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탱자(Poncirus tripoliata RAF)잎으로부터 Mitochondrial DNA의 분리
박종필,전홍성,김연순,이숙영,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-
Differaitial centrifugation, sucrose(30%, 60%) and percoll(13.5%, 21% and 45%) density gradient centrifugation were used to effectively isolate mitochondria and mitochondrial DNA (mt DNA) from the leaves of the genus Poncirus (Pondrus trifoliata RAF.) The results showed that percoll density gradient centrifugation provided a convinient and effective method for the preparation of clean mitochondria. The purity of prepared mt DNA was increased by the removal of the nuclear DNA by DNase treatment to the crude mitochondrial suspension mt DNA on the agarose gel was shown extra fluorescent background in case of isolation by differential centrifugation and more cleared band included slightly smeared patterns for sucrose density gradient, and highly paint band from percoll density gradient was appeared to the best results of the above three methods. In addition to chromosomal mt DNAs, two short mt plasmid-like elements were detected in the genus Poncirus. These elements consistently co-purified with the chromosomal mt DNAs and is widely distributed among natrual Poncirus trifoliata RAF. populations. But these elements required proteinase K treatment for mobility in agarose gels.
천마분말이 고콜레스테롤혈증 흰쥐 혈청의 지질성분에 미치는 영향
박미연,성낙주,신정애,이수정,박필숙 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.1
This study was designed to investigate the optimum recommendation level on Gastrodia rhizoma and the effects on the improvement of the lipids in the dietary hypercholesterolemic rats. Experimental diets mixed with 5% Dioscorea batatas(Group 2), 10% Dioscorea batatas(Group 3), 15% Dioscorea batatas(Group 4), 5% Gastrodia rhizoma(Group 5), 10% Gastrodia rhizoma (Group 6) and 15% Gastrodia rhizoma(Group 7) were administered to the male rats of the Sprague Dawley for 3 weeks. Concentration of total cholesterol in serum was lower in the Gastrodia rhizoma groups than in the other groups, especially total cholesterol concentration of 10% Gastrodia rhizoma(Group 6) was the lowest in the Gastrodia rhizoma groups. Concentration of HDL-cholesterol in serum was higher in the 10% Gastrodia rhizoma and 15% Gastrodia rhizoma than in the other groups. Concentrations of cholesterylester, LDL, LDL-cholesterol in serum were the lowest in the 10% Gastrodia rhizoma. Concentration of glucose and activity of GPT in serum were the lowest in the 10% Gastrodia rhizoma group. The activity of GOT in serum was lower in the 10% Gastrodia rhizoma group and 15% Gastrodia rhizoma group than in the other groups. Therefore, we consider that there are effects on the improvement of the lipids in the Gastrodia rhizoma and the optimum recommendation quantity of Gastrodia rhizoma is 10% to quantity of food composition.
마분말과 천마분말이 흰쥐의 간, 뇌 및 신장의 지질성분 변화에 미치는 영향
박필숙,성낙주,박미연 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2
The present study tested to recommend optimum level of Gastrodia rhizoma and effect on the compositional changes of the lipids in Sprague Dawley rats which were fed control diet adding 5, 10, 15% Dioscorea batatas powder and 5, 10, 15% Gastrodia rbizoma powder for 3 weeks, respectively, The contents of moisture, ash, fat, protein and carbohydrate in dried Gastrodia rbizoma were 5.9, 2.7, 2.1, 0.9 and 88.4%(w/w) respectively. Concentrations of total cholesterol and free cholesterol in liver were the lowest in the 10% Gastrodia rbizoma group. Concentrations of triglyceride and phospholipid in liver were lower in the 10 and 15% Gastrodia rhizoma groups than in the other groups. Concentrations of total cholesterol, free cholesterol, triglyceride and phospholipid in brain were significantly lower in the group 6, 7 compared to the other groups. Concentrations of triglyceride and phospholipid in kidney were slightly lower in the Gastrodia rhizoma groups than those in the Dioscorea batatas groups.
성연상,김상진,박찬용,박순필,도종웅 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6
The authors surveyed one-hundred women whithin 24-36 hours after delivary for the evaluation of low back pain in pregnancy. It showed that 55% of them suffered from low back pain during pregnancy. Low back pains mostly started form 6-9 months of gestational age. Low back pains were tolerable at 84% of them. And low back pains were not aggravated during pregnancy among the 67% of low back pain pregnant women. Leg pains were noted at 34% of low back pain group.
저장기간에 따른 오이김치의 비타민 C 및 클로로필 함량변화
박미란,이연정,고즈쿠에 노부유키,한재숙,최석현,허성미,한경필,최수근 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.5
This study was carried out to examine the changes of pH, chlorophyll, chlorophyll patterns and ascorbic acid contents, and sensory evaluation in Oi-Kimchi by the different periods of storage. The contents of chlorophyll a and b were 52.2% and 14.44% at storage 0 days, 20.67% and 16.99% at storage 11 days, 0% at storage 14 days, and decreased with storage times increased. But, phephorbide a and b were increased with storage times increased until storage 11 days, and were 28.44% and 1.25% at storage 0 days, 42.63% and 15.79% at storage 11 days, 0% at storage 12, 14 days. The contents of ascorbic acid were increased until storage 4 days, but afterwards were decreased with storage times increased, and were 2.57㎎% at storage 0 days, 5.49㎎% and 2.95㎎% at storage 11 days, 0% at storage 14 days. Sensory evaluations of appearance, flavor, taste, texture, overall acceptability showed the highest at storage 8 days, and disliked after storage 10 days.
소뇌에 발생한 Nocardiosis : 증례 보고 Case Report
성연상,김상진,박순필,강재규,도종웅 대한신경외과학회 1989 Journal of Korean neurosurgical society Vol.18 No.6
The authors report a extemely rare case of primary cerebellar nocardial abscess. Cerebral nocardiosis is rare disease and often fatal, and often fatal, and commonly spreaded from nocardiosis of lung or skin. Total excision of abscess and medication with sulfonamide is the treatment of choice. The patient was treated by continuous external dirainage with intermittent irrigation of abscess cavity by sulfonamide and given with sulfonamide for 10 months.
뇌하수체 부위의 결핵성 뇌농양 : 증례 보고1예 A Case Report
박수영,박순필,김상진,도종웅,주양자 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.1
The authors described a case of tuberculous brain abscess, located on the sellar area with an episode of tuberculous meningitis on the past history. Tuberculous brain abscess, encapsulated collection of pus, is quite rare. Especially, occurrence around the sellar area is extremely rare. On brain CT scan, tuberculous brain abscess on sellar region was differented from pituitary adenoma, craniopharyngioma, germinoma, pyogenic brain abscess, etc. The history of previous tuberculous meningitis was meaningful for the diagnosis of our case. So, we have reported this case with review of the articles.