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Mina Kim(Mina Kim),Soo Jin Yang(Soo Jin Yang),Hyang Hee Kim(Hyang Hee Kim),Anna Jo(Anna Jo),Min Jhon(Min Jhon),Ju-Yeon Lee(Ju-Yeon Lee),Seung-Hyung Ryu(Seung-Hyung Ryu),Jae-Min Kim(Jae-Min Kim),Young- 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.1
Objective: To investigate the effects of dietary habits on general and abdominal obesity in community-dwelling patients with schizophrenia spectrum disorder according to sex. Methods: A total of 270 patients with schizophrenia spectrum disorder registered at mental health welfare centers and rehabilitation facilities were recruited. General obesity was defined as a body mass index ≥ 30 kg/㎡, and abdominal obesity was defined as a waist circumstance ≥ 90 cm in men and ≥ 85 cm in women. Dietary habits were evaluated using dietary guidelines published by the Korean Ministry of Health and Welfare. Demographic and clinical characteristics along with dietary habits and information related to obesity were collected. Factors related to obesity were evaluated separately by sex. Results: Dietary habits differed according to sex, in that scores for healthy eating habits were lower in men than in women. In men, the prevalences of general and abdominal obesity were 17.0% and 37.3%, respectively. In women, the prevalences of general and abdominal obesity were 23.1% and 38.5%, respectively. Regression analysis showed that the scores of regular eating habits were negatively associated with general and abdominal obesity in men, and the scores of healthy eating habits were negatively associated with general and abdominal obesity in women. Conclusion: Among patients with schizophrenia, regular eating habits might reduce the risk of obesity in men, and healthy eating habits might reduce the risk of obesity in women. Nutrition education should be provided to community-dwelling patients with schizophrenia to prevent obesity in this population.
Mina Jin,Mi-suk Seo,Sun-Ju Kim,Jin-Hyeok Chun,Beom-Seok Park,Seong-Han Sohn,Jung Sun Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Glucosinolates of Brassica rapa collection from Korea genebank were measured to determine total glucosinolate content and their variation of diverse glucosinolates; Around 100 accessions representing the different morphotypes and geographical origin of Brassica rapa were analysed. The principal component analysis was performed to evaluate the differences among morphotypes using the profiles of 14 glucosinolates identified from the leaves. DMRT test and box plots showed the significant difference between total glucosinolates of subspecies. Most of turnip accessions had higher gluconilates compared to the other type accessions, Chinese cabbage and pak choi. These accessions will be used for GWAS study for glucosinolate. Now they are being finger-printed by genotyping by sequencing (GBS). Among these accession, we selected a turnip accession with high amount of glucosinolate, K0466 and two Chinese cabbage accession with low amount of glucosinolate, K0015 and K0621. To analyse quantitative traits loci (QTL) for glucosinolate synthesis, these three accessions were fixed through microspore culture. Finally, six homozygous lines were selected and were crossed each other to make F1 hybrids. We just harvested F2 seeds and transferred doubled haploid plants to pots. QTL analysis for glucosinolate will be performed these F2 and DH population.
Yang, Tae-Jin,Kim, Jung-Sun,Lim, Ki-Byung,Kwon, Soo-Jin,Kim, Jin-A,Jin, Mina,Park, Jee Young,Lim, Myung-Ho,Kim, Ho-Il,Kim, Seog Hyung,Lim, Yong Pyo,Park, Beom-Seok Hindawi Publishing Corporation 2005 Comparative and functional genomics Vol.6 No.3
<P>A complete genome sequence provides unlimited information in the sequenced organism as well as in related taxa. According to the guidance of the Multinational Brassica Genome Project (MBGP), the Korea Brassica Genome Project (KBGP) is sequencing chromosome 1 (cytogenetically oriented chromosome #1) of <I>Brassica rapa</I>. We have selected 48 seed BACs on chromosome 1 using EST genetic markers and FISH analyses. Among them, 30 BAC clones have been sequenced and 18 are on the way. Comparative genome analyses of the EST sequences and sequenced BAC clones from <I>Brassica</I> chromosome 1 revealed their homeologous partner regions on the <I>Arabidopsis</I> genome and a syntenic comparative map between <I>Brassica</I> chromosome 1 and <I>Arabidopsis</I> chromosomes. <I>In silico</I> chromosome walking and clone validation have been successfully applied to extending sequence contigs based on the comparative map and BAC end sequences. In addition, we have defined the (peri)centromeric heterochromatin blocks with centromeric tandem repeats, rDNA and centromeric retrotransposons. In-depth sequence analyses of five homeologous BAC clones and an <I>Arabidopsis</I> chromosomal region reveal overall co-linearity, with 82% sequence similarity. The data indicate that the <I>Brassica</I> genome has undergone triplication and subsequent gene losses after the divergence of <I>Arabidopsis</I> and <I>Brassica</I>. Based on in-depth comparative genome analyses, we propose a comparative genomics approach for conquering the <I>Brassica</I> genome. In 2005 we intend to construct an integrated physical map, including sequence information from 500 BAC clones and integration of fingerprinting data and end sequence data of more than 100 000 BAC clones. The sequences have been submitted to GenBank with accession numbers: 10 204 BAC ends of the KBrH library (CW978640–CW988843); KBrH138P04, AC155338; KBrH117N09, AC155337; KBrH097M21, AC155348; KBrH093K03, AC155347; KBrH081N08, AC155346; KBrH080L24, AC155345; KBrH077A05, AC155343; KBrH020D15, AC155340; KBrH015H17, AC155339; KBrH001H24, AC155335; KBrH080A08, AC155344; KBrH004D11, AC155341; KBrH117M18, AC146875; KBrH052O08, AC155342.</P>
Minimum Tiling Path BAC Clones of Mitochondria and Chloroplast Genome of Brassica rapa
Jee Young Park,Tae Jin Yang,Ki Byung Lim,Soo Jin Kwon,Jung Sun Kim,Jin A Kim,Mina Jin,Myung Ho Lim,Beom Soon Choi,Hyo Jin Lee,Ho Il Kim,Seok Hyoung Kim,Yong Pyo LimBeom Seok 한국육종학회 2005 한국육종학회 학술발표회 발표요지 Vol.37 No.1
Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.
Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3
<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>