[BOOK REVIEW] Niyoga : Alternative mechanism to lineage perpetuation in early India (A socio-historical enquiry)

Saswati Bhattacharya 이화여자대학교 아시아여성학센터 2018 Asian Journal of Women's Studies(AJWS) Vol.24 No.4

Multiple biological properties of macelignan and its pharmacological implications

Saswati Paul,황재관,김한영,Won Kyung Jeon,정지혜,한정수 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.3

Macelignan found in the nutmeg mace ofMyristica fragrans obtains increasing attention as a newavenue in treating various diseases. Macelignan has beenshown to possess a spectrum of pharmacological activities,including anti-bacterial, anti-inflammatory, anti-cancer,anti-diabetes, and hepatoprotective activities; recently, it hasalso been shown to have neuroprotective activities. Thisreview summarizes the current research on the biologicaleffects of macelignan derived from M. fragrans, withemphasis on the importance in understanding and treatingcomplex diseases such as cancer and Alzheimer’s disease.

Niyoga: Alternative mechanism to lineage perpetuation in early India (A socio-historical enquiry)

Saswati BHATTACHARYA 이화여자대학교 아시아여성학센터 2018 Asian Journal of Women's Studies(AJWS) Vol.24 No.4

Impact of miRNA deregulation on mRNA expression profiles in response to environmental toxicant, nonylphenol

Saswati Paul,김승준,박혜원,이승용,안유리,오문주,정진욱,류재천,황승용 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.3

MicroRNA (miRNA) are approximately 22nt RNA molecules with the ability to regulate gene expression by interacting with its target mRNA. Recent studies demonstrated that miRNA are responsible for determining cell fate and also plays an important role in cellular response to xenobiotics stress and other toxicological phenomenon. Also a number of reports have strengthened the correlation between altered miRNA expression and various cancers. In this study miRNA and mRNA expression profiling was carried out in in-vitro differentiating MCF-7 and HepG2 cell line to understand the effect of nonylphenol (NP), an industrially synthesized environmental toxicant. Analyzing the mRNA- miRNA interaction, we observed correlation between deregulated miRNAs and its predictive differentially expressed target mRNA. We also observed differential expression of two widely studied miRNAs,miR-21 and miR-34 in these cell lines. CTCF is found to be the predictive target of expressed miR-21 in MCF-7. In HepG2 cell line expressed MDM2 is found to be a predictive target of miR-34b. These results support the possible role of miRNA interaction in the expression of its target genes and also ability of environmental toxicant to deregulate miRNA expression.

Silver‑incorporated hydroxyapatite–albumin microspheres with bactericidal effects

Saswati Mishra,Tapash R. Rautray 한국세라믹학회 2020 한국세라믹학회지 Vol.57 No.2

Bacterial adhesion on HA-coated implant is a serious problem that often leads to bone infection such as osteomyelitis on implant sites. Gram-positive bacteria such as Staphylococcus aureus are responsible for such infection-forming biofilm on implant surfaces. Therefore, inclusion of antimicrobial agents especially inorganic ions such as silver has gained vogue due to their strong antibacterial effects. This study reports the fabrication of porous Ag–albumin–HA microspheres by waterin- oil emulsion technique. Varied proportions of porogen with HA ensured enhanced pores in the fabricated microsphere. Further, SEM analysis revealed the presence of microspheres in the range ~ 50–900 μm with interconnected pores ranging between ~ 10 and 50 μm. Antibacterial efficacy of the Ag–HA hybrid disrupting the biofilm was observed. The as-formed microspheres having interconnected pore channels are envisaged to show vascularization as well as fulfilling goals of ideal bone filler ensuring biocompatibility and reducing long-term cytotoxicity at implant site.

Alteration in miRNA Expression Profiling with Response to Nonylphenol in Human Cell Lines

Paul, Saswati,Kim, Seung-Jun,Park, Hye-Won,Lee, Seung-Yong,An, Yu-Ri,Oh, Moon-Ju,Jung, Jin-Wook,Hwang, Seung-Yong The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.1

Exposures to environmental chemicals that mimic endogenous hormones are proposed for a number of adverse health effects, including infertility, abnormal prenatal and childhood development and above all cancers. In addition, recently miRNA (micro RNA) has been recognized to play an important role in various diseases and in cellular and molecular responses to toxicants. In this study, endocrine disrupting environmental toxicant, nonylphenol (NP) was treated to MCF-7 (Human breast cancer cell) and HepG2 (Human hepatocellular liver carcinoma) cell line at 3 hrs and 48 hrs time point and miRNA analysis using $mirVana^{TM}$ miRNA bioarray was performed and compared with total mRNA microarray data for the same cell line and treatment. Robust data quality was achieved through the use of dye-swap. Analysis of microarray data identifies a total of 20 and 11 miRNA expressions at 3 hrs and 48 hrs exposure to NP in MCF-7 cell line and a total of 14 and 47 miRNA expression at 3 hrs and 48 hrs exposure respectively to NP in HepG2 cell line. Expression profiling of the selected miRNA (let-7c, miR-16, miR-195, miR-200b, miR200c, miR-205, and miR-589) reveals changes in the expression of target genes related to metabolism, immune response, apoptosis, and cell differentiation. The present study can be informative and helpful to understand the role of miRNA in molecular mechanism of chemical toxicity and their influence on hormone dependent disease. Also this study may prove to be a valuable tool for screening potential estrogen mimicking pollutants in the environment.

Genome-wide TIFY family in Arachis hypogaea in the perspective of legume JAZs

Sen Saswati 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.4

Plant-specifc TIFY transcription factor family is characterized by a highly conserved TIFY domain. Four sub-families ZML, PPD, TIFY, and JAZ (Jasmonate ZIM domain), participate in a wide diversity of developmental and stress-responsive processes. Here, 29 Arachis hypogaea (peanut) TIFY family genes are identifed and characterized by conserved domain distribution, exon–intron pattern, and phylogenetic clustering. 6 ZMLs, 2 TIFYs, 1 PPD, and 20 JAZ sub-family genes are confrmed in AhTIFY family. Since allotetraploid peanut contains two progenitor sub-genomes, 14 homeologous gene pairs are obtained, also showing positional or functional homeology, based on comparative chromosomal localization and comparative tissue expression, respectively. Transcriptomic studies of Peanutbase show tissue-specifc expression, drought, and nematode-responsiveness of AhJAZs. It reveals contribution of JA-signaling pathway in the development and stress management of a globally important oil-crop peanut, fnally to enhance its agricultural production. In connection to AhJAZs, this study has scrutinized legume JAZs as a whole, to fnd out few interesting features of the legume JAZs, viz., (a) the absence of JAZ5, 10, 11 and (b) the presence of Jas intron in the JAZ1s, subjected to alternative splicing, contrasted with Arabidopsis. Most intriguingly, in 13 legume JAZ12s, predicted peptides from the in-frame coding sequence containing three-nucleotide periodicity and termination codon within the Jas intron/3′-intron show sequence similarity to rhizobial ABC transportertype proteins. Predicted peptides appear to be encoded by these intronic sORFs to modulate rhizobial transporter proteins and thereby to infuence the symbiotic relation of the legume crops through a unique way of JAZ utilization in the legumes.

Interaction of basal forebrain cholinergic neurons with the glucocorticoid system in stress regulation and cognitive impairment

Paul, Saswati,Jeon, Won Kyung,Bizon, Jennifer L.,Han, Jung-Soo Frontiers Media S.A. 2015 FRONTIERS IN AGING NEUROSCIENCE Vol.7 No.-

<P>A substantial number of studies on basal forebrain (BF) cholinergic neurons (BFCN) have provided compelling evidence for their role in the etiology of stress, cognitive aging, Alzheimer’s disease (AD), and other neurodegenerative diseases. BFCN project to a broad range of cortical sites and limbic structures, including the hippocampus, and are involved in stress and cognition. In particular, the hippocampus, the primary target tissue of the glucocorticoid stress hormones, is associated with cognitive function in tandem with hypothalamic-pituitary-adrenal (HPA) axis modulation. The present review summarizes glucocorticoid and HPA axis research to date in an effort to establish the manner in which stress affects the release of acetylcholine (ACh), glucocorticoids, and their receptor in the context of cognitive processes. We attempt to provide the molecular interactive link between the glucocorticoids and cholinergic system that contributes to BFCN degeneration in stress-induced acceleration of cognitive decline in aging and AD. We also discuss the importance of animal models in facilitating such studies for pharmacological use, to which could help decipher disease states and propose leads for pharmacological intervention.</P>

Comparative Analysis of Gene Expression Patterns after Exposure to Nonylphenol in Human Cell Lines

오문주,Saswati Paul,김승준,김준섭,윤종필,박혜원,김연정,류재천,이철우,김현미,최경희,김학주,황승용 한국바이오칩학회 2008 BioChip Journal Vol.2 No.4

Nonylphenol (NP), a byproduct of industrial synthesis, is quite similar to estrogen in structure, and is known as an environmental estrogen that induces estrogenic disturbances. It has been utilized in several industries for the manufacture of skin cleaning materials, kitchen detergents, cosmetics, fabric detergents, and ink binder. Due to its characteristic strong estrogenic potency, NP is capable of disrupting the reproductive hormone system. Exposure to NP for a prolonged period increases the chances of developing breast and lung cancers. In this study, we conducted a comparative study of expression profiles between HK (Human Kidney cell), MCF-7 (Human breast cancer cell), and LNCaP (Human prostate cancer cell) cells treated with NP at the value of IC20, using Agilent whole genome microarrays. After comparative analysis, we detected some specific expression patterns in each of the cell lines. However, the expression patterns from the HK-2 and MCF-7 cells are quite similar. Interestingly, estrogen receptor 1 and 2 genes were downregulated only in MCF-7 cells, whereas the androgen receptor (AR) gene evidenced overexpression in all 3 of the cell lines. The PDZK1 gene, which has been identified as an estrogen-responsive gene, has also been shown to be overexpressed in all 3 of the tested cell lines. The majority of differentially expressed genes in NP treatment were shown to be involved in cell proliferation, transcription, and signaling. These results may establish a framework for nderstanding the mechanisms underlying the toxicity of NP.

Application of the Emerging Technologies in Toxicogenomics: An Overview

유소연,Saswati Paul,황승용 한국바이오칩학회 2016 BioChip Journal Vol.10 No.4

Toxicogenomics is a rapidly developing area of research which combines the understanding of molecular and cellular regulation in response to interaction of chemical stressors. Moreover, the emergence of comprehensive research method covering genomic, proteomic, and metabonomics analysis including bioinformatics has further substantiated the field of toxicogenomics. Thus, evaluation of such integrated analysis, conversing from variety of experimental techniques, along with the resulting database can decipher the importantance of such techniques in toxicogenomics. In addition, recent integration of epigenetic study (DNA Methylation, histone modification, small and noncoding RNAs) with toxicogenomics can pave the path to elucidate, highly reliable research results. The objective of the present review is to expand these understandings of toxicogenomics approach and validate the contribution of the developing research methods (genome, proteome, metabolites) to emphasize its importance in future toxicogenomics research.