Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

하악 영구 견치의 의원성 매복에 대한 증례 보고

김송이,최성철,최영철,박재홍 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

상악 영구 견치의 매복에 비해 하악 영구 견치의 매복은 매우 드물게 일어난다. 치아가 매복된 경우 장애물 존재 여부와 매복 위치와 방향,맹출 가능한 공간의 유무,치근의 형성 단계 등의 요소에 따라 매복 치아의 발거 혹은 장애물의 제거 후 주기적 관찰,맹출이 지연된 치아의 외과적 노출,교정적 견인 등의 방법으로 치료하게 된다. 본 증례는 하악골의 골절 부위에 시행된 골간 강선 결찰과 미니 플레이트에 의해 하악 좌측 견치가 매복되어 있었던 경우로 CT 검사 결과 하악 정중부위 플레이트의 스크류가 맹출을 방해하고 있는 것으로 판단되어 전선 마취 하에 플레이트를 제거하였다. 이후 자연적인 맹출을 기대하였으나,맹출력이 부족하여 교정적 견인으로 양호한 결과를 얻었기에 보고하는 바이다. When a delayed eruption of tooth is diagnosed, the causative factor should be detected before an establishment of treatment plan, if possible. Although a panoramic radiograph is enough to evaluate the position of tooth and the stage of tooth development, a 3-D Dental CT would be a powerful tool to reveal a spatial relationships between objects. The reported case showed a delayed eruption of lower left permanent canine and a mini-plate with screws adjacent to the impacted canine. Although the screws adjacent to the root of impacted tooth showed a close proximity, it was not presumed that these screws would interfere the eruption of the tooth. The impacted canine did not show any spontaneous eruption during observation. After the mini-plate and screws were removed, an orthodontic traction using elastic power chain was performed. The position of mini-plates and screws should be carefully designed to avoid damaging the tooth follicles or tooth roots in the jaw. Also the screws should be removed before an orthodontic traction to prevent damaging the root surface of impacted tooth.

수종의 불소방출 수복재의 탈회억제 효과 및 불소침투에 관한 연구

김송이,최성철,최영철,김광철,박재홍 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

자이오머(Giomer)는 레진강화형글라스아이오노머에 견줄 만큼 불소방출량이 많다고 하나, 우식예방 효과에 관한 연구들이 많지 않아 임상적용에 대한 근거가 부족한 실정이다. 본 연구는 자이오머(BF 군)를 포함하여 레진강화형글라스아이오노머(FF 군), 콤포머(DA 군)와 대조군인 콤포짓(FZ 군)의 우식억제 효과와 주변 치질로의 불소침투 양상을 비교하기 위하여, 공초점레이저주사현미경과 전자탐침미세분석기를 이용하여 각 수복재의 우식예방 효과를 비교하였으며, 다음과 같은 결과를 얻었다. 1. 수복물 주위 이차 인공우식에서 법랑질 외측병변의 깊이는 FZ 군에서 가장 깊었으며, BF 군이 가장 작게 나타났다(p<0.05). 2. 수복물과 법랑질 계면에 나타난 탈회억제층의 두께를 관찰한 결과 FZ 군이 가장 작게 나타났으며, FF, DA, BF 간에는 유의성 있는 차이를 보이지 않았다(p<0.05). 3. 법랑질에 침투된 불소농도는 BF, FF, DA의 순으로 높게 나타났다. 4. 상아질에 침투된 불소농도는 DA, BF, FF의 순으로 높게 나타났으며, 이는 상아질-수복물의 접착 계면에 영향 받는 것으로 보인다. The purpose of this study was to evaluate the effect of light cured fluoride-releasing materials on the inhibition of demineralization. In addition, the pattern of fluoride uptake of adjacent tooth structure was analyzed with EPMA. Eighty intact premolars were restored with Filtek Z250(control group, composite), Fuji Filling LC(RMGI), Dyract AP (compomer) and Beautifil II(giomer). Restored teeth were stored in distilled water for 30 days. Thensixty teeth(n=15) were exposed to demineralizing solution(pH 4.3). Demineralized teeth were bisected and polished. The specimens were observed with confocal laser scanning microscope. The depth of outer lesion and the thickness of inhibition zone were measured. Remained twenty teeth(n=5) were bisected for fluoride uptake analysis. The fluoride analysis were taken at enamel-restoration interface and dentin-restoration interface by electron probe micro-analyzer. The results are as follows: 1. The depth of outer lesion of Fuji Filling LC, Dyract AP, Beautifil II was shallower than that of Filtek Z250 at the margin of restoration(p<0.05). 2. The thickness of caries inhibition zone of Fuji Filling LC, Dyract AP, Beautifil II was greater than that of Filtek Z250 at the margin of restoration(p<0.05). 3. Fuji Filling LC, Dyract AP, Beautifil II groups showed the greater fluoride uptake into enamel and dentine around restoration than Filtek Z250 group. 4. In dentin the difference of fluoride concentration were greater than in enamel, and Dyract AP showed the greatest fluoride concentration in dentin.

洞報 廢鑛山 周邊地域 土壤 內 重金屬 移動 分布

문영옥,정송송이,최상민,김민철,박창웅,오광중 부산대학교 환경문제연구소 2003 環境硏究報 Vol.21 No.-

In this paper, we study heavy metal transport and distribution of soils around Dong-bo abandoned mine area. Inside the mine, concentration of Cd, Ca, Zn are exceeded soil management standards. Outside of mine, concentration of Pb, as is exceeded soil management standards. Especially, concentration of Pb is higher than soil protection standard of 300㎎/㎏ in S-6 point which is tailing accumulation area. Although heavy metal concentration of mine water was higher than Unsuchon, water contaminated level was lower than soil. 30cm-depth soil is serious in S-6 point, heavy metal transport had influence on Unsuchon. Further more, we used pollution index(PI) to evaluate soil contamination totally, it exceeded standard "1" in S-6 points of surface soil and 30cm-depth soil. So it decided that contamination of heavy metal is more seriously than other sampling points. In contaminated soil, pH and organic contents are lower than non-contaminated soil. Therefore heavy metal concentration has influence on adsorption and retardation of soil by pH and organic contents variation. Moisture contents also encouraged heavy metal transport velocity, but less than pH and organic contents.

난소종양으로 오진된 골반후복막 신경집종 1예

박연진, 홍승화, 지일운, 정은환, 최송이, 김학순 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.2

본문참고

L1 cell adhesion molecule as a novel independent poor prognostic factor in gallbladder carcinoma

Choi, Song-Yi,Jo, Young Suk,Huang, Song-Mei,Liang, Zhe Long,Min, Jeong-Ki,Hong, Hyo Jeong,Kim, Jin-Man Elsevier 2011 Human pathology Vol.42 No.10

<P><B>Summary</B></P><P>Gallbladder carcinoma is a lethal malignancy and is hard to cure by current treatment. Thus, identification of molecular prognostic markers to predict gallbladder carcinoma as therapeutic targets is urgently needed. Recent studies have demonstrated that L1 cell adhesion molecule is associated with the prognosis of variable malignancy. Here, we investigated L1 cell adhesion molecule expression in gallbladder carcinoma and its prognostic significance. In this study, we examined L1 cell adhesion molecule expression in tumor specimens from 69 patients with gallbladder carcinoma by immunohistochemistry and analyzed the correlation between L1 cell adhesion molecule expression and clinicopathologic factors or survival. L1 cell adhesion molecule was not expressed in the normal epithelium of the gallbladder but in 63.8% of gallbladder carcinomas, remarkably at the invasive front of the tumors. In addition, L1 cell adhesion molecule expression was significantly associated with high histologic grade, advanced pathologic T stage and clinical stage, and positive venous/lymphatic invasion. Multivariate analyses showed that L1 cell adhesion molecule expression (hazard ratio, 3.503; <I>P</I> = .028) and clinical stage (hazard ratio, 3.091; <I>P</I> = .042) were independent risk factor for disease-free survival. L1 cell adhesion molecule expression in gallbladder carcinoma was significantly correlated with tumor progression and unfavorable clinicopathologic features. L1 cell adhesion molecule expression was an independent poor prognostic factor for disease-free survival in patients with gallbladder carcinoma. Taken together, our findings suggest that L1 cell adhesion molecule expression could be used as a novel prognostic factor for patient survival and might be a potential therapeutic target in gallbladder carcinomas.</P>

Electrocatalytic Reduction of Carbon Dioxide on Sn-Pb Alloy Electrodes

Choi, Song Yi,Jeong, Soon Kwan,Park, Ki Tae 한국기후변화학회 2016 한국기후변화학회지 Vol.7 No.3

Production of Recombinant Human Keratinocyte Growth Factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 Cells

Song-Yi Han(한송이),Cho-Yi Jin(진초이),Kisang Kwon(권기상),Eun Young Yun(윤은영),Tae Won Goo(구태원),Seung-Whan Kim(김승환),Jong-Soon Choi(최종순),Kweon Yu(유권),O-Yu Kwon(권오유) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

인간형 재조합단백질 각질세포 성장인자를 안정적으로 생산하는 누에 배양 세포(Bm5-hKGF cell)을 만들었다, 이 세포에서 분비되어 배지에 포함된 양은 15-20 ng/㎖ 정도였다. Bm5-hKGF cell에 누에의 PDI를 함께 발현시키면 세포외 분비량이 2배 증가하였다. Wound healing migration assay 결과 누에세포에서 생산된 인간형 재조합단백질 각질세포 성장인자는 세포생장을 촉진하는 활성을 가지고 있었다. 본 실험의 결과는 누에배양세포를 사용하여 저비용으로 양질의 인간형 재조합단백질을 대량생산 할 수 있는 것을 기대한다. Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human keratinocyte growth factor (hKGF), named by the Bm5-hKGF cell, in which the protein hKGF is synthesized in the cell and secreted in the cell culture supernatant (CCS) at approximately 15-20 ng/㎖. When the Bm5-hKGF cell was co-expressed with B. mori protein disulfide isomerase (bPDI) cDNA, its secretion increased by about two times the original amount. Through wound healing migration assay, it was demonstrated that the secreted hKGF included in the CCS has a very powerful biological activity of keratinocyte proliferation. We expect to produce useful human recombinant proteins from silkworm cultured cells in large quantities at low prices.

Carbon Dioxide Absorption into Aqueous Blends of Methyldiethanolamine (MDEA) and Alkyl Amines Containing Multiple Amino Groups

Choi, Song Yi,Nam, Sung Chan,Yoon, Yeo Il,Park, Ki Tae,Park, So-Jin American Chemical Society 2014 INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH - Vol.53 No.37

<P>Alkyl amines, which have multiple amino groups, are used as activators to improve the CO<SUB>2</SUB> absorption performances of aqueous methyldiethanolamine (MDEA) solutions. The aqueous MDEA blends consisted of 20% (w/w) of MDEA and 10% (w/w) of activators, which are 3-methylamino propylamine (MAPA), diethylenetriamine (DETA), triethylene tetramine (TETA), and tetraethylenepentamine (TEPA). Aqueous solutions of monoethanolamine (MEA; 30% (w/w)) and MDEA (30% (w/w)) are used as reference absorbents for comparison. The CO<SUB>2</SUB> absorption performances of aqueous MDEA blends were investigated by measurements of absorption capacities, absorption rates, and heats of absorption. The MDEA blends have higher CO<SUB>2</SUB> absorption capacities than MEA and MDEA. MDEA/TEPA shows the highest CO<SUB>2</SUB> loading amount of 0.753 mol-CO<SUB>2</SUB>·mol-absorbent<SUP>–1</SUP> at 313 K. In addition, the MDEA blends show high cyclic capacities (0.241–0.330 mol-CO<SUB>2</SUB>·mol-absorbent<SUP>–1</SUP>), the values of which are about 3 times higher than that of MEA. All MDEA blends show higher absorption fluxes than MDEA. The MDEA/MAPA showed the highest overall mass transfer coefficient of 3.351 × 10<SUP>3</SUP> mol·m<SUP>–2</SUP>·s<SUP>–1</SUP>·kPa<SUP>–1</SUP>, 8 times higher than that of MDEA (0.451 × 10<SUP>3</SUP> mol·m<SUP>–2</SUP>·s<SUP>–1</SUP>·kPa<SUP>–1</SUP>) and even higher than that of MEA (3.014 × 10<SUP>3</SUP> mol·m<SUP>–2</SUP>·s<SUP>–1</SUP>·kPa<SUP>–1</SUP>). The heats of absorption of the MDEA blends (57.21–59.53 kJ·mol-CO<SUB>2</SUB><SUP>–1</SUP>) are about 30% higher than that of MDEA and about 30% lower than that of MEA.</P>

Efficient method and evaluation of radish cultivars for resistance to Fusarium oxysporum f. sp. Raphani

Song Yi Baik,Jin Cheol Kim,Kyoung Soo Jang,Young Ho Choi,Gyung Ja Choi 한국육종학회 2010 한국육종학회 학술발표회 발표요지 Vol.42 No.1