누낭비강문합술(Dacryocystorhinostomy)을 이용한 비루관폐쇄 교정술식에 관한 고찰

백경식,강승우,오상윤,이민정,허원실 大韓顎顔面成形再建外科學會 1992 Maxillofacial Plastic Reconstructive Surgery Vol.14 No.4

Epiphora is overflow of tears due to obstruction of lacrimal duct. Dacryocystorhinostomy is the most common procedure to eliminate the epiphora secondary to complete or partial obstruction of the nasolacrimal duct. The procedure is to artificially create passage between lacrimal sac and nasal cavity. Especially, epiphora would be accompany often by nasolacrimal duct obstruction when trauma of ormaxillofacial area lead to nasal fracture, medial wall fracture of orbit. Therefore in this case there are many case to perform dacryocystorhinostomy because probing and tubing is difficult to resolve the epiphora. We performed 4 cases of dacryocystorhinostomy for adult nasolacrimal duct obstruction from May 1991 to October 1991. The results were very satisfactory in all the case. Epipora disappeared in all case.

Nuclear Factor I-C 결손 생쥐에서 상아모세포의 형태학적 특징

고승백,이창섭,이난영,이상호,김흥중,박주철 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

NFI-C K/O 생쥐에서는 상아모세포의 분화과정에 이상이 초래되어 상아질 형성에 이상이 생기고 치근 형성이 불완전하게 이루어지는 것으로 알려져 있으나 이에 대한 명확한 기전은 잘 알려져 있지 않다. 상아모세포가 분화하여 정상적으로 상아질을 형성하기 위해서 핵과 세포질이 극성을 띠고 잘 조직화되어야 하며, 이 과정에서 다양한 세포사이 결합장치들이 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 NFI-C K/O 생쥐에서 상아질 형성에 이상이 생기는 것이 상아포세포의 형태학적 변화와 세포사이 결합장치들이 기능을 하지 못한 결과에서 기인한 것인지 알아보기 위하여, NFI-C K/O 생쥐에서 발생한 비정상적인 상아모세포들을 광학 및 투과 전자현미경을 이용하여 형태학적으로 관찰하고, Zo-1과 occludin의 발현을 면역조직화학적으로 관찰하여 세포사이 결합장치들의 분포를 확인하여 다음과 같은 결과를 얻었다. 1. 광학현미경 소견에서 NFI-C K/O 생쥐의 전치부 상아모세포는 세포 극성이 상실되고, 여러 층으로 배열되어 있었으며 상아질에 많은 세포들이 함입된 것과 같은 비정상적인 상아질의 소견을 나타냈다. 반면에 NFI-C K/O 생쥐의 구치부 상아모세포는 치관부에서는 잘 조직화된 소견을 보였으나 치근 형성 부위에서는 세포 배열이 불규칙해지고 세포 극성이 상실되었다. 2. 투과 전자현미경 소견에서 NFI-C K/O 생쥐 전치부의 비정상적인 상아모세포는 둥근 형태로 세포 사이 간격이 넓으며 폐쇄연접과 같은 세포사이 결합장치들이 전혀 관찰되지 않았다. 3. Zo-1의 면역조직화학적 염색에서 NFI-C K/O 생쥐의 전치부 법랑모세포의 근위부와 원위부에서 ZO-1이 강하게 발현되었으나 비정상적인 상아모세포에서는 ZO-1의 발현을 관찰할 수 없었다. 4. Occludin의 면역조직화학적 염색에서 정상 생쥐의 전치부 상아모세포에서는 occludin의 발현이 관찰되었으나 NFI-C K/O 생쥐의 비정상적인 상아모세포에서는 occludin의 발현이 관찰되지 않았다. 이상의 결과를 종합하여 볼 때 NFI-C의 결손은 상아모세포의 분화 이상을 초래하고 비정상적으로 상아질을 형성하는 과정에 세포사이 결합장치의 상실과 같은 형태학적인 변화의 중요한 요소로 작용하는 것으로 생각된다. NFO-C null mice demonstrated aberrant odntoblast differentiation and thus abnormal dentin formation while other tissues/organs in the body, including ameloblasts, appear to be unaffected and normal. However, little is known about the mechanism of NFI-C function in odontoblast differentiation and dentin formation. Odontoblasts are tall, highly polarized cells that are responsible for formation and maintenance of the predentin and dentin. An indication of their polarity is the acquisition of specialized intercellular junctions. As predontoblasts differentiate into odontoblasts, they are joined and attached at the apical end by well developed terminal webs of cytoskeletal actins, and associated tight as well as adherent njunctions. In this study, in order to investigate if disruption of the NFI-C gene interferes with formation of a specific or other structural proteins of the intercellular junctions, we examined morphological charcteristic of the aberrant odontoblast in NFI-C null mice using light and electron microscope. In addition, we determined the expression of major structural proteins of intercellular junctions, ZO-1 and occludin, during the differentiation of odontoblasts using immunohitochemistry. The results were as follows: 1. In light microscopy, abnormal odontoblasts of incisors of the NFI-C null mice were round in shape, lost their polarity, and trapped in osteodentin-like mineralized tissue. Mutant molars have relatively normal crowns, but short and abnormal differentiating adontoblasts in root formation area. 2. Electron microscopy of abnormal odontoblasts revealed the dissociation of the round osteoblast-like cells, the loss of their cellular polarity, and the absence of an intercellular junctional complex known as the tight junctions. 3. A mutant incisor showed labeling for ZO-1 at the proximal and distal ends of secreting ameloblasts, while staining for ZO-1 was not observed in the abnormal odontoblasts. 4. A normal incisor showed immunoreactivity for occludin in the differentiating odontoblasts. However, staining for occludin was not observed in the abnormal odntoblasts of mutant incisor. These results suggest that NFI-C gene causes dissociation of odontoblast and thus abberant odontoblast differentiation and abnormal dentin formation by interfering with the formation of intercellular junctions.

Polysulfide 前處理에 의한 Cyanide 含有廢水의 活性汚泥處理

金承鶴,李武康,韓基白 慶星大學校 環境問題硏究所 1992 環境硏報 Vol.2 No.1

The objective of this study was performed in order to obtain the changing efficiency of SCN- according to and reaction time. For that reason, the polysulfide was added to the cyanide wastewater in a fixed weight ratio. Herewith the design parameter was determined for the synthetic wastewater containing 300㎎/ℓof SCN- and was compared substrate removal constant(k) with adsorption reaction constant(k₁k₂) by adsorption equilibrium eqtion. The changing rate of CN- to SCN- was found to be pH 10, pH 12 for 90∼95 percent within 3 hours. On other hand, was found to be pH 7 for 60∼65 perdent due to the Polysulfide precipitating. On the occasion of 10, after the reaction time proceeded 3 hours, pH of bulk solution was varied from 10 to 7. But practically changing of SCN concentration was not significant. Treatment of the synthetic wastewater containing 300㎎/ℓof SCN- at 20±1℃ with the Hydraulic retention tin of 6 to 18 and MLVSS concentration of 3050 to 3590㎎/ℓgave 94.2∼96.5 percent in substrate removal efficiencies as COD . The design parameters of k, Y, ke, Vmax and Km were determined to 0.0247 d-1, 0.2119, 0.0561 d-1, 4 d-1, and 153.96㎎/ℓ. In adsorption teste using acclimated sludge, adsorption removal rate was 0.5476㎎/㎎ MLVSS. day at 314㎎/ℓ initial SCN- concentration and was 0.3045㎎/㎎ MLVSS·day at 576㎎/ℓ of initial SCN- concentration. It was adapted adsorption equilibrium equation for substrate removal by the activated sludege. In the results, Freudlich's adsorption reaction constant, k₁ was 0.0273 day-1 and n₁was 0.840 and Katz's adsorption reaction constant, k₂ was 0.0287 day-1 and n₂was 0.839. These values were similar in comparison with the substrate removal constant(k=0.247).

High-frequency micromechanical resonators from aluminium–carbon nanotube nanolaminates

Bak, Jung Hoon,Kim, Young Duck,Hong, Seung Sae,Lee, Byung Yang,Lee, Seung Ran,Jang, Jae Hyuck,Kim, Miyoung,Char, Kookrin,Hong, Seunghun,Park, Yun Daniel Nature Publishing Group 2008 NATURE MATERIALS Vol.7 No.6

At micro- and nanoscales, materials with high Young’s moduli and low densities are of great interest for high-frequency micromechanical resonator devices. Incorporating carbon nanotubes (CNTs), with their unmatched properties, has added functionality to many man-made composites. We report on the fabrication of ≤100-nm-thick laminates by sputter-deposition of aluminium onto a two-dimensional single-walled CNT network. These nanolaminates—composed of Al, its native oxide Al<SUB>2</SUB>O<SUB>3</SUB> and CNTs—are fashioned, in a scalable manner, into suspended doubly clamped micromechanical beams. Dynamic flexural measurements show marked increases in resonant frequencies for nanolaminates with Al–CNT laminae. Such increases, further supported by quasi-static flexural measurements, are partly attributable to enhancements in elastic properties arising from the addition of CNTs. As a consequence, these nanolaminate micromechanical resonators show significant suppression of mechanical nonlinearity and enhanced strength, both of which are advantageous for practical applications and analogous to biological nanocomposites, similarly composed of high-aspect-ratio, mechanically superior mineral platelets in a soft protein matrix.

Epitaxial Growth of Crack-Free GaN on Patterned Si(111) Substrate

Lee, Seung-Jae,Bak, Gyu Hyeong,Jeon, Seong-Ran,Lee, Sang Hern,Kim, Sang-Mook,Jung, Sung Hoon,Lee, Cheul-Ro,Lee, In-Hwan,Leem, Shi-Jong,Baek, Jong Hyeob IOP Publishing 2008 Japanese journal of applied physics Vol.47 No.4

Inhibitory Effects of Isorhamnetin-3-<i>O</i>-β-<small>D</small>-glucoside from <i>Salicornia herbacea</i> on Rat Lens Aldose Reductase and Sorbitol Accumulation in Streptozotocin-Induced Diabetic Rat Tissues

Lee, Yeon Sil,Lee, Sanghyun,Lee, Hye Seung,Kim, Bak-Kwang,Ohuchi, Kazuo,Shin, Kuk Hyun Pharmaceutical Society of Japan 2005 Biological & pharmaceutical bulletin Vol.28 No.5

<P>The inhibitory effects of compounds from <I>Salicornia herbacea</I> (Chenopodiaceae) on rat lens aldose reductase (RLAR) and sorbitol accumulation in streptozotocin-induced diabetic rat tissues were investigated. The various fractions from the MeOH extract of <I>S. herbacea</I> were tested for their effects on RLAR <I>in vitro</I>. Among them, the EtOAc fraction was found to exhibit a potent RLAR inhibition (IC<SUB>50</SUB>=0.75 μg/ml), from which an active principle as a potent AR inhibitor was isolated and its chemical structure was elucidated as isorhamnetin-3-<I>O</I>-β-<SMALL>D</SMALL>-glucoside (1) by spectral analysis. Compound 1 exhibited a potent RLAR inhibition <I>in vitro</I>, its IC<SUB>50</SUB> being 1.4 μ<SMALL>M</SMALL>. Compound 1, when administered orally at 25 mg/kg in streptozotocin (STZ)-induced diabetic rats, caused not only a significant inhibition of serum glucose concentration but also sorbitol accumulation in the lenses, red blood cells (RBC), and sciatic nerves. These results indicate that compound 1 from <I>S. herbacea</I> is a leading compound for further study as a new drug for the prevention and/or treatment of diabetes and its complications.</P>

Single Nodular Pulmonary Amyloidosis: Case Report

Lee, Seung Hyun,Ko, Young Chun,Jeong, Jong Pil,Park, Chan Woo,Seo, Seok Ho,Kim, Jong Taek,Park, Dae Won,Bak, Cheol Min,Moon, Seung Ki,Jo, Shin Hyoung,Kim, Se Mi,Jung, Ah Lon The Korean Academy of Tuberculosis and Respiratory 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4

Amyloidosis is defined as the presence of extra-cellular deposits of an insoluble fibrillar protein, amyloid. The pulmonary involvement of amyloidosis is usually classified as tracheobronchial, parenchymal nodular, or diffuse alveolar septal. A single nodular lesion can mimic various conditions, including malignancy, pulmonary tuberculosis, and fungal infection. To date, only one case of nodular pulmonary amyloidosis has been reported in Korea, a case involving multiple nodular lesions. Here, we report and discuss the case of a patient having single nodular amyloidosis.

CASE REPORT : Single Nodular Pulmonary Amyloidosis: Case Report

( Seung Hyun Lee ),( Young Chun Ko ),( Jong Pil Jeong ),( Chan Woo Park ),( Seok Ho Seo ),( Jong Taek Kim ),( Dae Won Park ),( Cheol Min Bak ),( Seung Ki Moon ),( Shin Hyoung Jo ),( Se Mi Kim ),( Ah L 대한결핵 및 호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4

Amyloidosis is defined as the presence of extra-cellular deposits of an insoluble fibrillar protein, amyloid. The pulmonary involvement of amyloidosis is usually classified as tracheobronchial, parenchymal nodular, or diffuse alveolar septal. A single nodular lesion can mimic various conditions, including malignancy, pulmonary tuberculosis, and fungal infection. To date, only one case of nodular pulmonary amyloidosis has been reported in Korea, a case involving multiple nodular lesions. Here, we report and discuss the case of a patient having single nodular amyloidosis.

Constituents of the Halophyte Salicornia herbacea

Lee, Yeon-Sil,Lee, Hye-Seung,Shin, Kuk-Hyun,Kim, Bak-Kwang,Lee , Sang-Hyun The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.10

Four compounds were isolated from Salicornia herbacea by repeated column chromatography. Their structures were identified as ${\beta}$-sitosterol (1), stigmasterol (2), uracil (3), and isorhamnetin-3-O-${\beta}$-D-glucopyranoside (4) by spectral analysis and comparison with the published data.

Altered epidermal lipid layers induced by long‐term exposure to suberythemal‐dose ultraviolet

Bak, Hana,Hong, Seung‐,phil,Jeong, Se‐,Kyoo,Choi, Eung‐,Ho,Lee, Sang E.,Lee, Seung‐,Hun,Ahn, Sung‐,Ku Blackwell Publishing Ltd 2011 International journal of dermatology Vol.50 No.7

<P><B>Abstract</B></P><P><B>Background </B> Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long‐term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime.</P><P><B>Objectives </B> To investigate the changes of epidermal lipid properties after long‐term UV radiation with SED.</P><P><B>Materials and methods </B> Hairless mice were irradiated three times weekly for 15 weeks at an SED of UV (UVB: 20 mJ/cm<SUP>2</SUP>; UVA: 14 J/cm<SUP>2</SUP>). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase‐polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high‐performance thin‐layer chromatography with exposed times.</P><P><B>Results </B> The SED UV‐irradiated group showed increased TEWL after 12 weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide.</P><P><B>Conclusions </B> Long‐term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.</P>