殺蟲性 O,O-Diethylphenylphosphate 誘導體의 形態와 反應性에 關한 分子軌道論的 硏究

朴勝熙,成洛道,明平根,全容求,李天培 충남대학교 약학대학 의약품개발연구소 1985 藥學論文集 Vol.1 No.-

Molecular orbital theoretical study on the stability of conformations and chemical reactivity of 0,0-diethyphenylphosphate derivatives were carried out by EHT and CNDO/2 molecular orbital calculation method. The results shown that the dipole moment(μ) and total energies of the θ=90° conformer were μ=3.185D & E_t=-162.6479(au) and also that of the θ=0° conformer were μ=5.596D & E_t=-162.4013(au), respectively. Therefore, the values of μ & E_t of the θ=90° conformer were much smaller than that of the θ=0° conformer. The form with angle of rotation θ=90° of phenyl ring was shown to be most stable and this was interpreted in terms of electrostatic and steric effect. O,O-diethylphenylphosphate derivatives are predicted to increase both charge and orbital controlled SN_2 reactivity of the electron withdrawing substituent reduces the HOMO & LUMO energy, while the electron withdrawing substituent due to increase in positive charge of phosphorus atom of phosphate molecule.

AHP를 이용한 소프트웨어 외주업체 선정방안에 관한 연구

김승렬,전희숙 한국경영과학회 1995 經營 科學 Vol.12 No.2

The objectives of this paper are to provide software developer selection criteria and to develop evaluation framework using AHP(Analytic Hierarchy Process). The selection criteria are extracted from Software Development Life Cycle, Quality Assurance, and Productivity of Organization. In this paper, the selection model is proposed and its examples are illustrated. Though some further research is required, the proposed model can be regarded as a basis of a DSS for the selection of the software developer.

Comet Assay 를 이용한 제주 자생 녹조류 추출물의 세포내 DNA 손상 억제 활성 효과

안긴내,이승홍,차선희,고석천,전유진 제주대학교 방사능이용연구소 2006 방사선응용과학연구소논문집 Vol.20 No.-

Isolation and Phylogeny of HERV-W pol Fragments in Human monochromosomes

Jeon,Seung Heui,Yi,Joo Mi,Shin,Kyung Mi,Lee,Ji Won,Hong,Kyung Won,Huh,Jae Won,Jung,A Ram,Choi,Jin,Lee,Won Ho,Kim,Heui Soo 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.33 No.-

A new human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Using the PCR approach with human monochromosomal somatic cell hybrid DNA panel, twenty-four pol fragments of HERV-W family from chromosomes 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 20, 21, X, and Y were identified and analyzed. They showed a high degree of nucleotide sequence similarity (89.3-91.3%) with that of the HERV-W. Translation of the pol fragments showed no frameshift and termination codon by deletion/insertion or point mutation in some clones, HWP4-1; HWP4-2 from chromosome 4, and HWPX-1 from chromosome X. Phylogenetic analysis from the HERV-W family indicates that the pol fragments has evolved independently among chromosomes or represent integration events separately during primate evolution.

Positive feedback regulation of Akt‐FMRP pathway protects neurons from cell death

Jeon, Se Jin,Han, Seol‐,Heui,Yang, Sung‐,Il,Choi, Ji woong,Kwon, Kyoung Ja,Park, Seung Hwa,Kim, Hahn Young,Cheong, Jae Hoon,Ryu, Jong Hoon,Ko, Kwang Ho,Wells, David G,Shin, Chan Young Blackwell Publishing Ltd 2012 Journal of Neurochemistry Vol.123 No.2

<P><I>J. Neurochem.</I> (2012) <B>123</B>, 226–238.</P><P><B>Abstract</B></P><P>Fragile X syndrome (FXS), the most common single genetic cause of mental retardation and autistic spectrum disease, occurs when <I>FMR1</I> gene is mutated. <I>FMR1</I> encodes fragile X mental retardation protein (FMRP) which regulates translation of mRNAs playing important roles in the development of neurons as well as formation and maintenance of synapses. To examine whether FMRP regulates cell viability, we induced apoptosis in rat primary cortical neurons with glutamate <I>in vitro</I> and with middle cerebral artery occlusion (MCAO) in striatal neurons <I>in vivo</I>. Both conditions elicited a rapid, but transient FMRP expression in neurons. This up‐regulated FMRP expression was abolished by pre‐treatment with PI3K and Protein Kinase B (Akt) inhibitors: LY294002, Akt inhibitor IV, and VIII. Reduced FMRP expression <I>in vitro</I> or <I>in vivo</I> using small hairpin <I>Fmr1</I> virus exacerbated cell death by glutamate or MCAO, presumably <I>via</I> hypophosphorylation of Akt and reduced expression of B‐cell lymphoma‐extra large (Bcl‐xL). However, over‐expression of FMRP using enhanced green fluorescent protein (eGFP)‐FMRP constructs alleviated cell death, increased Akt activity, and enhanced Bcl‐xL production. The pro‐survival role of Akt‐dependent up‐regulation of FMRP in glutamate‐stimulated cultured neuron as well as in ischemic brain may have a clinical importance in FXS as well as in neurodegenerative disorders and traumatic brain injury.</P>

Molecular Phylogeny of Endogenous Retrovirus HERV-F Family in Japanese and Rhesus Monkeys

Kim, Heui-Soo,Jeon, Seung Heui,Yi, Joo-Mi,Kim, Tae-Hyeong,Kim, Myung-Sook,Hyun, Byung-Hwa,Takenaka, Osamu 한국유전학회 2002 Genes & Genomics Vol.24 No.2

A new human endogenous retroviral family (HERV-F) has recently been identified from the human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 probe. The current study investigated pol fragments of the HERV-F family from Japanese and rhesus monkeys and compared them with those of the HERV-F (Hu-XA34) family. Fourteen pol fragments of the HERV-F family were detected from the monkeys, which showed a 78.7-95.4% sequence similarity with those of HERV-F (Hu-XA34). Clones FJM-1, FJM-7, FJM-14, and FJM-15 from the Japanese monkey and FRH-1 and FRH-4 from the rhesus monkey exhibited no disruption due to point mutation or insertions/deletions. The ratio of synonymous to non-synonymous substitutions indicated that negative selective pressure was acting on these clones. Therefore, the pol gene sequences could be associated with an active provirus in the monkey genomes. A phylogenetic analysis of the pol fragments from humans and monkeys using the neighbor joining and maximum parsimony methods showed six groups, indicating that either the HERV-F family was amplified at least six times after its original integration into the monkey genome or the occurrence of independent integration events during primate evolution.

Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain

Kim, Heui-Soo,Jeon, Seung-Heui,Yi, Joo-Mi,Kim, Tae-Hyung,Lee, Won-Ho Korean Society of Life Science 2003 생명과학회지 Vol.13 No.3

인간 내생 레트로바이러스 HERV-W는 다발성 경화증 환자로부터 탐지된 MSRV와 연루되어 있다. 인간 태아의 뇌로부터 유래된 cDNA library를 이용하여 PCR법으로 2개의 HERV-W 패밀리(HWP-FB10과 HWP-FB12)를 동정하고 분석하였다. 그들은 HERV-W (accession no. AF009668)와 89%의 염기서열의 유사성을 보였다. Pol 유전자를 아미노산의 서열로 분석해 본 결과 점돌연변이 또는 삽입/결실로 말미암아 frameshift 및 종결코돈을 나타내었다. 유전자정보의 데이터베이스를 이용하여 HERV-W 패밀리간의 분자계통분류도를 작성해 본 결과 HWP-FB10은 인간의 염색체 7q21-22로부터 유래된 AC000064와 매우 가깝게 관련되어 있음을 시사하였다. 이들의 새로운 HERV-W pol 패밀리가 이웃하는 어떤 유전자와 상호 연결되어 있으며, 어떠한 기능을 수행하는지에 대한 전망에 대해 토의하였다. A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.

Long Terminal Repeat of an Endogenous Retrovirus HERV-K Family from Human Liver and Kidney cDNA

Kim, Heui-Soo,Choi, Joo-Young,Lee, Joo-Mi,Jeon, Seung-Heui,Lee, Young-Choon,Lee, Won-Ho,Jang, Kyung-Lib Korean Society of Life Science 2000 Journal of Life Science Vol.10 No.2

Long terminal repeat (LTR) of human endogenous retrovirus K family (HERV-K) has been found to be coexpressed with sequences of closely located genes. We examined the transcribed HERV-K LTR elements in human liver and kidney tissues. Using the cDNA synthesized from mRNA of human liver and kidney, we performed PCR amplification and identified six HERV-K LTR elements. Those LTR elements showed a high degree of sequence similarity (93.3∼96.6%) with human-specific LTR. A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-K LTR elements (Liv-1, 2, 3 and Kid-1, 2, 3) were belonged to group I. Our data suggests that HERV-K LTR elements are active on human liver and kidney tissues and may represent a source of genetic variation connected to human disease.

AO Tension Band Wiring방법을 이용한 견봉쇄골관절 탈구의 치료

박희전(Heui Jeon Park),윤여승(Yeu Seung Yoon),이정모(Jung Mo Lee) 대한정형외과학회 1987 대한정형외과학회지 Vol.22 No.1

Creep 모델을 이용한 Ground Anchor의 거동해석

전승철(Seung-Chul Jeon),한희수(Heui-Soo Han) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.12

본 논문은 앵커의 리프트 오프 시험(Lift –off Test) 및 앵커 단기 크립(creep) 시험결과를 이용하여 비탈면 앵커의 성능을 분석한 것이다. 이를 위해, 앵커 리프트 오프 시험 시 하중~변위곡선과 앵커의 탄성곡선을 분석하여 앵커의 성능을 분석하였으며, 단기크립시험에서 구한 변위-시간 곡선으로, 크립 계수를 구하고 크립 발생여부를 판단하였다. 또한, 추후 잔존 긴장력 감소 여부를 판단하기 위해, Rheology model과 쌍곡선 모델을 이용한 장기 크립거동 해석을 하였다. 단기 크립의 경우, 크립 계수값이 1mm 이하면 크립이 발생하지 않는 것으로 간주하고 1mm를 초과하면 크립 가능성이 있으므로 별도 크립 시험을 실시한다. 그러나 앵커의 장기 크립 거동에 대한 시방기준은 현재 제시된 방법이 없어, 본 논문은 이에 대한 기준을 제시하고자 하였다. 본 논문을 위해, 현장의 6개의 앵커(Ⓐ~Ⓕ앵커)에 대하여 단기 크립 시험을 행하였으며, 이 결과를 이용하여 앵커의 ①잔존 긴장력, ②탄성변위 및 ③크립거동을 해석하였다. 본 논문에서 단기 크립 시험 결과를 이용하여 레올로지모델 및 쌍곡선 모델로 장기 크립거동 분석한 결과, 단기 크립 시험결과로, 장기 크립 거동에 대하여서는 정확한 판단을 내릴 수 없었다. 그러므로, 장기 크립 거동에 대한 별도 분석이 필요하다. 앵커의 장기 잔존긴장력 및 비탈면의 장기 안정성 해석을 위해서는 앵커의 장기 크립거동 해석 및 최종 크립량 계산이 필요하며, 분석모델로는 쌍곡선 모델을 제안한다. This paper analyzes the performance of a slope anchor using the anchor lift-off test and the anchor short-term creep test results. As part of this analysis, the anchor"s performance was studied by analyzing the load-displacement curve and the anchor"s elasticity curve from the anchor lift-off test. A long-term creep analysis using a rheology model and a hyperbolic model was performed to check if the residual tension will reduce in the future. In the case of short-term creep, if the creep coefficient value is 1 mm or less, then creep does not occur. On the other hand, if the creep coefficient exceeds 1 mm, a separate creep test has to be conducted However, there is currently no specific method to test the long-term creep behavior of anchors. So, this research presents a standard for the long-term creep testing of anchors. At first, short-term creep tests were conducted on six anchors (Ⓐ~Ⓕ anchors), and using these results, ① residual tension, ② elastic displacement, and ③ creep behavior were analyzed. In any case, it was not possible to make an accurate judgment of the long-term creep behavior with these short-term creep test results. However, to meet the said goals of the research, a hyperbolic model is proposed to analyze the long-term residual tension of the anchor and the long-term stability of the slope.