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Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer
오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.
LEE, SUNG BOK,LEE, DEOK GOO,KWAG, JOO YOUNG,KIM, JUNG YEUL The Ophthalmic Communications Society, Inc. 2009 Retina Vol.29 No.8
PURPOSE:: To compare short-acting mydriatics versus long-acting mydriatics and to assess their effect on postoperative frequency and severity of posterior synechia after combined pars plana vitrectomy, phacoemulsification, and intraocular lens implantation. METHODS:: We retrospectively reviewed the records of 69 eyes of 69 patients who received a combined operation by the same surgeon for rhegmatogenous retinal detachment and cataracts. The mean follow-up period in both groups was 43 weeks. The frequency and severity of posterior synechia were analyzed at baseline and over a 6-month follow-up period. RESULTS:: Of the 69 eyes, 29.7% (11 of 37) in the long-acting mydriatic group versus 9.4% (3 of 32) in the short-acting mydriatic group had developed posterior synechia. The difference was statistically significant (P = 0.036). Additionally, when the severity of posterior synechia was measured in hour units, there was a statistically significant difference in severity between the 2 groups (long-acting mydriatic group, 0.76 ± 1.52 hours; short-acting mydriatic group, 0.13 ± 0.42 hours; P = 0.020). CONCLUSION:: The frequency and severity of posterior synechia after a combined operation may be reduced by the use of a short-acting mydriatic.
Lee, Sung Jun,Rim, Tyler Hyung Taek,Jang, Sun Young,Kim, Chan Yun,Shin, Dong Yeob,Lee, Eun Jig,Lee, Sang Yeul,Yoon, Jin Sook Springer-Verlag 2013 Graefe’s archive for clinical and experimental oph Vol.251 No.1
<P>To evaluate the efficacy of subconjunctival triamcinolone injection for treating upper eyelid retraction caused by thyroid-associated ophthalmopathy (TAO).</P>
Lee, Sang Wook,Ryu, Chae-Min,Shin, Jung-Hyun,Choi, Daeheon,Kim, Aram,Yu, Hwan Yeul,Han, Ju-Young,Lee, Hye-Yeon,Lim, Jisun,Kim, Yong Hwan,Heo, Jinbeom,Lee, Seungun,Ju, Hyein,Kim, Sujin,Hong, Ki-Sung,Ha Korean Continence Society 2018 International Neurourology Journal Vol.22 No.S1
<P><B>Purpose</B></P><P>To evaluate the therapeutic effect of human embryonic stem cell (hESC)-derived multipotent mesenchymal stem cells (M-MSCs) on ketamine-induced cystitis (KC) in rats.</P><P><B>Methods</B></P><P>To induce KC, 10-week-old female rats were injected with 25-mg/kg ketamine hydrochloride twice weekly for 12 weeks. In the sham group, phosphate buffered saline (PBS) was injected instead of ketamine. One week after the final injection of ketamine, the indicated doses (0.25, 0.5, and 1×10<SUP>6</SUP> cells) of M-MSCs (KC+M-MSC group) or PBS vehicle (KC group) were directly injected into the bladder wall. One week after M-MSC injection, the therapeutic outcomes were evaluated via cystometry, histological analyses, and measurement of gene expression. Next, we compared the efficacy of M-MSCs at a low dose (1×10<SUP>5</SUP> cells) to that of an identical dose of adult bone marrow (BM)-derived MSCs.</P><P><B>Results</B></P><P>Rats in the KC group exhibited increased voiding frequency and reduced bladder capacity compared to rats of the sham group. However, these parameters recovered after transplantation of M-MSCs at all doses tested. KC bladders exhibited markedly increased mast cell infiltration, apoptosis, and tissue fibrosis. Administration of M-MSCs significantly reversed these characteristic histological alterations. Gene expression analyses indicated that several genes associated with tissue fibrosis were markedly upregulated in KC bladders. However the expression of these genes was significantly suppressed by the administration of M-MSCs. Importantly, M-MSCs ameliorated bladder deterioration in KC rats after injection of a low dose (1×10<SUP>5</SUP>) of cells, at which point BM-derived MSCs did not substantially improve bladder function.</P><P><B>Conclusions</B></P><P>This study demonstrates for the first time the therapeutic efficacy of hESC-derived M-MSCs on KC in rats. M-MSCs restored bladder function more effectively than did BM-derived MSCs, protecting against abnormal changes including mast cell infiltration, apoptosis and fibrotic damage.</P>
Sang Hun Lee,Kyu-Yeul Lee,Yoonwhan Woo,Kang-Soo Lee 대한기계학회 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.2
The requirements of multi-resolution models of feature-based solids, which represent an object at many levels of feature detail, are increasing for engineering purposes, such as analysis, network-based collaborative design, virtual prototyping and manufacturing To provide multi-resolution models for various applications, it is essential to generate adequate solid models at varying levels of detail (LOD) after feature reariangement, based on the LOD criteria However, the non-commutative property of the union and subtraction Boolean operations is a severe obstacle to arbitrary feature rearrangement To solve this problem we propose history-based Boolean operations that satisfy the commutative law between union and subtraction operations by considering the history of the Boolean operations Because these operations guarantee the same resulting shape as the original and reasonable shapes at the intermediate LODs for an arbitrary rearrangement of its features, various LOD criteria can be applied for multi-resolution modeling in different applications.
Sang Hun Lee,Kyu-Yeul Lee,Yoonwhan Woo,Kang-Soo Lee 대한기계학회 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.2
We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive The multi-resolution solid representation proposed in this paper Includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entitles contributing to the LOD model shape.
A User-Oriented Interactive Model for the Conceptual Design of Bulk Cargo Ships
Lee, Dong-Kon,Lee, Kyung-Ho,Han, Soon-Hung,Lee, Soon-Sub,Lee, Kyu-Yeul,Shin, Soo-Chol,Shin, Dong-Won,Lee, Jong-Chol,Kwon, Sung-Chil The Society of Naval Architects of Korea 1994 Selected papers of the society of naval architects Vol.2 No.1
This paper describes a design model for the conceptual design of ships. Existing design models have problems such that their operating mode of batch versions cannot reflect the design procedures in reality. Reliability of the results is low because the performance estimations are based mainly on empirical formulas. To improve the problems of existing design models, a new design model has been developed. The new model consists of an interactive user interface, a database of main engines, a database of particulars of existing ships, and ten modules for performance estimations. To develop such a user-oriented system, the concept of graphical user interface (GUI) is adopted.
Cytochrome P450-mediated herb-drug interaction potential of Galgeun-tang
( Sang Yoon Lee ),( Ji Yoon Lee ),( Wonku Kang ),( Kwang-il-kwon ),( Song Kyu Park ),( Soo Jin Oh ),( Jin Yeul Ma ),( Sang Kyum Kim ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
We evaluated the herb-drug interaction potential of Galgeun-tang (GGT) extracts, mediated by cytochrome P450 (CYP) inhibition/induction. Further, the effects of fermentation on the CYP-mediated herb-drug interaction potential of GGT extracts were determined. As measured by LC-ESI/MS/MS, GGT extracts (0-300μg/mL) showed no inhibitory activity toward eight CYP isoforms (1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, and 3A4) in pooled human liver microsomes, suggesting that GGT may have low potential for herb-drug interactions mediated by CYP inhibition. Hepatic CYP expression and activity in rats treated with GGT extracts twice per day for 1week was examined. Among the tested CYP isoforms (1A1, 1A2, 1B1, 2B1, 2C11, 2E1, 3A1, 3A2, and 4A1), CYP1B1 and 4A1 were increased by GGT extracts. Hepatic activities of 7-ethoxyresorufin-O-deethylase, 7-pentoxyresorufin-O-depentylase, and chlorzoxazone 6-hydroxylase, but not midazolam hydroxylase were also elevated. These results raise the possibility that GGT extracts may increase the toxicity of environmental toxicants through the elevating CYP-dependent metabolic activation. Interestingly, the increases in CYP1B1 and CYP4A1 levels, and 7-ethoxyresorufin-O-deethylase, 7-pentoxyresorufin-O-depentylase, and chlorzoxazone 6-hydroxylase activities were attenuated by fermentation of GGT extract using Lactobacillus plantarum KFRI 402, but not 144. Further studies are needed to identify the CYP regulatory component(s) from GGT and determination its metabolism. ⓒ2012 Elsevier Ltd. All rights reserved.
Lee Sang Hun,Lee Kyu-Yeul,Woo Yoonwhan,Lee Kang-Soo The Korean Society of Mechanical Engineers 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.2
We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm. Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive. The multi-resolution solid representation proposed in this paper includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion. As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entities contributing to the LOD model shape.
Nursing Effects of Thiols Including Cysteine in Lymph Node Stromal Cells and P388 Cells
Lee, Sang-Han,Ma, Jin-Yeul,Park, Kap-Joo,Kang, Hyunmin,Park, Taekyu,Park, Doo-Sang Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.2
Mouse malignant T-lymphoma CS21 cells can grow when cocultured with CAl2 lymph node stromal cells, but they undergo apoptotic cell death with DNA fragmentation when separated from CA12 stromal cells. In the course of examining the effects of the soluble factor (s) secreted by CAl2 stromal cells on CS2l cell growth, we found that thiols including cysteine promoted CS2l cell growth. P388 cell growth was also promoted by various thiols. These results suggest that thiols including cysteine participate in CA12 and P388 cell growth.