Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent

Lee, Seung Hwan,Choi, Jong-il,Han, Mee-Jung,Choi, Jong Hyun,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2005 Biotechnology and bioengineering Vol.90 No.2

<P>We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfully displayed on the surface of Escherichia coli. Localization of the truncated OprF-lipase fusion protein was confirmed by western blot analysis, immunofluorescence microscopy, and whole-cell lipase activity. To examine the enzymatic characteristics of the cell surface displayed lipase, the whole-cell enzyme activity and stability were determined under various conditions. Cell surface displayed lipase showed the highest activity at 37°C and pH 8.0. It retained over 80% of initial activity after incubation for a week in both aqueous solution and organic solvent. When the E. coli cells displaying lipases were used for enantioselective resolution of racemic 1-phenylethanol in hexane, (R)-phenyl ethyl acetate was successfully obtained with the enantiomeric excess of greater than 96% in 36 h of reaction. These results suggest that E. coli cells displaying lipases using OprF as an anchoring motif can be employed for various biotechnological applications both in aqueous and nonaqueous phases. © 2005 Wiley Periodicals, Inc.</P>

Clinical Outcomes of Transcatheter Arterial Embolization after Failed Endoscopic Intervention for Acute Non-Variceal Bleeding Associated with Benign Upper Gastrointestinal Diseases

Sang Yoon Kim,Su Jin Kim,Ayoung Lee,Kichul Yoon,Jun Young Park,Ju Yup Lee,Jae Myung Park 대한상부위장관ㆍ헬리코박터학회 2023 Korean Journal of Helicobacter Upper Gastrointesti Vol.23 No.1

Background/Aims: Transcatheter arterial embolization (TAE) is useful for management of uncontrolled upper gastrointestinal (UGI) bleeding. We investigated clinical outcomes of TAE for non-variceal bleeding from benign UGI diseases uncontrolled with endoscopic intervention. Methods: This retrospective study performed between 2017 and 2021 across four South Korean hospitals. Ninety-two patients (72 men, 20 women) who underwent angiography were included after the failure of endoscopic intervention for benign UGI disease- induced acute non-variceal bleeding. We investigated the factors associated with endoscopic hemostasis failure, the technical success rate of TAE, and post-TAE 30-day rebleeding and mortality rates. Results: The stomach (52/92, 56.5%) and duodenum (40/92, 43.5%) were the most common sites of bleeding. Failure of endoscopic procedures was attributable to peptic ulcer disease (81/92, 88.0%), followed by pseudo-aneurysm (5/92, 5.4%), and angiodysplasia (2/92, 2.2%). Massive bleeding that interfered with optimal visualization of the endoscopic field was the most common indication for TAE both in the stomach (22/52, 42.3%) and duodenum (14/40, 35.0%). Targeted TAE, empirical TAE, and exclusive arteriography were performed in 77 (83.7%), nine (9.8%), and six patients (6.5%), respectively. The technical success rate, the post-TAE 30-day rebleeding rate, and the overall mortality rate were 100%, 22.1%, and 5.8%, respectively. On multivariate analysis, coagulopathy (OR, 5.66; 95% CI, 1.71~18.74; P=0.005) and empirical embolization (OR, 5.71; 95% CI, 1.14~28.65; P=0.034) were independent risk factors for post-TAE 30-day rebleeding episodes. Conclusions: TAE may be useful for acute non-variceal UGI bleeding. Targeted embolization and correction of coagulopathy can improve clinical outcomes.

Introduction to Statistical Inference in Social Network Analysis

Sang Yup Lee(이상엽) 경북대학교 사회과학기초자료연구소 2016 연구방법논총 Vol.1 No.2

이 논문은 사회망 분석 기법 중 통계적 추론 방법에 기반한 지수 랜덤 그래프 모형 (Exponential Random Graph Modeling, ERGM)에 대해 소개한다. 구체적으로, ERGM의 다음과 같은 사항을 다룬다: 1) 사용 목적, 2) 모델 개발 및 설정, 3) 파라미터 추정방법, 4) 결과 해석 방법. 이론적인 설명과 더불어 ERGM이 실제 네트워크 관련 연구에 어떻게 활용될 수 있는지에 대해서 전세계의 영화 공동제작 관련 네트워크 데이터를 사용하여 소개한다. This article introduces Exponential Random Graph Modeling (ERGM), a method for statistical inference in social network analysis. The following aspects of the method are explained in the text: 1) objectives, 2) model specification, 3) estimation, and 4) interpretation of the estimation results. I also provide an example case with empirical network data and show how ERGMs can be applied to examine real network data.

Bee Venom Reduces Atherosclerotic Lesion Formation via Anti-Inflammatory Mechanism.

Lee, Woo-Ram,Kim, Soo-Jung,Park, Ji-Hyun,Kim, Kyung-Hyun,Chang, Young-Chae,Park, Yoon-Yup,Lee, Kwang-Gill,Han, Sang-Mi,Yeo, Joo-Hong,Pak, Sok Cheon,Park, Kwan-Kyu Institute for Advanced Research in Asian Science a 2010 The American journal of Chinese medicine Vol.38 No.6

<P>The components of bee venom (BV) utilized in the current study were carefully scrutinized with chromatography. Despite its well documented anti-inflammatory property, there are no reports regarding the influence of BV on the expression of cellular adhesion molecules in the vascular endothelium. A great amount of information exists concerning the effects of an atherogenic diet on atherosclerotic changes in the aorta, but little is known about the molecular mechanisms and the levels of gene regulation involved in the anti-inflammatory process induced by BV. The experimental atherosclerosis was induced in mice by a lipopolysaccharide (LPS) injection and an atherogenic diet. The animals were divided into three groups, the NC groups of animals that were fed with a normal diet, the LPS/fat group was fed with the atherogenic diet and received intraperitoneal injections of LPS, and the LPS/fat + BV group was given LPS, an atherogenic diet and intraperitoneal BV injections. At the end of each treatment period, the LPS/fat + BV group had decreased levels of total cholesterol (TC) and triglyceride (TG) in their serum, compared to the LPS/fat group. The LPS/fat group had significant expression of tumor necrosis factor (TNF)-관 and interleukin (IL)-1관 in the serum, compared with the NC group (p < 0.05). The amount of cytokines reduced consistently in the BV treatment groups compared with those in LPS/fat group. BV significantly reduced the amount of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), transforming growth factor-관1 (TGF-관1) and fibronectin in the aorta, compared with the LPS/fat group (p < 0.05). A similar pattern was also observed in the heart. In conclusion, BV has anti-atherogenic properties via its lipid-lowering and anti-inflammatory mechanisms.</P>

Mannheimia succiniciproducens Phosphotransferase System for Sucrose Utilization

Lee, Jeong Wook,Choi, Sol,Kim, Ji Mahn,Lee, Sang Yup American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.5

<B>ABSTRACT</B><P>The succinic acid producer <I>Mannheimia succiniciproducens</I> can efficiently utilize sucrose as a carbon source, but its metabolism has not been understood. This study revealed that <I>M. succiniciproducens</I> uses a sucrose phosphotransferase system (PTS), sucrose 6-phosphate hydrolase, and a fructose PTS for the transport and utilization of sucrose.</P>

Catalytic characteristics of carbon black for decomposition of ethane

Lee, Sang Yup,Kim, Mi So,Kwak, Jung Hun,Han, Gui Young,Park, Jong Hyeok,Lee, Tae Jin,Yoon, Ki June Elsevier 2010 Carbon Vol.48 No.7

<P><B>Abstract</B></P><P>The catalytic activities of rubber, color and conductive carbon black catalysts for decomposition of ethane were investigated in the temperature range from 973 to 1173K. Significantly higher ethane conversion and lower ethylene selectivity were obtained in the presence of carbon black catalysts compared with non-catalytic decomposition, resulting in much higher hydrogen yields. This indicates that carbon black catalysts are effective catalysts for dehydrogenation of ethane to hydrogen and ethylene, as well as for the subsequent decomposition of ethylene to hydrogen and solid carbon. However, more methane was produced in the presence of carbon black catalysts than in non-catalytic decomposition. A reaction mechanism was proposed for the catalytic decomposition of ethane. The hydrogen yield increased with an increase in the specific surface area of the nonporous rubber and color carbon black catalysts with a surface area of up to approximately 100m<SUP>2</SUP>/g. However, the hydrogen yield over the carbon black catalysts with higher surface areas, including the conductive carbon black catalysts with very high surface areas, did not increase significantly. The carbon black catalysts exhibited stable activity for ethane decomposition and hydrogen production for 36h despite carbon deposition.</P>

<i>TP53</i> Mutations in Korean Patients with Non-small Cell Lung Cancer

Lee, Eung Bae,Jin, Guang,Lee, Shin Yup,Park, Ji Young,Kim, Min Jung,Choi, Jin Eun,Jeon, Hyo Sung,Cha, Seung Ick,Cho, Sukki,Kim, Chang Ho,Park, Tae-In,Jung, Tae Hoon,Son, Ji-Woong,Park, Jae Yong The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.5

<P>Although <I>TP53</I> mutations have been widely studied in lung cancer, the majority of studies have focused on exons 5-8 of the gene. In addition, <I>TP53</I> mutations in Korean patients with lung cancers have not been investigated. We searched for mutations in the entire coding exons, including splice sites of the gene, in Korean patients with non-small cell lung cancer (NSCLC). Mutations of the gene were determined by direct sequencing in 176 NSCLCs. Sixty-nine mutations (62 different mutations) were identified in 65 tumors. Of the 62 mutations, 12 were novel mutations. <I>TP53</I> mutations were more frequent in males, ever-smokers and squamous cell carcinomas than in females, never-smokers and adenocarcinomas, respectively (all comparisons, <I>P</I><0.001). Missense mutations were most common (52.2%), but frameshift, nonsense, and splice-site mutations were frequently observed at frequencies of 18.8%, 15.9% and 10.1%, respectively. Of the 69 mutations, 9 (13.0%) were found in the oligomerization domain. In addition, the proportion of mutations in the oligomerization domain was significantly higher in adenocarcinomas than in squamous cell carcinomas (23.5% vs. 2.9%, <I>P</I>=0.01). Our study provides clinical and molecular characteristics of <I>TP53</I> mutations in Korean patients with NSCLCs.</P>

TiO₂ nanotubes with a ZnO thin energy barrier for improved current efficiency of CdSe quantum-dot-sensitized solar cells

Lee, Wonjoo,Kang, Soon Hyung,Kim, Jae-Yup,Kolekar, Govind B,Sung, Yung-Eun,Han, Sung-Hwan IOP Pub 2009 Nanotechnology Vol.20 No.33

<P>This paper reports the formation of a thin ZnO energy barrier between a CdSe quantum dot (Q dots) sensitizer and TiO<SUB>2</SUB> nanotubes (TONTs) for improved current efficiency of Q dot-sensitized solar cells. The formation of a ZnO barrier between TONTs and the Q dot sensitizer increased the short-circuit current under illumination and also reduced the dark current in a dark environment. The power conversion efficiency of Q dot-sensitized TONT solar cells increased by 25.9% in the presence of the ZnO thin layer due to improved charge-collecting efficiency and reduced recombination. </P>

Efficacy of Sanitizing Treatments for Feline Calicivirus as a Norovirus Surrogate Attached to Food and Food Contact Surfaces

Lee, Sung-Young,Kim, Kwang-Yup The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.2

Norovirus (NV) is becoming a major cause of foodborne illness in many countries. At present, very little is known about the survival of NV in the environment or the disinfection procedures needed to remove NV from contaminated surfaces. Feline calicivirus (FCV, $1{\times}10^{6.75}\;TCID_{50}/mL$) was used as a surrogate model for NV to investigate the effectiveness of sanitizing treatments for the viruses attached to food and food contact surfaces. Ammonium chloride (2%), organic acids (3000 ppm), and ethanol (70%) were most effective, providing $4\;log_{10}$ (99.99%) reductions in FCV titers on food or food contact surfaces. The disinfection efficacies of most agents on ceramic and glass surfaces were greater than stainless steel. The results from this study can be applied in the food industry to reduce NV-associated foodborne illnesses.