Combined Gene Therapy with Hypoxia-Inducible Factor-1α and Heme Oxygenase-1 for Therapeutic Angiogenesis

Bhang, Suk Ho,Kim, Ju Hee,Yang, Hee Seok,La, Wan-Geun,Lee, Tae-Jin,Kim, Ga Hee,Kim, Hyun Ah,Lee, Minhyung,Kim, Byung-Soo Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.7

<P>Transfection with either hypoxia-inducible factor-1α (HIF-1α) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1α gene occurs rapidly, the expressed HIF-1α protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1α and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1α or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1α and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1α gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1α and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.</P>

복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

인천국제공항 탑승동에 적용 가능한 적정 공조시스템에 관한 연구

석호태,정만석,김지현,여명석,김광우 대한건축학회 2005 대한건축학회논문집 Vol.21 No.1

Generally, indoor thermal environment control consumes much energy in the building Appropriate performance evaluation of the building and HVAC facilities are achieved by an energy simulation The result Which does the energy simulation of property HVAC system in airport Primary, there seems to be reasonable method which save window conduction loads and solar radiation loads on the element for an energy saving Secondly, According to the existence of contact outside in building Loads, the Important factor of influence is divided into solar radiation, window conduction loads and interior loads(lighting, equivalent, occupancy etc) The last, the result which does the energy simulation of each HVAC system, CAV + V AV System is determined profitably in energy performance

건물에너지 성능 진단 및 개보수 기법

석호태 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

In this study, the influence of energy factors on energy consumption in a selected office building is analyzed by sensitivity analysis. Dominant energy factors of each building and system are determined. Then, the energy management schemes for these factors are suggested. If this method is applied to other office buildings, it may be possible to work out a strategy of energy diagnosis and retrofit more efficiently.

冷凍冷藏倉庫 前室部 侵氣防止裝置의 適用에 關한 硏究

석호태,곽현철,송승영,송진규,이영욱 대한건축학회 2003 대한건축학회논문집 Vol.19 No.4

This study aims to evaluate the economic performance of infiltration prevention devices for the refrigerated warehouse. It is showed that the cooling loads of refrigerated warehouses is changed by assembling of infiltration prevention devices in a previous study. Space loads and cooling energy costs according to the types of the infiltration prevention devices are analyzed by the DOE-2.1E simulation. Based on the Life Cycle Costs considered the costs of initial construction, repair·replace, cooling energy and operating energy, the probability of reducing energy consumption and application of infiltration prevent devices in vestibule of refrigerated warehouse is founded.

腦性麻痺의 東·西醫學的 文獻考察

劉昊相,吳旼錫,宋泰元 대전대학교 한의학연구소 2000 한의학연구소 논문집 Vol.9 No.1

In the literature study on the cerebral palsy, the results were as follows : 1.Cerebral palsy is defined as a disorder of movement and posture due to a defect or lesion of the immature brain. For practical purposes it is useful to exclude from cerebral palsy those disorders of posture and movement which are of short duration, due to a progressive disease, due solely to mental deficiency. 2.Cerebral Palsy is classified with quadriplegia, diplegia, hemi plegia, triplegia, and monoplegia or spastic CP, athetoid CP, ataxic CP, and combined classifications. 3.Causes of Cerebral Palsy is any damage with prenatal, natal and postnatal causes. 4.Management consists of helping the child achieve maximum potential in growth and development. This should be started as early as possible with identification of the very young child who may have a developmental disorder. Certain medications, surgery, and braces may be used to improve nerve and muscle coordination and prevent dysfunction. 5.The aim of treatment is to encourage children and adults to learn to be as independent as possible. Some children and adults who have midl cerebral palsy will have no problems in achieving independence 6.Oji(五遲),Oyeon(五軟) and Okyeong(五硬) have the simmiar concepts with the cerebral palsy. 7.Oji(五遲) Oyeon(五軟) and Okyeong(五硬) are caused by seoncheon-pumbu-bujok(先天稟賦不足) and related with gan(肝), bi(脾) and sin(腎). 8.The treatment is achieved by the method of bogansin(補肝腎), ganggeungol(强筋骨) and bojungikki(補中益氣). And jihwanghwan(地黃丸) has been used most frequently.

生物學的 改良劑를 利用한 슬럿지의 脫水

김형석,신남철,이태호,박승조 동아대학교 환경문제연구소 1991 硏究報告 Vol.14 No.2

To conditioning sludge to occure in the course of food processing industry, after culturing Rhoodotorula glutinis, dosing a certain amount of culture solution into the sludge and measured the variation of total suspended solids and then, in the aerobic and anaerobic conditions after manage sludge, examining the result of measured specific resitance, the coefficient of cmopressibility to know the dewateability. Arrangements of this result are as follows : 1) As the result of total suspended solids concentration by change of aeration time and the amount of Rhodotorula glutinis dose, optimal dose was Rhodotorula glutinis 10㎖ by 20㎖ sludge. The very suitable aeration time was 4-5days. 2) After dosing biological conditioner(Rhodotorula glutinis) 60㎖ by 11 sludge in aerobic condition, aeration for five days. The results of specific resistance, the coefficient of compressibility were 22.71×10^(5)m/㎏ 1.6, respectively. 3) In the cases of using Rhodotoruls glutinis for biological conditioner, using polymer for chemical conditioner. This results are as follows : Biological conditioner ; the moisture content of sludge cake 77.7% Chemical conditioner ; the moisture content of sludge cake 70.3% But, I author propose that biological conditioner is more useful than chemicals in the total operating and maintenance cost.

한국산 닥나무류의 해부학적, 화학적 성질 및 펄프화 특성

최태호,조남석,김병로 충북대학교 농업과학기술연구소 1991 農業科學硏究 Vol.9 No.2

This study was carried out to investigate the papermaking characteristics for New Hanji (Korean Paper) products from paper mulberries. So far the bast fibers of paper mulberry species have been used as raw materials for Hanji. But, in this study, bast fibers, woody parts and whole woods are attempting for being utilize as new raw materials for special Hanji. Anatomical and chemical properties of 3 paper mulberry species and their pulping characteristics were discussed.

이온교환된 ZSM-23 제올라이트 내에서 톨루엔 메탈화반응의 형상선택성

박동호,김태석 인제대학교 1992 仁濟論叢 Vol.8 No.2

이온 교환된 ZSM-23 제올라이트를 이용하여 톨루엔의 메틸화 반응에서의 반응 활성과 파라-선택성(p-선택성)을 실험했다. ZSM-5 제을라이트를 ZSM-23 제올라이트와 비교 실험하였다. 먼저 실험에 사용된 제을라이트 촉배로써 ZSM-23은 1.6Na2O-A1203-59. OSi02-17.9pyrrolidine-584H20, ZSM-5는 2.9Na20-A1203-1. 1H2S04-49.9Si02-4.6TPABr-1790H2O의 초기 조성비로 합성하였다. XRD와 SEM을 이용하여 합성된 ZSM-23과 ZSM-5 제올라이트의 결정 성 과 골격 구조 및 입 자 모양을 확인할 수 있었다. 합성 된 ZSM-23, ZSM-5 제올라이트에 알칼리금속을 수용액상에서 이온 교환시켜 교환된 이온종과 교환된 양에 따른 반응 활성과 선택성의 변화를 조사하였다. 즉, 1M의 NH4NO3, NaCl, CsCI의 부피를 달리하여 이온 교환량을 조절하면서 H-, Na- , Cs-제올라이트를 얻었다. 톨루엔 메틸화 반응이 일어날때 ZSM-23의 경우 HZSH-23이 반응 활성은 가장 높았고, 교환된 Na+량이 증가할수록 반응활성은 낮아지고 p-선택성은 증가되었다. 2SM-23의 경우 아주 적은 양의 Na+이온이 교환되었을 때에도 큰 Na+이온 반경에 의한 m-크실렌과 o-크실렌의 확산 지연 효과 때문에p-선택성을 나타내었다. ZSM-5의 경우 HZSM-5일 때 반응 활성은 HZSM-23 제올라이트보다 높았다. 그러나 NaZSM-23과는 달리 NaZSM-5에서 p-선택성의 증가는 나타나지 않았다. 이러한 결과는 ZSM-5의 세공 크기가 ZSM-23보다 약간 크기 때문이다. ZSH-23에 이온 교환된 경우 세공 구조의 특성에 의해 같은 조건으로 이온 교환시킨 ZSM-5보다 p-선택성이 높음을 확인할 수 있었다.

原木 표고 栽培 收益性 調査

崔泰鎬,閔斗植,趙南奭,趙修玉 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.11 No.1

For the investigation of the economics of mushroom(Lentinus erodes) cultivation in the oak bed- log, the items of production costs were examined. The results are as follows ; 1. Among the items of cultivation costs in the first year, the material bed-log cost(62.4% ) was the highest, then the labor cost was. 2. The labor costs for management(27.6-28.1%), bed-log handling(26.8-27.9% ) and mushroom harvesting(26.2-27.9%) were higher than the others from the 2nd year to the sixth year. 3. The gross income and the cultivation cost per one bed- log were 15,672 won and 3,170 won for six years. 4. For six years, the total net income was 12,500 won and the annual income was 2,080 won per one bed- log.