http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
벼 흰잎마름병의 신속하고 간편한 진단을 위한 Recombinase Polymerase Amplification 등온증폭법
김신화(Shinhwa Kim),이봉춘(Bong Choon Lee),김현주(Hyun Ju Kim),최수연(Soo Yeon Choi),서수좌(Su Jwa Seo),김상민(Sang-Min Kim) 한국식물병리학회 2020 식물병연구 Vol.26 No.4
Xanthomonas oryzae pv. oryzae는 벼 흰잎마름병을 일으키는 세균이며, 벼 흰잎마름병은 벼 주요 재배지에서 꾸준하게 발생하고 있다. 본 연구에서는 벼흰잎마름병균을 신속하고 간편하게 검출하기 위해 등온증폭법 중 하나인 recombinase polymerase amplification (RPA)를 적용하여 현장진단 등을 위한 유전자기반 진단법을 개발하였다. RPA법은 짧은 시간의 등온 조건에서 유전자 증폭이 가능하다는 장점이 있다. 본 연구에서개발한 벼 흰잎마름병 RPA 진단법은 39oC에서 5분간만 반응하면 목표유전자의 증폭이 이루어지므로 기존 진단법보다 신속하고 간편하며 우리나라에 존재하는 K1–K3a의 4종 레이스에모두 적용가능하며, DNA 추출 없이 식물체 잎의 즙액으로 증폭반응 수행이 가능하며 기존 Taq 기반 PCR보다 약 10배 검출민감도가 높고 고가의 thermal cycler 없이도 항온수조, 발열블록 혹은 체온을 이용한 증폭반응 수행이 가능하다. 벼흰잎마름병은 벼의 농작물재해보험 대상재해 병충해 중의 하나이므로과학적 진단결과가 요구되나, 일선 농촌지도 현장에서는 유전자기반 진단을 위한 장비, 기술 등의 부족으로 분자진단이 어려웠다. 본 연구에서 개발한 벼 흰잎마름병 RPA 진단을 활용한다면 병징 의심부위 마쇄액을 주형으로, 손으로 5분 동안 반응시키는 것만으로도 신속하고 간편하게 벼 흰잎마름병의 목표유전자 증폭산물을 형성하므로 벼 흰잎마름병 진단의 최일선에서 활용할 수 있을 것이다. Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis.
Colletotrichum sojae에 의한 땅콩 탄저병 발생 보고
김신화 ( Shinhwa Kim ),최수연 ( Soo Yeon Choi ),정현정 ( Hyunjung Chung ),최낙중 ( Nak Jung Choi ),서보윤 ( Bo Yoon Seo ),김상민 ( Sang-min Kim ) 한국균학회 2024 韓國菌學會誌 Vol.52 No.1
In August 2023, leaf spot disease was observed in peanuts in Cheongju-si, Korea. Leaf spots occurred at the leaf margins and the lesions gradually expanded. Diseased leaf areas were light or dark brown and irregular in shape. A fungal isolate was obtained from symptomatic leaf and cultured on potato dextrose agar (PDA) medium at 25℃. An isolate was identified as Colletotrichum sojae based on morphological characteristics and sequences of the internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase-1, actin, and β-tubulin genes. Pathogenicity tests were performed on peanut seedlings in a conidial suspension (1×106 conidia/mL). Lesions were observed on the peanut leaf 5 d after inoculation. The pathogen was re-isolated from the lesions of the inoculated leaves. To the best of our knowledge, this is the first report of anthracnose on peanut caused by C. sojae in Korea.
Lee, Sung Bae,Noh, Shinhwa,Yeom, Hye Duck,Kim, Hyunah,Kim, Wonkil,Kim, Yoon Suh,Bae, Hyunsu,Lee, Jun-Ho Kyung Hee Oriental Medicine Research Center, Kyung 2016 Oriental Pharmacy and Experimental Medicine Vol.16 No.4
Alisma Rhizomes is used as a diuretic, hypolipidemic, anti-diabetic and anti-inflammatory agent in traditional East-Asian medicine. In this study, we tested the effect of Alisma Rhizomes on the <TEX>${\alpha}3{\beta}4$</TEX> nicotinic acetylcholine (nACh) receptor channel current in Xenopus oocytes. The acetylcholine-induced inward peak current (<TEX>$I_{ACh}$</TEX>) was measured with the two-electrode voltage-clamp technique. This experiment shows that the <TEX>${\alpha}3{\beta}4$</TEX> nACh receptor cRNA injected into oocytes followed by co-application with Alisma Rhizomes inhibited <TEX>$I_{ACh}$</TEX> in a noncompetitive or voltage insensitive condition. The half maximal inhibitory concentration (<TEX>$IC_{50}$</TEX>) of Alisma Rhizomes was <TEX>$12.5{\pm}3.4{\mu}g/ml$</TEX> and the Vmax was <TEX>$55.4{\pm}4.7$</TEX>. Protostane-type triterpenoids are the main active ingredient of Alisma Rhizomes (Alisol A, Alisol B, Alisol B 23-acetate, Alisol C 23-acetate). The respective IC50 values of Alisol A, Alisol B, Alisol B 23-acetate, and Alisol C 23-acetate were <TEX>$1.7{\pm}0.1$</TEX>, <TEX>$2.8{\pm}0.5$</TEX>, <TEX>$2.6{\pm}0.7$</TEX> and <TEX>$3.5{\pm}0.3{\mu}M$</TEX> in the <TEX>${\alpha}3{\beta}4$</TEX> nACh receptor expressed in Xenopus oocytes. Altogether, our research shows that protostane-type triterpenoids may modulate the <TEX>${\alpha}3{\beta}4$</TEX> nACh receptors expressed in oocytes in a reversible, concentration dependent and non-competitive manner. Furthermore, this modulation of the nACh receptor activity by protostane-type triterpenoids could underlie the pharmaceutics actions of Alisma rhizome.
Hye Duck Yeom,Young-Min Kim,Sung Bae Lee,Shinhwa Noh,Sanung Eom,Hyunah Kim,Wonkil Kim,Jae Hwan Lee,Hyunsu Bae,Jun-Ho Lee,H. Bae,J. H. Lee 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.3
Alisma Rhizome is a known tradition medication, which has been used for its diuretic, hypolipidemic, anti-diabetic, and anti-inflammatory purposes for thousands of years. The primary compounds of Alisma Rhizome are protostane type triterpenes, such as Alisols A, B or C. We previously demonstrated that Alisol derivatives (Alisols A, B, and C) have inhibitory effects on 5-hydroxytryptamine 3A (5-HT3A) currents1. In this study, we tested the effects of a new triterpene, Alisol-F, on human 5-HT3A and α3β4 nicotinic acetylcholine (nACh) receptor channel currents by using Xenopus oocytes expressing these channels. Co-application of Alisol-F inhibited 5-HT3A and α3β4 nACh receptor-mediated inward peak currents. The inhibitory effect of Alisol-F on 5-HT and ACh-induced inward peak currents occurred in a reversible and concentration- dependent manner. The half maximal inhibitory concentrations (IC50) of Alisol-F were 79.4±11.0 and 21.2±6.0 μM for the 5-HT3A and α3β4 nACh receptors, respectively. In addition, the inhibition of I5-HT and IACh by Alisol-F occurred noncompetitive and voltage insensitive manner. Taken together, these results show that Alisol-F may regulate 5-HT3A and α3β4 nACh receptors channel expressed in Xenopus oocytes.