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Shanjie Wang,Kegong Chen,Ye Wang,Zeng Wang,Zhaoying Li,JunChen Guo,Jianfeng Chen,Wenhua Liu,Xiaohui Guo,Guangcan Yan,Chenchen Liang,Huai Yu,Shaohong Fang,Bo Yu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background Large-dose melatonin treatment in animal experiments was hardly translated into humans, which may explain the dilemma that the protective effects against myocardial injury in animal have been challenged by clinical trials. Ultrasound-targeted microbubble destruction (UTMD) has been considered a promising drug and gene delivery system to the target tissue. We aim to investigate whether cardiac gene delivery of melatonin receptor mediated by UTMD technology optimizes the efficacy of clinically equivalent dose of melatonin in sepsis-induced cardiomyopathy. Methods Melatonin and cardiac melatonin receptors in patients and rat models with lipopolysaccharide (LPS)- or cecal ligation and puncture (CLP)-induced sepsis were assessed. Rats received UTMD-mediated cardiac delivery of RORα/cationic microbubbles (CMBs) at 1, 3 and 5 days before CLP surgery. Echocardiography, histopathology and oxylipin metabolomics were assessed at 16–20 h after inducing fatal sepsis. Results We observed that patients with sepsis have lower serum melatonin than healthy controls, which was observed in the blood and hearts of Sprague–Dawley rat models with LPS- or CLP-induced sepsis. Notably, a mild dose (2.5 mg/kg) of intravenous melatonin did not substantially improve septic cardiomyopathy. We found decreased nuclear receptors RORα, not melatonin receptors MT1/2, under lethal sepsis that may weaken the potential benefits of a mild dose of melatonin treatment. In vivo, repeated UTMD-mediated cardiac delivery of RORα/CMBs exhibited favorable biosafety, efficiency and specificity, significantly strengthening the effects of a safe dose of melatonin on heart dysfunction and myocardial injury in septic rats. The cardiac delivery of RORα by UTMD technology and melatonin treatment improved mitochondrial dysfunction and oxylipin profiles, although there was no significant influence on systemic inflammation. Conclusions These findings provide new insights to explain the suboptimal effect of melatonin use in clinic and potential solutions to overcome the challenges. UTMD technology may be a promisingly interdisciplinary pattern against sepsis-induced cardiomyopathy.
Guo-Liang Wang,Shaohong Qu,Zhengjie Yuan,Yu Zhang,Guojuan Xu,Dongling Bi,Haiyan Qu,Xiaowei Zou,Xiaoqing Gao,Haihe Yang,Haiyan He,Xuli Wang,Jiandong Bao,Shimin Zuo,Xuebiao Pan,Bo Zhou 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Rice sheath blight (SB), caused by Rhizoctoniasolani (R. solani), is a major threat to rice productionworldwide. The molecular mechanisms of the SB resistancein rice are poorly understood. The transcriptomes of the SBresistantrice cultivar YSBR1 and the susceptible cultivarLemont were analyzed after R. solani infection. A total of7624 differentially expressed genes (DEGs) were identifiedat one or more timepoints in a cultivar. 5526 and 5618 DEGswere differentially expressed in Lemont and YSBR1,respectively. YSBR1 exhibited stronger and earlier transcriptionalresponse to R. solani than Lemont. Gene ontology enrichmentanalysis revealed that genes that encode cell wall-modifyingand glycosyl-degrading enzymes or anti-microbial proteinswere specifically induced in YSBR1 at 6 hpi. MapMananalysis revealed that more DEGs related with cell wall, β-glucanses, respiratory burst, phenylpropanoids and ligninwere highly induced by R. solani in YSBR1 than in Lemont. The results also showed that receptor-like kinases and jasmonicacid signaling may play important roles in host resistance toR. solani. This study highlights potential candidate genes andsignaling pathways involved in rice sheath resistance and canhelp to further clarify the mechanistic events underlyingresistance and susceptibility to R. solani.
Construction and Application of Efficient<i>Ac-Ds</i>Transposon Tagging Vectors in Rice
Qu, Shaohong,Jeon, Jong-Seong,Ouwerkerk, Pieter B.F.,Bellizzi, Maria,Leach, Jan,Ronald, Pamela,Wang, Guo-Liang Wiley (Blackwell Publishing) 2009 Journal of integrative plant biology Vol.51 No.11
<P>Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and (iii) suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element (HPT-Ds). We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community.</P>
Effect of water on Cu/Zn catalyst for hydrogenation of fatty methyl ester to fatty alcohol
Hui Huang,Chunling Fan,Shaohong Wang,Shujia Wang,Guiping Cao 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.6
The effect of water on Cu/Zn catalyst prepared by co-precipitation for hydrogenation of methyl laurate in a slurry phase was studied using a stirred autoclave reactor system. The catalysts were characterized by means of XRD, BET, H2-TPR, SEM and TEM. The results indicate that catalytic activity decreases with increased amount of water in methyl laurate. Correlating with the results from the above characterization, it is found that the main causes for the water deactivation of the Cu/Zn catalyst were the water occlusion of active catalyst sites by the low solubility of water in the substrate and the promotion of crystal growth, as well as the Cu/Zn catalyst agglomeration in the presence of water.
Hui Huang,Guiping Cao,Shaohong Wang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3
Deactivation of co-precipitated Cu/Zn catalyst caused by exposure to either alkylthiols or dialkyldisulfides in catalytic hydrogenation of dodecyl methyl ester to dodecanol is explored in a stirred batchreactor. Catalytic activity decrement is highly dependent on levels of poisons, types of thiols/disulfides,chain length of alkanes. XRD, EDS and XPS are employed to characterize the fresh and spent Cu/Zncatalysts. It is evident that sulfuric species prefer to attack zinc oxide, forming chemically adsorbed Zn-SR at low sulfur levels, and ZnS is formed in the highly reductive atmosphere before sulfuric speciesattacking copper (forming Cu7S4 or Cu31S16).
Guiping Cao,Hui Huang,Shaohong Wang 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.9
The effect of trimethyl phosphate on Cu/Zn catalyst prepared by co-precipitation method for hydrogenation of methyl laurate to dodecanol in a slurry phase was studied using a stirred autoclave reactor system. The catalysts were characterized by means of XRD, EDS, XPS, SEM and BET. The results indicated that catalytic activity decreased with the increased amount of trimethyl phosphate. Correlating with the results from the above characterization, it was found that the main cause for the catalyst deactivation was the trimethyl phosphate occlusion of active sites by the physical adsorption and BET surface area decrement.
Reduction of Graphene Oxide and its Effect on Square Resistance of Reduced Graphene Oxide Films
Zhaoxia Hou,Yin Zhou,Guangbin Li,Shaohong Wang,Meihan Wang,Xiaodan Hu,Siming Li 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.6
Graphite oxide was prepared via the modified Hummers’ method and graphene via chemical reduction. Deoxygenation efficiency of graphene oxide was compared among single reductants including sodium borohydride, hydrohalic acids, hydrazine hydrate, and vitamin C. Two-step reduction of graphene oxide was primarily studied. The reduced graphene oxide was characterized by XRD, TG, SEM, XPS, and Raman spectroscopy. Square resistance was measured as well. Results showed that films with single-step N2H4 reduction have the best transmittance and electrical conductivity with square resistance of ~5746 Ω/sq at 70% transmittance. This provided an experimental basis of using graphene for electronic device applications.
Hyun-Bi Kim,전종성,Jung-Il Cho,Nayeon Ryoo,Shaohong Qu,Guo-Liang Wang 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.1
The development of rapid and efficient strategies to gen-erate selectable marker-free transgenic plants could help increase the consumer acceptance of genetically modified (GM) plants. To produce marker-free transgenic plants without conditional treatment or the genetic crossing of offspring, we have developed a rapid and convenient DNA excision method mediated by the Cre/loxP recombination system under the control of a -46 minimal CaMV 35S promoter. The results of a transient expression assay showed that -46 minimal promoter::Cre recombinase (-46::Cre) can cause the loxP-specific excision of a selectable marker, thereby connecting the 35S promoter and -glucuronidase (GUS) reporter gene. Analysis of stable transgenic Arabidopsis plants indicated a positive correlation between loxP-specific DNA excision and GUS expression. PCR and DNA gel-blot analysis further revealed that nine of the 10 tested T1 transgenic lines carried both excised and non-excised constructs in their genomes. In the subsequent T2 generation plants, over 30% of the individuals for each line were marker-free plants harboring the excised construct only. These results demonstrate that the -46::Cre fusion construct can be efficiently and easily utilized for producing marker-free transgenic plants.