and efficiency was produced only in regular scoring version. Also, when performing the test with only activity 2, perhaps due to lack of time, results of the research underscored the need to find solution to secure reliability.

부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

순창군 장류로부터 분리된 황국균의 동정 및 특성

임은미 ( Eun Mi Lim ),이지영 ( Ji Young Lee ),모하메드 ( Mohammed A. Abdo Elgabbar ),한갑훈 ( Kap Hoon Han ),이보순 ( Bo Soon Lee ),조용식 ( Yong Sik Cho ),김현영 ( Hyoun Young Kim ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.4

In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify Aspergillus oryzae from fungal isolates. Ten fungal isolates were identified with β-tubulin gene. According to the sequences of β-tubulin gene, ten fungal isolates were identified as A. oryzae/flavus complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of A. oryzae and A. flavus strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of norB and cypA intergenic region, in which a deletion was recognized relative to A. flavus and A. parasiticus. The amplicon size of the ten fungal isolate strains was smaller than those of A. flavus and A. parasiticus, but the same as that of the reference A. oryzae strain. These results indicated that the ten isolates should be identified as A. oryzae. The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the A. oryzae isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.

한국인에서 5,10-Methylenetetrahydrofolate Reductase 유전자 다형성 분석 및 임신의 결과에 미치는 영향

김아리 ( Ari Kim ),강은지 ( Eun Ji Kang ),이경아 ( Kyung A Lee ),박세나 ( Se Na Park ),박종순 ( Jong Soon Park ),박보현 ( Bo Hyun Park ),박혜숙 ( Hyesook Park ),박미혜 ( Mi Hye Park ),전선희 ( Sun Hee Chun ),안정자 ( Jung Ja Ahn ) 대한주산의학회 2006 大韓周産醫學會雜誌 Vol.17 No.3

목적: 본 연구는 한국인 임신 여성에서 MTHFR 유전자형 변이와 혈청내 호모시스테인 및 엽산의 농도와 이들이 임신에 미치는 영향을 알아보고자 하였다. 방법: 임신부 600명에게서 혈액을 채취하여 얻어진 DNA로 MTHFR C677T와 A1298C 유전자의 다형성을 PCR-RELP로 분석하였고 혈청내 호모시스테인은 HPLC 방법으로, 엽산은 RIA 방법으로 측정하였다. 결과: 혈청내 호모시스테인은 MTHFR C677T 유전자형이 C/C형이나 C/T인 군에 비해 T/T인 군에서 유의하게 높았으며 MTHFR A1298C 유전자형이 A/C형이나 C/C인 군에 비해 A/A인 군에서 역시 유의하게 높았다( p<0.05). 혈청 호모시스테인은 모든 MTHFR 유전자형에서 혈청 엽산농도와 역상관관계를 보였으나, 변이형 유전자형(T/T와 A/A유전자형)에서 더 큰 역 상관관계를 보였다. 15?mol/L 이상의 고호모시스테인혈증의 임신부에서 임신 주수와 출생체중이 36.1주, 3053.8 g으로 15 ?mol/L미만인 군의 38.3 주, 3215.3 g 보다 유의하게 낮았다( p<0.05). 결론: 혈청 호모시스테인의 농도는 MTHFR C677T와 A1298T 유전자의 다형성에 의해 영향을 받으며, MTHFR C677T와 A1298T 유전자의 다형성과 혈청 호모시스테인은 모두 임신주수와 신생아의 체중에 영향을 미친다. Objective: The purpose of this study was to analyze MTHFR polymorphism among the Korean population and to evaluate the relationship between serum levels of homocysteine and MTHFR polymorphism and also to investigate the effect on pregnancy outcomes. Methods: DNA was extracted from whole blood of 600 pregnant women. All samples were genotyped for the C677T and A1298C polymorphisms in MTHFR gene by PCR-RELP assay. Serum levels of homocysteine and folate were measured by high performance liquid chromatography for homocysteine and radioassay for folate. Pregnancy outcomes were estimated by gestational weeks and birth weights of newborns. Results: Serum homocysteine was higher in women with the T/T genotype than those with the C/T or C/C genotype of the MTHFR C677T polymorphism (p<0.05). And also serum homocysteine was higher in women with the A/A genotype than those with the A/C or C/C genotype of the MTHFR A1298C polymorphism (p<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes, especially prominent in T/T genotype of MTHFR C677T polymorphism and A/A genotype of MTHFR A1298C polymorphism. Gestational age and the birth weight of infant from hyperhomocysteinemic mothers whose homocysteine levels higher than 15 μmol/L were 36.1 weeks, 3053.8 g, respectively, which were significant lower than those from normohomocysteinemic mothers (38.3 weeks, 3,215.3 g) (p<0.05). Conclusion: Serum homocysteine was influenced significantly by MTHFR C677T polymorphism and MTHFR A1298C polymorphism. MTHFR C677T and A1298C polymorphism and serum homocysteine levels affect pregnancy outcomes, although not mainly by serum folate level.

Several Regions in the Major Histocompatibility Complex Confer Risk for Anti-CCP-Antibody Positive Rheumatoid Arthritis, Independent of the DRB1 Locus

Lee, Hye-Soon,Lee, Annette T.,Criswell, Lindsey A.,Seldin, Michael F.,Amos, Christopher I.,Carulli, John P.,Navarrete, Cristina,Remmers, Elaine F.,Kastner, Daniel L.,Plenge, Robert M.,Li, Wentian,Greg Springer (Biomed Central Ltd.) 2008 Molecular Medicine Vol.14 No.5

<P>Recent evidence suggests that additional risk loci for RA are present in the major histocompatibility complex (MHC), independent of the class II HLA-DRB1 locus. We have now tested a total of 1,769 SNPs across 7.5Mb of the MHC located from 6p22.2 (26.03 Mb) to 6p21.32 (33.59 Mb) derived from the Illumina 550K Beadchip (Illumina, San Diego, CA, USA). For an initial analysis in the whole dataset (869 RA CCP + cases, 1,193 controls), the strongest association signal was observed in markers near the HLA-DRB1 locus, with additional evidence for association extending out into the Class I HLA region. To avoid confounding that may arise due to linkage disequilibrium with DRB1 alleles, we analyzed a subset of the data by matching cases and controls by DRB1 genotype (both alleles matched 1:1), yielding a set of 372 cases with 372 controls. This analysis revealed the presence of at least two regions of association with RA in the Class I region, independent of DRB1 genotype. SNP alleles found on the conserved A1-B8-DR3 (8.1) haplotype show the strongest evidence of positive association (P ~ 0.00005) clustered in the region around the HLA-C locus. In addition, we identified risk alleles that are not present on the 8.1 haplotype, with maximal association signals (P ~ 0.001-0.0027) located near the ZNF311 locus. This latter association is enriched in DRB1*0404 individuals. Finally, several additional association signals were found in the extreme centromeric portion of the MHC, in regions containing the DOB1, TAP2, DPB1, and COL11A2 genes. These data emphasize that further analysis of the MHC is likely to reveal genetic risk factors for rheumatoid arthritis that are independent of the DRB1 shared epitope alleles.</P>

건강인에서 연령, 성별 및 ABO 혈액형에 따른 혈장 von Willebrand Factor 항원 농도의 차이

이안나,이경아,송경순 한국지혈혈전학회 1996 한국지혈혈전학회지 Vol.3 No.1

MOLECULAR EPIDEMIOLOGY OF AVIAN POXVIRUS IN THE ORIENTAL TURTLE DOVE (<i>STREPTOPELIA ORIENTALIS</i>) AND THE BITING MIDGE (<i>CULICOIDES ARAKAWAE</i>) IN THE REPUBLIC OF KOREA

Lee, Hae Rim,Koo, Bon-Sang,Kim, Jong-Taek,Kim, Heung-Chul,Kim, Myung-Soon,Klein, Terry A.,Shin, Man-Seok,Lee, Sanghun,Jeon, Eun-Ok,Min, Kyung-Cheol,Lee, Seung Baek,Bae, Yeonji,Mo, In-Pil Wildlife Disease Association 2017 JOURNAL OF WILDLIFE DISEASES Vol.53 No.4

<P>A total of 600 wild birds were analyzed for the causes of mortality in the Republic of Korea (ROK) from 2011 to 2013. Avian poxvirus (APV) infections were identified as the primary cause of mortality in 39% (29/74) Oriental Turtle Doves (Streptopelia orientalis). At necropsy, all 29 S. orientalis birds, of which, 76% (22/29) were juveniles, had severe diphtheritic lesions in their oral and nasal cavities and on their eyelids, which were the lesions of APV that resulted in mortality. We detected APV infection by chorioallantoic membrane inoculation and molecular study of the partial region of the P4b gene. All isolates belonged to the same APV strain and were identical to strains isolated from several different pigeon species in South Africa. Phylogenetically, the APV strain identified in S. orientalis belonged to subclade A2, which includes isolates from several species of pigeons from different parts of the world, including the United Kingdom, Germany, India, Egypt, Hawaii, Georgia, Hungary, South Africa, Tanzania, and the ROK. This identity indicated that this diphtheritic APV strain may be a potential pathogen of other pigeon species in the ROK and neighboring countries throughout the range of S. orientalis. However, reticuloendotheliosis virus insertion into the APV genome was not detected by PCR in any of the 29 APV infections. An identical strain of APV observed in S. orientalis was also detected in Culicoides arakawae (biting midge), with annual peak populations corresponding to the presence of APV in S. orientalis. Culicoides arakawae may be a primary vector of APV in S. orientalis. Active surveillance of APVs in wild birds and C. arakawae is needed to better understand the epidemiology of APVs, host-vector relationships, and its ecological effects on S. orientalis in the ROK.</P>

Aquitalea denitrificans sp. nov., isolated from a Korean wetland.

Lee, Chang-Muk,Weon, Hang-Yeon,Kim, Yoo-Jeong,Son, Jung-A,Yoon, Sang-Hong,Koo, Bon-Sung,Kwon, Soon-Wo Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.5

<P>A novel bacterium, designated strain 5YN1-3(T), was isolated from wetland peat collected from Yongneup, Korea. The bacterium was facultatively anaerobic, Gram-negative, yellow-coloured, rod-shaped, mesophilic and motile with one polar flagellum. The strain grew optimally at 30 degrees C, at pH 6.0-9.0 and with 0-1 % NaCl (w/v). 16S rRNA gene sequence analysis showed the highest similarity to the sequence from Aquitalea magnusonii TRO-001DR8(T), with 98.7 % sequence similarity. However, strain 5YN1-3(T) showed DNA-DNA relatedness of 43 % (40 % in a reciprocal experiment) with A. magnusonii LMG 23054(T). The strain contained summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c) and C(16 : 0) as major cellular fatty acids. On the basis of DNA-DNA relatedness and physiological and biochemical characterization, strain 5YN1-3(T) should be assigned to a novel species of the genus Aquitalea, for which the name Aquitalea denitrificans sp. nov. is proposed. The type strain is 5YN1-3(T) (=KACC 12729(T) =DSM 21300(T)).</P>