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차선희,차선희,이정섭,이주상,석진원 (사)한국해양바이오학회 2023 한국해양바이오학회지 Vol.15 No.2
This study aimed to develop low-salt seasoned Gim (김) with new functionality to meet the diversification of the export market and consumer demand for Gim, which is one of the four major seafood products in Chungcheongnam-do province. Spirulina, a microalgae containing essential amino acids, vitamins, and minerals, is known to have antioxidant components such as phycocyanin. Therefore, to determine the suitability of spirulina as a seasoning material, we conducted seaweed. It can be used as a substitute for seasoning salt in the development of low-salt seasoned laver with enhanced functionality.
차선희,이기완,전유진,Cha, Seon-Heui,Lee, Ki-Wan,Jeon, You-Jin The Korean Society of Phycology 2006 ALGAE Vol.21 No.3
This study was conducted to screen in vitro angiotensin - I converting enzyme (ACE) inhibitory activities of methanol (MeOH) and aqueous extracts at 20°C and 70°C, respectively, prepared from twenty-six red algae obtained from the coast of Jeju Island in Korea. Among aqueous extracts at 20°C (20AE) from red algae Lomentaria catenata showed the strongest ACE inhibitory activity and Lithophyllum okamurae recorded the second highest activity. From MeOH extract at 20°C (20ME) Ahnfeltiopsis flabelliformis possessed the strongest ACE inhibitory activity. Remarkable activities from MeOH extracts at 70°C (70ME) were observed in Grateloupia filicina, Sinkoraena lancifolia and Grateloupia lanceolata. However, no significant activity was found in aqueous extracts at 70°C (70AE). The IC50 values, which are concentrations required to inhibit 50% activity of ACE, for ACE inhibitory activities of 20AE from Lithophyllum okamurae and L. catenata were 13.78 and 12.21 μg mL–1, respectively. The IC50 values of 20ME from A. flabelliformis and Laurencia okamurae were 13.84 and 106.15 μg mL–1. Those of the 70ME from Bonnemaisonia hamifera, Grateloupia filicina, Sinkoraena lancifolia, G. lanceolata, Gracilaria vermiculophylla and L. okamurae ranged from 25.82 to 124.69 μg mL–1.
Electron Spin Resonance을 이용한 제주 자생 해조류의 DPPH Free Radical 소거활성 검색
차선희,허수진,전유진,Cha, Seon-Heui,Heo, Soo-Jin,Jeon, You-Jin 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.4
본 실험은 electron spin/paramagnetic resonance(ESR/EPR)를 이용하여 기존의 DPPH free radical 소거 활성을 측정하였고, 이를 해조류 유래 천연자원으로부터 새로운 항산화물질을 탐색하는데 적용해 보았다. 연구 결과, 녹조류 추출물에서는 참홑파래, 납작파래 및 청각의 고온 메탄올 추출물에서만 약간의 라디칼 소거활성을 나타내었고, 이 외의 추출물에서는 소거활성이 거의 보이지 않았다. 갈조류는 총 71개의 추출물 중 37개의 추출물에서 80% 이상의 소거율을 보여 아주 우수한 항산화 물질을 가진 family로 여겨지며, 특히 모자반류 (Sargassum spp.)는 수용성과 메탄올, 고온과 상온의 모든 추출물에서 높은 라디칼 소거 활성을 보여 앞으로의 신소재 물질 후보로 선별되었다. 홍조류에서는 수용성보다는 메탄올 추출물에서 대체적으로 높은 소거활성이 검색되어, 나중에는 유기용매 분획물을 제조하여 라디칼 소거능을 측정 할 수 있을 것이다. 이처럼 해조류는 라키칼 소거 능력을 가지는 성분을 여러 형태로 다량으로 함유 하고 있는 것으로 사료 되며 앞으로의 연구에 기대가치를 더해 줄 것으로 사료된다. Extracts which were prepared by four different extractions - 80% methanol extracts (ME) at high ($70^{\circ}C$) and a room temperature ($20^{\circ}C$), respectively and aqueous extracts (AE) at both temperatures with the residue after the methanol extracts - of 10 green, 19 brown and 25 red seaweeds collected in Jeju Island coast were examined for their DPPH free radical scavenging activity using a ESR (electron spin resonance) spectroscopy. A variety of the extracts showed positive scavenging effect against DPPH free radical (except the green seaweeds). Among the extracts, the brown seaweed extracts exhibited the highest scavenging activity. Especially, Sargassum spp. of the brown seaweeds have remarkable scavenging activities - both methanolic and aqueous at the both temperatures ($20^{\circ}C$ and $70^{\circ}C$). On the other hand, ME showed better scavenging activity than AE in the red seaweed extracts. These results indicate that autogenous seaweeds in Jeju will be potential natural antioxidants for functional food compounds.
40세이상의 여성에서 난관복원술 후 임신율에 관한 임상적 고찰
차선희 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.2
1989년 1월부터 1994년 4월까지 만 5년 4개월간 차병원 산부인과에서 난관복원술을 시행한 40세이상의 여성 60예중 1년 이상 추적검사가 가능했던 43예를 대상으로 다음과 같은 결론을 얻었다. 1. 대상환자의 연령은 평균 41.1세(40-45세)이고, 같은 시기에 시행한 764예의 5.6%에 해당된다. 2. 복원수술 후 임신까지의 평균 경과기간은 7.9개월(1-19개월)이었다. 3. 1년이상 추적조사가 가능했던 43명 중 25명에서 임신이 확인되어, 58.1%의 임신성공율을 보였는데, 그 구체적인 내용은 만삭분만 46.5%, 자연유산 28%, 난관임신 2.3%였다. 4. 불임시술 방법에 따른 임신성공율은 복강경에 의한 ring 삽입의 경우 73.3%, 전기소작술의 경우 45.8%로 양 방법간에 차이가 있음을 볼 수 있었다. 5. 연령에 따른 임신율은 40세의 경우 65%, 41세의 경우 100%, 42세의 경우 57.1%로 나타나 연령에 따른 임신율의 차이는 뚜렷하게 나타나지 않았다. During the 5 year period from Jan. 1989 to Apr. 1994, the women who underwent reversal of sterilization at an age of $gt; 40 years were analyzed. During the study period, a total of women $gt; age 40 underwent reversal of sterilization and 43 cases of them were followed up for more than 1 year postoperatively in the department of obstetrics and gynecology, CHA General Hospital. The results are as follows: 1. The mean age was 41.1 and these 43 procedures amounted to 5.6% of the total number of sterilization reversals(n=764) during the same period in the CHA General Hospital. 2. The mean duration between the operation and the occurrence of the first pregnancy was 7.9 months(range 1 to 19 months). 3. The overall pregnancy rate after tubal reversal was 58.1% and the outcome of the pregnancy was as follows: term pregnancy 46.5%, spontaneous abortion 28%, and ectopic pregnancy 2.3%. 4. According to the method of preceding sterilization, a distinct difference of pregnancy rates in favor of ring sterilization was observed: a 73.3% intraouterine pregnancy rate was achieved after this procedure.
차선희,안희정,이정노,권태희,박용현 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.6
저자들은 7예의 태반 혈관종을 산전 초음파검사에서 진단하였고 병리학적으로 확진되었기에 문헌 고찰과 함 께 보고하는 바이다. Since the placenta is an organ composed of blood vessels, it is not surprising that its primary neoplasm would be a vascular tumor. Placental tumors, primary or secondary, have been known to interfere with placental function. Chorioangioma(primary tumor of the placenta), which is the most common of them, occurs with an incidence for clinically significant ranges from 1∼2.8:10000 births. These tumors are benign and are not usually associated with clinical sequelae unless they are larger than 5cm in long diameter. About one third of the large chorioangiomas may be associated with the maternal and fetal complications. For diagnosis of these lesions, the ultrasonography was used. If the chorioangioma is suspected, color doppler study is informative to confirm the presence of the vascular channels. We reviewed ultrasonograms and clinical records of seven patients who had been diagnosed as placental chrioangioma. The appropriate diagnostic tests and treatment can then be initiated in order to prolong gestation and decrease fetal mortality and morbidity.