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Proteome analysis of outer membrane vesicles from a clinical<i>Acinetobacter baumannii</i>isolate
Kwon, Sang-Oh,Gho, Yong Song,Lee, Je Chul,Kim, Seung Il Oxford University Press 2009 FEMS microbiology letters Vol.297 No.2
<P>The secretion of outer membrane vesicles (OMVs) is one of the major mechanisms by which Gram-negative bacteria deliver effector molecules to host cells. Acinetobacter baumannii is an important opportunistic pathogen in hospital-acquired infections, but the secretion system for effector molecules to induce host cell damage has not been characterized. In the present study, we investigated the secretion of OMVs from a clinical A. baumannii isolate and analyzed the comprehensive proteome of A. baumannii-derived OMVs. Acinetobacter baumannii secreted OMVs into the extracellular milieu during in vitro growth. Using 1-DE and LC-MS/MS protein identification and assignment analysis, 132 different proteins associated with OMVs were identified. These proteins were derived from outer membranes (n=26), periplasmic space (n=6), inner membranes (n=8), cytoplasm (n=43), and unknown localization or multiple localization sites (n=49) according to the cell location prediction programs. Among the proteins associated with OMVs, a potent cytotoxic molecule, outer membrane protein A, was highly enriched and several putative virulence-associated proteins were also identified. These results suggest that OMVs from A. baumannii are an important vehicle designed to deliver effector molecules to host cells.</P>
(Sung Hyun Kwon),(Dae Chul Cho) 한국물환경학회 1999 한국물환경학회지 Vol.15 No.3
The biodegradation of one popular nitramine energetics, Ammonium Dinitramide (ADN) by mixture of dentrifying bacterial species was investigated. ADN was observed to be effectively mineralized in the anaerobic mixed culture. The initial ADN concentration of 250㎎/L was reduced to non-detectable levels (>99% removal efficiency) in 5 days of incubation under anaerobic conditions. Final products generated from anaerobic degradation of nitramine energetics by anaerobic metabolism were NH₄^+, CH₄, and CO₂ that were released to the environment with the denitrifiers` growth. In addition, it was found that the activity of denitrifiers was inhibited by high concentration of ammonia generated through the degradation reactions of energetic nitrites.
(Sung Hyun Kwon),(Duk Soo Choi),(Dae Chul Cho) 한국물환경학회 2000 한국물환경학회지 Vol.16 No.1
Biodegradability of pentachlorophenol and its biological kinetic parameters were investigated using a chemical oxygen demand (COD) analysis. The experiments were performed in continuous stirred tank reactors with synthetic wastewater containing 30 and 60㎎/L of pentachlorophenol (PCP) and other carbon sources. Mean cell residence time (MCRT) and hydraulic residence time (HRT) were maintained from 4.8 to 26.2 days and 4 to 17 hours, respectively. While synthetic wastewater containing 30 ㎎/L of PCP showed over 90% S-COD removal efficiencies except at the 4.8 and 20.3 days of MCRT, 55% to 75% removal efficiencies were observed for 60 ㎎/L of PCP concentration. The respective values of Y and k_d were 0.15 and 0.010 day^(-1) for 30 ㎎/L PCP (r²= 0.90), and 0.13 and 0.014 day^(-1) for 60 ㎎/L PCP (r² = 0.86), respectively. In a substrate inhibition kinetics, Haldane model, the respective values of K_s, K_i and k were 60.8 ㎎ COD/L, 297.5 ㎎ COD/L and 0.93 day^(-1) for 30 ㎎/L PCP (r² = 0.30), and 262.2 ㎎ COD/L, 997.2 ㎎ COD/L and 0.053 day^(-1) for 60 ㎎/L PCP (r² = 0.78), respectively.
Effect of Chitooligosaccharides on Antioxidative Enzyme Activity in Liver of Rat Fed High Fat Diet
( Sang Chul Kwon ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.4
In this study, the antioxidant activity of chitooligosaccharides (COSs) less than 10 kDa in molecular weight was characterized in the liver of rats fed a high fat diet (n=5, age 4 weeks). The enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and catalase (CAT) in the liver were measured after feeding rat a high fat diet (high fat group) or a high fat diet supplemented with 0.2 g of COSs per kg of rat body weight for 4 weeks. The activity of SOD, GPx, GST and CAT for the COSs group was higher than that of high fat group. These results indicate that the COSs of less than 10 kDa have potent antioxidant activity in rats fed with high fat diet.
Kwon, Dong Rak,Park, Gi-Young,Lee, Sang Chul Hindawi 2018 Stem cells international Vol.2018 No.-
<P><B>Objective</B></P><P> The aim of this study was to investigate regenerative effects of ultrasound- (US-) guided injection with human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) and/or polydeoxyribonucleotide (PDRN) injection in a chronic traumatic full-thickness rotator cuff tendon tear (FTRCTT) in a rabbit model. </P><P><B>Methods</B></P><P> Rabbits (<I>n</I> = 32) were allocated into 4 groups. After a 5 mm sized FTRCTT just proximal to the insertion site on the subscapularis tendon was created by excision, the wound was immediately covered by a silicone tube to prevent natural healing. After 6 weeks, 4 injectants (0.2 mL normal saline, G1-SAL; 0.2 mL PDRN, G2-PDRN; 0.2 mL UCB-MSCs, G3-MSC; and 0.2 mL UCB-MSCs with 0.2 ml PDRN, G4-MSC + PDRN) were injected into the FTRCTT under US guidance. We evaluated gross morphologic changes on all rabbits after sacrifice. Masson's trichrome, anti-type 1 collagen antibody, bromodeoxyuridine, proliferating cell nuclear antigen, vascular endothelial growth factor, and platelet endothelial cell adhesion molecule stain were performed to evaluate histological changes. Motion analysis was also performed. </P><P><B>Results</B></P><P> The gross morphologic mean tendon tear size in G3-MSC and G4-MSC + PDRN was significantly smaller than that in G1-SAL and G2-PDRN (<I>p</I> < 0.05). However, there were no significant differences in the tendon tear size between G3-MSC and G4-MSC + PDRN. In G4-MSC + PDRN, newly regenerated collagen type 1 fibers, proliferating cell activity, angiogenesis, walking distance, fast walking time, and mean walking speed were greater than those in the other three groups on histological examination and motion analysis. </P><P><B>Conclusions</B></P><P> Coinjection of UCB-MSCs and PDRN was more effective than UCB-MSC injection alone in histological and motion analysis in a rabbit model of chronic traumatic FTRCTT. However, there was no significant difference in gross morphologic change of tendon tear between UCB-MSCs with/without PDRN injection. The results of this study regarding the combination of UCB-MSCs and PDRN are worth additional investigations.</P>
Intraoperative Discography for Detecting Concealed Lumbar Discal Cysts
Kwon, Yoon-Kwang,Choi, Kyung-Chul,Lee, Choon Dae,Lee, Sang-Ho The Korean Neurosurgical Society 2013 Journal of Korean neurosurgical society Vol.53 No.4
Lumbar discal cyst is a rare cause of radiculopathy. Their exact pathogenesis and the optimal treatment modality remain unidentified. Depending on their location, discal cysts cannot always be easily identified intraoperatively. We describe 2 patients with discal cysts and introduce an intraoperative discography technique for discal cyst location. Both patients were treated with surgical excision; with intraoperative discography, the cystic lesions could easily be detected and removed.
Kwon, Jae-Yoel,Sung, Jae-Hoon,Kim, Il-Sup,Son, Byung-Chul The Korean Neurosurgical Society 2011 Journal of Korean neurosurgical society Vol.50 No.3
Authors report a case of a painless acute dissecting aneurysm of the descending aorta in a patient who presented with unexplained hypotension followed by simultaneous paraplegia and right arm monoparesis. To our knowledge, case like this has not been reported previously. Magnetic resonance imaging of the brain and spine revealed hemodynamic cerebral infarction and extensive cord ischemia, respectively. Computerized tomography angiography confirmed a dissecting aneurysm of the descending aorta. The cause of the brain infarction may not have been embolic, but hemodynamic one. Dissection-induced hypotension may have elicited cerebral perfusion insufficiency. The cause of cord ischemia may be embolic or hemodynamic. The dissected aorta was successfully replaced into an artificial patch graft. The arm monoparesis was improved, but the paraplegia was not improved. In rare cases of brain and/or spinal cord infarction caused by painless acute dissecting aneurysm of the aorta, accurate diagnosis is critical because careless thrombolytic therapy can result in life-threatening bleeding.