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Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings
Jacqueline Oliveira dos Santos,Cinthia Aparecida Andrade,Kamila Rezende Dázio de Souza,Meline de Oliveira Santos,Isabel Rodrigues Brandão,Jose Donizeti Alves,Iasminy Silva Santos 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.3
Zinc is an essential micronutrient for the healthy development of plants, since its insufficient and supraoptimal doses can disrupt the metabolism and biomass production. We aimed to investigate the physiological responses of coffee seedlings to Zn deficiency and excess. Six-month-old seedlings were transferred to plastic pots containing a nutrient solution. The treatments were control (0.03 ppm), zinc deficiency (0.00 ppm), and zinc excess (0.12 ppm). The evaluations were performed in leaves and roots at the beginning of the treatments and after 30 and 60 d of treatments. Zn deficiency and excess increased the production of hydrogen peroxide, antioxidant enzymes activity, ascorbate, and lipid peroxidation contents. The imbalance in zinc nutrition reduced total chlorophyll content and increased carotenoids content throughout the experimental period. Lower biomass and proline accumulation were observed only for deficient seedlings at the end of the experiment. The characteristics analyzed showed that zinc deficiency caused greater damage to the Coffea arabica plants of (Catuai cultivar) than zinc excess.
Health-Related Quality of Life, Depression and Anxiety in Hospitalized Patients with Tuberculosis
Santos, Ana Paula Cere dos,Lazzari, Tassia Kirchmann,Silva, Denise Rossato The Korean Academy of Tuberculosis and Respiratory 2017 Tuberculosis and Respiratory Diseases Vol.80 No.1
Background: Much of the attention of tuberculosis (TB) programs is focused on outcomes of microbiological cure and mortality, and health related quality of life (HRQL) is undervalued. Also, TB patients have a significantly higher risk of developing depression and anxiety compared with those in the general population. We intend to evaluate the HRQL and the prevalence of symptoms of depression and anxiety in hospitalized patients with TB. Methods: Cross-sectional study in a tertiary care hospital in Brazil. Adult patients with pulmonary TB that were hospitalized during the study period were identified and invited to participate. HRQL was measured using the Medical Outcomes Study Short Form-36 (SF-36) version 2. Hospital Anxiety and Depression Scale (HADS) was used to record symptoms of anxiety and depression. Results: Eighty-six patients were included in the analysis. The mean age of all patients was $44.6{\pm}15.4$ years, 69.8% were male, and 53.5% were white. Thirty-two patients (37.2%) were human immunodeficiency virus positive. Twenty-seven patients (31.4%) met study criteria for depression (HADS depression score ${\geq}11$) and 33 (38.4%) had anxiety (HADS anxiety score ${\geq}11$). Scores on all domains of SF-36 were significantly lower than the Brazilian norm scores (p<0.001). Conclusion: The present study shows that TB patients may have a poor HRQL. Additionally, we found a possible high prevalence of depression and anxiety in this population. Health care workers should be aware of these psychological disorders to enable a better management of these patients. The treatment of these comorbidities may be associated with better TB outcomes.
( Santos Ebinuma valeria Carvalho ),( Maria Francisca Simas Teixeira ),( Adalberto Pessoa Jr ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
This work aims at investigating the production of yellow, orange, and red natural colorants in a submerged culture of Penicillium purpurogenum DPUA 1275. For this purpose, different experimental conditions evaluating the effect of incubation time, type and size of in℃ulum, and different carbon and nitrogen sources were performed. Furthermore, the growth kinetics were obtained in the conditions of 108 spores/ml and 5 mycelia agar discs during 360 h. These experiments showed that 5 mycelia agar discs and 336 h promoted the highest yellow (3.08 UA400nm), orange (1.44 UA470nm), and red (2.27 UA490nm) colorants production. Moreover, sucrose and yeast extract were the most suitable carbon and nitrogen sources for natural colorants production. Thus, the present study shows a new source of natural colorants, which can be used as an alternative to others available in the market after toxicological studies.
Nitrogen Metabolism in Lactating Goats Fed with Diets Containing Different Protein Sources
Santos, A.B.,Pereira, M.L.A.,Silva, H.G.O.,Pedreira, M.S.,Carvalho, G.G.P.,Ribeiro, L.S.O.,Almeida, P.J.P.,Pereira, T.C.J.,Moreira, J.V. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5
This study aimed to evaluate urea excretion, nitrogen balance and microbial protein synthesis in lactating goats fed with diets containing different protein sources in the concentrate (soybean meal, cottonseed meal, aerial part of cassava hay and leucaena hay). Four Alpine goats whose mean body weight was $42.6{\pm}6.1kg$ at the beginning of the experiment, a mean lactation period of $94.0{\pm}9.0days$ and a production of $1.7{\pm}0.4kg$ of milk were distributed in a $4{\times}4$ Latin square with four periods of 15 days. Diets were formulated to be isonitrogenous, containing 103.0 g/kg of CP, 400 g/kg of Tifton 85 hay and 600 g/kg of concentrate. Diet containing cottonseed meal provided (p<0.05) increased excretion of urea and urea nitrogen in the urine (g/d and mg/kg of BW) when compared with leucaena hay. The diets affected the concentrations of urea nitrogen in plasma (p<0.05) and excretion of urea nitrogen in milk, being that soybean meal and cottonseed meal showed (p<0.05) higher than the average aerial part of the cassava hay. The use of diets with cottonseed meal as protein source in the concentrate in feeding of lactating goats provides greater nitrogen excretion in urine and negative nitrogen balance, while the concentrate with leucaena hay as a source of protein, provides greater ruminal microbial protein synthesis.
Santos, Ilyn L.,Lee, Ju-Young,Youm, Yujin,Lim, Jinkyu Institute of Agricultural Science and Technology 2013 慶北大農學誌 Vol.31 No.2
Nuts are one of the most common sources of allergies in individuals of all ages. In order for a particular protein to render an allergic reaction, it must resist proteolytic digestion by intestinal enzymes. In this study, three well-known allergenic nuts, almonds, cashew nuts, and peanuts, were used as samples, and enzyme digestion with Bacillus protease and porcine pepsin was tested. A proteomic approach using two-dimensional gel electrophoresis and an MS/MS analysis was applied to visualize and identify the proteins that were resistant to enzyme digestion. Among the 150 protein spots tested, 42 proteins were assigned functions. Due to the lack of genomic databases, 41% of the identified proteins were grouped as hypothetical. However, 12% of them were well-known allergens, including AraH. The remainder were grouped as storage, enzymes, and binding proteins.
Elastic Buckling Assessment of Doubly Symmetric I-Beams with Singly Stepped Section at Midspan
Santos. Ralph Raymond V.,Kang Jun Suk,Park Jong Sup 한국방재학회 2017 한국방재학회논문집 Vol.17 No.6
최근 스텝보의 설계기준 활용성을 향상시키기 위한 여러 연구가 진행된바 있다. 그러나 지간 중앙에 스텝단면이 보강된 I형보의 횡-비틀림 좌굴에 대한 연구는 제한적이었다. 본 연구는 지간 중앙에 스텝보가 적용된 I형보에 하중고 효과와 다양한 하중을 고려한 횡-비틀림 탄성좌굴강도를 평가하고자 한다. 범용유한요소해석 프로그램 ABAQUS가 사용되었으며, 쉘 요소인 S4R요소를 모델링에 적용되었고 해석결과는 기존 설계기준 및 기 발표된 제안식과도 비교분석 되었다. 본 논문은 다양한 표와 그림을 활용하여 해석결과를 나타내고 있으며, 해석연구 비교분석을 통하여 새로운 설계식 제안되었다. Over the years, several studies have been made for the improvement of the design criteria of stepped beams. However, studies on lateral-torsional buckling of stepped beams located at the midspan have been very limited. Hence, this study aims to evaluate the elastic lateral-torsional buckling strength of doubly symmetric singly stepped I-beam at midspan considering the load-height effect and various loading conditions. To model and perform the analysis for the I-beams, a universal finite element analysis program, ABAQUS, will be used. In addition, S4R elements will be used to model the simply supported beams where loads are applied at shear center and at top flange, and to check the accuracy of the models guide design specifications are used. The results from the finite element analysis will be shown using tables and illustrative figures. Based from the obtained results, conclusions and new design guidelines are proposed.
Synthesis and Characterization of Dense Ceramic Membranes for Methane Conversion - Part II
Santos A.,Fontes V.A.,Fontes F.A.Oliveira,De Sousa J.F.,De Souza C.P. 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1
The perovskite- type oxide containing transition metals on the B-site show mixed (electronic/ionic) conductivity. These mixed-conductivity oxides are promising materials for oxygen permeating membranes. The main objective of this research work is to synthesize and characterization ceramic powders of the Sr-Co-Fe-O system for methane conversion using membrane reactor. SCFO powders were synthesized from the route was based on the complex method of combination of acid EDTA and citrate and shown be available by control efficient of synthesis to performed , moreover, it presented easy implementation, reproducibility and operation. Powder ceramic was characterized by XRD, microscopic optic, SEM and TG-DTA.
Endophytic Fungi as a Source of Biofuel Precursors
( Santos Fo Florisvaldo C. ),( Taicia Pacheco Fill ),( Joanita Nakamura ),( Marcos Roberto Monteiro ),( Edson Rodrigues Fo ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.7
Endophytic fungi, isolated from a number of different species of tropical plants, were investigated for lipid biodiesel precursor production. The extracts produced from liquid cultures of these fungi were subjected to acidcatalyzed transesterification reactions with methanol producing methyl esters and then analyzed through chromatographic (GC-FID) and spectrometric techniques (MS, NMR ¹H). The European Standard Method, EN 14103, was used for the quantification of methyl esters extracted from the fungi of the species and genera studied. Xylariaceous fungi exhibited the highest concentrations of methyl esters (91%), and hence may be a promising source for biofuel.