Atomoxetine and Fluoxetine Activate AMPK-ACC-CPT1 Pathway in Human SH-SY5Y and U-87 MG Cells

Songhee Jeon(Songhee Jeon),Jeong-Eun Park(Jeong-Eun Park),Young Ho Do(Young Ho Do),Renata Santos(Renata Santos ),Seong Mi Lee(Seong Mi Lee),Bung-Nyun Kim(Bung-Nyun Kim),Jae Hoon Cheong(Jae Hoon Cheong 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.3

Objective Atomoxetine and fluoxetine are psychopharmacologic agents associated with loss of appetite and weight. Adenosine monophosphate-activated protein kinase (AMPK) is the cellular energy sensor that regulate metabolism and energy, being activated by fasting and inhibited by feeding in the hypothalamus. Methods Human brain cell lines (SH-SY5Y and U-87 MG cells) were used to study the outcome of atomoxetine and fluoxetine treatment in the activity of AMPK-acetyl-CoA carboxylase (ACC)- carnitine palmitoyl transferase 1 (CPT1) pathway and upstream regulation by calcium/calmodulin-dependent kinase kinase β (CaMKKβ) using immunoblotting and CPT1 enzymatic activity measures. Results Phosphorylation of AMPK and ACC increased significantly after atomoxetine and fluoxetine treatment in the first 30-60 minutes of treatment in the two cell lines. Activation of AMPK and inhibition of ACC was associated with an increase by 5-fold of mitochondrial CPT1 activity. Although the neuronal isoform CPT1C could be detected by immunoblotting, activity was not changed by the drug treatments. In addition, the increase in phospho-AMPK and phospho-ACC expression induced by atomoxetine was abolished by treatment with STO-609, a CaMKKβ inhibitor, indicating that AMPK-ACC-CPT1 pathway is activated through CaMKKβ phosphorylation. Conclusion These findings indicate that at the cellular level atomoxetine and fluoxetine treatments may activate AMPK-ACC-CPT1 pathways through CaMKKβ in human SH-SY5Y and U-87 MG cells.

Road Speed Prediction Scheme Based on Machine Learning Considering Road Environment Data

Songhee Park,DoJin Choi,KyoungSoo Bok,JaeSoo Yoo 한국콘텐츠학회 2019 한국콘텐츠학회 ICCC 논문집 Vol.2019 No.12

Smart Traffic Congestion Prediction Scheme for Safety Traffic Service

Songhee Park,DoJin Choi,KyoungSoo Bok,JaeSoo Yoo 한국콘텐츠학회 2018 한국콘텐츠학회 ICCC 논문집 Vol.2018 No.12

Analysis of agricultural characteristics of risotto rice varieties in Korean environment

Songhee Park,Jeonghwan Seo,Jae-Ryoung Park,Kyeong Min Kang,Mira Yoon,Mina Jin,O-Young Jeong,Hyun-Su Park 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

교통 돌발 상황을 고려한 도로 속도 예측 기법

박송희(Songhee Park),최도진(Dojin Choi),복경수(Kyoungsoo Bok),유재수(Jaesoo Yoo) 한국콘텐츠학회 2020 한국콘텐츠학회논문지 Vol.20 No.4

교통 혼잡으로 인한 사회적 비용이 증가하면서 도로 속도를 예측하기 위한 다양한 연구들이 진행되고 있다. 도로 속도 예측의 정확도를 향상시키기 위해서는 교통 돌발 상황을 고려할 필요가 있다. 본 논문에서는 교통돌발 상황을 고려한 도로 속도 예측 기법을 제안한다. 제안하는 기법은 연결된 도로들이 미치는 영향을 반영하기 위해서 예측 도로의 속도 데이터 뿐만 아니라 연결된 도로들의 속도 데이터도 이용한다. 또한, 돌발 상황으로 인한 혼잡을 예측하기 위해 속도의 변화량을 분석한다. 연결된 도로와 타겟 도로의 속도 데이터를 LSTM의 입력 데이터로 이용하여 1차적으로 도로 속도를 예측한다. 교통 돌발 상황으로 도로의 규칙적인 흐름이 깨지며 발생하는 예측 오차를 줄이기 위해 이벤트 가중치를 적용하여 최종적으로 도로 속도를 예측한다. 다양한 성능 평가를 통해 제안된 방법의 우수성을 입증한다. As social costs of traffic congestion increase, various studies are underway to predict road speed. In order to improve the accuracy of road speed prediction, unexpected traffic situations need to be considered. In this paper, we propose a road speed prediction scheme considering traffic incidents affecting road speed. We use not only the speed data of the target road but also the speed data of the connected roads to reflect the impact of the connected roads. We also analyze the amount of speed change to predict the traffic congestion caused by traffic incidents. We use the speed data of connected roads and target road with input data to predict road speed in the first place. To reduce the prediction error caused by breaking the regular road flow due to traffic incidents, we predict the final road speed by applying event weights. It is shown through various performance evaluations that the proposed method outperforms the existing methods.

COVID-19 진단기법: Primer에 따른 SARS-CoV-2 (COVID-19)의 핵산기반 진단법 분석

박송희(Songhee Park) 한국생물공학회 2021 KSBB Journal Vol.36 No.1

Since the first outbreak in Wuhan, China, Coronavirus Disease 2019 (COVID-19) has spread rapidly around the world and there are still many confirmed cases worldwide. In this serious situation, the rapid diagnosis and classification of patients who are infected with COVID-19 are very important to individuals and society. There are two main methods of testing in COVID-19: nucleic acid testing and immunological testing, but the test currently used in the confirmatory assay is nucleic acid testing, due to its accuracy. This nucleic acid test uses RT-PCR which is using reverse transcriptase, because SARS-CoV-2 is RNA virus. One of the important factors determining accuracy in RT-PCR is the primer sequences. In other words, it is very important for the accuracy of the test that it adheres exactly to the target gene. Therefore, this review focused on primer and described the test methods for SARS-CoV-2 detection. The main targets of the primers in SARS-CoV-2 were ORF1ab/RdRp gene, E gene, N gene, and S gene. I conducted comparisons of several primer probe sets of these target genes, and also checked the primer configuration of several commercial diagnostic kits to inspect the actual utilization. As a result, most of the genes I mentioned were frequently used as targets, and most of them were highly reliable.

REBT 집단상담을 적용한 원예활동이 결혼이주 여성의 자기표현과 우울에 미치는 영향

박송희(Songhee Park),정태열(Taeyeol Jung) 인간식물환경학회 2015 인간식물환경학회 학술대회 Vol.2015 No.추계

Leaching Characteristics of Herbicide Pendimethalin in Upland Soil Using Undisturbed Lysimeter

Jeonghwon Park,Sungjin Lim,Youngtak Oh,Hyeonggon Joo,Minho Lee,Hyoin Yoon,Geunhyoung Choi,Songhee Ryu,Byungjun Park 한국농약과학회 2017 한국농약과학회 학술발표대회 논문집 Vol.2017 No.11

성폭력 가해 아동에 대한 공적 개입 개선방안

박송희 ( Park Songhee ),진혜경 ( Jin Hyekyung ) 한국학교사회복지학회 2021 학교사회복지 Vol.- No.53

본 연구는 성폭력 피해 아동 중심의 아동 복지정책에서 더 나아가 성폭력 가해 아동에 대한 공적 개입내용을 살펴보고 개선방안을 모색하기 위한 탐색적 연구이다. 이를 위하여 성범죄 가해 아동에 대한 공적 개입의 이론적 근거를 살펴보고, 경찰, 학교, 법원, 보호관찰소의 개입실태를 살펴보았다. 주로 문헌 연구 방법을 사용하였으며, 각 기관에 대한 보고서와 정보공개를 요청하여 추가된 자료를 수집하고 분석하였다. 연구 결과 현재의 공적 개입 체계하에서는 성폭력 가해 아동 현황과 사법처리 결과 등 통합적인 통계관리가 이루어지지 않고 있으며, 학교와 경찰 그리고 법원을 거치는 동안 재발 방지를 위한 치료 프로그램 또한 미미한 수준으로 드러났다. 이를 토대로 성폭력 가해 아동에 대한 생태체계적 관점과 국친 사상을 바탕으로 한 복지적 개입이 절실히 필요하며, 특히 형사처벌을 받지 않는 14세 미만 성폭력 가해 아동에 대해서는 학교나 경찰의 사건 초기 단계부터 법원의 보호처분 이후까지 세심하고 촘촘한 공적 개입이 이루어져야 할 것으로 분석되었다. 따라서 각각의 공적 개입 체계 단계마다 성폭력 가해 아동에 대한 기초 통계자료 관리와 통합적 재발방지 프로그램의 가이드 라인 마련이 필요함을 제시하였다. 또한, 생태체계적 관점을 바탕으로 각 공적 기관 간의 촘촘한 네트워크 구축과 함께 가해 아동에 대한 체계적이고 지속 가능한 사례관리를 위해 학교 사회복지의 제도화가 중요함을 제안하였다. This study is an exploratory study looking into the current public interventions for children who commit sexual assault in order to find ways to improve them as most children welfare policies today are focused on the victims rather than the perpetrators. To this end, the theoretical basis of public interventions against children who have committed sexual crimes was examined, as well as the current state of interventions in schools, police stations, courts, probation offices. The method used was a literature research, further additional data was collected and analysed via requested reports and information disclosure from each institution. As a result of the study, it was found that welfare interventions based on the ecological system perspective in addition to the Parens patriae on sexual violence children were necessary. In particular, it turned out that there is a desperate need for meticulous and close public intervention against children who have committed sexual assault under the age of 14 and are not subject to criminal punishment. Therefore, it was suggested that basic statistical data management and public intervention guidelines should be prepared for children who are perpetrators of sexual assault under the age of 14 in order to better instruct the police, school, court, and probation offices. In addition, the institutionalization of school social work for systematic and integrated case management and establishment of networks among public institutions was proposed based on the ecological system perspective for children who have committed sexual assault.

노인 병원 동행서비스의 인식과 요구도

박송희 ( Songhee Park ),김설희 ( Seolhee Kim ) 인문사회 21 2023 인문사회 21 Vol.14 No.1

연구 목적: 본 연구는 노인 병원 동행서비스 인식과 요구도를 조사하여 병원 동행서비스 확대에 필요한 기초 자료를 제공하고자 하였다. 연구 방법: 2022년 7~8월까지 대전광역시, 예산군에 거주하는 성인 142명을 대상으로 병원동행서비스 인식, 요구도에 관한 설문조사 후 SPSS 24.0 프로그램을 이용하여 기술통계, t-test, ANOVA 분석을 하였다. 연구 내용: 병원 동행서비스 인식은 25.4%, 요구는 86%. 지원대상은 의료취약계층이 높게 나타났다. 운영 요구도는 평일 7~20시, 동행 매니저는 보건분야 전문가, 동행 매니저 필요 교육은 친절교육이었다. 병원 동행서비스 운영 기대효과는 의료 적시 이용(4.32±0.55), 인력 양성(4.25±0.60), 사회안전망 구축(4.20±0.64)이 높게 나타났다(p<0.05). 결론 및 제언: 노인 및 1인 가구 증가에 따라 혼자서 병원을 이용하지 못하는 대상의 병원 동행서비스 요구가 높았고 노인 및 의료취약계층의 의료 이용에 긍정적 영향을 미치는 방안임을 확인할 수 있어서 일부 지역에서 시행하는 병원 동행서비스의 확대 방안이 요구되었다. The purpose of this study is to provide basic data necessary for the expansion of hospital companion service by investigating the recognition and demand for the hospital companion service for the elderly. From July to August 2022, 142 adults residing in Yesan-gun, Daejeon Metropolitan City were surveyed on the perception and demand for hospital companion service. The collected data were subjected to descriptive statistics, t-test, and ANOVA analysis using the SPSS 24.0 program. Perception of hospital companion service was 25.4% and 86% demanded it. The target of support was high in the medically vulnerable group (p<0.05). As for the operating requirements, the hours were 07-20, the manager was a health expert, and the manager training was high in kind service. The expected effect was high in medical care timely use (4.32±0.55), manpower training (4.25±0.60), and social safety net establishment (4.20±0.64) (p<0.05). The perception that the elderly and single-person households who cannot go to the hospital alone need safe accompaniment service was high. It was confirmed that it is a method that has a positive effect on the use of hospital by the elderly and the medically disadvantaged class. Therefore the nationwide expansion of companion service implemented in some regions was required.