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Ahmad, Nazir,Ahmed, Iftikhar,Shahzad, Armghan,Khalid, Nauman,Mehboob, Farrakh,Ahad, Karam,Ali, Ghulam Muhammad The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at $750mgL^{-1}$ phenol. The strain showed complete degradation of $750mgL^{-1}$ phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of $28mgL^{-1}h^{-1}$. The doubling time was recorded approximately as $12.49h^{-1}$. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.
Shahzad Anwar,Ahmad Naseem,Anis Mohammad The Korean Society of Plant Biotechnology 2006 Plant molecular biology and biotechnology research Vol.8 No.1
An efficient protocol for in vitro multiple shoot bud induction and plant regeneration from mature green cotyledon derived callus tissues of Acacia sinuata has been developed. Callus formation occurs at all the concentrations of thidiazuron (TDZ) in Murashige and Skoog's (MS) medium, but 0.6 ${\mu}M$ proved to be the best with maximum callus formation frequency. Supplementation of TDZ in combination with indole-acetic acid (IAA) in MS media accelerates shoot bud organogenesis in differentiating callus tissues with 60-70% conversion of shoot buds into shoot Most efficient shoot organogenesis was recorded when TDZ induced calli were subcultured at different concentrations of 6-benzyla-denine (BA). Optimum shoot bud induction and plant regeneration from callus was achieved when 0.6 ${\mu}M$ (TDZ) induced calli were subcultured at 3.0 ${\mu}M$ (BA) where $16.6{\pm}0.74$ shoots/unit callus on obtained. Rooting in in vitro differentiated shoots was achieved when transferred to medium containing different concentration of indole-3-butyric acid (IBA) in full & half strength MS medium. The well rooted plantlets were hardened and transferred to net house with 90% survival rate.
Nazir Ahmad,Iftikhar Ahmed,Armghan Shahzad,Nauman Khalid,Farrakh Mehboob,Karam Ahad,Ghulam Muhammad Ali 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Phenol is a toxic pollutant found in effluent ofnumerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collectedfrom industrial waste and enriched in mineral salt medium (MSM)contained 300 mg/L phenol. The strain was identified based on16S rRNA gene analysis as Pseudomonas species and thephylogenetic analysis affiliated the strain with Pseudomonasmonteilii (AF064458) as the most closely related species. Phenoltolerance of the strain in MSM supplemented with variousconcentrations of phenol indicates that the strain NCCP-407 cangrow best at 750 mg L−1 phenol. The strain showed completedegradation of 750 mg L−1 phenol in 56 hours when supplementas a sole source of carbon and energy with the average degradationrate of 28mg L−1 h−1. The doubling time was recorded approximatelyas 12.49 h−1. The present study suggests that this strain is efficientin phenol degradation and can be used in treatment of wastewatercontaining phenol.
Naveed Shahzad Ali,Abdul Ghaffar,Asif Ali Qaiser,Muhammad Ahmad,Khurram 한국고분자학회 2021 폴리머 Vol.45 No.1
The recovery of solvents from the extracted oils using conventional thermal separation processes i.e. distillation or evaporation is often an energy-intensive process. To provide an energy-efficient alternative, an organic solvent nanofiltration thin-film composite membrane was prepared by depositing a thin layer of polyaniline (PANI), a selective layer, via interfacial polymerization on a crosslinked polyimide (XPI), a base ultrafiltration support membrane, to provide selective transport of constituents of a miscella. The membrane was characterized by microscopic, spectroscopic, and thermal analysis. The evaluation of the separation performance was carried out by a dead-end stirred cell using three vegetable oils. For sunflower, corn, and castor oil/n-hexane mixtures, the membrane resulted in a % rejection of 50, 56, and -44, respectively. A comparison of mass flux and rejection of corn and castor oils suggests that the PANI/XPI membranes showed a strong ion-exchange behavior significantly recognizing charge macromolecules due to the presence of charged emeraldine PANI.
Mubbashar Hassan,Sanan Raza,Ahmad Yar Qamar,Naveed Muhammad Ilyas,Mateen Abdul,Noman Muhammad,Sayed Aun Muhammad,Abid Hussain Shahzad 사단법인 한국동물생명공학회 2024 Journal of Animal Reproduction and Biotechnology Vol.39 No.1
Dystocia, a challenging condition in obstetrics, can arise from various causes, including fetal monsters with structural abnormalities. This case report presents a unique case of dystocia due to a fetal monster known as Perosomus Elumbis in a beetal breed goat from Pakistan. The 4-years-old pregnant doe presented with prolonged straining and failure to deliver the fetus after 8 hours of labor. Upon examination, the cervix was dilated, and only the forelimbs of the fetus were visible in the birth canal. The subsequent delivery involved the application of manual traction by using a dystocia kit, and the removal of edematous fluid from the legs. The monster fetus exhibited absence of hair growth, along with the absence of thoracic vertebrae. Two other fetuses were present, with one found dead and the other alive. Posttreatment involved fluid therapy, antibiotics, and supportive care for the doe. This case report sheds light on the occurrence of Perosomus Elumbis fetal monsters and their impact on dystocia in goat breeding. Understanding the underlying causes and implementing appropriate management strategies are crucial for successful outcomes in similar cases.
Cluster-Based Device Mobility Management in Named Data Networking for Vehicular Networks
Gohar, Moneeb,Khan, Naveed,Ahmad, Awais,Najam-Ul-Islam, Muhammad,Sarwar, Shahzad,Koh, Seok-Joo Hindawi Limited 2018 Mobile information systems Vol.2018 No.-
<P>Named data networking (NDN) is an emerging technology. It was designed to eliminate the dependency of IP addresses in the hourglass model. Mobility is a key concern of the modern Internet architecture, even though the NDN architecture has solved the consumer mobility. That is, the consumer can rerequest the desired data contents, while the producer mobility remains as an issue in the NDN architecture. This paper focuses on the issue of producer mobility and proposes the cluster-based device mobility management scheme, which uses the cluster heads to solve the producer mobility issue in NDN. In the proposed scheme, a cluster head has all information of its attached devices. A cluster head updates the routes, when a device moves to the new access router by sending all the attachment information. The proposed scheme is evaluated and compared with the existing scheme by using the <I>ndnSIM</I> simulation. From the results, we see that the proposed scheme can decrease the numbers of interest packets in the network, compared with the existing scheme.</P>
Mubbashar Hassan,Sanan Raza,Ahmad Yar Qamar,Muhammad Ilyas Naveed,Abdul Mateen,Muhammad Noman,Sayed Aun Muhammad,Abid Hussain Shahzad The Korean Society of Animal Reproduction and Biot 2024 한국동물생명공학회지 Vol.39 No.1
Dystocia, a challenging condition in obstetrics, can arise from various causes, including fetal monsters with structural abnormalities. This case report presents a unique case of dystocia due to a fetal monster known as Perosomus Elumbis in a beetal breed goat from Pakistan. The 4-years-old pregnant doe presented with prolonged straining and failure to deliver the fetus after 8 hours of labor. Upon examination, the cervix was dilated, and only the forelimbs of the fetus were visible in the birth canal. The subsequent delivery involved the application of manual traction by using a dystocia kit, and the removal of edematous fluid from the legs. The monster fetus exhibited absence of hair growth, along with the absence of thoracic vertebrae. Two other fetuses were present, with one found dead and the other alive. Posttreatment involved fluid therapy, antibiotics, and supportive care for the doe. This case report sheds light on the occurrence of Perosomus Elumbis fetal monsters and their impact on dystocia in goat breeding. Understanding the underlying causes and implementing appropriate management strategies are crucial for successful outcomes in similar cases.
One-stage Hip Reconstruction for Developmental Hip Dysplasia in Children over 8 Years of Age
Irfan Qadir,Saeed Ahmad,Atiq uz Zaman,Chirag Muhammad Khan,Shahzad Ahmad,Amer Aziz 대한고관절학회 2018 Hip and Pelvis Vol.30 No.4
Purpose: This study was performed to assess the clinical and radiological outcomes following one-stage hip reconstruction, consisting of open reduction femoral shortening and pelvic osteotomy, for neglected developmental dislocation of the hip (DDH). Materials and Methods: This is a retrospective analysis of 77 hips in 65 patients (46 females and 19 males; 12 had bilateral dislocations), operated at a Ghurki Trust Teaching Hospital in Pakistan between 2013 and 2015. The average age at surgery was 11.02±3.43 years. According to the Tönnis classification, there were 10, 14, 22, and 31 patients in grades 1, 2, 3, and 4, respectively. The pelvic procedure utilized in this study was triple osteotomy (47 hips) followed by double and Salter osteotomy (18 and 12 hips, respectively). Postoperative evaluations were conducted using the modified MacKay’s scoring system (functional outcomes) and Severin’s scoring method (radiological assessment). Results: Postoperatively, there were 38 (49.4%), 19 (24.7%), 14 (18.2%), and 6 (7.8%) hips in Severin grade I, II, III and IV, respectively. According to the modified McKay criteria, there were 22 hips (28.6%) in excellent condition, 44 (57.1%) in good condition, 9 (11.7%) in fair condition and 2 (2.6%) in poor condition. Both patients with poor outcomes had an unstable, painful hip with evidence of avascular necrosis of the femoral head. Conclusion: Based on the results presented here, we recommend the single stage procedure of open reduction, femoral shortening and pelvic osteotomy for treatment of DDH in older children with good to excellent functional and radiological outcomes.
Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay,
Zar, Mian Sahib,Shahid, Ahmad Ali,Shahzad, Muhammad Saqib,Shin, Kyoung-Jin,Lee, Hwan Young,Lee, Sang-Seob,Israr, Muhammad,Wiegand, Peter,Kulstein, Galina Wiley (Blackwell Publishing) 2018 Journal of forensic sciences Vol.63 No.6
Liaqat Ali,Muhammad Shahzad,Nasir Orakzai,Ihsanullah Khan,Mubashira Ahmad 대한비뇨의학회 2015 Investigative and Clinical Urology Vol.56 No.9
Purpose: To determine the efficacy of mitomycin C in reducing the recurrence of anterior urethral stricture after internal optical urethrotomy (IOU). Materials and Methods: This was a randomized controlled trial conducted in the Department of Urology at the Institute of Kidney Diseases Peshawar from March 2011 to December 2013. A total of 151 patients who completed the study were divided into two groups by the lottery method. Group A (cases) comprised 78 patients in whom mitomycin C 0.1% was injected submucosally in the stricture after conventional IOU. Group B (controls) comprised 73 patients in whom IOU only was performed. Self-clean intermittent catheterization was not offered in either group. All patients were regularly followed up for 18 months. Recurrence was diagnosed by use of retrograde urethrogram in all patients and flexible urethroscopy in selected cases. Data were collected on a structured pro forma sheet and were analyzed by SPSS. Results: The mean age of the patients in group A was 37.31±10.1 years and that in group B was 40.1±11.4 years. Recurrence of urethral stricture was recorded in 11 patients (14.1%) in group A and in 27 patients (36.9%) in group B (p=0.002). The mitomycin group also showed a delay in recurrence compared with the control group (p=0.002). Conclusions: Recurrence of urethral stricture is high after optical urethrotomy. Mitomycin C was found to be highly effective in preventing the recurrence of urethral stricture after IOU.