염소를 포함한 액체에서의 금박막 패터닝과 표면 분석

성덕경,최정옥,안일신,김옥경 한양대학교 이학기술연구소 2004 이학기술연구지 Vol.7 No.

금박막은 높은 전도성의 화학적인 안정성을 가지고 있으므로 GaAs를 기판으로 한 bevice에서 널리 쓰이고 있으며 SAM(Self-Assembled Monolayer), Nanolithography 등에서도 아주 주요하게 사용되고 있다. 본 연구에서는 염소가 포함된 액체에 금박막이 접해있고 UV를 조사하여 식각이 되는 것을 발견하고 연구하였다. 노광량을 조절하여 금박막의 기각률을 조절할수 있으며, 또한 가모강제 공정을 거치지 않고 금박막에 직접 조사하게 되므로 간단하게 패터닝을 할수 있다. AFM(atomic force microscope), optical microscope, ellipsometry 를 이용하여 식각률 측정 및 표면 변화를 보았다. Various metals or their alloys are used in the semiconductor metallization process or in micro-or nano-structure devices. We report a novel etching technique of gold film based on new phenomenon that gold is soluble in chlorine containing solutions or solvents under UV irradiation. Mask patterns can be transferred to gold surface directly without resorting to complicate photoresist process. And, etch rate could be controlled from sub nanometer to few tens of nanometers per minute by adjusting exposure parameters. Moreover, non-toxic liquid such as NaCl solution can be used for the process. AFM(atomic force microscope), optical microscope, ellipsometry were used to characterize the etch rate and the surface morphology.

전압형 인버터에 의한 直流 스폿鎔接器의 定電力制御에 關한 硏究

김재문,김학성,박성수,원충연,김덕헌 成均館大學校 科學技術硏究所 1996 論文集 Vol.47 No.1

In recent years, there is increasing interests in spot welder with industrial growth of automobile. It makes autowelding and short-time welding possible. Conventional spot welders using AC source bring about the depreciation of welding products, because constant current control makes its workpiece spattered during welding process. In this paper, the constant power control strategy using PI controller is proposed to improve the quality of the welding products. PI controller is implemented by l6bit one chip 80C196KB microcontroller. Simulation and experimental results show that DC spot welder with single phase PWM inverter is effectively controlled by the constant power control method in very short-time.

소화관내 이물에 대한 고찰

윤한덕,류진호,박주경,허탁,조석주,김성근,민용일 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

We analyzed records at Chonnam university hospital from Sep. 1. 1993 to Aug. 31. 1996 to assess clinical significance of the foreign body in the gastrointestinal tract. There were 133 episodes consisting of 79 pediatric and 54 adult patients as possible to review. In the pediatric patients, the coin was the most common cause of foreign body ingestion, while adult patients were usually caused by bones and meat. We removed foreign bodies in the oral cavity and oropharynx in 12 cases using direct laryngopharyngoscpy and forcep. Endoscopy was performed successfully in 47 of 51 patients in which was attempted; there were two complications composed of an aspiration pneumonia and a mediastinitis due to esophageal perforation. There were spontaneous passage of foreign bodies in 48 cases. We observed complications in 6 cases in which 4 cases needed operations due to complications. Foreign bodies which pass into the stomach can be observed for development of complications, as 94% of foreign bodies which reach the stomach spontaneously pass. Only 4 patients required interventions, which was done without mortality.

백서의 치아이동 시 피질골 천공이 치주조직의 MMP-1,-8, -13 mRNA의 발현에 미치는 영향

곽춘,김성식,박수병,손우성,김용덕,전은숙,박미화 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

Objective: The aim of this study was to determine whether cortical punching stimulates the expression of matrix metalloproteinase-1, -8, and -13 in orthodontic tooth movement in rats. Methods: A total of 32 male sprague-dawley rats at 15 weeks old were divided into two groups of 16 rats each, to form the tooth movement with cortical punching (TMC) group and tooth movement only (TM) group. A total of 20 gm of orthodontic force was applied to rat incisors to cause experimental tooth movement. Cortical punching was performed on the palatal side near the central incisor with a 1.0 mm width microscrew in the TMC group. The duration of tooth movement was 1, 4, 7, and 14 days. Results: Measurements of the mRNA expression were selected as the means to determine the identification of expression of MMP-1, -8, and -13. In the TMC group, the expression of collagen type I was greater than that of the TM group from day 4 to day 14. Expression of TIMP-1 in the TM group was greater than that of the TMC group in the pressure side of PDL and alveolar bone cell at day 4. In the TMC group, TIMP-1 was expressed at the osteoclast, but not at the tooth surface of the TM group at day 14. Maximum induction of the mRNA of MMP-1 was observed on day 4 in the TMC group, but it was observed on day 7 in the TM group. MMP-8 mRNA of the TMC group was twice greater than that of the TM group at 4 days. In the TMC group, maximum induction of MMP-13 mRNA was observed on day 1. Conclusions: These findings suggested that cortical punching can stimulate remodeling of PDL and alveolar bone connective tissues during experimental orthodontic tooth movement in rats. 본 연구는 피질골 천공을 동반한 교정적인 치아이동이 통상의 교정적인 치아이동과 조직 재형성의 정도에 차이가 존재하는지 알아보기 위하여 시행하였다. 실험동물은 15주령 군 300 - 350 g의 자성 백서(Sprague-Dawley Rat)를 사용하였으며 피질골 천공을 동반한 치아이동 실험군(TMC group, n = 16)과 단순 치아이동 대조군(TM group, n =16)으로 나누었다. 모든 실험동물에 20 gm 이하의 힘으로 전치부를 이개시키는 치아 이동을 시행하였으며, 실험군은 상악전치부의 직후방 구개측에서 치근을 손상시키지 않도록 직경 1.0 mm의 microscrew를 사용하여 피질골 천공을하였다. 모든 실험동물은 1, 4, 7, 14일에 희생시켜서 matrix metalloproteinase (MMP)-1, -8, -13 효소의 RT-PCR과 I형 교원질(Collagen type I)과 Tissue inhibitor of metalloproteinase-1 (TIMP-1)의 면역조직화학염색(immunohistochemistry) 을 시행하였다. MMP-1은 실험군에서 4일째에 가장 활발한 발현을 보이다가 7일째와 14일째에 조금씩 감소하는 경향을 보인 반면에, 대조군에서는 실험 7일째에 가장 높은 발현을 보이다가 14일째 들어서 감소하는 양상을 보였다. MMP-8은 실험 1일째부터 실험군이 대조군에 비해서 더 많은 발현을 보였으며, 특히 4일째의 실험군에서는 대조군보다 두 배 이상 발현되었다. MMP-13은 실험 1일째의 실험군에서 가장 높은 발현도를 보였으며, 대조군에서는 오히려 미처치 대조군에 비해 더 낮게 발현되었다. I형 교원질의 면역조직화학염색 결과는 실험 4일째부터 압박측 에서는 실험군에서 대조군보다 더 많은 교원질의 소실을 보였고, 인장측에서는 대조군이 실험군보다 더 많은 교원질의 염색 소견을 보였다. TIMP-1은 실험 4일째에는 압박측에서 대조군이 실험군보다 치주인대와 치조골의 골세포에서 좀 더 균일한 염색 소견을 보였다. 이상의 실험 결과에서 피질골 천공은 백서의 실험적 치아이동에 있어 치주인대와 치조골 결합 조직의 재형성 속도를 증가시키는 것으로 판단된다.

경추손상이 의심되는 환자에서 경추전방 연부조직 폭 측정의 의의

박주경,김성근,윤한덕,허탁,조석주,민용일 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Prevertebral soft tissue swelling of cervical spine lateral radiogram is well known as an indirect evidence of occult cervical spine injury. But the clinical and statistical value of it has been of debate. We tried to analyse the value of cervical prevertebral soft tissue width as a screening test for cervical spine injury by comparison of two categorized study groups; the control group, traumatized patients without cervical spine injury and the injury and the injury group, traumatized patients with bony cervical injury. The injury group consisted of patients admitted between September 1993 and December 1994 with an ED diagnosis of bony cervical injury. The control group consisted of patients admitted between January 1994 and June 1994 who received cervical spine lateral radiograph because of suspicion of cervical spine injury or as a routine check. In both group, we sampled the patients who received cervical spine lateral rediograph within 24 hours after injury and excluded the patients less than 15 years old and more than 65 years old, In the control group, we confirmed the patients had no problem in cervical spine of follow-up. 101 control patients and 68 injury patients were identified. The injury group was further divided into two subgroups: those with injuries at C1-C3 to the upper injury subgroup and those with injuries at C4-C7 to the lower injury sub group. The prevertebral soft tissue widths of injury group were larger than of control group at C2(mean 6.1mm versus 4.5mm) and also at C6(mean 13.4mm versus 11.2mm). Especially upper injury subgroup had quite large value of C2 prevertebral soft tissue width(mean 14.1mm). Nevertheless, both in C2 and C6, there is no cutoff value with acceptable sensitivity and specificity as a screening test. But the 7mm at C2 and 14mm at C6 had high specificity(90%) and were thought to be a good radiologic indicators of further evaluation.

골격성 III급 부정교합자의 수직적 안면골격형태와 교합평면과의 관계

정우준,김성식,손우성,김용덕 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.4

백서의 치아이동 시 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 미치는 영향

박우경,김성식,박수병,손우성,김용덕,전은숙,박미화 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

치아이동 시 피질골 천공이 치조골 재형성에 미치는 영향을 알아보기 위해서 생후 15주된 자성백서를 사용하여 피질골 천공 후 치아이동을 실시한 실험군(Tooth movement with cortical punching: TMC group, n = 16)과 교정적 치아이동만 실시한 대조군(Tooth movement only group: TM group, n = 16)의 치아주위조직을 면역조직화학염색을 통하여 관찰하였다. 실험군과 대조군의 실험동물에 20 gm의 힘으로 상악 전치부 사이를 이개시키는 치아이동을 시행하였으며 실험군에서는 상악 전치부 구개부위에 피질골 천공을 실시하였다. 치아이동 후 1, 4, 7, 14일째에 실험군과 대조군의 실험동물을 희생시켰다. 면역조직화학염색법으로 OPG, RANK, RANKL의 발현을 비교한 결과, OPG의 발현은 양 군 모두에서 미처치 대조군에 비하여 감소되었으나, 실험군에서의 발현이 대조군보다 컸으며, RANK, RANKL은 피질골 천공을 시행한 경우에 더 강한 발현을 보이는 것이 관찰되었다. 따라서 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 영향을 미치며 치조골의 재형성을 향상시키는 것을 알 수 있었다. Objective: The purpose of this study was to investigate whether cortical punching could stimulate the expression of OPG, RANK, and RANKL during tooth movement by immunohistochemistry. Methods: 34 sprague-dawley rats (15 weeks old) were allocated into 3 groups: TMC group (experimental group; Tooth Movement with Corticotomy, n = 16), TM group (control group; Tooth Movement only group, n = 16), and non-treatment group (n = 2). 20 gm of orthodontic force was applied to rat incisors by inserting elastic bands. The duration of force application was 1, 4, 7 and 14 days. A microscrew (diameter 1.2 mm) was used for cortical punching of the palatal side of the upper incisors in the TMC group. Results: Distributions of OPG, RANK, and RANKL were evaluated by immunohistochemistry. OPG, RANK and RANKL were observed on experimental and control groups. On the compression side, the degree of the expression of OPG decreased in both groups. The expression of RANK was most prominent in the experimental group of day 4. The expression of RANKL was most intensive and extensive in the experimental group of day 7. However, the expression of OPG was decreased in the experimental and control groups compared to the non treatment group. The expression of OPG, RANK and RANKL after force application were decreased at day 14. Conclusions: These findings suggested that cortical punching might stimulate remodeling of alveolar bone during a 2 week period of tooth movement without any pathologic change.

어린이에서 수술 중 엎드린 자세와 누운 자세에서의 심박수변이도의 비교

김혜경,김종성,김성덕,김희수,염명걸,백진우 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.4

Comparison of Heart Rate Variability between the Supine and Prone Plsition during General Anesthesia in Children Hee-Soo Kim, M.D., Jin-Woo Baik, M.D., Chong Sung Kim, M.D., Seong Deok Kim, M.D. Myung0Kul Yum, M.D.^*, and Hae Kyung Kim, M.D.^?? Department of Anesthesiology, College of Medicine, Seoul National University ^*Department of Pediatrics, College of Medicine, Hanyang University ^??Department of Anesthesiology, College of Medicine, Inha University, Incheon, Korea Background: It is well known that the prone position affects hemodynamics. In this study, we investigate whether the prone position will affect the heart rate variability compard with the supine position. Methods: Sixty five pediatric patients were assigned to supine group (n = 36) and prone group (n = 29) according to position during surgery. The patients were indeced with thiopental and vecuronium and maintained with O_2-enflurane-N_2O. ECG data was collected for 15 minutes after saturation at the end tidal concentration of the inhalent agent. From the ECG data, power spectral analysis was performed and approximate entropy was calculated. Results: IN the prone group, low frequency power and ratio of low to high frequency power were lower and approximate entropy was higher than those in the supine group. Mean heart rate and blood pressure were no different between the two groups. Conclusions: IN the prone position, sympathetic autonomic activity and balance of the autonomic nervous system decreased. However the complexity of regulation of heart rate variability was maintained in the prone position. (Korean J Anesthesiol 2001; 41: 455~460)

Antibacterial effects of two cecropin type peptides isolated from the silkworm against Salmonella species

( Seong Ryul Kim ),( Jong Woo Park ),( Seong-wan Kim ),( Su Bae Kim ),( You-young Jo ),( Kee Young Kim ),( Kwang-ho Choi ),( Sang Deok Ji ),( Jong Gil Kim ),( Haeyong Kweon ) 한국잠사학회 2018 International Journal of Industrial Entomology Vol.37 No.2

In insect defense system, antimicrobial peptides (AMPs) are one of important biological molecules to survive in a variety of environments. Insect can synthesize AMPs to protect against invading pathogens in humoral immune response. Taking more advantage of biological antimicrobial molecules, we report antibacterial activity of two cecropin type peptides, cecropin and moricin, isolated from the silkworm against four salmonella species. In this work, we purified antimicrobial candidate peptides (AMCP) from the extracts of immune challenged silkworm larval hemolymph by two-step chromatographic purification procedure, cation exchange and gel permeation chromatography. The molecular weights of purified peptides were estimated to be about 4 ~ 5 kDa by Tricin SDS-PAGE analysis, and identified as silkworm cecropin and moricin by NCBI BLAST homology search with their N-terminal amino acid sequences. As antibacterial activity assay, the purified peptides showed stronger antibacterial activity against Salmonella pathogens with an MIC value of 1 ~ 4 μg/mL. Therefore two cecropin type peptides purified from the silkworm will be valuable potential materials for development of new natural antibiotics.

Antibacterial effects of two cecropin type peptides isolated from the silkworm against Salmonella species

Kim, Seong Ryul,Park, Jong Woo,Kim, Seong-Wan,Kim, Su Bae,Jo, You-Young,Kim, Kee Young,Choi, Kwang-Ho,Ji, Sang Deok,Kim, Jong gil,Kweon, HaeYong Korean Society of Sericultural Science 2018 International Journal of Industrial Entomology Vol.37 No.2

In insect defense system, antimicrobial peptides (AMPs) are one of important biological molecules to survive in a variety of environments. Insect can synthesize AMPs to protect against invading pathogens in humoral immune response. Taking more advantage of biological antimicrobial molecules, we report antibacterial activity of two cecropin type peptides, cecropin and moricin, isolated from the silkworm against four salmonella species. In this work, we purified antimicrobial candidate peptides (AMCP) from the extracts of immune challenged silkworm larval hemolymph by two-step chromatographic purification procedure, cation exchange and gel permeation chromatography. The molecular weights of purified peptides were estimated to be about 4 ~ 5 kDa by Tricin SDS-PAGE analysis, and identified as silkworm cecropin and moricin by NCBI BLAST homology search with their N-terminal amino acid sequences. As antibacterial activity assay, the purified peptides showed stronger antibacterial activity against Salmonella pathogens with an MIC value of $1{\sim}4{\mu}g/mL$. Therefore two cecropin type peptides purified from the silkworm will be valuable potential materials for development of new natural antibiotics.