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Lee, Mihye,Paik, Sang Kyoo,Lee, Min-Jung,Kim, Yoon-Jung,Kim, Sungdae,Nahm, Minyeop,Oh, Soo-Jin,Kim, Hyun-Man,Yim, Jeongbin,Lee, C. Justin,Bae, Yong Chul,Lee, Seungbok Elsevier 2009 Developmental Biology Vol.330 No.2
<P><B>Abstract</B></P><P>Hereditary spastic paraplegia (HSP) is an inherited neurological disorder characterized by progressive spasticity and weakness of the lower extremities. The most common early-onset form of HSP is caused by mutations in the human gene that encodes the dynamin-family GTPase Atlastin-1 (Atl-1). Recently, loss of the <I>Drosophila</I> ortholog of Atl-1 (Atl) has been found to induce locomotor impairments from the earliest adult stages, suggesting the developmental role of atlastin-subfamily GTPases. Here, we provide evidence that Atl is required for normal growth of muscles and synapses at the neuromuscular junction (NMJ). Atl protein is highly expressed in larval body-wall muscles. Loss-of-function mutations in the <I>atl</I> gene reduce the size of muscles and increase the number of synaptic boutons. Rescue of these defects is accomplished by muscular, but not neuronal expression of Atl. Loss of Atl also disrupts ER and Golgi morphogenesis in muscles and reduces the synaptic levels of the scaffold proteins Dlg and α-spectrin. We also provide evidence that Atl functions with the microtubule-severing protein Spastin to disassemble microtubules in muscles. Finally, we demonstrate that the microtubule-destabilizing drug vinblastine alleviates synapse and muscle defects in <I>atl</I> mutants. Together, our results suggest that Atl controls synapse development and ER and Golgi morphogenesis by regulating microtubule stability.</P>
이승복 ( Lee¸ Seungbok ),김정호 ( Kim¸ Jeongho ),이미주 ( Lee¸ Meeju ),김석문 ( Kim¸ Seokmun ),이재하 ( Lee¸ Jaeha ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.2
최근 해수면 상승과 이상기후에 따른 파력 증가로 높은 중량의 대형 케이슨의 사용이 증가하는 추세이다. 다만 대형 케이슨의 인양 및 거치 시 비용은 많이 증가한다. 이에 체결장치를 통해 프리캐스트 블록을 결속시켜 방파구조물을 구성하는 시공법이 각광을 받고 있다. 본 연구에서는 이러한 블록식 방파구조물 내부에 해수소통 공간을 두어 파력저감 및 항내정온도 유지에 효과적인 케이슨 블록의 설계를 위해 블록을 연결하는 기둥식 체결부 구조성능을 평가하였다.
Nahm, Minyeop,Kim, Sungdae,Paik, Sang Kyoo,Lee, Mihye,Lee, Seongsoo,Lee, Zang Hee,Kim, Jaesang,Lee, Daekee,Bae, Yong Chul,Lee, Seungbok The Society 2010 The Journal of neuroscience Vol.30 No.24
<P>The bone morphogenetic protein (BMP) ligand Glass bottom boat (Gbb) acts as a retrograde growth signal at the Drosophila neuromuscular junction (NMJ). Endocytic regulation of presynaptic BMP receptors has been proposed to attenuate retrograde BMP signaling. However, it remains unknown whether the Gbb signal is also regulated by postsynaptic mechanisms. Here, we provide evidence that Drosophila Cdc42-interacting protein 4 (dCIP4) functions postsynaptically to inhibit synaptic growth. dCIP4 is localized postsynaptically at NMJs. dcip4 mutations lead to synaptic overgrowth and increased presynaptic phosphorylated mothers against decapentaplegic (Mad) levels, and these defects are rescued by muscle-specific expression of dCIP4. Biochemical and genetic analyses demonstrate that dCIP4 acts downstream of Cdc42 to activate the postsynaptic Wsp-Arp2/3 pathway. We also show that BMP signaling is necessary for synaptic overgrowth in larvae lacking postsynaptic dcip4 or wsp. Finally, dCIP4 and Wsp inhibit Gbb secretion. Thus, we propose that dCIP4 restrains synaptic growth by inhibiting postsynaptic Gbb secretion through the Wsp-Arp2/3 pathway.</P>
The effect of word imagery on priming effect under a preconscious condition: An fMRI study
Lee, Jong-Sun,Choi, Jeewook,Yoo, Jae Hyun,Kim, Minjung,Lee, Seungbok,Kim, Ji-Woong,Jeong, Bumseok WILEY-LISS, INC 2014 HUMAN BRAIN MAPPING Vol.35 No.9
<P>Semantic priming is affected by the degree of association and how readily a word is imagined. In the association effect, activity in the perisylvian structures including the bilateral inferior frontal gyrus, the left middle temporal gyrus, and the supramarginal gyrus was correlated. However, little is known about the brain regions related to the effect of imagery word under the preconscious condition. Forty word pairs for high (HA)-, low (LA)-, and nonassociation (NA), nonword (NW) conditions were presented. Each 40 association word pairs (HA and LA) included 20 high (HI) and 20 low (LI) imagery prime stimuli, using a visually presented lexical decision task. A trial consisted of 30 ms prime, 30 ms mask, 500 ms probe, and 2–8 s stimulus onset asynchrony. Brain activation was measured using functional magnetic resonance imaging during word discrimination. Behavioral data indicated that the shortest response time (RT) was given for HA words, followed by LA and NA, and NW showed the longest RT (<I>P</I> < 0.01). RT was faster in HI than LI within HA, but not LA conditions (<I>P</I> < 0.01). Functional neuroimaging showed that differential brain regions for high imagery (HI) and low imagery (LI) words within low prime-target word association were observed in the left precuneus, left posterior cingulate gyrus, and right cuneal cortex. The present findings demonstrate that the effect of the degree of imagery on semantic priming occurs during the early stage of language processing, indicating an “automatic imagery priming effect.” Our paradigm may be useful to explore semantic deficit related to imagery in various psychiatric disorders.</P>
MICAL-like Regulates Fasciclin II Membrane Cycling and Synaptic Development
Seungbok Lee,Minyeop Nahm,Sunyoung Park,이지혜 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.10
Fasciclin II (FasII), the Drosophila ortholog of neural cell adhesion molecule (NCAM), plays a critical role in synaptic stabilization and plasticity. Although this molecule undergoes constitutive cycling at the synaptic membrane, how its membrane trafficking is regulated to ensure proper synaptic development remains poorly understood. In a genetic screen, we recovered a mutation in Drosophila mical-like that displays an increase in bouton numbers and a decrease in FasII levels at the neuromuscular junction (NMJ). Similar phenotypes were induced by presynaptic, but not postsynaptic, knockdown of mical-like expression. FasII trafficking assays revealed that the recycling of internalized FasII molecules to the cell surface was significantly impaired in mical-like-knockdown cells. Importantly, this defect correlated with an enhancement of endosomal sorting of FasII to the lysosomal degradation pathway. Similarly, synaptic vesicle exocytosis was also impaired in mical-like mutants. Together, our results identify Mical-like as a novel regulator of synaptic growth and FasII endocytic recycling.
Lee, Seongsoo,Lee, Seungbok 이화여자대학교 세포신호전달연구센터 2006 고사리 세포신호전달 심포지움 Vol. No.8
Axon extension and guidance require a coordinated assembly of F-actin and microtubules and regulated translation in the growth cone. The molecular basis of how the translation of mRNAs encoding guidance proteins could be closely tied to the pace of cytoskeletal assembly is poorly understood. Previous studies showed that Short Stop(Shot) mediates direct interactions between F-actin and microtubules required for motor and sensory axon extension in the Drosophila embryo. Here, we provide biochemical and genetic evidence that Shot functions in neurons together with a novel translation inhibitor, Krasavietz(Kra), to steer longitudinally directed CNS axons away from the midline. Kra can bind directly to the C-terminal domain of Shot. shot and kra mutants have weak robo-like phenotypes and synergistically affect midline avoidance. Mutations in shot and kra dominantly enhance the frequency of midline crossovers in embryos heterozygous for slit. We demonstrate that Kra interacts with the translation machinery and inhibits translation in vitro. These data suggest that Shot-Kra interaction controls the translation of mRNAs encoding proteins necessary for the Slit/Robo response of the growth cone. We propose previously unknown role of Shot as a direct physical link between translational regulation and cytoskeleton reorganization.