http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
&agr;-Synuclein induces migration of BV-2 microglial cells by up-regulation of CD44 and MT1-MMP
Kim, Seonghan,Cho, Seo-Hyun,Kim, Ka Young,Shin, Ki Young,Kim, Hye-Sun,Park, Cheol-Hyoung,Chang, Keun-A,Lee, Sang Hyung,Cho, Daeho,Suh, Yoo-Hun Blackwell Publishing Ltd 2009 Journal of Neurochemistry Vol.109 No.5
<P>Abstract</P><P>Although there is known to be a marked concentration of reactive microglia in the substantia nigra pars compacta (SNpc) of patients with Parkinson’s disease (PD), a disorder in which &agr;-synuclein plays a key pathogenic role, the specific roles of &agr;-synuclein and microglia remains poorly understood. In this study, we investigated the effects of &agr;-synuclein and the mechanisms of invasive microglial migration into the SNpc. We show that &agr;-synuclein up-regulates the expressions of the cell adhesion molecule CD44 and the cell surface protease membrane-type 1 matrix metalloproteinase through the extracellular regulated kinases 1/2 pathway. These concurrent inductions increased the generation of soluble CD44 to liberate microglia from the surrounding extracellular matrix for migration. The effects of &agr;-synuclein were identical in BV-2 murine microglial cells subjected to cDNA transfection and extracellular treatment. These inductions in primary microglial cultures of C57Bl/6 mice were identical to those in BV-2 cells. &agr;-Synuclein-induced microglial migration into the SNpc was confirmed <I>in vivo</I> using a 6-hydroxydopamine mouse model of PD. Our data demonstrate a correlation between &agr;-synuclein-induced phenotypic changes and microglial migration. With the recruitment of the microglial population into the SNpc during dopaminergic neurodegeneration, &agr;-synuclein may play a role in accelerating the pathogenesis of PD.</P>
Development of the Beamline Front End at the Pohang Light Source(PLS)-II
Myeongjin Kim,Chasun Lee,Seonghan Kim,Hiseob Kim,Chungil Ryu,Induk Seo,Seungnam Kim 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.8
The third-generation synchrotron radiation accelerator has been providing beams to users since 1995 and have been made to upgrade the performances of the accelerators and the experimental devices. For that reason, the PLS-II was started in 2009 as a three-year project. An important feature is the extension of the ID (insertion device) beamline and the ecient placement of the storage ring magnet structure. The overall structure of the beamline as well as the length and structure of the front end had to be changed to expand the ID beamline. Also, the safety of many vacuum parts that would be exposed to the large heat due to the increase in the beam energy was rstly considered in the design, and an analysis was performed in parallel. This paper describes the overall structure of the front end, including the design and analysis of the vacuum components in the Pohang Light Source(PLS)-II beamline.
Choi, Yoori,Kim, Hye-Sun,Shin, Ki Young,Kim, Eun-Mee,Kim, Minji,Kim, Hyun-Soo,Park, Cheol Hyoung,Jeong, Yun Ha,Yoo, Jongman,Lee, Jean-Pyo,Chang, Keun-A,Kim, Seonghan,Suh, Yoo-Hun American College of Neuropsychopharmacology 2007 Neuropsychopharmacology Vol.32 No.11
Minocycline is a semi-synthetic tetracycline antibiotic that effectively crosses the blood–brain barrier. Minocycline has been reported to have significant neuroprotective effects in models of cerebral ischemia, traumatic brain injury, amyotrophic lateral sclerosis, and Huntington's and Parkinson's diseases. In this study, we demonstrate that minocycline has neuroprotective effects in in vitro and in vivo Alzheimer's disease models. Minocycline was found to attenuate the increases in the phosphorylation of double-stranded RNA-dependent serine/threonine protein kinase, eukaryotic translation initiation factor-2 α and caspase 12 activation induced by amyloid β peptide<SUB>1–42</SUB> treatment in NGF-differentiated PC 12 cells. In addition, increases in the phosphorylation of eukaryotic translation initiation factor-2 α were attenuated by administration of minocycline in Tg2576 mice, which harbor mutated human APP695 gene including the Swedish double mutation and amyloid β peptide<SUB>1–42</SUB>-infused rats. We found that minocycline administration attenuated deficits in learning and memory in amyloid β peptide<SUB>1–42</SUB>-infused rats. Increased phosphorylated state of eukaryotic translation initiation factor-2 α is observed in Alzheimer's disease patients' brains and may result in impairment of cognitive functions in Alzheimer's disease patients by decreasing the efficacy of de novo protein synthesis required for synaptic plasticity. On the basis of these results, minocycline may prove to be a good candidate as an effective therapeutic agent for Alzheimer's disease.Neuropsychopharmacology (2007) 32, 2393–2404; doi:10.1038/sj.npp.1301377; published online 4 April 2007
Seonghan Kim,장락우 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.7
Full atomistic molecular dynamics simulations have been performed for model mixture bilayer membrane systems consisting of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) phospholipids to understand the effects of two essential parameters such as lipid composition and temperature on the structural, dynamical, and phase behavior of mixture membrane systems. Although pure DSPC membranes are in the gel-like (Lβ ′ or P β ′) phase at 323 K, raising the temperature by only 10 K or replacing 20% of DSPC lipids by DOPC lipids can change the gel-like phase into the completely liquid-crystalline phase (L α ). This phase change is accompanied by dramatic change in both structural properties such as area per lipid, membrane thickness, deuterium order parameter, and tail angle distribution, and dynamics properties such as mobility map. We also observe that the full width at half-maximum (FWHM) data of tail angle distribution as well as area per lipid (or membrane thickness) can be used as order parameters for the membrane phase transition.
실시간 공중보건 감시와 식품매개질환 집단발생 대처를 위한 국가 전염병 조기경보 시스템 구축 - PulseNet Korea
김석호,이상원,김성한,김준영,이혜영,강연호,박미선,이복권 대한감염학회 2006 감염과 화학요법 Vol.38 No.6
Foodborne illnesses and big outbreaks have been increased because of the widespread of lunch distribution at school, mass production of food products, and international food trades. It is important to find the origin of contamination by various pathogens in an early stage of the outbreaks for the disease control and prevention. For the purpose of construction of the early warning system, Korea National Institute of Health (KNIH) inaugurated PulseNet Korea in 2005. The organization of PulseNet Korea consists of KNIH as a center and the participating laboratories including Korea Food & Drug Administration (KFDA), National Veterinary Research & Quarantine Station (NVRQS), and regional Institutes of Health & Environment. PulseNet Korea has focused on training researchers from participating laboratories as well as playing an important role in PulseNet International. In this review, PulseNet Korea construction is introduced as a national early warning system for timely surveillance of foodborne diseases.