Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

Song,Moon-Tae,Lee,Jeom-Ho,Lee,Sang-Bok,Cho,Youn-Sang,Ku,Ja-hwan,Seo,Kyoung-In,Choi,Seong-ho,Hwang,Heung-Goo 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

각종 악성종양질환에서의 세포면역 기능에 대한 연구 : 말초 혈액 T 임파구 아형 분포 및 지연형 과민반응에 대한 검색 Delayed Cutaneous Hypersensitivity and Peripheral Blood T-lymphocyte Subsets

송재화,이복희 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

The author studied delayed cutaneous hypersensitivity and peripheral blood T-lymphocyte subpopulations in 54 patients with malignant tumors. 1. The number and total score of positive reactions in delayed cutaneous hypersensitivity were significantly decreased in cancer patients(p<0.005, p<0.05). 2. Percent OKT4^+ T-lymphocyte in cancer patients were significantly decreased compared to normal control subjects (p <0. 05), and it had positive correlation with total score of delayed cutaneous hypersensitivity reactions(r=0.768). 3. Percent OKT8^+ cells were not different from that of normal control subjects. 4. The ratio of OKT4^+/OKT8^+ in cancer patients were not different from that of normal control subjects except in patients with colorectal carcinoma and malignant lymphomas. 5. Markers of activated T-lymphocyte, OKT10^+ and OKIal^+ cells were increased in all the patient groups. 6. Leu7^+ cells, reflecting natural killer cells, were not different from normal control subjects These results suggest that the cancer patients have depressed cellular immune functions, which mainly results from the decrease of helper T lymphocyte number. Short of functional assays of cellular immunity, the author concluded that the testing of delayed cutaneous hypersensitivity and enumeration of peripheral blood lymphocyte subsets, especially OKT4^+ cells and activated T cells provide useful informations on cellular immune reactivity in cancer patients. Further studies on prognostic value of these parameters should be pursued.

열처리에 따른 Zircaloy-4 Tube의 부식특성에 관한 연구

이동복,최송천 成均館大學校 科學技術硏究所 1994 論文集 Vol.45 No.1

열처리에 따른 Zircaloy-4 tube의 부식 특성을 조사하기 위하여 pilger된 시편, 수냉된 시편, 열처리된 시편에 대해, 500℃, 102 기압의 증기하에서의 부식 시험을 실시하였다. 주어진 시험조건하에서 수냉된 시편과 pilger된 시편은 좋은 nodular 부식 저항을 보였으나, 열처리된 시편은 나쁜 nodular 부식 저항을 가졌다. To investigate the effect of heat treatment on the corrosion behavior of water-quenched, heat -treated, and as-pilgered Zircaloy-4 tubes, corrosion testing was conducted at 500℃ in 102 atm of steam atmosphere. Under these given conditions, water-quenched and as-pilgered and the grains become bigger and equiaxed. Heat treated specimens exhibited improved resistance to nodular corroson, while heat- treated specimens had poor nodular corrosion resistance.

고능률 소형 전자석에 의한 자왜 및 자기이방성 측정

이용호,신용돌,김병걸,민복기,송재성 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

자왜 또는 자기이방성의 측정에 사용되는 전사석의 여자전류를 측정에 필요한 수초간만 흘려서 에너지 소모 및 측정시의 최대난점인 시료의 온도변화의 문제를 극소화하였다. 따라서 전자석의 냉각장치는 생략되고 크기와 전원의 용량도 극소화되었다. 공기간격 22㎜, 자극의 단면적 40 × 25㎟에서 0.5 T의 자장발생에는 180 W의 전원으로 족하였다. 시료의 자왜와, 자기이방성에 의한 토크를 전기용량센서에 의하여 측정하여 10^-8의 자왜분해능과 1 nJ의 토크 분해능을 얻었다. 0.02× 0.8× 10㎟의 연자성 리본의 형상이방성을 이용하여 이방성 측정시의 토크값을 교정하였다. A high efficiency small electromagnet(22㎜ air gap and 40 × 25㎟ core's cross section) suitable for measuring magnetostriction and magnetic anisotropy was built. The magnet could be miniaturized by reducing the measuring space and time. The excitation current of the electromagnet was supplied for only a few second of small. An 0.5 T magnetic field was generated with 180 W power consumption. The values of magnetostriction and magnetic anisotropy were measured with a very sensitive capacitance cell with resolution of 10^-8 and 1 no. The torque was calibrated using a soft magnetic ribbon's shape anisotropy.

진행위암 및 식도암에 대한 EAP병합 화학요법

송민호,이헌영,김삼용,김영건,이복희 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

Eleven patients with advanced gastric cancer, five patients with esophageal cancer were treated with Etoposide, Aclacinimycin (or Adraimycin), and Cisplatin(EAP) combination chemotherapy. The results were as followings: 1. The response rate was 64% in advanced stomach cancer and 60% in esophageal cancer. The overall response rate was 63% in total patients. 2. The toxicites of the EAP combination regimen were evaluated by ECOG(Eastern Cooperative Oncology Group) toxicity criteria. The toxicities were as following in order of frequency: anemia 44%, nausea and vomiting 37%, leukopenia 13%, genitorinary toxicites 7%, thrombocypenia 6%. 3. The median duration of survival was 7.5+ months stomach cancer and 8+ months in esophageal cancer in responded patients. 4. The median duration of partial remission were 4+ months and 5+ months in stomach cancer and esophageal cancer in respectively. In conclusion. the EAP combination chemotherapeutic regimen have substantial effects on advanced stomach and esophageal cancer.

장상피화생과 Helicobactor pylori와의 상관 관계에 관한 연구

이복희,김대수,이현숙,진영주,윤세진,송형근,성노현,정현용,이동호 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

In 1965 Lauren divided advanced gastric carcinoma into two main types-namely, "intestinal" and "diffuse" type carcinoma, which differ not only morphologically but also in their clinical and epidemiological characteristics. Moreover, a different histogenetic process has been postulated by many authors that intestinal type gastric carcinoma might arise from areas with intestinal metaplasia. Since Warren and Marshall reported the presence of Helicobacter pylori in the stomach in 1983, strong evidence has been obtained that there is a close relationship between Helicobacter pylori and chronic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal type gastric carcinoma. To study the relationship between intestinal metaplasia and Helicobacter pylori infection, we performed gastroscopic biopsies and CLO tests taken from patients with epigastric pain. Only 87 patients with macroscopically suspected antral gastritis were included. The prevalence of intestinal metaplasia in gastric antral biopsies increased from 33.3% in the age group<30 years to 63.6% in the age group 60 years. When all patients were divided into two age groups-namely, (i)<50 years(n=41) and (ii)≥ 50 years(n-=46), intestinal metaplasia was found significantly more often in patients ≥ 50 years. ((i) 39.2%, (ii) 54.3%, p<0.0001). The prevalence of Helicobacter pylori in gastric antral biopsies decreased from 66.7% in the age group<30 years to 62.5%, in the age group in the age group 50 to 59 years. When all patients were divided into the same two age groups-namely(i)<50 years and (ii)≥50 years, the prevalence of Helicobacter pylori did not differ between two groups in a statistically significant way. The mean age of intestinal metaplasia positive patients who were also Helicobacter pylori positive was 48±11.0(mean±S.D.)years, whereas the mean age of intestinal metaplasia positive patients who were Helicobacter pylori negative was 52±9.7(mean±S.D.)years. In conclusion. although our data do not prove a causal relationship between Helicobacter pylori infection and the histogenesis of intestinal metaplasia, we suggest that Helicobacter pylori plays an important role in the development of intestinal metaplasia in the gastric mucosa. Whether Helicobacter pylori has to be present at all times during this process or is only necessary as a 'trigger', needs further research.

경기도 강화지방의 민속 : -강화군 화도면 장화리를 중심으로-

조성선,이현복 仁川敎育大學畿甸文化硏究所 1980 기전문화연구 Vol.11 No.-

Ni_3Al 金屬間 化合物의 高溫酸化時 酸化膜의 剝離에 關한 硏究

崔松天,李東馥 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

The high temperature oxidation behavior of the intermetallic compound of pure Ni_3Al was studied above 1000℃. In isothermal oxidation tests between 1000-1200℃, Ni_3Al showed parabolic oxidation behavior and displayed excellent oxidation resistance. In cyclic oxidation test, Ni_3Al exhibited excellent oxidation resistance but the drastic spalling of oxide scales was observed at 1300℃. Al_2O_3 was present as θ-Al_2O_3 in a whisker form at 1000℃, but, as the temperature increases, thermodynamically metastable θ-Al_2O_3 was transformed to stable α-Al_2O_3. At 1200℃, α-Al_2O_3 having an equiaxed structure in the outermost scale and columnar structure inside was present. Scale spalling it Ni_3Al was attributed to the kirkendall voids formed at the metal/oxide interface, which act as the source of stress concentration.

충주시 농촌지역 주민들에 대한 보건교육 평가

권복규,양준호,송진범,이건세,이원진,장성훈 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.5 No.-

The aim of this study was to evaluate the effect of health education program in Choongju rural area which had conducted by Public Health Doctor during winter in 1994 and 1995. We used two step sampling method for a household survey. First, six were selected from 12 Myuns, which were stratified by the level of health education activity. Second, we applied convenient sample method. Of the 307 respondents, most of them knew Health-subcenter's existence in their region. However, only 149 persons(45%) visited it during last year. They visited Health-subcenter because it was easy to travel and convenient. Some of them thought that Health-subcenter had very poorly equipped and supplied. About 32∼34% of respondents participated the health education program for hypertension and diabetes mellitus which was done last winter, and 59∼70% of participants of the program answered that it was very, moderately helpful. 82∼83% of participants recognized complications of hypertension and diabetes mellitus, and 18∼20% of them did not know that medications of hypertension and diabetes mellitus should be continued one's whole life. The respondents want to receive health educations for cancer, arthritis, viral hepatitis and AIDS in orders. Many of rural residents utilize Health-subcenters because health clinics in Choongju rural area are very few.

Aspergillus candidus F1484 균주가 생산하는 항진균 화합물의 분리 및 특성

김성옥,이소영,김성규,손광희,김영국,문석식,복성해 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Candida albicans의 효모형태에 대해 활성을 나타내는 항진균 물질의 탐색 중에 Aspergillus candidus F1484 균주의 발효액으로부터 항진균 화합물 F1484를 단리하고 ethyl acetate 추출, silica gel column chromatography, ODS column chromatography 및 preparative HPLC를 행하여 분리정제하였다. F1484 화합물은 EI-MS, ^13C-, ^1H-NMR, DEPT, HMQC 및 HMBC에 의한 구조분석을 행한 결과 이 화합물은 항진균 물질인 chloroflavonin과 동일한 구조를 나타내었으며 효모형태의 Candida albicans에 대한 항진균 활성뿐만 아니라 여러 종류의 인체 종양 세포주에 대해서도 세포독성을 나타내었다. In the course of screening for the antifungal compounds against Candida albicans, an antifungal compound (F1480) was isolated from the culture broth of Aspergillus candidus F1484. Isolation and purification of compound F1484 were performed using ethyl acetate extraction, silica gel column chromatography, ODS column chromatography, and preparative HPLC. The structure of compound F1484 was determined by the spectroscopic analyses of EI-MS, ^13C-, ^1H-NMR, DEPT, HMQC, and HMBC. This compound appeared to have a structure of antifungal agent, chloroflavonin. In addition to antifungal activities against the yeast phase of Candida species, compound F1484 showed cytotoxic effect against various human tumor cell lines.