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Sathishkumar, Natarajan,Sathiyamoorthy, Subramaniyam,Ramya, Mathiyalagan,Yang, Dong-Uk,Lee, Hee Nyeong,Yang, Deok-Chun Informa Healthcare 2012 Journal of enzyme inhibition and medicinal chemist Vol.27 No.5
<P>Anti-apoptotic proteins such as BCL-2, BCL-XL and MCL-1 bind with pro-apoptotic proteins to induce apoptosis mechanism. BCL-2 family proteins are key regulators of apoptosis process. Over expression of these anti-apoptotic proteins lead to several cancers by preventing apoptosis. A number of studies revealed that ginseng derivatives reduce tumor growth. Ginseng, the most valuable medicinal herb found in eastern Asia belongs to Araliaceae family. In this study, docking simulations were performed for anti-apoptotic proteins with several ginsenosides from Panax ginseng. Our finding shows ginsenosides Rf, Rg1, Rg3 and Rh2 have more binding affinity with BCL-2, BCL-XL and MCL-1 and other ginsenosides also interact with each anti-apoptotic proteins. Therefore, ginseng derivatives represent a novel class of potent inhibitors and could be used for cancer chemotherapy.</P>
Sathishkumar Natarajan,Senthil Kumar Thamilarasan,Jong-In Park,Hee-Jeong Jung,Ill-Sup Nou 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Cabbage (Brassica oleracea) is one of the most important vegetable crops in the world. Yet, its sensitivity to cold stress, especially at the seedling stage, could limit the production. Until now, only, few studies about heritably durable cold tolerance were carried out in cabbage. Hence this study was done to characterize the transcriptome profiles of two cabbage genotypes with contrasting responses to cold stress using Illumina Hiseq short read (paired-end) sequencing technology. MicroRNAs (miRNAs) represent a class of short, non-coding, endogenous RNAs which play important roles in post-transcriptional regulation of gene expression. Thisstudy,wesoughttoprovideamorecomprehensivepredictionofB. oleracea cold responsive miRNAs based on high through put sequencing using two contrasting genotypes. The raw sequences were processed for removal of poor-quality and adaptor sequences. Then, the high quality unigenes (58,094) reads were applied for length filtering. Then, unigenes reads were used in a BLASTN search against of Rfam database and known miRNA database (miRBase 18.0) to removal of non-coding RNA’s and identifies conserved miRNA’s in B. oleracea. Further, novel reads were searched against B. oleracea genome. Their flanking sequences in the genome were used to predict their secondary structures, target prediction, and functional analysis. This is first report to identify novel miRNAs for cold stress through high throughput techniques. Our findings will provide an overview of potential miRNAs involved in cold stress, which may provide important clues on the function of miRNAs in from B. oleracea and other closely related Brassica species.
Lokeshkumar, Booupathy,Sathishkumar, Venkatachalam,Nandakumar, Natarajan,Rengarajan, Thamaraiselvan,Madankumar, Arumugam,Balasubramanian, Maruthaiveeran Periyasamy The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5
Colon cancer is considered as the precarious forms of cancer in many developed countries, with few to no symptoms; the tumor is often diagnosed in the later stages of cancer. Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs. The cellular and molecular activities show therapeutic progression that may reduce the risk of developing cancer by modulating the factors responsible for colon carcinogenesis. Colon cancer was induced with DMH with a dose of (20 mg/Kg/body weight) for 15 weeks by subcutaneous injection once in a week. Myrtenal treatment was started with (230 mg/Kg/body weight) by intragastric administration, one week prior to DMH induction and continued till the experimental period of 30 weeks. The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals. The Histopathological analysis of colon tissues well supported the biochemical alterations and inevitably proves the protective role of Myrtenal. Treatment with myrtenal to cancer bearing animals resulted in a remarkable increase in the inherent antioxidants and excellent modulation in the morphological and physiological nature of the colon tissue. It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.
Jewel Howlader,Yeji Hong,Sathishkumar Natarajan,Kanij Rukshana Sumi,Hoy-Taek Kim,Jong-In Park,Ill-Sup Nou 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2
Melon ( Cucumis melo L.), belonging to the Cucurbitaceae family, is cultivated worldwide and is highly valued for its fruitquality. However, this important crop is negatively aff ected by several races of powdery mildew (PM) fungus, Podosphaeraxanthii . Hence, exploration of PM race-specifi c resistant markers would be an eff ective strategy to develop race-specifi cmelon cultivars. Young leaves from four melon genotypes, including susceptible SCNU1154 and race-specifi c-resistantEdisto47, PMR5, and MR1 lines, were sequenced by next-generation sequencing, specifi cally whole-genome resequencing(WGR), to detect race-specifi c single nucleotide polymorphism (SNP) markers. Including putative resistance gene ( R -gene)SNPs, 168, 83, and 122 race 5-specifi c SNPs with the exact variation were identifi ed on PM-related chromosome 2, 5, and12, respectively, for distinguishing race-specifi c lines by comparing the WGR data in the C. melo genome. Based on proximityto the PM resistance quantitative trait loci (QTLs) in physical maps, 43, 37, and 48 SNPs were screened on chromosome2, 5, and 12, respectively. Using a derived cleaved amplifi ed polymorphic sequence (dCAPS) method, polymorphismswere only found in three SNPs (SNPR5_119, SNPR5_120, and SNPR5_121) out of 48 against race 5 on chromosome 12. Among these three, a putative R -gene, an NBS-LRR-type SNP, SNPR5_119, displayed similar genotypic and phenotypicvariation to race 5-specifi c susceptible (SCNU1154, PMR45, WMR29, and Edisto47) and resistant (PI414723, PMR5, andMR1) lines in C . melo except for PI124112. Importantly, two other intergenic SNPs, SNPR5_120 and SNPR5_121, showedgenotypic and phenotypic variation between susceptible and resistant lines tested. High-resolution melting (HRM) analysiswas used to further validate the same expression patterns of SNPR5_120 as a dCAPS method in all lines tested. Therefore,the identifi ed PM race 5-specifi c candidate markers described here might be useful for a marker-assisted selection breedingprogram in C. melo .